Brachybacterium faecium 6-10 | Type strain | DSM 4810, ATCC 43885, IMET 11352, NCIB 9860

Strain identifier

BacDive ID: 3902

Type strain:

Species: Brachybacterium faecium

Strain Designation: 6-10

Culture col. no.: DSM 4810, ATCC 43885, IMET 11352, NCIB 9860

Section

Name and taxonomic classification
Morphology and physiology
Culture and growth conditions
Isolation, sampling and environmental information
Application and interaction
Molecular biology
Strain availability

External links

Information on the name and the taxonomic classification.Name and taxonomic classification

[Ref.: #1836]	Domain	Bacteria
[Ref.: #1836]	Phylum	Actinobacteria
[Ref.: #1836]	Class	Actinobacteria
[Ref.: #1836]	Order	Actinomycetales
[Ref.: #1836]	Family	Dermabacteraceae
[Ref.: #1836]	Genus	Brachybacterium
[Ref.: #1836]	Species	Brachybacterium faecium
[Ref.: #1836]	Full Scientific Name	Brachybacterium faecium Collins et al. 1988
[Ref.: #1836]	Strain Designation	6-10
[Ref.: #1836]	Type strain

		Prokaryotic Nomenclature Up-to-date (PNU)	Taxonomical status	Literature reference
[Ref.: #20215]	Domain	Bacteria
[Ref.: #20215]	Phylum	Actinobacteria
[Ref.: #20215]	Class	Actinobacteria		Int. J. Syst. Bacteriol. 47:483*
[Ref.: #20215]	Family	Dermabacteraceae		Int. J. Syst. Bacteriol. 47:485*
[Ref.: #20215]	Genus	Brachybacterium	gen. nov. (VP)	Int. J. Syst. Bacteriol. 38:45*
[Ref.: #20215]	Species	Brachybacterium faecium	sp. nov. (VP)	Int. J. Syst. Bacteriol. 38:45*
[Ref.: #20215]	Full Scientific Name	Brachybacterium faecium Collins et al. 1988

Information on morphological and physiological propertiesMorphology and physiology

[Ref.: #20197]	Colony color	Light ivory (1015)
[Ref.: #20197]	Incubation period	10-14 days
[Ref.: #20197]	Cultivation medium used	ISP 2

[Ref.: #20197]	Colony color	Light ivory (1015)
[Ref.: #20197]	Incubation period	10-14 days
[Ref.: #20197]	Cultivation medium used	ISP 4

[Ref.: #20197]	Colony color	Light ivory (1015)
[Ref.: #20197]	Incubation period	10-14 days
[Ref.: #20197]	Cultivation medium used	ISP 5

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #20197]		NaCl		maximum	7.5	%

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #20197]	640	+	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #20197]	466	+/-	+	+	-	+	+	+	-	-	-	+/-	not determinedn.d.	+	+	-	+/-	-	+	+	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #20197]	976	not determinedn.d.	+	+	+	-	+	+	+	-	-	-	+	+	+	-	+	-	+	+	-

	Metabolite	Utilization activity
[Ref.: #20197]	Arabinose	+
[Ref.: #20197]	Cellulose	+
[Ref.: #20197]	Fructose	+/-
[Ref.: #20197]	Glucose	+
[Ref.: #20197]	Inositol	+
[Ref.: #20197]	Mannitol	+/-
[Ref.: #20197]	Raffinose	+
[Ref.: #20197]	Rhamnose	+
[Ref.: #20197]	Sucrose	+
[Ref.: #20197]	Xylose	+

[Ref.: #20197]	Multicellular complex forming ability	no
[Ref.: #20197]	Medium Name (multicellularity)	ISP 2

[Ref.: #20197]	Multicellular complex forming ability	no
[Ref.: #20197]	Medium Name (multicellularity)	ISP 4

[Ref.: #20197]	Multicellular complex forming ability	no
[Ref.: #20197]	Medium Name (multicellularity)	ISP 5

[Ref.: #1836]	Murein short key	A31.02
[Ref.: #1836]	Murein types	A4gamma m-Dpm-D-Glu₂; alpha-carboxyl group of D-Glu substituted by glycine

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #1836]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92), 30°C

[Ref.: #1836]

Culture medium growth

[Ref.: #1836]

Culture medium link

medium recipe provided by DSMZ

[Ref.: #20197]

Culture medium

ISP 2

[Ref.: #20197]

Culture medium growth

[Ref.: #20197]

Culture medium composition

expand / minimize

[Ref.: #20197]

Culture medium

ISP 4

[Ref.: #20197]

Culture medium growth

[Ref.: #20197]

Culture medium composition

expand / minimize

[Ref.: #20197]

Culture medium

ISP 5

[Ref.: #20197]

Culture medium growth

[Ref.: #20197]

Culture medium composition

expand / minimize

[Ref.: #40603]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #40603]

Culture medium growth

[Ref.: #40603]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #1836]	growth	30 ̊C
[Ref.: #20197]	optimum	28 ̊C
[Ref.: #40603]	growth	30 ̊C

[Ref.: #1836]	Temperature range	mesophilic
[Ref.: #20197]	Temperature range	mesophilic

Information on isolation source, the sampling and environmental conditionsIsolation, sampling and environmental information

[Ref.: #1836]

Sample type/isolated from

poultry deep litter

Isolation sources categories

#Engineered	#Waste	-
#Host-associates	#Birds	-
#Host-associates	#Other	#Decomposing animals

What are isolation sources categories?

* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hostsApplication and interaction

[Ref.: #1836]	Biosafety level	1	Risk group (German classification)
[Ref.: #20197]	Biosafety level	1

Information on genomic background e.g. entries in nucleic sequence databassMolecular biology

[Ref.: #1836]

GC-content

69.4 mol%

thermal denaturation, midpoint method (Tm)

Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence database	Sequence accession description	Sequence accession number	Sequence length (bp)	Associated NCBI tax ID
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Brachybacterium faecium DSM 4810 strain ATCC 43885 60 kDa chaperonin (cpn60) gene, partial cds	AY691214	552	446465	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	TPA: Brachybacterium faecium DSM 4810 transfer-messenger mRNA Brach_faeci_4810, single chain mature transcript	HG783529	371	446465	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	JP 2011142867-A/21: Site-specific genetic recombination method based on TG1 integrase	HV455539	46	446465	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	B.faecium 16S rRNA gene	X83810	1480	446465	*
[Ref.: #1836]	EMBL Direct submission	B.faecium 16S ribosomal RNA gene	X91032	1513	446465
[Ref.: #1836]	GenBank Genome project data	Brachybacterium faecium DSM 4810 strain 6-10, complete genome	CP001643	3614992	446465

Availability in culture collectionsStrain availability

[Ref.: #1836]

Culture collection no.

DSM 4810, ATCC 43885, IMET 11352, NCIB 9860

[Ref.: #1836]

Strain history

<- NCIB <- H.E. Schefferle, 6-10

[Ref.: #20218]

Associated Passport(s) in StrainInfo

58416, 58413, 58418, 113273

References

#1836

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 4810

#20197

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40603 ; Curators of the CIP;
* These References are textmined

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