[Ref.: #11750] |
Culture medium |
MIDDLEBROOK MEDIUM (DSMZ Medium 645), 37°C | |
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[Ref.: #11750] |
Culture medium growth |
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[Ref.: #11750] |
Culture medium link |
medium recipe provided by DSMZ |
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[Ref.: #19868] |
Culture medium |
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[Ref.: #19868] |
Culture medium growth |
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[Ref.: #19868] |
Culture medium composition |
expand / minimize |
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Name: Middlebrook 7H10 (MB7H10) Broth Base
Composition: amount in percent
Middlebrook 7H10 Agar 1.9%
Glycerol 0.5%
OADC-Enrichment in ml (NL5633) 10ml
Comments: Add broth base and glycerol to ddh2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-stock.
Sterilisation: 20 minutes Temperature:121°C
PH before:
PH after:
Biological details Culture type:
suitable for: Mycobacteria
OADC stock solution
old broth-name: OADC-Stock
Composition:
Bovine Serum Albumin, fraction V 5 %
1% Oleic Acid in 0.2N NaOH 5ml
Glucose 2 %
NaCl 0.85 %
Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this.
Sterilisation: 20 minutes Temperature:121°C
PH before:
PH after:
Biological details Culture type:
suitable for: Mycobacteria
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[Ref.: #19868] |
Culture medium |
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[Ref.: #19868] |
Culture medium growth |
 |
[Ref.: #19868] |
Culture medium composition |
expand / minimize |
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Name: Middlebrook 7H11 (MB7H11) Broth Base
Composition:
Middlebrook 7H11 Agar 2%
Glycerol 0.5%
OADC-Enrichment 10ml
Comments: Add broth base and glycerol to ddH2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-Stock.
Sterilisation: 20 minutes Temperature:121°C
PH before:
PH after:
Biological details Culture type:
suitable for: Mycobacteria
OADC stock solution
old broth-name: OADC-Stock
Composition:
Bovine Serum Albumin, fraction V 5 %
1% Oleic Acid in 0.2N NaOH 5ml
Glucose 2 %
NaCl 0.85 %
Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this.
Sterilisation: 20 minutes Temperature:121°C
PH before:
PH after:
Biological details Culture type:
suitable for: Mycobacteria
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[Ref.: #19868] |
Culture medium |
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[Ref.: #19868] |
Culture medium growth |
 |
[Ref.: #19868] |
Culture medium composition |
expand / minimize |
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Medium: 5006
Name:
Composition (g/l)
Sucrose 3,0
Dextrin 15,0
Meat extract 1,0
Yeast extract 2,0
Tryptone soy broth (Oxoid) 5,0
NaCl 0,5
K2HPO4 0,5
MgSO4 x 7 H2O 0,5
FeSO4 x 7 H2O 0,01
Agar 20,0
Preparation:
Sterilisation:
20 minutes at 121°C
pH before sterilisation:
7,3
Usage:
Maintenance
Organisms:
All Actinomycetes
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