Name: Piscinibacter sakaiensis 201-F6
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140803

Type strain:

Species: Piscinibacter sakaiensis

Strain Designation: 201-F6

Culture col. no.: NBRC 110686, TISTR 2288, DSM 112585

Strain history: <- NBRC; NBRC 110686 <- K. Oda, Graduate School Sci. Technol., Kyoto; 201-F6

NCBI tax ID(s): 1547922 (species)

SI-ID 402444

DSM 112585

Section

Piscinibacter sakaiensis 201-F6 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from a microbial consortium that degrades poly .

colony-forming
Gram-negative
motile
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	[Burkholderiales, not assigned to family]
	Genus	*Piscinibacter*
	Species	**Piscinibacter sakaiensis**
	Full Scientific Name (LPSN)	Piscinibacter sakaiensis (Tanasupawat et al. 2016) Liu et al. 2023

Synonym

Ideonella sakaiensis

Information on morphological and physiological properties Morphology

[Ref.: #43583]	Gram stain	negative

[Ref.: #43583]	Cell length	1.2-1.5 µm

[Ref.: #43583]	Cell width	0.6-0.8 µm

[Ref.: #43583]	Cell shape	rod-shaped

[Ref.: #43583]	Motility	yes

[Ref.: #43583]	Flagellum arrangement	monotrichous

[Ref.: #43583]	Colony size	0.5-1 mm
[Ref.: #43583]	Colony color	Translucent to cream
[Ref.: #43583]	Colony shape	circular
[Ref.: #43583]	Incubation period	2 days
[Ref.: #43583]	Cultivation medium used	NBRC no. 802 agar medium

Information on culture and growth conditions Culture and growth conditions

[Ref.: #67666]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #67666]

Culture medium growth

[Ref.: #67666]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #43583]

Culture medium

NBRC no. 802 agar medium

[Ref.: #43583]

Culture medium growth

[Ref.: #67666]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #67666]

Culture medium growth

[Ref.: #67666]

Culture medium link

Medium recipe at MediaDive

[Ref.: #67666]

Culture medium composition

expand / minimize

[Ref.: #67666]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #67666]

Culture medium growth

[Ref.: #67666]

Culture medium link

Medium recipe at MediaDive

[Ref.: #67666]

Culture medium composition

expand / minimize

[Ref.: #67666]

Culture medium

TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)

[Ref.: #67666]

Culture medium growth

[Ref.: #67666]

Culture medium link

Medium recipe at MediaDive

[Ref.: #67666]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #43583]	growth	15-42 °C
[Ref.: #43583]	optimum	30-37 °C
[Ref.: #67666]	growth	28 °C
[Ref.: #67666]	growth	35 °C

[Ref.: #43583]	Temperature range	mesophilic
[Ref.: #67666]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43583]			optimum	7-7.5
[Ref.: #43583]			growth	5.5-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43583]

Oxygen tolerance

aerobe

[Ref.: #43583]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43583]		NaCl	no	growth	3	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43583]	adipate ChEBI	+	assimilation
[Ref.: #43583]	arginine ChEBI	-	hydrolysis
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #43583]	citrate ChEBI	+/-	assimilation
[Ref.: #43583]	D-glucose ChEBI	-	assimilation
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #43583]	D-mannitol ChEBI	-	assimilation
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #43583]	D-mannose ChEBI	-	assimilation
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #43583]	decanoate ChEBI	-	assimilation
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #43583]	esculin ChEBI	-	hydrolysis
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #43583]	gelatin ChEBI	-	hydrolysis
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #43583]	glucose ChEBI	-	fermentation
[Ref.: #43583]	L-arabinose ChEBI	-	assimilation
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #43583]	malate ChEBI	+/-	assimilation
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #43583]	maltose ChEBI	+	assimilation
[Ref.: #43583]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #43583]	nitrate ChEBI	-	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #43583]	phenylacetate ChEBI	-	assimilation
[Ref.: #43583]	poly(ethylene terephthalate) ChEBI	+	degradation
[Ref.: #43583]	potassium gluconate ChEBI	+	assimilation
[Ref.: #43583]	sodium citrate ChEBI	+/-	assimilation
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #43583]	urea ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43583]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #43583]	acid phosphatase	+/-	3.1.3.2
[Ref.: #43583]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43583]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #43583]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43583]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43583]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43583]	alpha-mannosidase	-	3.2.1.24
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #43583]	beta-galactosidase	-	3.2.1.23
[Ref.: #43583]	beta-glucosidase	-	3.2.1.21
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #43583]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43583]	catalase	+	1.11.1.6
[Ref.: #43583]	cystine arylamidase	-	3.4.11.3
[Ref.: #43583]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #43583]	esterase (C 4)	-
[Ref.: #43583]	esterase Lipase (C 8)	+
[Ref.: #43583]	leucine arylamidase	+	3.4.11.1
[Ref.: #43583]	lipase (C 14)	-
[Ref.: #43583]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43583]	naphthol-AS-BI-phosphohydrolase	+/-
[Ref.: #43583]	trypsin	-	3.4.21.4
[Ref.: #43583]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #67666]	25254	-	-	-	-	+	-	+	-	+	-	-	-	-	+	+	-	-	-	-	-	+
[Ref.: #67666]	25202	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	+/-	-	-	-	+
[Ref.: #67666]	25337	-	-	-	-	+	-	+	-	-	-	-	-	-	+	+	-	-	-	-	-	+
[Ref.: #67666]	25474	-	-	-	-	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	+

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	threonine metabolism	100	10 of 10
[Ref.: #66794]	cyanate degradation	100	3 of 3
[Ref.: #66794]	CO2 fixation in Crenarchaeota	100	9 of 9
[Ref.: #66794]	phenylacetate degradation (aerobic)	100	5 of 5
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	glycogen metabolism	100	5 of 5
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	methylglyoxal degradation	100	5 of 5
[Ref.: #66794]	palmitate biosynthesis	100	22 of 22
[Ref.: #66794]	ethanol fermentation	100	2 of 2
[Ref.: #66794]	photosynthesis	100	14 of 14
[Ref.: #66794]	methanofuran biosynthesis	100	5 of 5
[Ref.: #66794]	molybdenum cofactor biosynthesis	100	9 of 9
[Ref.: #66794]	resorcinol degradation	100	2 of 2
[Ref.: #66794]	4-hydroxymandelate degradation	100	9 of 9
[Ref.: #66794]	reductive acetyl coenzyme A pathway	100	7 of 7
[Ref.: #66794]	lactate fermentation	100	4 of 4
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	taurine degradation	100	1 of 1
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	valine metabolism	100	9 of 9
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	butanoate fermentation	100	4 of 4
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	formaldehyde oxidation	100	3 of 3
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	Entner Doudoroff pathway	90	9 of 10
[Ref.: #66794]	propionate fermentation	90	9 of 10
[Ref.: #66794]	serine metabolism	88.89	8 of 9
[Ref.: #66794]	lipid A biosynthesis	88.89	8 of 9
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	d-mannose degradation	88.89	8 of 9
[Ref.: #66794]	gluconeogenesis	87.5	7 of 8
[Ref.: #66794]	isoleucine metabolism	87.5	7 of 8
[Ref.: #66794]	heme metabolism	85.71	12 of 14
[Ref.: #66794]	ubiquinone biosynthesis	85.71	6 of 7
[Ref.: #66794]	citric acid cycle	85.71	12 of 14
[Ref.: #66794]	leucine metabolism	84.62	11 of 13
[Ref.: #66794]	phenylalanine metabolism	84.62	11 of 13
[Ref.: #66794]	NAD metabolism	83.33	15 of 18
[Ref.: #66794]	glycolate and glyoxylate degradation	83.33	5 of 6
[Ref.: #66794]	glutamate and glutamine metabolism	82.14	23 of 28
[Ref.: #66794]	3-chlorocatechol degradation	80	4 of 5
[Ref.: #66794]	starch degradation	80	8 of 10
[Ref.: #66794]	hydrogen production	80	4 of 5
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	ethylmalonyl-CoA pathway	80	4 of 5
[Ref.: #66794]	gallate degradation	80	4 of 5
[Ref.: #66794]	purine metabolism	79.79	75 of 94
[Ref.: #66794]	histidine metabolism	79.31	23 of 29
[Ref.: #66794]	tetrahydrofolate metabolism	78.57	11 of 14
[Ref.: #66794]	allantoin degradation	77.78	7 of 9
[Ref.: #66794]	chlorophyll metabolism	77.78	14 of 18
[Ref.: #66794]	cysteine metabolism	77.78	14 of 18
[Ref.: #66794]	aspartate and asparagine metabolism	77.78	7 of 9
[Ref.: #66794]	methionine metabolism	76.92	20 of 26
[Ref.: #66794]	glycolysis	76.47	13 of 17
[Ref.: #66794]	alanine metabolism	75.86	22 of 29
[Ref.: #66794]	C4 and CAM-carbon fixation	75	6 of 8
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	sulfopterin metabolism	75	3 of 4
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	CMP-KDO biosynthesis	75	3 of 4
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	75	9 of 12
[Ref.: #66794]	flavin biosynthesis	73.33	11 of 15
[Ref.: #66794]	metabolism of disaccharids	72.73	8 of 11
[Ref.: #66794]	pentose phosphate pathway	72.73	8 of 11
[Ref.: #66794]	proline metabolism	72.73	8 of 11
[Ref.: #66794]	vitamin B6 metabolism	72.73	8 of 11
[Ref.: #66794]	cardiolipin biosynthesis	71.43	5 of 7
[Ref.: #66794]	tyrosine metabolism	71.43	10 of 14
[Ref.: #66794]	propanol degradation	71.43	5 of 7
[Ref.: #66794]	glutathione metabolism	71.43	10 of 14
[Ref.: #66794]	pyrimidine metabolism	71.11	32 of 45
[Ref.: #66794]	tryptophan metabolism	71.05	27 of 38
[Ref.: #66794]	lipid metabolism	70.97	22 of 31
[Ref.: #66794]	arginine metabolism	70.83	17 of 24
[Ref.: #66794]	4-hydroxyphenylacetate degradation	70	7 of 10
[Ref.: #66794]	vitamin B1 metabolism	69.23	9 of 13
[Ref.: #66794]	sulfate reduction	69.23	9 of 13
[Ref.: #66794]	urea cycle	69.23	9 of 13
[Ref.: #66794]	degradation of sugar alcohols	68.75	11 of 16
[Ref.: #66794]	oxidative phosphorylation	67.03	61 of 91
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	selenocysteine biosynthesis	66.67	4 of 6
[Ref.: #66794]	acetyl CoA biosynthesis	66.67	2 of 3
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	isoprenoid biosynthesis	65.38	17 of 26
[Ref.: #66794]	non-pathway related	63.16	24 of 38
[Ref.: #66794]	ketogluconate metabolism	62.5	5 of 8
[Ref.: #66794]	carnitine metabolism	62.5	5 of 8
[Ref.: #66794]	lipoate biosynthesis	60	3 of 5
[Ref.: #66794]	phenol degradation	60	12 of 20
[Ref.: #66794]	vitamin K metabolism	60	3 of 5
[Ref.: #66794]	elloramycin biosynthesis	60	3 of 5
[Ref.: #66794]	glycine betaine biosynthesis	60	3 of 5
[Ref.: #66794]	methanogenesis from CO2	58.33	7 of 12
[Ref.: #66794]	lysine metabolism	57.14	24 of 42
[Ref.: #66794]	nitrate assimilation	55.56	5 of 9
[Ref.: #66794]	3-phenylpropionate degradation	53.33	8 of 15
[Ref.: #66794]	degradation of sugar acids	52	13 of 25
[Ref.: #66794]	aminopropanol phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	pantothenate biosynthesis	50	3 of 6
[Ref.: #66794]	ribulose monophosphate pathway	50	1 of 2
[Ref.: #66794]	cyclohexanol degradation	50	2 of 4
[Ref.: #66794]	toluene degradation	50	2 of 4
[Ref.: #66794]	quinate degradation	50	1 of 2
[Ref.: #66794]	phenylmercury acetate degradation	50	1 of 2
[Ref.: #66794]	dTDPLrhamnose biosynthesis	50	4 of 8
[Ref.: #66794]	glycine metabolism	50	5 of 10
[Ref.: #66794]	degradation of pentoses	46.43	13 of 28
[Ref.: #66794]	degradation of hexoses	44.44	8 of 18
[Ref.: #66794]	androgen and estrogen metabolism	43.75	7 of 16
[Ref.: #66794]	polyamine pathway	43.48	10 of 23
[Ref.: #66794]	cellulose degradation	40	2 of 5
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	coenzyme M biosynthesis	40	4 of 10
[Ref.: #66794]	ascorbate metabolism	36.36	8 of 22
[Ref.: #66794]	cholesterol biosynthesis	36.36	4 of 11
[Ref.: #66794]	enterobactin biosynthesis	33.33	1 of 3
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
[Ref.: #66794]	sphingosine metabolism	33.33	2 of 6
[Ref.: #66794]	sulfoquinovose degradation	33.33	1 of 3
[Ref.: #66794]	vitamin B12 metabolism	32.35	11 of 34
[Ref.: #66794]	carotenoid biosynthesis	31.82	7 of 22
[Ref.: #66794]	bile acid biosynthesis, neutral pathway	29.41	5 of 17
[Ref.: #66794]	aclacinomycin biosynthesis	28.57	2 of 7
[Ref.: #66794]	benzoyl-CoA degradation	28.57	2 of 7
[Ref.: #66794]	arachidonic acid metabolism	27.78	5 of 18
[Ref.: #66794]	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
[Ref.: #66794]	catecholamine biosynthesis	25	1 of 4
[Ref.: #66794]	alginate biosynthesis	25	1 of 4
[Ref.: #66794]	biotin biosynthesis	25	1 of 4
[Ref.: #66794]	vitamin E metabolism	25	1 of 4
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	23.08	3 of 13
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43583]	Sample type/isolated from	a microbial consortium that degrades poly (ethylene terephthalate) (PET)
[Ref.: #43583]	Geographic location (country and/or sea, region)	Sakai City
[Ref.: #43583]	Country	Japan
[Ref.: #43583]	Country ISO 3 Code	JPN
[Ref.: #43583]	Continent	Asia

[Ref.: #67666]	Sample type/isolated from	environmental sediment samples contaminated with PET debris
[Ref.: #67666]	Geographic location (country and/or sea, region)	Sakai city
[Ref.: #67666]	Country	Japan
[Ref.: #67666]	Country ISO 3 Code	JPN
[Ref.: #67666]	Continent	Asia
[Ref.: #67666]	Geographic location	34.5700°/135.4130°
* marker position based on {}

Isolation sources categories

#Host

#Microbial

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC002525 (>99% sequence identity) for Ideonella sakaiensis from Microbeatlas

Aquatic Samples	72
Soil Samples	104
Animal Samples	37
Plant Samples	1
Total Samples	214

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #67666]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43583]	16S rRNA gene sequence	LC002525		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Ideonella sakaiensis 201-F6	GCA_001293525	contig	GenBank	1547922 tax ID	*

[Ref.: #43583]

GC-content

70.4 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Ideonella sakaiensis 201-F6 NCBI: GCA_001293525.1
[Ref.: #69480]	motile	yes	85.532	yes
[Ref.: #69480]	flagellated	yes	68.994	yes
[Ref.: #69480]	gram-positive	no	98.876	no
[Ref.: #69480]	anaerobic	no	96.979	no
[Ref.: #69480]	aerobic	yes	87.014	yes
[Ref.: #69480]	halophile	no	93.606	no
[Ref.: #69480]	spore-forming	no	94.243	no
[Ref.: #69480]	glucose-util	no	71.384	yes
[Ref.: #69480]	thermophile	no	97.346	yes
[Ref.: #69480]	glucose-ferment	no	91.114	yes

Availability in culture collections External links

[Ref.: #67666]

Culture collection no.

NBRC 110686, TISTR 2288, DSM 112585

[Ref.: #96794]

SI-ID 402444

Literature:

Only first 10 entries are displayed. Click here to see all.

	Title	Authors	Journal	DOI	Year
Enzymology	An Efficient Protein Evolution Workflow for the Improvement of Bacterial PET Hydrolyzing Enzymes.	Pirillo V, Orlando M, Tessaro D, Pollegioni L, Molla G	Int J Mol Sci	10.3390/ijms23010264	2021	*
Metabolism	Development of a Targeted Gene Disruption System in the Poly(Ethylene Terephthalate)-Degrading Bacterium Ideonella sakaiensis and Its Applications to PETase and MHETase Genes.	Hachisuka SI, Nishii T, Yoshida S	Appl Environ Microbiol	10.1128/AEM.00020-21	2021	*
Metabolism	QM/MM Study of the Enzymatic Biodegradation Mechanism of Polyethylene Terephthalate.	Boneta S, Arafet K, Moliner V	J Chem Inf Model	10.1021/acs.jcim.1c00394	2021	*
Metabolism	Assessment of the PETase conformational changes induced by poly(ethylene terephthalate) binding.	da Costa CHS, Dos Santos AM, Alves CN, Marti S, Moliner V, Santana K, Lameira J	Proteins	10.1002/prot.26155	2021	*
Metabolism	Emerging Roles of PETase and MHETase in the Biodegradation of Plastic Wastes.	Maity W, Maity S, Bera S, Roy A	Appl Biochem Biotechnol	10.1007/s12010-021-03562-4	2021	*
Metabolism	Protein engineering of stable IsPETase for PET plastic degradation by Premuse.	Meng X, Yang L, Liu H, Li Q, Xu G, Zhang Y, Guan F, Zhang Y, Zhang W, Wu N, Tian J	Int J Biol Macromol	10.1016/j.ijbiomac.2021.03.058	2021	*
Enzymology	Ideonella sakaiensis, PETase, and MHETase: From identification of microbial PET degradation to enzyme characterization.	Yoshida S, Hiraga K, Taniguchi I, Oda K	Methods Enzymol	10.1016/bs.mie.2020.12.007	2021	*
Metabolism	Enhanced Extracellular Production of IsPETase in Escherichia coli via Engineering of the pelB Signal Peptide.	Shi L, Liu H, Gao S, Weng Y, Zhu L	J Agric Food Chem	10.1021/acs.jafc.0c07469	2021	*
Metabolism	Class I hydrophobins pretreatment stimulates PETase for monomers recycling of waste PETs.	Puspitasari N, Tsai SL, Lee CK	Int J Biol Macromol	10.1016/j.ijbiomac.2021.02.026	2021	*
Enzymology	Novel Pet-Degrading Enzymes: Structure-Function from a Computational Perspective.	Berselli A, Ramos MJ, Menziani MC	Chembiochem	10.1002/cbic.202000841	2021	*
Enzymology	Functional expression of polyethylene terephthalate-degrading enzyme (PETase) in green microalgae.	Kim JW, Park SB, Tran QG, Cho DH, Choi DY, Lee YJ, Kim HS	Microb Cell Fact	10.1186/s12934-020-01355-8	2020	*
Metabolism	An engineered PET depolymerase to break down and recycle plastic bottles.	Tournier V, Topham CM, Gilles A, David B, Folgoas C, Moya-Leclair E, Kamionka E, Desrousseaux ML, Texier H, Gavalda S, Cot M, Guemard E, Dalibey M, Nomme J, Cioci G, Barbe S, Chateau M, Andre I, Duquesne S, Marty A	Nature	10.1038/s41586-020-2149-4	2020	*
Enzymology	Construction and Cloning of Plastic-degrading Recombinant Enzymes (MHETase).	Janatunaim RZ, Fibriani A	Recent Pat Biotechnol	10.2174/1872208314666200311104541	2020	*
Enzymology	In silico Screening and Heterologous Expression of a Polyethylene Terephthalate Hydrolase (PETase)-Like Enzyme (SM14est) With Polycaprolactone (PCL)-Degrading Activity, From the Marine Sponge-Derived Strain Streptomyces sp. SM14.	Almeida EL, Carrillo Rincon AF, Jackson SA, Dobson ADW	Front Microbiol	10.3389/fmicb.2019.02187	2019	*
Metabolism	Production of extracellular PETase from Ideonella sakaiensis using sec-dependent signal peptides in E. coli.	Seo H, Kim S, Son HF, Sagong HY, Joo S, Kim KJ	Biochem Biophys Res Commun	10.1016/j.bbrc.2018.11.087	2018	*
Biotechnology	Characterization and engineering of a plastic-degrading aromatic polyesterase.	Austin HP, Allen MD, Donohoe BS, Rorrer NA, Kearns FL, Silveira RL, Pollard BC, Dominick G, Duman R, El Omari K, Mykhaylyk V, Wagner A, Michener WE, Amore A, Skaf MS, Crowley MF, Thorne AW, Johnson CW, Woodcock HL, McGeehan JE, Beckham GT	Proc Natl Acad Sci U S A	10.1073/pnas.1718804115	2018	*
Metabolism	Comment on "A bacterium that degrades and assimilates poly(ethylene terephthalate)".	Yang Y, Yang J, Jiang L	Science	10.1126/science.aaf8305	2016	*
Phylogeny	Ideonella sakaiensis sp. nov., isolated from a microbial consortium that degrades poly(ethylene terephthalate).	Tanasupawat S, Takehana T, Yoshida S, Hiraga K, Oda K	Int J Syst Evol Microbiol	10.1099/ijsem.0.001058	2016	*
Metabolism	A bacterium that degrades and assimilates poly(ethylene terephthalate).	Yoshida S, Hiraga K, Takehana T, Taniguchi I, Yamaji H, Maeda Y, Toyohara K, Miyamoto K, Kimura Y, Oda K	Science	10.1126/science.aad6359	2016	*
Metabolism	Hydrophobic cell surface display system of PETase as a sustainable biocatalyst for PET degradation.	Jia Y, Samak NA, Hao X, Chen Z, Wen Q, Xing J	Front Microbiol	10.3389/fmicb.2022.1005480	2022	*
	Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis.	Fujiwara R, Sanuki R, Ajiro H, Fukui T, Yoshida S	Sci Rep	10.1038/s41598-021-99528-x	2021	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43583

Somboon Tanasupawat, Toshihiko Takehana, Shosuke Yoshida, Kazumi Hiraga, Kohei Oda: Ideonella sakaiensis sp. nov., isolated from a microbial consortium that degrades poly(ethylene terephthalate). IJSEM 66: 2813 - 2818 2016 ( DOI 10.1099/ijsem.0.001058 , PubMed 27045688 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#67666

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 112585

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#96794

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402444.1 )

* These data were automatically processed and therefore are not curated

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Ideonella sakaiensis 201-F6

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