Ralstonia pickettii K-288 | Type strain | DSM 6297, ATCC 27511, CIP 73.23, JCM 5969, NCTC 11149

Strain identifier

BacDive ID: 2085

Type strain:

Species: Ralstonia pickettii

Strain Designation: K-288

Culture col. no.: DSM 6297, ATCC 27511, CIP 73.23, JCM 5969, NCTC 11149

Section

Name and taxonomic classification
Morphology and physiology
Culture and growth conditions
Isolation, sampling and environmental information
Application and interaction
Molecular biology
Strain availability

External links

Information on the name and the taxonomic classification.Name and taxonomic classification

[Ref.: #2581]	Domain	Bacteria
[Ref.: #2581]	Phylum	Proteobacteria
[Ref.: #2581]	Class	Betaproteobacteria
[Ref.: #2581]	Order	Burkholderiales
[Ref.: #2581]	Family	Burkholderiaceae
[Ref.: #2581]	Genus	Ralstonia
[Ref.: #2581]	Species	Ralstonia pickettii
[Ref.: #2581]	Full Scientific Name	Ralstonia pickettii (Ralston et al. 1973) Yabuuchi et al. 1996
[Ref.: #2581]	Strain Designation	K-288
[Ref.: #2581]	Type strain

		Prokaryotic Nomenclature Up-to-date (PNU)	Taxonomical status	Literature reference
[Ref.: #20215]	Domain	Bacteria
[Ref.: #20215]	Phylum	Proteobacteria
[Ref.: #20215]	Class	Betaproteobacteria		Int. J. Syst. Evol. Microbiol. 56:1
[Ref.: #20215]	Family	Burkholderiaceae
[Ref.: #20215]	Genus	Ralstonia	gen. nov. (VL)	Int. J. Syst. Bacteriol. 46:625
[Ref.: #20215]	Species	Ralstonia pickettii	comb. nov. (VL)	Int. J. Syst. Bacteriol. 46:625
[Ref.: #20215]	Full Scientific Name	Ralstonia pickettii (Ralston et al. 1973) Yabuuchi et al. 1996

[Ref.: #20215]	Synonym	Burkholderia pickettii
[Ref.: #20215]	Synonym	Pseudomonas pickettii

Information on morphological and physiological propertiesMorphology and physiology

[Ref.: #22944]	Cell shape	rod-shaped
[Ref.: #22944]	Flagellum arrangement	monotrichous, polar

	Enzyme	Enzyme activity	EC number
[Ref.: #22944]	alkaline phosphatase	-	3.1.3.1
[Ref.: #22943]	catalase	-	1.11.1.6
[Ref.: #22944]	catalase	+	1.11.1.6
[Ref.: #22943]	cystine arylamidase	-	3.4.11.3
[Ref.: #22943]	cytochrome oxidase	-	1.9.3.1
[Ref.: #22944]	cytochrome oxidase	+	1.9.3.1
[Ref.: #22943]	esterase (C 4)	+/-
[Ref.: #22943]	lipase (C 14)	+
[Ref.: #22944]	phenylalanine deaminase	+	4.3.1.5
[Ref.: #22943]	phosphoamidase	+/-	3.9.1.1
[Ref.: #22944]	pyrrolidonyl arylamidase	+	3.4.19.3
[Ref.: #22943]	urease	+/-	3.5.1.5
[Ref.: #22944]	urease	+	3.5.1.5
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #2581]	3958	+	-	-	-	-	-	-	-	+	+	-	-	-	-	+	+	-	+	+	-	+
[Ref.: #2581]	3938	+	-	-	-	-	-	-	-	+	+	-	-	-	-	+	+	-	+	+	+	+

	Antibiotica	Metabolite	Antibiotic intermediate	Concentration
[Ref.: #22943]		Penicillin	yes	10	µg (disc)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #22944]	3-Hydroxybenzoate	-	growth
[Ref.: #22944]	Acetic acid	+	builds base from
[Ref.: #22943]	Adipic acid	+/-	growth
[Ref.: #22944]	Allantoin	-	builds base from
[Ref.: #22943]	Caprate	+/-	growth
[Ref.: #22944]	Caprate	+	growth
[Ref.: #22943]	Citric acid	+	growth
[Ref.: #22944]	Citric acid	+	builds base from
[Ref.: #22944]	D-Arabitol	-	builds acid from
[Ref.: #22943]	D-Fructose	+/-	fermentation
[Ref.: #22943]	D-Glucose	+	growth
[Ref.: #22943]	D-Glucose	+/-	fermentation
[Ref.: #22943]	D-Xylose	+/-	fermentation
[Ref.: #22944]	D-Xylose	+	builds acid from
[Ref.: #22944]	Galactaric acid	+	builds base from
[Ref.: #22944]	Galacturonate	+	builds base from
[Ref.: #22944]	Glucose	+	builds acid from
[Ref.: #22944]	Inositol	-	builds acid from
[Ref.: #22943]	L-Arabinose	+	growth
[Ref.: #22944]	L-Arabinose	+	builds acid from
[Ref.: #22944]	Lactic acid	+	builds base from
[Ref.: #22944]	Lactose	-	builds acid from
[Ref.: #22944]	Maleic acid	+	builds base from
[Ref.: #22944]	Malonate	+	builds base from
[Ref.: #22944]	Maltose	-	builds acid from
[Ref.: #22944]	Mannitol	-	builds acid from
[Ref.: #22943]	N-Acetyl-D-glucosamine	+	growth
[Ref.: #22943]	Nitrate	+	reduction
[Ref.: #22944]	Nitrate	+	reduction
[Ref.: #22944]	Oxalic acid	-	builds base from
[Ref.: #22943]	Phenylacetate	+/-	growth
[Ref.: #22944]	Propionic acid	+	growth
[Ref.: #22944]	Suberate	+	growth
[Ref.: #22944]	Sucrose	-	builds acid from
[Ref.: #22944]	Tartaric acid	+	builds base from
[Ref.: #22943]	Tween 80	+	growth
[Ref.: #22944]	Tween 80	+	hydrolysis
[Ref.: #22943]	Urea	+/-	hydrolysis
[Ref.: #22944]	Urea	+	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #2581]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1), 30°C

[Ref.: #2581]

Culture medium growth

[Ref.: #2581]

Culture medium link

medium recipe provided by DSMZ

[Ref.: #36535]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #36535]

Culture medium growth

[Ref.: #36535]

Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #2581]		growth	30 ̊C
[Ref.: #22943]	inconsistent	growth	42 ̊C
[Ref.: #22944]	inconsistent	growth	42 ̊C
[Ref.: #36535]		growth	30 ̊C

[Ref.: #2581]	Temperature range	mesophilic
[Ref.: #22943]	Temperature range	mesophilic
[Ref.: #22944]	Temperature range	mesophilic

Information on isolation source, the sampling and environmental conditionsIsolation, sampling and environmental information

[Ref.: #36535]	Country	USA
[Ref.: #36535]	Continent	North America
* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hostsApplication and interaction

[Ref.: #2581]

Biosafety level

Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databassMolecular biology

[Ref.: #22943]

GC-content

64 mol%

thermal denaturation, midpoint method (Tm)

Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence database	Sequence accession description	Sequence accession number	Sequence length (bp)	Associated NCBI tax ID
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Ralstonia pickettii 16S ribosomal RNA gene, partial sequence	AF467977	995	329	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Ralstonia pickettii strain ATCC 27511 Cpn60 (cpn60) gene, partial cds	AY123717	555	329	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Ralstonia pickettii strain ATCC 27511 16S ribosomal RNA gene, partial sequence	AY741342	1491	329	*
[Ref.: #20218]	Marker Gene (DDBJ Direct submission)	Ralstonia pickettii gene for 16S rRNA, partial sequence, strain: ATCC 27511 (= EY 1917)	D13047	158	329	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Ralstonia pickettii strain ATCC 27511 cation/multidrug efflux pump (agrA) gene, partial cds	JQ294037	434	329	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Ralstonia pickettii strain ATCC 27511 silver efflux protein (silA) gene, partial cds	JQ294048	513	329	*
[Ref.: #20218]	INSDC Sequence	Pseudomonas pickettii 16S ribosomal RNA (16S rRNA) gene, transfer RNA-Ile (tRNA-Ile) gene, transfer RNA-Ala (tRNA-Ala) gene, 23S ribosomal RNA (23S rRNA) gene	L28163	704	329	*
[Ref.: #20218]	INSDC Sequence	16S rRNA [Burkholderia pickettii, ATCC 27511, Genomic, 970 nt, segment 1 of 2]	S55004	970	329	*
[Ref.: #20218]	INSDC Sequence	16S rRNA [Burkholderia pickettii, ATCC 27511, Genomic, 377 nt, segment 2 of 2]	S55018	377	329	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Ralstonia pickettii strain BCRC 14820 16S-23S ribosomal RNA intergenic spacer, complete sequence	EU014524	515	329	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Ralstonia pickettii strain CIP 7323 16S ribosomal RNA gene, partial sequence	EU024148	1316	329	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Ralstonia pickettii strain CIP 7323 DNA gyrase subunit beta (gyrB) gene, partial cds	EU024192	432	329	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Ralstonia pickettii strain CIP 7323 RNA polymerase beta subunit (rpoB) gene, partial cds	EU024285	708	329	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Ralstonia pickettii strain LMG5942 flagellin (fliC) gene, partial cds	AY192716	724	329	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Ralstonia pickettii strain DSM6297 flagellin (fliC) gene, partial cds	AY192717	724	329	*
[Ref.: #20218]	Marker Gene (DDBJ Direct submission)	Ralstonia pickettii gene for 16S rRNA, partial sequence, strain: JCM 5969	AB626131	1460	329	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Ralstonia pickettii 16S rRNA gene, ITS1 and 23S rRNA gene, strain JCM5969	AM501933	827	329	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Ralstonia pickettii partial fliC gene for flagellin, strain JCM5969	FN869041	472	329	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Ralstonia pickettii (strain LMC 5942 T) 5S ribosomal RNA	AJ131600	115	329	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Ralstonia pickettii 16S rRNA gene, 23S rRNA gene and ITS1, specimen voucher LMG5942	AJ783972	888	329	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Ralstonia pickettii strain LMG5942 50S ribosomal subunit protein L2 (rplB) gene, partial cds	JF702205	654	329	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Ralstonia pickettii strain LMG5942 adenylate kinase (adk) gene, partial cds	JF702297	468	329	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Ralstonia pickettii strain LMG5942 flagellar filament structural protein (fliC) gene, partial cds	JF702409	348	329	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Ralstonia pickettii strain LMG5942 NADP-specific glutamate dehydrogenase (gdhA) gene, partial cds	JF702502	966	329	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Ralstonia pickettii strain LMG5942 DNA gyrase subunit B (gyrB) gene, partial cds	JF702595	423	329	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Ralstonia pickettii strain LMG5942 leucyl-tRNA synthetase (leuS) gene, partial cds	JF702687	735	329	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Ralstonia pickettii strain LMG5942 methyl-directed DNA mismatch repair protein (mutS) gene, partial cds	JF702733	651	329	*
[Ref.: #20218]	Marker Gene (DDBJ Direct submission)	Ralstonia pickettii gene for 16S rRNA, partial sequence, strain: NBRC 102503	AB681837	1463	329	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Ralstonia pickettii strain NCTC 11149 DNA gyrase subunit B (gyrB) gene, partial cds	JQ914279	815	329	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	P.pickettii (group 2) 16S rRNA subunit	X70349	294	329	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Ralstonia pickettii partial 16S rRNA gene, strain PSE055, ATCC 27512	AJ270265	474	329	*
[Ref.: #2581]	INSDC	Ralstonia pickettii partial 16S rRNA gene, type strain DSM 6297T	LN681565	1490	329

Availability in culture collectionsStrain availability

[Ref.: #2581]

Culture collection no.

DSM 6297, ATCC 27511, CIP 73.23, JCM 5969, NCTC 11149

[Ref.: #2581]

Strain history

<- CIP <- F. Pichinoty <- M.J. Picketti; K-288

[Ref.: #20218]

Associated Passport(s) in StrainInfo

154453, 17899, 234599, 17904, 17906

References

#2581

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6297

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#22943

P. Vandamme, J. Goris, T. Coenye, B. Hoste, D. Janssens, K. Kersters, P. De Vos, E. Falsen: Assignment of Centers for Disease Control group IVc-2 to the genus Ralstonia as Ralstonia paucula sp. nov.. IJSEM 49: 663 - 669 1999 ( DOI 10.1099/00207713-49-2-663 , PubMed 10319489 )

#22944

T De Baere, S Steyaert, G Wauters, P Des Vos, J Goris, T Coenye, T Suyama, G Verschraegen, M Vaneechoutte: Classification of Ralstonia pickettii biovar 3/'thomasii' strains (Pickett 1994) and of new isolates related to nosocomial recurrent meningitis as Ralstonia mannitolytica sp. nov.. IJSEM 51: 547 - 558 2001 ( DOI 10.1099/00207713-51-2-547 , PubMed 11321101 )

#36535 ; Curators of the CIP;
* These References are textmined

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