Name: Alicyclobacillus montanus USBA-GBX-503
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158721

Type strain:

Species: Alicyclobacillus montanus

Strain Designation: USBA-GBX-503

Culture col. no.: CMPUJ UGB U503, CBMAI 1927

NCBI tax ID(s): 1830138 (species)

Section

Alicyclobacillus montanus USBA-GBX-503 is an autotroph, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from an acidic thermal spring.

colony-forming
Gram-positive
motile
spore-forming
autotroph
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Alicyclobacillaceae*
	Genus	*Alicyclobacillus*
	Species	**Alicyclobacillus montanus**
	Full Scientific Name (LPSN)	Alicyclobacillus montanus López et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #65468]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %99.676

[Ref.: #65468]	Cell length	3-5 µm

[Ref.: #65468]	Cell width	0.5-1.0 µm

[Ref.: #65468]	Motility	yes

[Ref.: #65468]	Colony size	1-2 mm
[Ref.: #65468]	Colony color	beige
[Ref.: #65468]	Colony shape	circular

Information on culture and growth conditions Culture and growth conditions

	Temperatures	Kind of temperature		Temperature
[Ref.: #65468]			growth	25-55 °C
[Ref.: #65468]			optimum	45 °C

[Ref.: #65468]	Temperature range	thermophilic
[Ref.: #69480]	Temperature range	thermophilic Confidence in %99.54

	pH	Kind of pH		pH
[Ref.: #65468]			optimum	3-4
[Ref.: #65468]			optimum	1.5-4.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65468]	Nutrition type	autotroph
[Ref.: #65468]	Nutrition type	heterotroph
[Ref.: #65468]	Nutrition type	mixotroph

[Ref.: #65468]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %97.315

[Ref.: #65468]	Observation	final electron acceptor is Fe3+ under anaerobic conditions and oxidizes Fe2+ under aerobic conditions in the presence of organic and inorganic carbon sources
[Ref.: #65468]	Observation	requiring an inorganic source of electrons (Fe2+) for growth
[Ref.: #65468]	Observation	only respiratory quinone found is MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65468]	cellobiose ChEBI	+	builds acid from
[Ref.: #65468]	D-fructose ChEBI	+	builds acid from
[Ref.: #65468]	D-galactose ChEBI	+	builds acid from
[Ref.: #65468]	glucose ChEBI	+	builds acid from
[Ref.: #65468]	glycerol ChEBI	+	builds acid from
[Ref.: #65468]	L-arabinose ChEBI	+	builds acid from
[Ref.: #65468]	lactose ChEBI	+	builds acid from
[Ref.: #65468]	melibiose ChEBI	+	builds acid from
[Ref.: #65468]	rhamnose ChEBI	+	builds acid from
[Ref.: #65468]	trehalose ChEBI	+	builds acid from

	Enzyme	Enzyme activity	EC number
[Ref.: #65468]	acid phosphatase	+	3.1.3.2
[Ref.: #65468]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #65468]	alpha-galactosidase	+	3.2.1.22
[Ref.: #65468]	alpha-glucosidase	+	3.2.1.20
[Ref.: #65468]	beta-galactosidase	-	3.2.1.23
[Ref.: #65468]	beta-glucosidase	+	3.2.1.21
[Ref.: #65468]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65468]	catalase	+	1.11.1.6
[Ref.: #65468]	cystine arylamidase	-	3.4.11.3
[Ref.: #65468]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65468]	esterase (C 4)	+
[Ref.: #65468]	esterase Lipase (C 8)	+
[Ref.: #65468]	leucine arylamidase	+	3.4.11.1
[Ref.: #65468]	lipase (C 14)	-
[Ref.: #65468]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65468]	trypsin	+	3.4.21.4
[Ref.: #65468]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	cellulose degradation	100	5 of 5
[Ref.: #66794]	L-lactaldehyde degradation	100	3 of 3
[Ref.: #66794]	vitamin K metabolism	100	5 of 5
[Ref.: #66794]	valine metabolism	100	9 of 9
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	palmitate biosynthesis	100	22 of 22
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	cardiolipin biosynthesis	100	7 of 7
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	gluconeogenesis	87.5	7 of 8
[Ref.: #66794]	C4 and CAM-carbon fixation	87.5	7 of 8
[Ref.: #66794]	isoleucine metabolism	87.5	7 of 8
[Ref.: #66794]	tetrahydrofolate metabolism	85.71	12 of 14
[Ref.: #66794]	reductive acetyl coenzyme A pathway	85.71	6 of 7
[Ref.: #66794]	phenylalanine metabolism	84.62	11 of 13
[Ref.: #66794]	leucine metabolism	84.62	11 of 13
[Ref.: #66794]	glycolate and glyoxylate degradation	83.33	5 of 6
[Ref.: #66794]	methionine metabolism	80.77	21 of 26
[Ref.: #66794]	glycine betaine biosynthesis	80	4 of 5
[Ref.: #66794]	glycogen metabolism	80	4 of 5
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	threonine metabolism	80	8 of 10
[Ref.: #66794]	methylglyoxal degradation	80	4 of 5
[Ref.: #66794]	photosynthesis	78.57	11 of 14
[Ref.: #66794]	heme metabolism	78.57	11 of 14
[Ref.: #66794]	serine metabolism	77.78	7 of 9
[Ref.: #66794]	molybdenum cofactor biosynthesis	77.78	7 of 9
[Ref.: #66794]	NAD metabolism	77.78	14 of 18
[Ref.: #66794]	vitamin B1 metabolism	76.92	10 of 13
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	sulfopterin metabolism	75	3 of 4
[Ref.: #66794]	pentose phosphate pathway	72.73	8 of 11
[Ref.: #66794]	citric acid cycle	71.43	10 of 14
[Ref.: #66794]	glutamate and glutamine metabolism	71.43	20 of 28
[Ref.: #66794]	vitamin B12 metabolism	70.59	24 of 34
[Ref.: #66794]	starch degradation	70	7 of 10
[Ref.: #66794]	4-hydroxyphenylacetate degradation	70	7 of 10
[Ref.: #66794]	propionate fermentation	70	7 of 10
[Ref.: #66794]	isoprenoid biosynthesis	69.23	18 of 26
[Ref.: #66794]	degradation of sugar alcohols	68.75	11 of 16
[Ref.: #66794]	CO2 fixation in Crenarchaeota	66.67	6 of 9
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	flavin biosynthesis	66.67	10 of 15
[Ref.: #66794]	enterobactin biosynthesis	66.67	2 of 3
[Ref.: #66794]	purine metabolism	65.96	62 of 94
[Ref.: #66794]	histidine metabolism	65.52	19 of 29
[Ref.: #66794]	glycolysis	64.71	11 of 17
[Ref.: #66794]	glutathione metabolism	64.29	9 of 14
[Ref.: #66794]	tryptophan metabolism	63.16	24 of 38
[Ref.: #66794]	ketogluconate metabolism	62.5	5 of 8
[Ref.: #66794]	dTDPLrhamnose biosynthesis	62.5	5 of 8
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
[Ref.: #66794]	urea cycle	61.54	8 of 13
[Ref.: #66794]	degradation of hexoses	61.11	11 of 18
[Ref.: #66794]	polyamine pathway	60.87	14 of 23
[Ref.: #66794]	phenylacetate degradation (aerobic)	60	3 of 5
[Ref.: #66794]	lipoate biosynthesis	60	3 of 5
[Ref.: #66794]	alanine metabolism	58.62	17 of 29
[Ref.: #66794]	pyrimidine metabolism	57.78	26 of 45
[Ref.: #66794]	lysine metabolism	57.14	24 of 42
[Ref.: #66794]	propanol degradation	57.14	4 of 7
[Ref.: #66794]	d-mannose degradation	55.56	5 of 9
[Ref.: #66794]	carotenoid biosynthesis	54.55	12 of 22
[Ref.: #66794]	proline metabolism	54.55	6 of 11
[Ref.: #66794]	degradation of pentoses	53.57	15 of 28
[Ref.: #66794]	non-pathway related	52.63	20 of 38
[Ref.: #66794]	lipid metabolism	51.61	16 of 31
[Ref.: #66794]	Entner Doudoroff pathway	50	5 of 10
[Ref.: #66794]	toluene degradation	50	2 of 4
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	pantothenate biosynthesis	50	3 of 6
[Ref.: #66794]	coenzyme M biosynthesis	50	5 of 10
[Ref.: #66794]	aminopropanol phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	arginine metabolism	50	12 of 24
[Ref.: #66794]	cysteine metabolism	50	9 of 18
[Ref.: #66794]	phenylmercury acetate degradation	50	1 of 2
[Ref.: #66794]	phenylpropanoid biosynthesis	46.15	6 of 13
[Ref.: #66794]	sulfate reduction	46.15	6 of 13
[Ref.: #66794]	cholesterol biosynthesis	45.45	5 of 11
[Ref.: #66794]	oxidative phosphorylation	43.96	40 of 91
[Ref.: #66794]	androgen and estrogen metabolism	43.75	7 of 16
[Ref.: #66794]	tyrosine metabolism	42.86	6 of 14
[Ref.: #66794]	ubiquinone biosynthesis	42.86	3 of 7
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
[Ref.: #66794]	gallate degradation	40	2 of 5
[Ref.: #66794]	glycine metabolism	40	4 of 10
[Ref.: #66794]	bacilysin biosynthesis	40	2 of 5
[Ref.: #66794]	3-chlorocatechol degradation	40	2 of 5
[Ref.: #66794]	O-antigen biosynthesis	40	2 of 5
[Ref.: #66794]	metabolism of amino sugars and derivatives	40	2 of 5
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	vitamin B6 metabolism	36.36	4 of 11
[Ref.: #66794]	d-xylose degradation	36.36	4 of 11
[Ref.: #66794]	sphingosine metabolism	33.33	2 of 6
[Ref.: #66794]	methane metabolism	33.33	1 of 3
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	octane oxidation	33.33	1 of 3
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	cyanate degradation	33.33	1 of 3
[Ref.: #66794]	arachidonic acid metabolism	33.33	6 of 18
[Ref.: #66794]	lipid A biosynthesis	33.33	3 of 9
[Ref.: #66794]	selenocysteine biosynthesis	33.33	2 of 6
[Ref.: #66794]	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	30.77	4 of 13
[Ref.: #66794]	myo-inositol biosynthesis	30	3 of 10
[Ref.: #66794]	ascorbate metabolism	27.27	6 of 22
[Ref.: #66794]	butanoate fermentation	25	1 of 4
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	CMP-KDO biosynthesis	25	1 of 4
[Ref.: #66794]	carnitine metabolism	25	2 of 8
[Ref.: #66794]	vitamin E metabolism	25	1 of 4
[Ref.: #66794]	biotin biosynthesis	25	1 of 4
[Ref.: #66794]	nitrate assimilation	22.22	2 of 9
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #65468]	Sample type/isolated from	an acidic thermal spring
[Ref.: #65468]	Geographic location (country and/or sea, region)	Los Nevados Natural National Park
[Ref.: #65468]	Country	Colombia
[Ref.: #65468]	Country ISO 3 Code	COL
[Ref.: #65468]	Continent	Middle and South America
[Ref.: #65468]	Geographic location	4.9703°/-75.3783°
[Ref.: #65468]	Enrichment culture duration	3 weeks
[Ref.: #65468]	Enrichment culture temperature	55 ̊C
[Ref.: #65468]	Isolation/enrichment procedure	samples were enriched by adding 0.1 % (v/v) commercial olive oil
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Thermal spring
#Condition	#Acidic	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KU366611 (>99% sequence identity) for Alicyclobacillus from Microbeatlas

Aquatic Samples	91
Soil Samples	33
Animal Samples	10
Plant Samples
Total Samples	134

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65468]	16S rRNA gene sequence	KU366611		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Alicyclobacillus montanus USBA-GBX-503	GCA_900142255	scaffold	GenBank	1830138 tax ID	*

[Ref.: #65468]

GC-content

44.6 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Alicyclobacillus montanus USBA-GBX-503 NCBI: GCA_900142255.1
[Ref.: #69481]	spore-forming	no	89	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Alicyclobacillus montanus USBA-GBX-503 NCBI: GCA_900142255.1
[Ref.: #69480]	motile	yes	81.812	yes
[Ref.: #69480]	flagellated	no	81.85	no
[Ref.: #69480]	gram-positive	yes	88.473	yes
[Ref.: #69480]	anaerobic	no	96.053	no
[Ref.: #69480]	aerobic	yes	90.49	no
[Ref.: #69480]	halophile	no	93.567	no
[Ref.: #69480]	spore-forming	yes	95.322	yes
[Ref.: #69480]	thermophile	yes	99.459	no
[Ref.: #69480]	glucose-util	yes	89.436	no
[Ref.: #69480]	glucose-ferment	no	85.985	no

Availability in culture collections External links

[Ref.: #65468]

Culture collection no.

CMPUJ UGB U503, CBMAI 1927

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Description of Alicyclobacillus montanus sp. nov., a mixotrophic bacterium isolated from acidic hot springs.	Lopez G, Diaz-Cardenas C, David Alzate J, Gonzalez LN, Shapiro N, Woyke T, Kyrpides NC, Restrepo S, Baena S	Int J Syst Evol Microbiol	10.1099/ijsem.0.002718	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#65468

G. López, C. Díaz-Cárdenas, J. David Alzate, L. N. Gonzalez, N. Shapiro, T. Woyke, N. C. Kyrpides, S. Restrepo, S. Baena: Description of Alicyclobacillus montanus sp. nov., a mixotrophic bacterium isolated from acidic hot springs. IJSEM 68: 1608 - 1615 2018 ( DOI 10.1099/ijsem.0.002718 , PubMed 29557767 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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