Name: Aquicella siphonis SGT-108
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 3247

Type strain:

Species: Aquicella siphonis

Strain Designation: SGT-108

Culture col. no.: CCUG 48881, DSM 17428, CIP 107651, LMG 21648

Strain history: CIP <- 2002, Milton S. Da Costa, Coimbra, Portugal: strain SGT-108

NCBI tax ID(s): 254247 (species)

SI-ID 88962

LMG 21648, CIP 107651, CCUG 48881, DSM 17428

Section

Aquicella siphonis SGT-108 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from water at thermal spa.

Gram-negative
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Legionellales*
	Family	*Coxiellaceae*
	Genus	*Aquicella*
	Species	**Aquicella siphonis**
	Full Scientific Name (LPSN)	Aquicella siphonis Santos et al. 2004

Information on morphological and physiological properties Morphology

[Ref.: #122637]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %100

[Ref.: #122637]	Cell shape	rod-shaped

[Ref.: #122637]	Motility	no

[Ref.: #58378]

Incubation period

3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6963]

Culture medium

BCYE-AGAR (DSMZ Medium 585)

[Ref.: #6963]

Culture medium growth

[Ref.: #6963]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6963]

Culture medium composition

expand / minimize

[Ref.: #39917]

Culture medium

MEDIUM 23 - for Afipia and Legionella

[Ref.: #39917]

Culture medium growth

[Ref.: #39917]

Culture medium composition

expand / minimize

[Ref.: #122637]

Culture medium

CIP Medium 23

[Ref.: #122637]

Culture medium growth

[Ref.: #122637]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #6963]	growth	37 °C
[Ref.: #39917]	growth	37 °C
[Ref.: #58378]	growth	37 °C

[Ref.: #6963]	Temperature range	mesophilic
[Ref.: #39917]	Temperature range	mesophilic
[Ref.: #58378]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #58378]

Oxygen tolerance

microaerophile

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.999

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #122637]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #122637]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122637]	10539	-	-	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6963]	Sample type/isolated from	water at thermal spa
[Ref.: #6963]	Geographic location (country and/or sea, region)	Sao Gemil
[Ref.: #6963]	Country	Portugal
[Ref.: #6963]	Country ISO 3 Code	PRT
[Ref.: #6963]	Continent	Europe

[Ref.: #58378]	Sample type/isolated from	Water,borehole at spa
[Ref.: #58378]	Country	Portugal
[Ref.: #58378]	Country ISO 3 Code	PRT
[Ref.: #58378]	Continent	Europe

[Ref.: #122637]	Sample type/isolated from	Environment, Borehole water at spa
[Ref.: #122637]	Country	Portugal
[Ref.: #122637]	Country ISO 3 Code	PRT
[Ref.: #122637]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY359283 (>99% sequence identity) for Aquicella siphonis subclade from Microbeatlas

Aquatic Samples	27
Soil Samples	67
Animal Samples	3
Plant Samples	1
Total Samples	98

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6963]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122637]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6963]	Aquicella siphonis strain SGT-108T 16S ribosomal RNA gene, partial sequence	AY359283	1482	ENA	254247 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Aquicella siphonis SGT-108	GCA_902459485	complete	GenBank	254247 tax ID	*
[Ref.: #66792]	Aquicella siphonis strain SGT-108	254247.3	complete	PATRIC	254247 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Aquicella siphonis SGT-108 NCBI: GCA_902459485.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Aquicella siphonis SGT-108 NCBI: GCA_902459485.1
[Ref.: #69480]	flagellated	no	72.706	no
[Ref.: #69480]	gram-positive	no	98.999	no
[Ref.: #69480]	anaerobic	no	97.345	yes
[Ref.: #69480]	aerobic	no	87.758	yes
[Ref.: #69480]	halophile	no	85.246	no
[Ref.: #69480]	spore-forming	no	93.984	no
[Ref.: #69480]	thermophile	no	92.624	yes
[Ref.: #69480]	glucose-util	yes	64.382	no
[Ref.: #69480]	motile	yes	74.464	no
[Ref.: #69480]	glucose-ferment	no	83.533	no

Availability in culture collections External links

[Ref.: #6963]

Culture collection no.

CCUG 48881, DSM 17428, CIP 107651, LMG 21648

[Ref.: #72760]

SI-ID 88962

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Gamma-proteobacteria Aquicella lusitana gen. nov., sp. nov., and Aquicella siphonis sp. nov. infect protozoa and require activated charcoal for growth in laboratory media.	Santos P, Pinhal I, Rainey FA, Empadinhas N, Costa J, Fields B, Benson R, Verissimo A, Da Costa MS	Appl Environ Microbiol	10.1128/AEM.69.11.6533-6540.2003	2003	*

References

#6963

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17428

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#39917 ; Curators of the CIP;
#58378 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 48881

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#72760

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID88962.1 )

#122637 Collection of Institut Pasteur ; Curators of the CIP; CIP 107651
* These data were automatically processed and therefore are not curated

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