Name: Balneicella halophila KHALHBd91
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 133009

Type strain:

Species: Balneicella halophila

Strain Designation: KHALHBd91

Culture col. no.: DSM 28579, JCM 19909

Strain history: M.-L. Fardeau; IRD-MIO, Aix-Marseille Univ., France; KHALBd91.

NCBI tax ID(s): 1537566 (species)

SI-ID 405971

DSM 28579

Section

Balneicella halophila KHALHBd91 is an anaerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from water from a mesothermic spring.

colony-forming
Gram-negative
rod-shaped
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Bacteroidia*
	Order	*Bacteroidales*
	Family	*Balneicellaceae*
	Genus	*Balneicella*
	Species	**Balneicella halophila**
	Full Scientific Name (LPSN)	Balneicella halophila Fadhlaoui et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #24999]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.999

[Ref.: #24999]	Cell length	0.5-2.0 µm

[Ref.: #24999]	Cell width	0.5-1.0 µm

[Ref.: #24999]	Cell shape	rod-shaped

[Ref.: #24999]	Motility	no

[Ref.: #24999]	Colony size	0.5-1.0 mm
[Ref.: #24999]	Colony color	yellow
[Ref.: #24999]	Colony shape	circular
[Ref.: #24999]	Incubation period	2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24797]

Culture medium

MARINIFILUM MEDIUM (IRD MEDIUM) (DSMZ Medium 1533)

[Ref.: #24797]

Culture medium growth

[Ref.: #24797]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24797]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #24797]	growth	35 °C
[Ref.: #24999]	optimum	37 °C
[Ref.: #24999]	growth	20.0-50.0 °C
[Ref.: #67770]	growth	35 °C

[Ref.: #24797]	Temperature range	mesophilic
[Ref.: #24999]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #24999]			optimum	7
[Ref.: #24999]			growth	5.5-7.8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #24999]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %92.694

[Ref.: #24999]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.983

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #24999]		NaCl		growth	0.5-8.5	%
[Ref.: #24999]		NaCl		optimum	2.5	%

[Ref.: #67770]

Observation

quinones: MK-6

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #24999]	casamino acids	-	electron donor
[Ref.: #24999]	elemental sulfur ChEBI	-	electron acceptor
[Ref.: #24999]	fructose ChEBI	-	electron donor
[Ref.: #24999]	fumarate ChEBI	+	electron donor
[Ref.: #24999]	galactose ChEBI	+	electron donor
[Ref.: #24999]	glucose ChEBI	+	electron donor
[Ref.: #24999]	glucose ChEBI	+	fermentation
[Ref.: #24999]	glycerol ChEBI	-	electron donor
[Ref.: #24999]	lactate ChEBI	+	electron donor
[Ref.: #24999]	lactose ChEBI	-	electron donor
[Ref.: #24999]	maltose ChEBI	+	electron donor
[Ref.: #24999]	mannitol ChEBI	-	electron donor
[Ref.: #24999]	mannose ChEBI	-	electron donor
[Ref.: #24999]	melibiose ChEBI	-	electron donor
[Ref.: #24999]	methanol ChEBI	-	electron donor
[Ref.: #24999]	pyruvate ChEBI	+	electron donor
[Ref.: #24999]	sodium nitrate ChEBI	-	electron acceptor
[Ref.: #24999]	sodium nitrite ChEBI	-	electron acceptor
[Ref.: #24999]	sodium sulfate ChEBI	-	electron acceptor
[Ref.: #24999]	sodium sulfite ChEBI	-	electron acceptor
[Ref.: #24999]	sodium thiosulfate ChEBI	-	electron acceptor
[Ref.: #24999]	starch ChEBI	-	electron donor
[Ref.: #24999]	succinate ChEBI	-	electron donor
[Ref.: #24999]	xylose ChEBI	-	electron donor
[Ref.: #24999]	yeast extract	+	electron donor
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24797]	Sample type/isolated from	water from a mesothermic (45°C) spring
[Ref.: #24797]	Geographic location (country and/or sea, region)	Siliana, region of El Krib, Hammam Biadha (35.77524° N 9.26674° E)
[Ref.: #24797]	Country	Tunisia
[Ref.: #24797]	Country ISO 3 Code	TUN
[Ref.: #24797]	Continent	Africa
[Ref.: #24797]	Geographic location	35.7752°/9.2668°

[Ref.: #67770]	Sample type/isolated from	Moderately hot spring of Hammam Biadha
[Ref.: #67770]	Country	Tunisia
[Ref.: #67770]	Country ISO 3 Code	TUN
[Ref.: #67770]	Continent	Africa

[Ref.: #24999]	Enrichment culture	basal culture medium (BM)
[Ref.: #24999]	Enrichment culture composition	containing (g l-1): NH4Cl (1), K2HPO4 (0.3), KH2PO4 (0.3), KCl (0.1), NaCl (20), Yeast extract (1), CaCl2 , 2H2O (0.1), Cysteine-HCl (0.5), Widdel trace element solution (1 ml) (Widdel & Pfennig, 1981) and 1 ml 0.1 % resazurin
[Ref.: #24999]	Enrichment culture temperature	37 ̊C
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Thermal spring

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24797]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24797]	Bacteroides sp. KhalHBD91 partial 16S rRNA gene, strain KhalHBD91	LM999900	1505	ENA	1537566 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Balneicella halophila DSM 28579	GCA_003096835	scaffold	GenBank	1537566 tax ID	*
[Ref.: #66792]	Balneicella halophila strain DSM 28579	1537566.3	wgs	PATRIC	1537566 tax ID	*
[Ref.: #66792]	Balneicella halophila DSM 28579	2756170310	draft	JGI IMG/M	1537566 tax ID	*

[Ref.: #24999]	GC-content	35 mol%	high performance liquid chromatography (HPLC)
[Ref.: #24797]	GC-content	35.0 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Balneicella halophila DSM 28579 NCBI: GCA_003096835.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Balneicella halophila DSM 28579 NCBI: GCA_003096835.1
[Ref.: #69480]	flagellated	no	97.801	yes
[Ref.: #69480]	gram-positive	no	97.879	yes
[Ref.: #69480]	anaerobic	yes	76.935	no
[Ref.: #69480]	halophile	yes	55.91	no
[Ref.: #69480]	spore-forming	no	96.804	yes
[Ref.: #69480]	glucose-util	yes	83.377	no
[Ref.: #69480]	aerobic	no	95.202	yes
[Ref.: #69480]	thermophile	no	98.513	yes
[Ref.: #69480]	motile	no	91.506	yes
[Ref.: #69480]	glucose-ferment	no	57.673	no

Availability in culture collections External links

[Ref.: #24797]

Culture collection no.

DSM 28579, JCM 19909

[Ref.: #91283]

SI-ID 405971

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Balneicella halophila gen. nov., sp. nov., an anaerobic bacterium, isolated from a thermal spring and description of Balneicellaceae fam. nov.	Fadhlaoui K, Ben Hania W, Postec A, Hamdi M, Ollivier B, Fardeau ML	Int J Syst Evol Microbiol	10.1099/ijsem.0.001412	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24797

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28579

#24999

K. B. H. Fadhlaoui, Wajdi,Postec, Anne,Hamdi, Moktar,Ollivier, Bernard,Fardeau, Marie-Laure: Balneicella halophila gen. nov., sp. nov., an anaerobic bacterium, isolated from a thermal spring and description of Balneicellaceae fam. nov. IJSEM 66: 4692 - 4696 2016 ( DOI 10.1099/ijsem.0.001412 , PubMed 27604340 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#91283

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405971.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Balneicella halophila in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Balneicella halophila DSM 28579

Balneicella halophila DSM 28579

Change proposal

Change proposal