Name: Lactococcus cremoris HP
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 14691

Type strain:

Species: Lactococcus cremoris

Strain Designation: HP

Culture col. no.: DSM 20069, ATCC 19257, NCDO 607, NCIB 8662, CCUG 21953, LMG 6897, IMET 10707, JCM 16167, BCRC 12586, CECT 7100, CIP 102301, CNCTC 6910, HAMBI 1588, NBRC 100676, NCFB 607, NCIMB 8662

Strain history: CIP <- 1986, NCFB, Streptococcus cremoris <- H.R. Whitehead: strain HP

NCBI tax ID(s): 1348655 (strain), 1359 (species)

SI-ID 92452

LMG 6897, ATCC 19257, CCUG 21953, DSM 20069, IMET 10707, NCFB 607, NCIMB 8662, NCDO 607, NCIB 8662, CIP 102301, CECT 697, CCRC 12586, KCTC 3619, NBRC 100676, BCRC 12586, HAMBI 1588, NCIM 2402, CECT 7100, CNCTC 6910, VTT E-96275, JCM 16167

Section

Lactococcus cremoris HP is a microaerophile, mesophilic, Gram-positive human pathogen of the family Streptococcaceae.

Gram-positive
rod-shaped
human pathogen
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Streptococcaceae*
	Genus	*Lactococcus*
	Species	**Lactococcus cremoris**
	Full Scientific Name (LPSN)	Lactococcus cremoris (Orla-Jensen 1919) Li et al. 2021

	Synonym	Streptococcus cremoris
	Synonym	Lactococcus lactis subsp. cremoris
	Synonym	Streptococcus lactis subsp. cremoris

Information on morphological and physiological properties Morphology

[Ref.: #118783]	Gram stain	positive

[Ref.: #118783]	Cell shape	rod-shaped

[Ref.: #118783]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8499]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #8499]

Culture medium growth

[Ref.: #8499]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8499]

Culture medium composition

expand / minimize

[Ref.: #42090]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #42090]

Culture medium growth

[Ref.: #42090]

Culture medium composition

expand / minimize

[Ref.: #118783]

Culture medium

CIP Medium 72

[Ref.: #118783]

Culture medium growth

[Ref.: #118783]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #118783]

Culture medium

CIP Medium 40

[Ref.: #118783]

Culture medium growth

[Ref.: #118783]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #8499]		growth	30 °C
[Ref.: #42090]		growth	30 °C
[Ref.: #47521]		growth	37 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #118783]		growth	15-30 °C
[Ref.: #118783]	no	growth	10 °C
[Ref.: #118783]	no	growth	37 °C
[Ref.: #118783]	no	growth	45 °C

[Ref.: #8499]	Temperature range	mesophilic
[Ref.: #42090]	Temperature range	mesophilic
[Ref.: #47521]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #118783]	Temperature range	psychrophilic
[Ref.: #118783]	Temperature range	mesophilic
[Ref.: #118783]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #47521]	Oxygen tolerance	microaerophile
[Ref.: #118783]	Oxygen tolerance	facultative anaerobe

[Ref.: #8499]	Murein short key	A11.31
[Ref.: #8499]	Murein types	A4alpha L-Lys-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68381]	alpha-cyclodextrin ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	arginine ChEBI	-	hydrolysis	* from API rID32STR
[Ref.: #68381]	D-arabitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	D-mannitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68381]	D-ribose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	D-tagatose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #68381]	glycogen ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68381]	L-arabinose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	lactose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68379]	lactose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68381]	maltose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	melezitose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	melibiose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	methyl beta-D-glucopyranoside ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #118783]	nitrate ChEBI	-	reduction
[Ref.: #118783]	nitrate ChEBI	+	respiration
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #118783]	nitrite ChEBI	-	reduction
[Ref.: #68381]	pullulan ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	raffinose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	sorbitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	sucrose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68381]	trehalose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne, API rID32STR
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #118783]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite production	Metabolite	Production
[Ref.: #68381]		acetoin ChEBI	yes	* from API rID32STR

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #68381]		acetoin ChEBI	+	* from API rID32STR

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68381]	Alanyl-Phenylalanyl-Proline arylamidase	+		* from API rID32STR
[Ref.: #118783]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68381]	alpha-galactosidase	-	3.2.1.22	* from API rID32STR, API zym
[Ref.: #68379]	alpha-glucosidase	-	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68381]	arginine dihydrolase	-	3.5.3.6	* from API rID32STR
[Ref.: #68381]	beta-galactosidase	-	3.2.1.23	* from API rID32STR, API Coryne, API zym
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68381]	beta-glucuronidase	-	3.2.1.31	* from API rID32STR, API Coryne, API zym
[Ref.: #68381]	beta-mannosidase	-	3.2.1.25	* from API rID32STR
[Ref.: #118783]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68381]	glycyl tryptophan arylamidase	-		* from API rID32STR
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #118783]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68381]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API rID32STR, API Coryne, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #118783]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #118783]	oxidase	-
[Ref.: #68379]	pyrazinamidase	+	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68381]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32STR
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68381]	urease	-	3.5.1.5	* from API rID32STR, API Coryne
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #47521]	855	-	+	+	-	-	-	-	-	+	-	-	-	not determinedn.d.	+	-	-	not determinedn.d.	+	-	-	not determinedn.d.

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #8499]	10664	-	+	+	-	-	-	-	-	-	+	-	-	-	-	-	-	+	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-
[Ref.: #8499]	10663	-	+	+/-	-	-	+	-	-	-	+	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #8499]	10662	-	+	+	-	-	+/-	-	-	-	+	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #8499]	10661	-	+/-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	+	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #118783]	7000	-	-	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #47521]	Sample type/isolated from	Cheese starter culture

[Ref.: #67770]	Sample type/isolated from	Raw milk

[Ref.: #42090]	Country	New zealand
[Ref.: #42090]	Country ISO 3 Code	NZL
[Ref.: #42090]	Continent	Australia and Oceania

[Ref.: #118783]	Country	New Zealand
[Ref.: #118783]	Country ISO 3 Code	NZL
[Ref.: #118783]	Continent	Australia and Oceania
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Dairy product
#Engineered	#Food production	#Starter culture

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC483568 (>99% sequence identity) for Lactococcus from Microbeatlas

Aquatic Samples	13841
Soil Samples	7135
Animal Samples	132033
Plant Samples	4677
Total Samples	157686

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8499]	Pathogenicity (human)	yes, in single cases
[Ref.: #8499]	Pathogenicity (animal)	yes, in single cases
[Ref.: #8499]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118783]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lactococcus lactis subsp. cremoris gene for 16S ribosomal RNA, partial sequence	LC483568	1485	ENA	1359 tax ID
[Ref.: #67770]	Lactococcus lactis subsp. cremoris gene for 16S rRNA, partial sequence, strain: YIT 2007 (= ATCC 19257)	AB008214	1540	ENA	1359 tax ID
[Ref.: #20218]	Lactococcus lactis subsp. lactis gene for 16S rRNA, partial sequence, strain: YIT 2008 (= ATCC 19435)	AB008215	1540	ENA	1360 tax ID	*
[Ref.: #20218]	Streptococcus cremoris 16S ribosomal RNA	M58836	1496	ENA	1359 tax ID	*
[Ref.: #20218]	Lactococcus lactis subsp. cremoris strain NIZO2418T 16S ribosomal RNA gene, partial sequence	EU091472	519	ENA	1359 tax ID	*
[Ref.: #20218]	Lactococcus lactis subsp. cremoris gene for 16S rRNA, partial sequence, strain: NBRC 100676	AB681219	1466	ENA	1359 tax ID	*
[Ref.: #20218]	Lactococcus lactis subsp. cremoris gene for 16S rRNA, partial sequence, strain: NCDO 607T	AB100802	1499	ENA	1359 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lactococcus cremoris subsp. cremoris NBRC 100676	GCA_001591705	contig	GenBank	1348655 tax ID
[Ref.: #67770]	Lactococcus cremoris LMG6897	GCA_001622295	contig	GenBank	1359 tax ID
[Ref.: #67770]	Lactococcus cremoris ATCC 19257	GCA_004354515	scaffold	GenBank	1359 tax ID
[Ref.: #66792]	Lactococcus lactis subsp. cremoris NBRC 100676	1348655.3	wgs	PATRIC	1348655 tax ID	*
[Ref.: #66792]	Lactococcus cremoris subsp. cremoris HP	GCA_000534815	contig	GenBank	1295827 tax ID	*
[Ref.: #66792]	Lactococcus lactis subsp. cremoris strain LMG6897	1359.37	wgs	PATRIC	1359 tax ID	*

[Ref.: #8499]	GC-content	35.4 mol%
[Ref.: #67770]	GC-content	34.8-35.6 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lactococcus lactis subsp. cremoris NBRC 100676 PATRIC: 1348655.3
[Ref.: #69480]	motile	no	95.845	no
[Ref.: #69480]	gram-positive	yes	94.11	no
[Ref.: #69480]	anaerobic	no	94.888	no
[Ref.: #69480]	halophile	yes	73.802	no
[Ref.: #69480]	spore-forming	no	93.622	no
[Ref.: #69480]	thermophile	no	99.623	yes
[Ref.: #69480]	glucose-util	yes	90.001	no
[Ref.: #69480]	aerobic	no	97.193	yes
[Ref.: #69480]	flagellated	no	96.999	no
[Ref.: #69480]	glucose-ferment	yes	89.268	no

Availability in culture collections External links

[Ref.: #8499]

Culture collection no.

DSM 20069, ATCC 19257, NCDO 607, NCIB 8662, CCUG 21953, LMG 6897, IMET 10707, JCM 16167, BCRC 12586, CECT 7100, CIP 102301, CNCTC 6910, HAMBI 1588, NBRC 100676, NCFB 607, NCIMB 8662

[Ref.: #83814]

SI-ID 92452

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Elevation of Lactococcus lactis subsp. cremoris to the species level as Lactococcus cremoris sp. nov. and transfer of Lactococcus lactis subsp. tructae to Lactococcus cremoris as Lactococcus cremoris subsp. tructae comb. nov.	Li TT, Tian WL, Gu CT	Int J Syst Evol Microbiol	10.1099/ijsem.0.004727	2021	*
Phylogeny	Quantification by real-time PCR of Lactococcus lactis subsp. cremoris in milk fermented by a mixed culture.	Grattepanche F, Lacroix C, Audet P, Lapointe G	Appl Microbiol Biotechnol	10.1007/s00253-004-1705-4	2004	*

References

#8499

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20069

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#42090 ; Curators of the CIP;
#47521 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 21953

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68381

Automatically annotated from API rID32STR .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#83814

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID92452.1 )

#118783 Collection of Institut Pasteur ; Curators of the CIP; CIP 102301
* These data were automatically processed and therefore are not curated

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Lactococcus lactis subsp. cremoris NBRC 100676

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Change proposal