| [Ref.: #4844] |
Culture medium |
| TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92), 28°C |
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| [Ref.: #4844] |
Culture medium growth |
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| [Ref.: #4844] |
Culture medium link |
medium recipe provided by DSMZ |
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| [Ref.: #18371] |
Culture medium |
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| [Ref.: #18371] |
Culture medium growth |
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| [Ref.: #18371] |
Culture medium composition |
expand / minimize |
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Name: ISP 2 / Yeast Malt Agar (5265); 5265
Composition
Malt extract 10.0 g/l
Yeast extract 4.0 g/l
Glucose 4.0 g/l
Agar 15.0 g/l
Preparation:
Sterilisation: 20 minutes at 121°C
pH before sterilisation: 7.0
Usage: Maintenance and Taxonomy
Organisms: All Actinomycetes
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| |
| [Ref.: #18371] |
Culture medium |
|
| [Ref.: #18371] |
Culture medium growth |
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| [Ref.: #18371] |
Culture medium composition |
expand / minimize |
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Name: ISP 3; 5315
Composition
Dog oat flakes 20.0 g/l
Trace element solution (5314) 2.5 ml/l
Agar 18.0 g/l
Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated).
Sterilisation: 20 minutes at 121°C
pH before sterilisation: 7.8
Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production)
Organisms: All Actinomycetes
Trace element solution 5314
Name: Trace element solution 5314; 5314
Composition
CaCl2 x H2O 3.0 g/l
Fe-III-citrate 1.0 g/l
MnSO4 0.2 g/l
ZnCl2 0.1 g/l
CuSO4 x 5 H2O 0.025 g/l
Sodium tetra borate 0.2 g/l
CoCl2 x 6 H2O 0.004 g/l
Sodium molybdate 0.01 g/l
Preparation: Use double destillated water.
Sterilisation: 20 minutes at 121°C
pH before sterilisation:
Usage: Trace element solution for different media
Organisms:
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| |
| [Ref.: #18371] |
Culture medium |
|
| [Ref.: #18371] |
Culture medium growth |
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| [Ref.: #18371] |
Culture medium composition |
expand / minimize |
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Name: ISP 4; DSM 547
Solution I:
Difco soluble starch, 10.0 g.
Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml.
Solution II:
CaCO3 2.0 g
K2HPO4 (anhydrous) 1.0 g
MgSO4 x 7 H2O 1.0 g
NaCl 1.0 g
(NH4)2SO4 2.0 g
Distilled water 500.0 ml
Trace salt solution (see below) 1.0 ml
The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min.
Trace element solution:
FeSO4 x 7 H2O 0.1 g
MnCl2 x 4 H2O 0.1 g
ZnSO4 x 7 H2O 0.1 g
Distilled water 100.0 ml
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| |
| [Ref.: #18371] |
Culture medium |
|
| [Ref.: #18371] |
Culture medium growth |
|
| [Ref.: #18371] |
Culture medium composition |
expand / minimize |
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Name: ISP 5 (5323)
Composition
L-Asparagine 1.0 g/l
Glycerol 10.0 g/l
K2HPO4 1.0 g/l
Salt solution (see preparation) 1.0 ml/l
Agar 20.0 g/l
Preparation:
Salt solution
1.0 g FeSO4 x 7 H2O
1.0 g MnCl2 x 4 H2O
1.0 g ZNSO4 x 7 H2O
in 100 ml water
Sterilisation:
20 minutes at 121°C
pH before sterilisation:
7.2
Usage:
Maintenance and taxonomy
Organisms:
All Actinomycetes
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| |
| [Ref.: #18371] |
Culture medium |
|
| [Ref.: #18371] |
Culture medium growth |
|
| [Ref.: #18371] |
Culture medium composition |
expand / minimize |
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Name: ISP 6 (5318)
Composition
Peptone 15.0 g/l
Proteose peptose 5.0 g/l
Ferric ammonium citrate 0.5 g/l
Sodium glycerophosphate 1.0 g/l
Sodium thiosulfate 0.08 g/l
Yeast extract 1.0 g/l
Agar 15.0 g/l
Sterilisation:
20 minutes at 121°C
pH before sterilisation:
Usage: Production of melanoid pigments
Organisms:
All Actinomycetes
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| |
| [Ref.: #18371] |
Culture medium |
|
| [Ref.: #18371] |
Culture medium growth |
|
| [Ref.: #18371] |
Culture medium composition |
expand / minimize |
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Name: ISP 7 (5322)
Composition
Glycerol 15.0 g/l
L-Tyrosine 0.5 g/l
L-Asparagine 1.0 g/l
K2HPO4 0.5 g/l
NaCl 0.5 g/l
FeSO4 x 7 H2O 0.01 g/l
Trace element solution 5343 1.0 ml/l
Agar 20.0
Sterilisation:
20 minutes at 121°C
pH before sterilisation:
7.3
Usage:
Production of melanoid pigments
Organisms:
All Actinomycetes
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| [Ref.: #41906] |
Culture medium |
| MEDIUM 368 - for Luria bertani | |
|
| [Ref.: #41906] |
Culture medium growth |
|
| [Ref.: #41906] |
Culture medium composition |
expand / minimize |
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Distilled water make up to (1000.000 ml);Sodium chloride (10.000 g);Agar(15.000 g);Yeast extract (5.000 g);Tryptone (10.000 g)
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