Name: Acinetobacter sichuanensis WCHAc060041
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159774

Type strain:

Species: Acinetobacter sichuanensis

Strain Designation: WCHAc060041

Culture col. no.: CCTCC AB 2018118, GDMCC 1.1383, KCTC 62575

Strain history: <- Qin Jiayuan, West China Hospital

NCBI tax ID(s): 2136183 (species)

Section

Acinetobacter sichuanensis WCHAc060041 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from hospital sewage.

coccus-shaped
colony-forming
Gram-negative
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Moraxellaceae*
	Genus	*Acinetobacter*
	Species	**Acinetobacter sichuanensis**
	Full Scientific Name (LPSN)	Acinetobacter sichuanensis Qin et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66784]	Gram stain	negative

[Ref.: #66784]	Cell shape	coccus-shaped

[Ref.: #66784]	Motility	no

[Ref.: #66784]	Type of hemolysis	gamma
[Ref.: #66784]	Colony size	1-2 mm
[Ref.: #66784]	Colony color	light yellow
[Ref.: #66784]	Colony shape	circular
[Ref.: #66784]	Incubation period	1 day
[Ref.: #66784]	Cultivation medium used	BHI agar

Information on culture and growth conditions Culture and growth conditions

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Culture medium

BHI agar

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Culture medium growth

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Culture medium

LB (Luria-Bertani) agar

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Culture medium growth

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Culture medium

Müller-Hinton agar

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Culture medium growth

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Culture medium

tryptic soy agar (TSA)

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Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #66784]			growth	25-32 °C
[Ref.: #67771]			growth	30 °C

[Ref.: #66784]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #66784]			growth	5-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66784]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

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Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66784]		NaCl		growth	0-2	%(w/v)

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Observation

Cells are incapable of swimming motility.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66784]	(R)-malic acid ChEBI	+	carbon source
[Ref.: #66784]	2,3-butanediol ChEBI	+	carbon source
[Ref.: #66784]	4-aminobutyrate ChEBI	+	carbon source
[Ref.: #66784]	4-hydroxybenzoate ChEBI	+	carbon source
[Ref.: #66784]	4-oxopentanoate ChEBI	+	carbon source
[Ref.: #66784]	acetic acid ChEBI	+	carbon source
[Ref.: #66784]	adipate ChEBI	+	carbon source
[Ref.: #66784]	azelaate ChEBI	+	carbon source
[Ref.: #66784]	benzoate ChEBI	+	carbon source
[Ref.: #66784]	beta-alanine ChEBI	+	carbon source
[Ref.: #66784]	citraconate ChEBI	-	growth
[Ref.: #66784]	citric acid ChEBI	-	growth
[Ref.: #66784]	D-gluconate ChEBI	-	growth
[Ref.: #66784]	D-glucose ChEBI	-	builds acid from
[Ref.: #66784]	D-glucose ChEBI	-	growth
[Ref.: #66784]	D-ribose ChEBI	-	growth
[Ref.: #66784]	ethanol ChEBI	+	carbon source
[Ref.: #66784]	gelatin ChEBI	-	hydrolysis
[Ref.: #66784]	gentisate ChEBI	+	carbon source
[Ref.: #66784]	glutarate ChEBI	+	carbon source
[Ref.: #66784]	histamine ChEBI	-	growth
[Ref.: #66784]	L-arabinose ChEBI	-	growth
[Ref.: #66784]	L-arginine ChEBI	-	growth
[Ref.: #66784]	L-glutamate ChEBI	+	carbon source
[Ref.: #66784]	L-histidine ChEBI	-	growth
[Ref.: #66784]	L-leucine ChEBI	-	growth
[Ref.: #66784]	L-ornithine ChEBI	-	growth
[Ref.: #66784]	L-phenylalanine ChEBI	+	carbon source
[Ref.: #66784]	L-tartrate ChEBI	+	carbon source
[Ref.: #66784]	lactate ChEBI	+	carbon source
[Ref.: #66784]	malonate ChEBI	-	growth
[Ref.: #66784]	phenylacetate ChEBI	+	carbon source
[Ref.: #66784]	putrescine ChEBI	+	carbon source
[Ref.: #66784]	trans-aconitate ChEBI	-	growth
[Ref.: #66784]	tricarballylate ChEBI	-	growth
[Ref.: #66784]	trigonelline ChEBI	-	growth
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic resistance	Concentration
[Ref.: #66784]		meropenem ChEBI	yes	32	µg/mL

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66784]	Sample type/isolated from	hospital sewage
[Ref.: #66784]	Sampling date	2017-11
[Ref.: #66784]	Geographic location (country and/or sea, region)	West China Hospital of Sichuan University in Chengdu, Sichuan Province
[Ref.: #66784]	Country	China
[Ref.: #66784]	Country ISO 3 Code	CHN
[Ref.: #66784]	Continent	Asia
[Ref.: #66784]	Enrichment culture	Acinetobacter chromogenic agar plate
[Ref.: #66784]	Enrichment culture composition	containing 4 µg ml-1 meropenem and 32 µg ml-1 linezolid. Meropenem was added to screen carbapenem-resistant isolates, while linezolid was added to inhibit the growth of Gram-positive bacteria.
[Ref.: #66784]	Enrichment culture duration	overnight
[Ref.: #66784]	Enrichment culture temperature	30 ̊C
[Ref.: #66784]	Isolation/enrichment procedure	Sewage sample was added in 10 ml nutrient broth (Oxoid) and was incubated overnight at 30°C with shaking. The culture suspension was diluted to the 0.5 McFarland standard and was further diluted in 1 : 100 with saline. A 100 µl aliquot was streaked onto an Acinetobacter chromogenic agar plate and incubated at 30°C overnight.

[Ref.: #67771]	Sample type/isolated from	From sewage, West China Hospital
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Waste	#Sewage sludge
#Environmental	#Aquatic	-
#Infection	#Medical environment	#Clinic

What are isolation sources categories?

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Global distribution of 16S sequence MH179327 (>99% sequence identity) for Acinetobacter from Microbeatlas

Aquatic Samples	107
Soil Samples	16
Animal Samples	153
Plant Samples	32
Total Samples	308

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66784]	Acinetobacter sp. strain WCHAc060041 16S ribosomal RNA gene, partial sequence	MH179327	1525	ENA	472 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Acinetobacter sichuanensis WCHAc060041	GCA_003024515	contig	GenBank	2136183 tax ID	*
[Ref.: #66792]	Acinetobacter sichuanensis WCHAc060041	2814123059	draft	JGI IMG/M	2136183 tax ID	*

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GC-content

37.02 mol%

genome sequence analysis

Availability in culture collections External links

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Culture collection no.

CCTCC AB 2018118, GDMCC 1.1383, KCTC 62575

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Acinetobacter sichuanensis sp. nov., recovered from hospital sewage in China.	Qin J, Hu Y, Feng Y, Lv X, Zong Z	Int J Syst Evol Microbiol	10.1099/ijsem.0.003086	2018	*
Phylogeny	Acinetobacter faecalis Sp. Nov., Isolated from Elephant Faeces.	Chen XM, An DF, He SR, Yang SJ, Yang ZZ, Xiong LS, Li GD, Jiang MG, Jiang CL, Jiang Y	Curr Microbiol	10.1007/s00284-022-03097-9	2022	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66784

Jiayuan Qin, Yiyi Hu, Yu Feng, Xiaoju Lv, Zhiyong Zong: Acinetobacter sichuanensis sp. nov., recovered from hospital sewage in China. IJSEM 68: 3897 - 3901 2018 ( DOI 10.1099/ijsem.0.003086 , PubMed 30387708 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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