Virgibacillus arcticus Hal1 | Type strain | DSM 19574, JCM 14839

Strain identifier

BacDive ID: 1487

Type strain:

Species: Virgibacillus arcticus

Strain Designation: Hal1

Culture col. no.: DSM 19574, JCM 14839

Section

Name and taxonomic classification
Morphology and physiology
Culture and growth conditions
Isolation, sampling and environmental information
Application and interaction
Molecular biology
Strain availability

External links

Information on the name and the taxonomic classification.Name and taxonomic classification

[Ref.: #8188]	Domain	Bacteria
[Ref.: #8188]	Phylum	Firmicutes
[Ref.: #8188]	Class	Bacilli
[Ref.: #8188]	Order	Bacillales
[Ref.: #8188]	Family	Bacillaceae
[Ref.: #8188]	Genus	Virgibacillus
[Ref.: #8188]	Species	Virgibacillus arcticus
[Ref.: #8188]	Full Scientific Name	Virgibacillus arcticus Niederberger et al. 2009
[Ref.: #8188]	Strain Designation	Hal1
[Ref.: #8188]	Type strain

		Prokaryotic Nomenclature Up-to-date (PNU)	Taxonomical status	Literature reference
[Ref.: #20215]	Domain	Bacteria
[Ref.: #20215]	Phylum	Firmicutes
[Ref.: #20215]	Class	Bacilli		Int. J. Syst. Evol. Microbiol. 60:469
[Ref.: #20215]	Family	Bacillaceae		Int. J. Syst. Bacteriol. 30:235
[Ref.: #20215]	Genus	Virgibacillus	gen. nov. (VP)	Int. J. Syst. Bacteriol. 48:104*
[Ref.: #20215]	Species	Virgibacillus arcticus	sp. nov. (VP)	Int. J. Syst. Evol. Microbiol. 59:2223*
[Ref.: #20215]	Full Scientific Name	Virgibacillus arcticus Niederberger et al. 2009

Information on morphological and physiological propertiesMorphology and physiology

[Ref.: #23057]	Gram stain	positive
[Ref.: #23057]	Cell length	2.0-5.0 µm
[Ref.: #23057]	Cell width	0.5-0.8 µm
[Ref.: #23057]	Cell shape	rod-shaped
[Ref.: #23057]	Motility	yes

[Ref.: #23057]	Colony size	2.0 mm
[Ref.: #23057]	Incubation period	7 days
[Ref.: #23057]	Cultivation medium used	1/4 HM plates

	Enzyme	Enzyme activity	EC number
[Ref.: #23057]	alkaline phosphatase	-	3.1.3.1
[Ref.: #23057]	arginine dihydrolase	-	3.5.3.6
[Ref.: #23057]	beta-galactosidase	-	3.2.1.23
[Ref.: #23057]	catalase	+	1.11.1.6
[Ref.: #23057]	cytochrome oxidase	-	1.9.3.1
[Ref.: #23057]	gelatinase	+
[Ref.: #23057]	lysine decarboxylase	-	4.1.1.18
[Ref.: #23057]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #23057]	tryptophan deaminase	-	4.1.99.1
[Ref.: #23057]	urease	-	3.5.1.5

[Ref.: #23057]

Halophily / tolerance level

moderately halophilic

	Salt	Tested relation		Salt conc.
[Ref.: #23057]	NaCl		growth	0.0-20.0	%(w/v)
[Ref.: #23057]	NaCl		optimum	5.0	%(w/v)
[Ref.: #23057]	NaCl	no	growth	25.0	%

	Metabolite	Antibiotic sensitivity
[Ref.: #23057]	Ampicillin	yes
[Ref.: #23057]	Chloramphenicol	yes
[Ref.: #23057]	Erythromycin	yes
[Ref.: #23057]	Rifampicin	yes
[Ref.: #23057]	Streptomycin	yes

	Metabolite	Production
[Ref.: #23057]	Acetoin	no
[Ref.: #23057]	Hydrogen sulfide	no
[Ref.: #23057]	Indole	no

	Metabolite	Voges-Proskauer-test	Indole test	Citrate test
[Ref.: #23057]	Acetoin	-
[Ref.: #23057]	Citric acid			-
[Ref.: #23057]	Indole		-

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23057]	Amygdalin	-	builds acid from
[Ref.: #23057]	Casein	+	hydrolysis
[Ref.: #23057]	Cellobiose	-	carbon source
[Ref.: #23057]	Cellobiose	-	growth
[Ref.: #23057]	Citric acid	-	carbon source
[Ref.: #23057]	D-Fructose	+	builds acid from
[Ref.: #23057]	D-Glucose	+	builds acid from
[Ref.: #23057]	D-Glucose	+	growth
[Ref.: #23057]	D-Lactose	-	carbon source
[Ref.: #23057]	D-Lactose	-	growth
[Ref.: #23057]	D-Mannitol	+	builds acid from
[Ref.: #23057]	D-Mannose	+	builds acid from
[Ref.: #23057]	D-Sorbitol	-	builds acid from
[Ref.: #23057]	Gelatin	+	hydrolysis
[Ref.: #23057]	Inositol	-	builds acid from
[Ref.: #23057]	L-Arabinose	-	builds acid from
[Ref.: #23057]	L-Rhamnose	-	builds acid from
[Ref.: #23057]	Lactose	+	builds acid from
[Ref.: #23057]	Maltose	+	builds acid from
[Ref.: #23057]	Melibiose	-	carbon source
[Ref.: #23057]	Melibiose	-	growth
[Ref.: #23057]	Melibiose	+	builds acid from
[Ref.: #23057]	Methyl alpha-D-glucopyranoside	+	builds acid from
[Ref.: #23057]	Nitrate	+	reduction
[Ref.: #23057]	Raffinose	-	builds acid from
[Ref.: #23057]	Sucrose	-	builds acid from
[Ref.: #23057]	Sucrose	+	growth
[Ref.: #23057]	Trehalose	-	carbon source
[Ref.: #23057]	Trehalose	-	growth
[Ref.: #23057]	Trehalose	+	builds acid from
[Ref.: #23057]	Tryptophan	-	energy source
[Ref.: #23057]	Xylitol	+	builds acid from
[Ref.: #23057]	Xylose	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

[Ref.: #8188]	Murein short key	A11
[Ref.: #8188]	Murein types	A1alpha L-Lys-direct
[Ref.: #23057]	Murein short key	A11
[Ref.: #23057]	Murein types	A1alpha L-Lys-direct

[Ref.: #23057]

Pigmentation color

light pink

[Ref.: #23057]	Spore description	ellipsoidal,central or subterminal
[Ref.: #23057]	Type of spore	endospore
[Ref.: #23057]	Ability of spore formation	yes

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #8188]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514), 20°C

[Ref.: #8188]

Culture medium growth

[Ref.: #8188]

Culture medium link

medium recipe provided by DSMZ

[Ref.: #23057]

Culture medium

1/4 HM plates

[Ref.: #23057]

Culture medium growth

	Kind of temperature		Temperature
[Ref.: #8188]		growth	20 ̊C
[Ref.: #23057]		growth	0.0-30.0 ̊C
[Ref.: #23057]	no	growth	-5.0 ̊C
[Ref.: #23057]	no	growth	37.0 ̊C
[Ref.: #23057]		optimum	25.0 ̊C

[Ref.: #8188]	Temperature range	psychrophilic
[Ref.: #23057]	Temperature range	psychrophilic

	Kind of pH		pH
[Ref.: #23057]		optimum	7.0
[Ref.: #23057]		growth	4.4-9.1
[Ref.: #23057]	no	growth	10.0

Information on isolation source, the sampling and environmental conditionsIsolation, sampling and environmental information

[Ref.: #8188]

Sample type/isolated from

permafrost core of 9 m depth

[Ref.: #8188]

Geographic location (country and/or sea, region)

Canadian high Arctic, Ellesmere Island, Eureka

[Ref.: #8188]

Country

Canada

[Ref.: #8188]

Continent

North America

[Ref.: #23057]

Enrichment culture

1/4 HM medium

[Ref.: #23057]

Enrichment culture composition

per litre: 178 g NaCl, 2 g KCl, 1 g MgSO4.7H2O, 0.36 g CaCl2.2H2O, 0.23 g NaBr, 0.06 g NaHCO3 , 0.01 g FeCl3 , 1.25 g proteose-peptone, 2.5 g yeast extract and 0.25 g glucose, adjusted to about pH 7.0

[Ref.: #23057]

Enrichment culture duration

14 days

[Ref.: #23057]

Enrichment culture temperature

23.0 ̊C

[Ref.: #23057]

Enrichment culture

1/4 HM medium

[Ref.: #23057]

Enrichment culture composition

[Ref.: #23057]

Enrichment culture duration

30 days

[Ref.: #23057]

Enrichment culture temperature

5.0 ̊C

Isolation sources categories

#Environmental	#Terrestrial	#Core sample
#Environmental	#Terrestrial	#Permafrost

What are isolation sources categories?

* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hostsApplication and interaction

[Ref.: #8188]

Biosafety level

Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databassMolecular biology

[Ref.: #8188]	GC-content	38.2 mol%	fluorimetric
[Ref.: #23057]	GC-content	38.2 mol%	fluorimetric

Sequence information:

	Sequence database	Sequence accession description	Sequence accession number	Sequence length (bp)	Associated NCBI tax ID
[Ref.: #8188]	GenBank Direct submission	Virgibacillus arcticus strain Hal 1 16S ribosomal RNA gene, partial sequence	EF675742	1350	469869

Availability in culture collectionsStrain availability

[Ref.: #8188]

Culture collection no.

DSM 19574, JCM 14839

[Ref.: #8188]

Strain history

<- T. D. Niederberger and L.G. Whyte, Department of Natural Resource Sciences, McGill University, Canada <- B. Steven; Hal1

[Ref.: #20218]

Associated Passport(s) in StrainInfo

844795, 844796

References

#8188

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19574

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23057

Thomas D. Niederberger,Blaire Steven,Sophie Charvet,Beatrice Barbier,Lyle G. Whyte: Virgibacillus arcticus sp. nov., a moderately halophilic, endospore-forming bacterium from permafrost in the Canadian high Arctic. IJSEM 59: 2219 - 2225 2009 ( DOI 10.1099/ijs.0.002931-0 )

* These References are textmined

Search for species Virgibacillus arcticus in external resources:
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