Name: Legionella gratiana 8420412
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6759

Type strain:

Species: Legionella gratiana

Strain Designation: 8420412

Culture col. no.: DSM 21233, ATCC 49413, CCUG 44894, CIP 105267, NCTC 12388, Lyon 8420412

Strain history: CIP <- 1997, CDC <- N. Bornstein, Lyon, France: strain 8420412

NCBI tax ID(s): 45066 (species)

SI-ID 93040

ATCC 49413, ATCC 49668, CIP 105267, CCUG 44894, CIP 105267.8420412, NCTC 12388, DSM 21233

Section

Legionella gratiana 8420412 is a microaerophile, mesophilic, Gram-negative human pathogen that was isolated from thermal spa.

Gram-negative
rod-shaped
human pathogen
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Legionellales*
	Family	*Legionellaceae*
	Genus	*Legionella*
	Species	**Legionella gratiana**
	Full Scientific Name (LPSN)	Legionella gratiana Bornstein et al. 1991

Information on morphological and physiological properties Morphology

[Ref.: #119732]	Gram stain	negative

[Ref.: #119732]	Cell shape	rod-shaped

[Ref.: #119732]	Motility	no

[Ref.: #15586]

Incubation period

2-3 days

Information on culture and growth conditions Culture and growth conditions

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Culture medium

BCYE-AGAR (DSMZ Medium 585)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #15586]

Culture medium composition

expand / minimize

[Ref.: #39243]

Culture medium

MEDIUM 23 - for Afipia and Legionella

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Culture medium growth

[Ref.: #39243]

Culture medium composition

expand / minimize

[Ref.: #119732]

Culture medium

CIP Medium 23

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #15586]	growth	37 °C
[Ref.: #39243]	growth	37 °C
[Ref.: #56532]	growth	37 °C

[Ref.: #15586]	Temperature range	mesophilic
[Ref.: #39243]	Temperature range	mesophilic
[Ref.: #56532]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #15586]	Oxygen tolerance	microaerophile
[Ref.: #56532]	Oxygen tolerance	microaerophile

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #15586]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #15586]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #56532]

Fatty acid	Percentage	ECL
C16:1 ω7c	24.9	15.819
C16:0 iso	23.6	15.626
C15:0 ANTEISO	12.7	14.711
C14:0 ISO	11.4	13.618
C16:0	6.7	16
C17:0 CYCLO	6.5	16.888
C15:1 ω6c	4.8	14.856
C15:0	2.6	15
C17:0 anteiso	1.7	16.722
C14:1 ω5c	1.4	13.899
C16:1 ω5c	1.4	15.908
C14:0	0.9	14
C17:0	0.8	17
C15:0 ISO	0.5	14.621

Metadata FA analysis

Reference

[Ref.: #56532]

Fatty acid	Percentage	ECL
C16:1 ω7c	26.2	15.819
C16:0 iso	25.1	15.626
C14:0 ISO	12.1	13.618
C15:0 ANTEISO	11.9	14.711
C17:0 CYCLO	7.9	16.888
C16:0	4.8	16
C15:1 ω6c	4.4	14.856
C17:0 anteiso	1.4	16.722
C15:0	1.2	15
C16:1 ω5c	1	15.908
C14:1 ω5c	0.9	13.899
C16:0 iso 3OH	0.7	17.145
C14:0	0.6	14
C15:0 ISO	0.6	14.621
C17:0	0.6	17
C16:1 ISO H	0.5	15.461

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119732]	8571	-	+	+	+	-	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15586]	Sample type/isolated from	thermal spa
[Ref.: #15586]	Country	France
[Ref.: #15586]	Country ISO 3 Code	FRA
[Ref.: #15586]	Continent	Europe

[Ref.: #56532]	Sample type/isolated from	Thermal spa water
[Ref.: #56532]	Geographic location (country and/or sea, region)	Savoie
[Ref.: #56532]	Country	France
[Ref.: #56532]	Country ISO 3 Code	FRA
[Ref.: #56532]	Continent	Europe

[Ref.: #119732]	Sample type/isolated from	Environment, Thermal spa water
[Ref.: #119732]	Geographic location (country and/or sea, region)	Savoie
[Ref.: #119732]	Country	France
[Ref.: #119732]	Country ISO 3 Code	FRA
[Ref.: #119732]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15586]	Pathogenicity (human)	yes, in single cases
[Ref.: #15586]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119732]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15586]	L.gratiana gene for ribosomal RNA, small subunit	Z49725	1400	ENA	45066 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Legionella gratiana NCTC12388	GCA_900452545	contig	GenBank	45066 tax ID	*
[Ref.: #66792]	Legionella gratiana strain Lyon 8420412	45066.5	wgs	PATRIC	45066 tax ID	*
[Ref.: #66792]	Legionella gratiana strain NCTC12388	45066.7	wgs	PATRIC	45066 tax ID	*
[Ref.: #66792]	Legionella gratiana NCTC 12388	2925233983	draft	JGI IMG/M	45066 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Legionella gratiana strain Lyon 8420412 PATRIC: 45066.5
[Ref.: #69480]	gram-positive	no	98.359	no
[Ref.: #69480]	anaerobic	no	98.489	no
[Ref.: #69480]	halophile	no	90.509	no
[Ref.: #69480]	spore-forming	no	92.986	no
[Ref.: #69480]	glucose-util	yes	71.293	no
[Ref.: #69480]	aerobic	no	90.852	yes
[Ref.: #69480]	flagellated	no	95.478	no
[Ref.: #69480]	motile	no	72.942	no
[Ref.: #69480]	thermophile	no	98.95	yes
[Ref.: #69480]	glucose-ferment	no	88.976	no

Availability in culture collections External links

[Ref.: #15586]

Culture collection no.

DSM 21233, ATCC 49413, CCUG 44894, CIP 105267, NCTC 12388, Lyon 8420412

[Ref.: #76229]

SI-ID 93040

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Legionella gratiana sp. nov. isolated from French spa water.	Bornstein N, Marmet D, Surgot M, Nowicki M, Meugnier H, Fleurette J, Ageron E, Grimont F, Grimont PA, Thacker WL, et al.	Res Microbiol	10.1016/0923-2508(89)90086-7	1989	*

References

#15586

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21233

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#39243 ; Curators of the CIP;
#56532 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 44894

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76229

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID93040.1 )

#119732 Collection of Institut Pasteur ; Curators of the CIP; CIP 105267
* These data were automatically processed and therefore are not curated

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Legionella gratiana strain Lyon 8420412

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