Name: Ancylobacter pratisalsi E130
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 158256

Type strain:

Species: Ancylobacter pratisalsi

Strain Designation: E130

Culture col. no.: DSM 102029, LMG 29367

Strain history: <- C. Suarez, Justus-Liebig-Universität, Giessen, Germany; E130 <- S. Ratering

NCBI tax ID(s): 1745854 (species)

SI-ID 403424

DSM 102029

Section

Ancylobacter pratisalsi E130 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from rhizosphere of a salt-meadow greater plantain .

colony-forming
Gram-negative
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Xanthobacteraceae*
	Genus	*Ancylobacter*
	Species	**Ancylobacter pratisalsi**
	Full Scientific Name (LPSN)	Ancylobacter pratisalsi Suarez et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #65259]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.988

[Ref.: #65259]	Cell length	1.3-1.7 µm

[Ref.: #65259]	Cell width	0.5-0.6 µm

[Ref.: #65259]	Cell shape	rod-shaped

[Ref.: #65259]	Motility	no

[Ref.: #65259]	Colony size	0.5-1 mm
[Ref.: #65259]	Colony color	white
[Ref.: #65259]	Colony shape	circular
[Ref.: #65259]	Incubation period	7 days
[Ref.: #65259]	Cultivation medium used	R2A agar

[Ref.: #65259]	Colony size	0.3-0.5 mm
[Ref.: #65259]	Colony color	non-pigmented
[Ref.: #65259]	Colony shape	circular
[Ref.: #65259]	Incubation period	7 days
[Ref.: #65259]	Cultivation medium used	LG agar

[Ref.: #65259]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #64915]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

LG agar

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Culture medium growth

	Kind of temperature		Temperature
[Ref.: #64915]		growth	28 °C
[Ref.: #65259]		growth	4-7 °C
[Ref.: #65259]	no	growth	45 °C

[Ref.: #64915]	Temperature range	mesophilic
[Ref.: #65259]	Temperature range	psychrophilic
[Ref.: #65259]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #65259]

Oxygen tolerance

aerobe

[Ref.: #65259]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.999
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #65259]		NaCl		growth	0.5-5	%(w/v)

[Ref.: #65259]

Observation

No growth occurrs under microaerophilic or anoxic conditions.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #65259]	acetate ChEBI	+	carbon source
[Ref.: #65259]	alanine ChEBI	+	carbon source
[Ref.: #65259]	arabinose ChEBI	-	assimilation
[Ref.: #65259]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #65259]	citrate ChEBI	-	assimilation
[Ref.: #65259]	D-glucose ChEBI	-	fermentation
[Ref.: #65259]	D-glucose ChEBI	+	assimilation
[Ref.: #65259]	decanoate ChEBI	-	assimilation
[Ref.: #65259]	esculin ChEBI	+	hydrolysis
[Ref.: #65259]	formate ChEBI	+	carbon source
[Ref.: #65259]	gelatin ChEBI	+	hydrolysis
[Ref.: #65259]	gluconate ChEBI	-	assimilation
[Ref.: #65259]	glucose ChEBI	+	carbon source
[Ref.: #65259]	lactose ChEBI	+	carbon source
[Ref.: #65259]	malate ChEBI	-	assimilation
[Ref.: #65259]	maltose ChEBI	-	assimilation
[Ref.: #65259]	mannitol ChEBI	+	assimilation
[Ref.: #65259]	mannitol ChEBI	+	carbon source
[Ref.: #65259]	mannose ChEBI	-	assimilation
[Ref.: #65259]	methanol ChEBI	+	carbon source
[Ref.: #65259]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #65259]	nitrate ChEBI	+	reduction
[Ref.: #65259]	phenylacetate ChEBI	-	assimilation
[Ref.: #65259]	proline ChEBI	+	carbon source
[Ref.: #65259]	propionate ChEBI	+	carbon source
[Ref.: #65259]	rhamnose ChEBI	+	carbon source
[Ref.: #65259]	ribose ChEBI	+	carbon source
[Ref.: #65259]	starch ChEBI	+	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #65259]	ampicillin ChEBI	yes	10	µg (disc)
[Ref.: #65259]	erythromycin ChEBI				yes	15	µg (disc)
[Ref.: #65259]	gentamicin ChEBI	yes	10	µg (disc)
[Ref.: #65259]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #65259]	penicillin g ChEBI				yes	10	µg (disc)
[Ref.: #65259]	tetracycline ChEBI	yes	30	µg (disc)

	Metabolite production	Metabolite	Production
[Ref.: #65259]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #65259]	acid phosphatase	+	3.1.3.2
[Ref.: #65259]	alkaline phosphatase	+	3.1.3.1
[Ref.: #65259]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #65259]	alpha-fucosidase	-	3.2.1.51
[Ref.: #65259]	alpha-galactosidase	-	3.2.1.22
[Ref.: #65259]	alpha-glucosidase	-	3.2.1.20
[Ref.: #65259]	alpha-mannosidase	-	3.2.1.24
[Ref.: #65259]	arginine dihydrolase	-	3.5.3.6
[Ref.: #65259]	beta-glucosidase	-	3.2.1.21
[Ref.: #65259]	beta-glucuronidase	-	3.2.1.31
[Ref.: #65259]	catalase	+	1.11.1.6
[Ref.: #65259]	cystine arylamidase	-	3.4.11.3
[Ref.: #65259]	cytochrome oxidase	+	1.9.3.1
[Ref.: #65259]	esterase (C 4)	+
[Ref.: #65259]	esterase Lipase (C 8)	-
[Ref.: #65259]	leucine arylamidase	+	3.4.11.1
[Ref.: #65259]	lipase (C 14)	-
[Ref.: #65259]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #65259]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #65259]	trypsin	+	3.4.21.4
[Ref.: #65259]	urease	-	3.5.1.5
[Ref.: #65259]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #65259]

Fatty acid	Percentage
C18:1ω7c	64.4
C16:0	12.7
C19:0ω8c cyclo	7.7
C12:0	6.6
C16:1ω7c and/or C15:0 Iso 2OH	3.6
C16:1 iso I and/or C14:0 iso 3OH	2.1
C18:0	1.6
C17:0 cyclo	1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #64915]	Sample type/isolated from	rhizosphere of a salt-meadow greater plantain (Plantago winteri Wirtg.)
[Ref.: #64915]	Geographic location (country and/or sea, region)	Münzenberg, Hessen (50° 27' 36'' N, 8° 44' 35'' E)
[Ref.: #64915]	Country	Germany
[Ref.: #64915]	Country ISO 3 Code	DEU
[Ref.: #64915]	Continent	Europe
[Ref.: #64915]	Geographic location	50.4600°/8.7431°

[Ref.: #65259]	Sample type/isolated from	rhizosphere of Plantago winteri Wirtg.
[Ref.: #65259]	Geographic location (country and/or sea, region)	nature protection area nearby Münzenberg, Hessen
[Ref.: #65259]	Country	Germany
[Ref.: #65259]	Country ISO 3 Code	DEU
[Ref.: #65259]	Continent	Europe
[Ref.: #65259]	Geographic location	50.4600°/8.7430°
[Ref.: #65259]	Enrichment culture	nitrogen-free LG medium
[Ref.: #65259]	Enrichment culture duration	2-3 days
[Ref.: #65259]	Enrichment culture temperature	28 ̊C
[Ref.: #65259]	Isolation/enrichment procedure	Salt-meadow greater plantain (Plantago winteri) root systems were washed thoroughly by vigorous shaking in sodium pyrophosphate (100 mM). Subsequently, the suspensions were diluted with sterile saline using standard dilution technique and then incubated at 28°C for 2 to 3 days on nitrogen-free LG medium.
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Rhizosphere
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #64915]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #65259]	16S rRNA gene sequence	KX021302		GenBank
[Ref.: #64915]	Ancylobacter pratisalsi strain E130 16S ribosomal RNA gene, partial sequence	KR703488	1324	ENA	1745854 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Ancylobacter pratisalsi DSM 102029	GCA_010669125	complete	GenBank	1745854 tax ID	*
[Ref.: #66792]	Ancylobacter pratisalsi strain DSM 102029	1745854.3	complete	PATRIC	1745854 tax ID	*
[Ref.: #66792]	Ancylobacter pratisalsi DSM 102029	2883754999	complete	JGI IMG/M	1745854 tax ID	*

[Ref.: #64915]

GC-content

65.1 mol%

fluorimetric

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Ancylobacter pratisalsi DSM 102029 NCBI: GCA_010669125.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Ancylobacter pratisalsi DSM 102029 NCBI: GCA_010669125.1
[Ref.: #69480]	motile	no	85.31	no
[Ref.: #69480]	flagellated	no	93.634	yes
[Ref.: #69480]	gram-positive	no	98.233	yes
[Ref.: #69480]	anaerobic	no	97.665	yes
[Ref.: #69480]	aerobic	yes	91.366	no
[Ref.: #69480]	halophile	no	80.921	no
[Ref.: #69480]	spore-forming	no	96.513	yes
[Ref.: #69480]	thermophile	no	96.849	no
[Ref.: #69480]	glucose-util	yes	92.804	no
[Ref.: #69480]	glucose-ferment	no	88.312	yes

Availability in culture collections External links

[Ref.: #64915]

Culture collection no.

DSM 102029, LMG 29367

[Ref.: #111075]

SI-ID 403424

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Ancylobacter pratisalsi sp. nov. with plant growth promotion abilities from the rhizosphere of Plantago winteri Wirtg.	Suarez C, Ratering S, Schafer J, Schnell S	Int J Syst Evol Microbiol	10.1099/ijsem.0.002320	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#64915

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 102029

#65259

Christian Suarez, Stefan Ratering, Johanna Schäfer, Sylvia Schnell: Ancylobacter pratisalsi sp. nov. with plant growth promotion abilities from the rhizosphere of Plantago winteri Wirtg. IJSEM 67: 4500 - 4506 2017 ( DOI 10.1099/ijsem.0.002320 , PubMed 28945527 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#111075

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID403424.1 )

* These data were automatically processed and therefore are not curated

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Ancylobacter pratisalsi DSM 102029

Ancylobacter pratisalsi DSM 102029

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