[Ref.: #1836] |
Culture medium |
TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92), 30°C |
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[Ref.: #1836] |
Culture medium growth |
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[Ref.: #1836] |
Culture medium link |
medium recipe provided by DSMZ |
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[Ref.: #20197] |
Culture medium |
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[Ref.: #20197] |
Culture medium growth |
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[Ref.: #20197] |
Culture medium composition |
expand / minimize |
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Name: ISP 2 / Yeast Malt Agar (5265); 5265
Composition
Malt extract 10.0 g/l
Yeast extract 4.0 g/l
Glucose 4.0 g/l
Agar 15.0 g/l
Preparation:
Sterilisation: 20 minutes at 121°C
pH before sterilisation: 7.0
Usage: Maintenance and Taxonomy
Organisms: All Actinomycetes
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[Ref.: #20197] |
Culture medium |
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[Ref.: #20197] |
Culture medium growth |
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[Ref.: #20197] |
Culture medium composition |
expand / minimize |
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Name: ISP 4; DSM 547
Solution I:
Difco soluble starch, 10.0 g.
Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml.
Solution II:
CaCO3 2.0 g
K2HPO4 (anhydrous) 1.0 g
MgSO4 x 7 H2O 1.0 g
NaCl 1.0 g
(NH4)2SO4 2.0 g
Distilled water 500.0 ml
Trace salt solution (see below) 1.0 ml
The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min.
Trace element solution:
FeSO4 x 7 H2O 0.1 g
MnCl2 x 4 H2O 0.1 g
ZnSO4 x 7 H2O 0.1 g
Distilled water 100.0 ml
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[Ref.: #20197] |
Culture medium |
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[Ref.: #20197] |
Culture medium growth |
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[Ref.: #20197] |
Culture medium composition |
expand / minimize |
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Name: ISP 5 (5323)
Composition
L-Asparagine 1.0 g/l
Glycerol 10.0 g/l
K2HPO4 1.0 g/l
Salt solution (see preparation) 1.0 ml/l
Agar 20.0 g/l
Preparation:
Salt solution
1.0 g FeSO4 x 7 H2O
1.0 g MnCl2 x 4 H2O
1.0 g ZNSO4 x 7 H2O
in 100 ml water
Sterilisation:
20 minutes at 121°C
pH before sterilisation:
7.2
Usage:
Maintenance and taxonomy
Organisms:
All Actinomycetes
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[Ref.: #40603] |
Culture medium |
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[Ref.: #40603] |
Culture medium growth |
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[Ref.: #40603] |
Culture medium composition |
expand / minimize |
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Columbia agar (39.000 g);distilled water (1000.000 ml)
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