Gracilibacillus halotolerans NN | Type strain | DSM 11805

Strain identifier

BacDive ID: 1460

Type strain:

Species: Gracilibacillus halotolerans

Strain Designation: NN

Culture col. no.: DSM 11805

Section

Name and taxonomic classification
Morphology and physiology
Culture and growth conditions
Isolation, sampling and environmental information
Application and interaction
Molecular biology
Strain availability

External links

Information on the name and the taxonomic classification.Name and taxonomic classification

[Ref.: #4494]	Domain	Bacteria
[Ref.: #4494]	Phylum	Firmicutes
[Ref.: #4494]	Class	Bacilli
[Ref.: #4494]	Order	Bacillales
[Ref.: #4494]	Family	Bacillaceae
[Ref.: #4494]	Genus	Gracilibacillus
[Ref.: #4494]	Species	Gracilibacillus halotolerans
[Ref.: #4494]	Full Scientific Name	Gracilibacillus halotolerans Wainoe et al. 1999
[Ref.: #4494]	Strain Designation	NN
[Ref.: #4494]	Type strain

		Prokaryotic Nomenclature Up-to-date (PNU)	Taxonomical status	Literature reference
[Ref.: #20215]	Domain	Bacteria
[Ref.: #20215]	Phylum	Firmicutes
[Ref.: #20215]	Class	Bacilli		Int. J. Syst. Evol. Microbiol. 60:469
[Ref.: #20215]	Family	Bacillaceae		Int. J. Syst. Bacteriol. 30:235
[Ref.: #20215]	Genus	Gracilibacillus	gen. nov. (VP)	Int. J. Syst. Bacteriol. 49:829*
[Ref.: #20215]	Species	Gracilibacillus halotolerans	sp. nov. (VP)	Int. J. Syst. Bacteriol. 49:829*
[Ref.: #20215]	Full Scientific Name	Gracilibacillus halotolerans Wainoe et al. 1999

Information on morphological and physiological propertiesMorphology and physiology

[Ref.: #23041]	Gram stain	positive
[Ref.: #23041]	Cell length	2.0-5.0 µm
[Ref.: #23041]	Cell width	0.4-0.6 µm
[Ref.: #23041]	Cell shape	rod-shaped
[Ref.: #23041]	Motility	yes
[Ref.: #23041]	Flagellum arrangement	peritrichous

[Ref.: #23041]	Colony size	2.0 mm
[Ref.: #23041]	Colony color	creamy white
[Ref.: #23041]	Colony shape	circular
[Ref.: #23041]	Incubation period	5 days
[Ref.: #23041]	Cultivation medium used	GSL-2 agar medium

	Enzyme	Enzyme activity	EC number
[Ref.: #23041]	alkaline phosphatase	+	3.1.3.1
[Ref.: #23041]	arginine dihydrolase	-	3.5.3.6
[Ref.: #23041]	beta-galactosidase	+	3.2.1.23
[Ref.: #23041]	catalase	+	1.11.1.6
[Ref.: #23041]	chitinase	-	3.2.1.14
[Ref.: #23041]	cytochrome oxidase	+	1.9.3.1
[Ref.: #23041]	lecithinase	-
[Ref.: #23041]	lysine decarboxylase	-	4.1.1.18
[Ref.: #23041]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #23041]	phenylalanine deaminase	-	4.3.1.5

[Ref.: #23041]

Halophily / tolerance level

halotolerant

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23041]		NaCl		growth	0.0-20.0	%(w/v)
[Ref.: #23041]		NaCl		optimum	0.0	%

	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #23041]	Ampicillin		yes
[Ref.: #23041]	Bacitracin	yes
[Ref.: #23041]	Carbenicillin	yes
[Ref.: #23041]	Chloramphenicol	yes
[Ref.: #23041]	Erythromycin	yes
[Ref.: #23041]	Gentamicin		yes
[Ref.: #23041]	Kanamycin		yes
[Ref.: #23041]	Nalidixic acid		yes
[Ref.: #23041]	Neomycin		yes
[Ref.: #23041]	Novobiocin	yes
[Ref.: #23041]	Penicillin	yes
[Ref.: #23041]	Rifampicin	yes
[Ref.: #23041]	Tetracycline		yes
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #23041]	Acetoin	no
[Ref.: #23041]	Hydrogen sulfide	yes
[Ref.: #23041]	Indole	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #23041]		Acetoin	-

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23041]	Acetamide	-	growth
[Ref.: #23041]	Acetic acid	-	growth
[Ref.: #23041]	Acetone	-	growth
[Ref.: #23041]	Adipic acid	-	growth
[Ref.: #23041]	Amylose	+	growth
[Ref.: #23041]	Anisic acid	-	growth
[Ref.: #23041]	Arabinose	+	growth
[Ref.: #23041]	Ascorbate	+	growth
[Ref.: #23041]	Aspartic acid	-	growth
[Ref.: #23041]	Benzamide	-	growth
[Ref.: #23041]	Benzoate	-	growth
[Ref.: #23041]	Betaine	-	growth
[Ref.: #23041]	Butanol	-	growth
[Ref.: #23041]	Butyrate	-	growth
[Ref.: #23041]	Caprylate	-	growth
[Ref.: #23041]	Casein	-	hydrolysis
[Ref.: #23041]	Cellulose	-	growth
[Ref.: #23041]	Citric acid	-	growth
[Ref.: #23041]	Coconut oil	-	growth
[Ref.: #23041]	Crab shell chitin	-	growth
[Ref.: #23041]	D-Cellobiose	+	growth
[Ref.: #23041]	D-Fructose	+	growth
[Ref.: #23041]	D-Galactose	+	growth
[Ref.: #23041]	D-Galacturonic acid	+	growth
[Ref.: #23041]	D-Gluconic acid	+	growth
[Ref.: #23041]	D-Glucose	+	builds acid from
[Ref.: #23041]	D-Glucuronic acid	+	growth
[Ref.: #23041]	D-Mannitol	+	builds acid from
[Ref.: #23041]	D-Mannose	+	growth
[Ref.: #23041]	D-Melezitose	+	growth
[Ref.: #23041]	D-Melibiose	+	growth
[Ref.: #23041]	D-Sorbitol	-	growth
[Ref.: #23041]	D-Trehalose	+	growth
[Ref.: #23041]	D-Xylose	+	builds acid from
[Ref.: #23041]	D-Xylose	+	growth
[Ref.: #23041]	Esculin	+	hydrolysis
[Ref.: #23041]	Ethanol	-	growth
[Ref.: #23041]	Ethanolamine	-	growth
[Ref.: #23041]	Formic acid	-	growth
[Ref.: #23041]	Fucose	-	growth
[Ref.: #23041]	Fumaric acid	-	growth
[Ref.: #23041]	Gelatin	+	hydrolysis
[Ref.: #23041]	Glucose	-	fermentation
[Ref.: #23041]	Glucose	+	growth
[Ref.: #23041]	Glutaric acid	-	growth
[Ref.: #23041]	Glycerol	+	growth
[Ref.: #23041]	Glycogen	+	growth
[Ref.: #23041]	Glycolate	-	growth
[Ref.: #23041]	Glyoxylate	-	growth
[Ref.: #23041]	Hexanoic acid	-	growth
[Ref.: #23041]	Inulin	+	growth
[Ref.: #23041]	L-Alanine	-	growth
[Ref.: #23041]	L-Arginine	-	growth
[Ref.: #23041]	L-Cysteine	-	growth
[Ref.: #23041]	L-Glutamic acid	-	growth
[Ref.: #23041]	L-Lysine	-	growth
[Ref.: #23041]	L-Malate	+	growth
[Ref.: #23041]	L-Methionine	-	growth
[Ref.: #23041]	L-Ornithine	-	growth
[Ref.: #23041]	L-Phenylalanine	-	growth
[Ref.: #23041]	L-Proline	-	growth
[Ref.: #23041]	L-Rhamnose	+	growth
[Ref.: #23041]	L-Serine	-	growth
[Ref.: #23041]	L-Threonine	-	growth
[Ref.: #23041]	Lactic acid	-	growth
[Ref.: #23041]	Lactose	+	growth
[Ref.: #23041]	Maltose	+	growth
[Ref.: #23041]	Mannitol	+	growth
[Ref.: #23041]	Methanol	-	growth
[Ref.: #23041]	Methylamine	-	growth
[Ref.: #23041]	N-Acetylglucosamine	+	growth
[Ref.: #23041]	Nicotinate	-	growth
[Ref.: #23041]	Nitrate	+	reduction
[Ref.: #23041]	Oil of cedar wood	-	growth
[Ref.: #23041]	Oxoglutaric acid	+	growth
[Ref.: #23041]	Pentanol	-	growth
[Ref.: #23041]	Picolinic acid	-	growth
[Ref.: #23041]	Propanol	-	growth
[Ref.: #23041]	Propionic acid	-	growth
[Ref.: #23041]	Pyruvic acid	-	growth
[Ref.: #23041]	Raffinose	+	growth
[Ref.: #23041]	Starch	+	growth
[Ref.: #23041]	Starch	+	hydrolysis
[Ref.: #23041]	Succinic acid	-	growth
[Ref.: #23041]	Sucrose	+	growth
[Ref.: #23041]	Sulfanilamide	-	growth
[Ref.: #23041]	Trimethylamine	+	growth
[Ref.: #23041]	Tryptophan	-	growth
[Ref.: #23041]	Tween 80	+	growth
[Ref.: #23041]	Tween 80	+	hydrolysis
[Ref.: #23041]	Urea	+	hydrolysis
[Ref.: #23041]	Valeric acid	-	growth
[Ref.: #23041]	Xylose	-	fermentation
[Ref.: #23041]	Yeast extract	+	required for growth
	Only first 10 entries are displayed. Click here to see all.

[Ref.: #23041]	Murein short key	A31
[Ref.: #23041]	Murein types	A1gamma m-Dpm-direct

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Nutrition type

chemoorganotroph

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Oxygen tolerance

obligate aerobe

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Pigmentation color

creamy white

[Ref.: #23041]	Spore description	ellipsoid,terminal,in swollen sporangia
[Ref.: #23041]	Type of spore	endospore
[Ref.: #23041]	Ability of spore formation	yes

Information on culture and growth conditionsCulture and growth conditions

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Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514), 30°C

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Culture medium growth

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Culture medium link

medium recipe provided by DSMZ

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Culture medium

GRACILIBACILLUS MEDIUM (DSMZ Medium 802), 30°C

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Culture medium growth

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Culture medium link

medium recipe provided by DSMZ

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Culture medium

GSL-2 agar medium

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Culture medium growth

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Culture medium

Tris-medium

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Culture medium growth

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #4494]	growth	30 ̊C
[Ref.: #23041]	growth	6.0-50.0 ̊C
[Ref.: #23041]	optimum	47.0 ̊C

[Ref.: #4494]	Temperature range	mesophilic
[Ref.: #23041]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #23041]			optimum	7.5
[Ref.: #23041]			growth	5.0-10.0

Information on isolation source, the sampling and environmental conditionsIsolation, sampling and environmental information

[Ref.: #23041]

Sample type/isolated from

surface mud

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Geographic location (country and/or sea, region)

Great Salt Lake,Utah

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Country

USA

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Continent

North America

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Enrichment culture

liquid GSL-2 medium

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Enrichment culture composition

containing of (1-1 distilled water): NaCl, 100g; MgSO4.7H2O, 10g; KCl, 5g; NH4Cl, 2g; NaHCO3, 1g; citric acid, 0.5g; yeast extract (Difco), 2g; trypticase peptone (BBL), 2g; trace metal solution (TMS 3), 2ml; KH2PO4 (50 g l-1),10 ml; CaCl2.2H2O (20 g 1-l),10;FeCl2/MnCl2 solution [FeCl2.4H2O (20 gl-1) + MnCl2.4H2O (20 gl-1)], 2ml and Glucose (0.2% w/v)

Isolation sources categories

#Environmental

#Terrestrial

#Mud (sludge)

What are isolation sources categories?

* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hostsApplication and interaction

[Ref.: #4494]

Biosafety level

Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databassMolecular biology

[Ref.: #23041]

GC-content

38 mol%

Sequence information:

	Sequence database	Sequence accession description	Sequence accession number	Sequence length (bp)	Associated NCBI tax ID
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Gracilibacillus halotolerans isolate Gh10 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723657	439	74386	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Gracilibacillus halotolerans isolate Gh19 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723658	592	74386	*
[Ref.: #4494]	GenBank Direct submission		AF036922

Availability in culture collectionsStrain availability

[Ref.: #4494]

Culture collection no.

DSM 11805

[Ref.: #4494]

Strain history

<- K. Ingvorsen, NN

[Ref.: #20218]

Associated Passport(s) in StrainInfo

118588

References

#4494

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11805

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23041

Michael Wain?,B. J. Tindall,Peter Schumann,Kjeld Ingvorsen: Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov.. IJSEM 49: 821 - 831 1999 ( DOI 10.1099/00207713-49-2-821 )

* These References are textmined

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