Name: Priestia megaterium DSM 319
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 717

Type strain:

Species: Priestia megaterium

Culture col. no.: DSM 319

Strain history: <- IMG 1521

NCBI tax ID(s): 592022 (strain), 1404 (species)

SI-ID 47142

DSM 319

Other strains from Priestia megaterium

Section

Priestia megaterium DSM 319 is a spore-forming, mesophilic, motile human pathogen of the family Bacillaceae.

motile
spore-forming
human pathogen
mesophilic
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Priestia*
	Species	**Priestia megaterium**
	Full Scientific Name (LPSN)	Priestia megaterium (de Bary 1884) Gupta et al. 2020

Synonym

Bacillus megaterium

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	yes Confidence in %94.545

Information on culture and growth conditions Culture and growth conditions

[Ref.: #105]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #105]

Culture medium growth

[Ref.: #105]

Culture medium link

Medium recipe at MediaDive

[Ref.: #105]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #105]			growth	30 °C

[Ref.: #105]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #105]	Pathogenicity (human)	yes, in single cases
[Ref.: #105]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Priestia megaterium DSM 319	GCA_000025805	complete	GenBank	592022 tax ID	*
[Ref.: #66792]	Bacillus megaterium DSM 319	592022.4	complete	PATRIC	592022 tax ID	*
[Ref.: #66792]	Bacillus megaterium DSM 319	646564507	complete	JGI IMG/M	592022 tax ID	*
[Ref.: #66792]	Priestia megaterium DSM 319	GCA_029537015	complete	GenBank	592022 tax ID	*

[Ref.: #105]

GC-content

38.1 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Priestia megaterium DSM 319 DSM 319 NCBI: GCA_000025805.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Priestia megaterium DSM 319 DSM 319 NCBI: GCA_000025805.1
[Ref.: #69480]	motile	yes	86.659	no
[Ref.: #69480]	flagellated	yes	85.374	no
[Ref.: #69480]	gram-positive	yes	88.786	no
[Ref.: #69480]	anaerobic	no	97.038	no
[Ref.: #69480]	aerobic	yes	87.445	no
[Ref.: #69480]	halophile	no	59.105	no
[Ref.: #69480]	spore-forming	yes	93.57	no
[Ref.: #69480]	thermophile	no	96.976	yes
[Ref.: #69480]	glucose-util	yes	93.35	no
[Ref.: #69480]	glucose-ferment	no	88.217	no

Availability in culture collections External links

[Ref.: #105]

Culture collection no.

DSM 319

[Ref.: #70392]

SI-ID 47142

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Insights into polymer versus oligosaccharide synthesis: mutagenesis and mechanistic studies of a novel levansucrase from Bacillus megaterium.	Homann A, Biedendieck R, Gotze S, Jahn D, Seibel J	Biochem J	10.1042/BJ20070600	2007	*
Biotechnology	A Systems-Based Approach for Cyanide Overproduction by Bacillus megaterium for Gold Bioleaching Enhancement.	Aminian-Dehkordi J, Mousavi SM, Marashi SA, Jafari A, Mijakovic I	Front Bioeng Biotechnol	10.3389/fbioe.2020.00528	2020	*
Phylogeny	Phage vB_BmeM-Goe8 infecting Bacillus megaterium DSM319.	Baena Lozada LP, Hoppert M, Hertel R	Arch Virol	10.1007/s00705-019-04513-5	2019	*
Genetics	Manually curated genome-scale reconstruction of the metabolic network of Bacillus megaterium DSM319.	Aminian-Dehkordi J, Mousavi SM, Jafari A, Mijakovic I, Marashi SA	Sci Rep	10.1038/s41598-019-55041-w	2019	*
Metabolism	CYP109E1 from Bacillus megaterium Acts as a 24- and 25-Hydroxylase for Cholesterol.	Putkaradze N, Litzenburger M, Hutter MC, Bernhardt R	Chembiochem	10.1002/cbic.201800595	2019	*
Enzymology	Biochemical and structural characterization of CYP109A2, a vitamin D3 25-hydroxylase from Bacillus megaterium.	Abdulmughni A, Jozwik IK, Brill E, Hannemann F, Thunnissen AWH, Bernhardt R	FEBS J	10.1111/febs.14276	2017	*
Metabolism	Structural basis of steroid binding and oxidation by the cytochrome P450 CYP109E1 from Bacillus megaterium.	Jozwik IK, Kiss FM, Gricman L, Abdulmughni A, Brill E, Zapp J, Pleiss J, Bernhardt R, Thunnissen AW	FEBS J	10.1111/febs.13911	2016	*
Enzymology	Identification of new substrates for the CYP106A1-mediated 11-oxidation and investigation of the reaction mechanism.	Kiss FM, Khatri Y, Zapp J, Bernhardt R	FEBS Lett	10.1016/j.febslet.2015.07.011	2015	*
Metabolism	Comparison of CYP106A1 and CYP106A2 from Bacillus megaterium - identification of a novel 11-oxidase activity.	Kiss FM, Schmitz D, Zapp J, Dier TK, Volmer DA, Bernhardt R	Appl Microbiol Biotechnol	10.1007/s00253-015-6563-8	2015	*
Metabolism	Towards a cell factory for vitamin B12 production in Bacillus megaterium: bypassing of the cobalamin riboswitch control elements.	Moore SJ, Mayer MJ, Biedendieck R, Deery E, Warren MJ	N Biotechnol	10.1016/j.nbt.2014.03.003	2014	*
Enzymology	Extracellular L-asparaginase from a protease-deficient Bacillus aryabhattai ITBHU02: purification, biochemical characterization, and evaluation of antineoplastic activity in vitro.	Singh Y, Gundampati RK, Jagannadham MV, Srivastava SK	Appl Biochem Biotechnol	10.1007/s12010-013-0455-0	2013	*
Metabolism	A new cytochrome P450 system from Bacillus megaterium DSM319 for the hydroxylation of 11-keto-beta-boswellic acid (KBA).	Brill E, Hannemann F, Zapp J, Bruning G, Jauch J, Bernhardt R	Appl Microbiol Biotechnol	10.1007/s00253-013-5029-0	2013	*
Enzymology	Characterization of the enzyme CbiH60 involved in anaerobic ring contraction of the cobalamin (vitamin B12) biosynthetic pathway.	Moore SJ, Biedendieck R, Lawrence AD, Deery E, Howard MJ, Rigby SE, Warren MJ	J Biol Chem	10.1074/jbc.M112.422535	2012	*
Genetics	Genome sequences of the biotechnologically important Bacillus megaterium strains QM B1551 and DSM319.	Eppinger M, Bunk B, Johns MA, Edirisinghe JN, Kutumbaka KK, Koenig SS, Creasy HH, Rosovitz MJ, Riley DR, Daugherty S, Martin M, Elbourne LD, Paulsen I, Biedendieck R, Braun C, Grayburn S, Dhingra S, Lukyanchuk V, Ball B, Ul-Qamar R, Seibel J, Bremer E, Jahn D, Ravel J, Vary PS	J Bacteriol	10.1128/JB.00449-11	2011	*
Metabolism	Functional analysis of the response regulator DegU in Bacillus megaterium DSM319 and comparative secretome analysis of degSU mutants.	Borgmeier C, Voigt B, Hecker M, Meinhardt F	Appl Microbiol Biotechnol	10.1007/s00253-011-3302-7	2011	*
Enzymology	[Cloning, expression and the characterization of beta-amylase from a Bacillus megaterium WS06].	Wu J, Zhang S	Sheng Wu Gong Cheng Xue Bao		2008	*
Biotechnology	Bacillus megaterium--from simple soil bacterium to industrial protein production host.	Vary PS, Biedendieck R, Fuerch T, Meinhardt F, Rohde M, Deckwer WD, Jahn D	Appl Microbiol Biotechnol	10.1007/s00253-007-1089-3	2007	*
Phylogeny	Comparative study on central metabolic fluxes of Bacillus megaterium strains in continuous culture using 13C labelled substrates.	Furch T, Hollmann R, Wittmann C, Wang W, Deckwer WD	Bioprocess Biosyst Eng	10.1007/s00449-006-0095-7	2006	*
Biotechnology	Comparative proteomic analysis of high cell density cultivations with two recombinant Bacillus megaterium strains for the production of a heterologous dextransucrase.	Wang W, Hollmann R, Deckwer WD	Proteome Sci	10.1186/1477-5956-4-19	2006	*
Genetics	A protein database constructed from low-coverage genomic sequence of Bacillus megaterium and its use for accelerated proteomic analysis.	Sun J, Wang W, Hundertmark C, Zeng AP, Jahn D, Deckwer WD	J Biotechnol	10.1016/j.jbiotec.2006.01.033	2006	*
Genetics	Evidence for two recA genes mediating DNA repair in Bacillus megaterium.	Nahrstedt H, Schroder C, Meinhardt F	Microbiology (Reading)	10.1099/mic.0.27626-0	2005	*
Metabolism	Identification and functional characterization of a type I signal peptidase gene of Bacillus megaterium DSM319.	Nahrstedt H, Wittchen K, Rachman MA, Meinhardt F	Appl Microbiol Biotechnol	10.1007/s00253-003-1469-2	2003	*
Enzymology	Cloning, expression, and carbon catabolite repression of the bamM gene encoding beta-amylase of Bacillus megaterium DSM319.	Lee JS, Wittchen KD, Stahl C, Strey J, Meinhardt F	Appl Microbiol Biotechnol	10.1007/s002530100645	2001	*
Metabolism	Regulation of beta-galactosidase expression in Bacillus megaterium DSM319 by a XylS/AraC-type transcriptional activator.	Strey J, Wittchen KD, Meinhardt F	J Bacteriol	10.1128/JB.181.10.3288-3292.1999	1999	*
Enzymology	Plasmids for efficient single-copy gene cloning into gdh2 and trpC of Bacillus megaterium DSM319 and QM B1551.	Schmiedel D, Vary PS, Jablonski L, Hillen W	Appl Microbiol Biotechnol	10.1007/s002530050970	1997	*
Enzymology	Inactivation of the major extracellular protease from Bacillus megaterium DSM319 by gene replacement.	Wittchen KD, Meinhardt F	Appl Microbiol Biotechnol	10.1007/BF00191184	1995	*
Enzymology	Cloning and sequencing of the leu C and npr M genes and a putative spo IV gene from Bacillus megaterium DSM319.	Meinhardt F, Busskamp M, Wittchen KD	Appl Microbiol Biotechnol	10.1007/BF00221230	1994	*
Enzymology	Cloning and sequencing of the genes encoding glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase and triosephosphate isomerase (gap operon) from mesophilic Bacillus megaterium: comparison with corresponding sequences from thermophilic Bacillus stearothermophilus.	Schlapfer BS, Zuber H	Gene	10.1016/0378-1119(92)90031-j	1992	*
	First Report of a Leaf Spot of Radermachera sinica in China Caused by Bacillus megaterium.	Li YL, Zhou Z, Yuan YC, Ye JR	Plant Dis	10.1094/PDIS-05-14-0469-PDN	2014	*
	Molecular characterization of the operon comprising the spoIV gene of Bacillus megaterium DSM319 and generation of a deletion mutant.	Wittchen KD, Strey J, Bultmann A	J Gen Appl Microbiol	10.2323/jgam.44.317	1998	*

References

#105	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 319

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70392

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID47142.1 )

* These data were automatically processed and therefore are not curated

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Priestia megaterium DSM 319 DSM 319

Priestia megaterium DSM 319 DSM 319

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