Lactobacillus kefiranofaciens subsp. kefiranofaciens WT-2B | Type strain | DSM 5016, ATCC 43761, JCM 6985

Strain identifier

BacDive ID: 6622

Type strain:

Species: Lactobacillus kefiranofaciens subsp. kefiranofaciens

Strain Designation: WT-2B

Culture col. no.: DSM 5016, ATCC 43761, JCM 6985

Section

Name and taxonomic classification
Morphology and physiology
Culture and growth conditions
Isolation, sampling and environmental information
Application and interaction
Molecular biology
Strain availability

External links

Information on the name and the taxonomic classification.Name and taxonomic classification

[Ref.: #1909]	Domain	Bacteria
[Ref.: #1909]	Phylum	Firmicutes
[Ref.: #1909]	Class	Bacilli
[Ref.: #1909]	Order	Lactobacillales
[Ref.: #1909]	Family	Lactobacillaceae
[Ref.: #1909]	Genus	Lactobacillus
[Ref.: #1909]	Species	Lactobacillus kefiranofaciens subsp. kefiranofaciens
[Ref.: #1909]	Full Scientific Name	Lactobacillus kefiranofaciens subsp. kefiranofaciens Fujisawa et al. 1988
[Ref.: #1909]	Strain Designation	WT-2B
[Ref.: #1909]	Type strain

		Prokaryotic Nomenclature Up-to-date (PNU)	Taxonomical status	Literature reference
[Ref.: #20215]	Domain	Bacteria
[Ref.: #20215]	Phylum	Firmicutes
[Ref.: #20215]	Class	Bacilli		Int. J. Syst. Evol. Microbiol. 60:469
[Ref.: #20215]	Family	Lactobacillaceae		Int. J. Syst. Bacteriol. 30:236
[Ref.: #20215]	Genus	Lactobacillus	genus (AL)	Int. J. Syst. Bacteriol. 30:310 (AL)
[Ref.: #20215]	Species	Lactobacillus kefiranofaciens subsp. kefiranofaciens	subsp. nov. (VP)	Int. J. Syst. Bacteriol. 38:12*
[Ref.: #20215]	Full Scientific Name	Lactobacillus kefiranofaciens subsp. kefiranofaciens Fujisawa et al. 1988

[Ref.: #20215]

Synonym

Lactobacillus kefiranofaciens

Information on morphological and physiological propertiesMorphology and physiology

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #1909]	1490	-	-	-	-	-	+	-	-	-	-	+	+	+	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-

[Ref.: #1909]

Oxygen tolerance

anaerobe

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #1909]

Culture medium

KPL MEDIUM (DSMZ Medium 456), 28°C, anaerobic

[Ref.: #1909]

Culture medium growth

[Ref.: #1909]

Culture medium link

medium recipe provided by DSMZ

[Ref.: #34032]

Culture medium

MEDIUM 184 - for Lactobacillus kefiranofaciens

[Ref.: #34032]

Culture medium growth

[Ref.: #34032]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #1909]			growth	28 ̊C
[Ref.: #34032]			growth	30 ̊C

[Ref.: #1909]

Temperature range

mesophilic

Information on isolation source, the sampling and environmental conditionsIsolation, sampling and environmental information

[Ref.: #1909]

Sample type/isolated from

kefir grains

Isolation sources categories

#Engineered

#Food production

What are isolation sources categories?

* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hostsApplication and interaction

[Ref.: #1909]

Biosafety level

Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databassMolecular biology

[Ref.: #1909]

GC-content

34.3 mol%

Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence database	Sequence accession description	Sequence accession number	Sequence length (bp)	Associated NCBI tax ID
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus kefiranofaciens ATCC 43761 16S ribosomal RNA gene, partial sequence	AF429517	487	267818	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus kefiranofaciens strain ATCC 43761 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF429604	560	267818	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus kefiranofaciens strain ATCC 43761 60 kDa heat shock protein (HSP60) gene, partial cds	AF429691	552	267818	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus kefiranofaciens strain BCRC 16059 DNA gyrase subunit B (gyrB) gene, partial cds	EU647683	777	267818	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Lactobacillus kefiranofaciens subsp. kefiranofaciens strain BCRC 16059 recombinase A gene, partial cds	JN226858	302	190905	*
[Ref.: #20218]	EMBL Genome project data	Lactobacillus kefiranofaciens subsp. kefiranofaciens partial 16S rRNA gene, type strain ATCC 43761T	FR683092	1549	190905	*
[Ref.: #20218]	EMBL Genome project data	Lactobacillus kefiranofaciens subsp. kefiranofaciens partial pheS gene for phenylalanine tRNA synthetase, alpha subunit, type strain CIP 103307T	FR775933	462	190905	*
[Ref.: #20218]	EMBL Genome project data	Lactobacillus kefiranofaciens subsp. kefiranofaciens partial rpoA gene for RNA polymerase, alpha subunit, type strain CIP 103307T	FR775946	671	190905	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Lactobacillus kefiranofaciens subsp. kefiranofaciens partial 16S rRNA gene, strain type strain:DSM 5016	AM113781	1552	190905	*
[Ref.: #20218]	Marker Gene (DDBJ Direct submission)	Lactobacillus kefiranofaciens subsp. kefiranofaciens gene for 16S rRNA, partial sequence, strain: JCM 6985	AB289182	651	190905	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Lactobacillus kefiranofaciens subsp. kefiranofaciens 16S rRNA gene, strain LMG 19149	AJ575259	1516	190905	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Lactobacillus kefiranofaciens subsp. kefiranofaciens pheS gene for phenylalanyl-tRNA synthase alpha subunit, type strain LMG 19149T	AM087745	455	190905	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Lactobacillus kefiranofaciens subsp. kefiranofaciens rpoA gene for RNA polymerase alpha subunit, type strain LMG 19149T	AM087847	610	190905	*
[Ref.: #1909]	INSDC (WGS)		AZGG00000000

Availability in culture collectionsStrain availability

[Ref.: #1909]

Culture collection no.

DSM 5016, ATCC 43761, JCM 6985

[Ref.: #1909]

Strain history

<- JCM <- S. Adachi et al., WT-2B

[Ref.: #20218]

Associated Passport(s) in StrainInfo

13821, 13820, 13822

References

#1909

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 5016

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#34032 ; Curators of the CIP;
* These References are textmined

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