Strain identifier
BacDive ID: 4933
Type strain:
Species: Ewingella americana
Strain Designation: 1468-78
Strain history: CIP <- 1981, PAD Grimont, Inst. Pasteur, Paris, France: strain 1468-78 <- Kentucky State Hlth. Dept., USA
NCBI tax ID(s): 910964 (strain), 41202 (species)
General
@ref: 1778
BacDive-ID: 4933
DSM-Number: 4580
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Ewingella americana 1468-78 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from throat.
NCBI tax id
NCBI tax id | Matching level |
---|---|
41202 | species |
910964 | strain |
strain history
@ref | history |
---|---|
1778 | <- CIP <- P.A.D. Grimont <- CDC, 1468-78 |
67770 | CIP 81.94 <-- P. A. D. Grimont; Inst. Pasteur, France; 1468-78 <-- Kentucky State Health Dept., USA. |
116326 | CIP <- 1981, PAD Grimont, Inst. Pasteur, Paris, France: strain 1468-78 <- Kentucky State Hlth. Dept., USA |
doi: 10.13145/bacdive4933.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Enterobacterales
- family: Yersiniaceae
- genus: Ewingella
- species: Ewingella americana
- full scientific name: Ewingella americana Grimont et al. 1984
@ref: 1778
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Enterobacteriales, not validated
family: Yersiniaceae
genus: Ewingella
species: Ewingella americana
full scientific name: Ewingella americana Grimont et al. 1984
strain designation: 1468-78
type strain: yes
Morphology
cell morphology
- @ref: 116326
- gram stain: negative
- cell shape: rod-shaped
- motility: yes
Culture and growth conditions
culture medium
@ref | name | growth | composition | link |
---|---|---|---|---|
37628 | MEDIUM 3 - Columbia agar | yes | Columbia agar (39.000 g);distilled water (1000.000 ml) | |
1778 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium92.pdf | |
1778 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium693.pdf | |
116326 | CIP Medium 3 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3 | |
116326 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
1778 | positive | growth | 30 | mesophilic |
37628 | positive | growth | 30 | mesophilic |
46118 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
46118 | aerobe |
116326 | facultative anaerobe |
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 5-ketogluconate | + | builds acid from | |
68371 | Potassium 2-ketogluconate | + | builds acid from | |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | + | builds acid from | 18333 |
68371 | L-fucose | - | builds acid from | 18287 |
68371 | D-fucose | - | builds acid from | 28847 |
68371 | D-tagatose | - | builds acid from | 16443 |
68371 | D-lyxose | - | builds acid from | 62318 |
68371 | turanose | - | builds acid from | 32528 |
68371 | gentiobiose | + | builds acid from | 28066 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | glycogen | - | builds acid from | 28087 |
68371 | starch | - | builds acid from | 28017 |
68371 | raffinose | - | builds acid from | 16634 |
68371 | melezitose | - | builds acid from | 6731 |
68371 | inulin | - | builds acid from | 15443 |
68371 | trehalose | + | builds acid from | 27082 |
68371 | sucrose | - | builds acid from | 17992 |
68371 | melibiose | - | builds acid from | 28053 |
68371 | lactose | + | builds acid from | 17716 |
68371 | maltose | + | builds acid from | 17306 |
68371 | cellobiose | + | builds acid from | 17057 |
68371 | salicin | + | builds acid from | 17814 |
68371 | esculin | + | builds acid from | 4853 |
68371 | arbutin | + | builds acid from | 18305 |
68371 | amygdalin | - | builds acid from | 27613 |
68371 | N-acetylglucosamine | + | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | - | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | - | builds acid from | 17924 |
68371 | D-mannitol | + | builds acid from | 16899 |
68371 | myo-inositol | - | builds acid from | 17268 |
68371 | galactitol | - | builds acid from | 16813 |
68371 | L-rhamnose | - | builds acid from | 62345 |
68371 | L-sorbose | - | builds acid from | 17266 |
68371 | D-mannose | + | builds acid from | 16024 |
68371 | D-fructose | + | builds acid from | 15824 |
68371 | D-glucose | + | builds acid from | 17634 |
68371 | D-galactose | + | builds acid from | 12936 |
68371 | methyl beta-D-xylopyranoside | - | builds acid from | 74863 |
68371 | ribitol | - | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | D-xylose | - | builds acid from | 65327 |
68371 | D-ribose | + | builds acid from | 16988 |
68371 | L-arabinose | - | builds acid from | 30849 |
68371 | D-arabinose | - | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
68371 | glycerol | + | builds acid from | 17754 |
116326 | nitrate | + | reduction | 17632 |
116326 | nitrite | - | reduction | 16301 |
metabolite production
- @ref: 116326
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | + | 3.1.3.1 |
116326 | oxidase | - | |
116326 | catalase | + | 1.11.1.6 |
116326 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
116326 | - | + | + | + | - | + | - | - | - | - | + | + | - | - | - | - | - | + | - | - |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
116326 | + | - | - | - | + | - | - | - | - | + | + | + | + | - | - | - | - | + | - | - | - | + | - | + | + | + | + | + | + | - | - | + | - | - | - | - | - | - | + | - | - | - | - | - | + | - | +/- | + | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | geographic location | isolation date |
---|---|---|---|---|---|---|
1778 | throat | |||||
46118 | Human throat | |||||
67770 | Human throat | USA | USA | North America | ||
116326 | Human, Throat | United States of America | USA | North America | Kentucky | 1978 |
isolation source categories
- Cat1: #Host Body-Site
- Cat2: #Oral cavity and airways
- Cat3: #Throat
taxonmaps
- @ref: 69479
- File name: preview.99_7661.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_106;97_324;98_5604;99_7661&stattab=map
- Last taxonomy: Yersiniaceae
- 16S sequence: JN175329
- Sequence Identity:
- Total samples: 805
- soil counts: 42
- aquatic counts: 108
- animal counts: 420
- plant counts: 235
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
1778 | 2 | Risk group (German classification) |
116326 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Ewingella americana strain CIP 81.94 16S ribosomal RNA gene, complete sequence | JN175329 | 1534 | ena | 41202 |
67770 | Ewingella americana gene for 16S rRNA, partial sequence, strain: GTC 1277 | AB273745 | 1489 | ena | 41202 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Ewingella americana NCTC12157 | GCA_900451015 | contig | ncbi | 41202 |
66792 | Ewingella americana ATCC 33852 | 910964.7 | wgs | patric | 910964 |
66792 | Ewingella americana strain NCTC12157 | 41202.9 | wgs | patric | 41202 |
66792 | Ewingella americana C.024, ATCC 33852 | 2588253786 | draft | img | 910964 |
67770 | Ewingella americana ATCC 33852 | GCA_000735345 | contig | ncbi | 910964 |
67770 | Ewingella americana CCUG 14506T | GCA_008693045 | contig | ncbi | 41202 |
GC content
- @ref: 67770
- GC-content: 53.6-55.2
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | yes | 91.035 | no |
flagellated | no | 60.901 | no |
gram-positive | no | 98.123 | no |
anaerobic | no | 98.726 | yes |
halophile | no | 89.935 | no |
spore-forming | no | 96.353 | no |
thermophile | no | 99.688 | yes |
glucose-util | yes | 95.627 | no |
aerobic | yes | 72.937 | no |
glucose-ferment | yes | 91.105 | no |
External links
@ref: 1778
culture collection no.: CCUG 14506, NCTC 12157, LMG 7869, CECT 859, CCM 4555, JCM 5911, DSM 4580, ATCC 33852, CIP 81.94, NRRL B-24919, CDC 1468-78, JCM 32812, GTC 1277, ICMP 15665
straininfo link
- @ref: 74430
- straininfo: 13669
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 6847036 | Ewingella americana gen.nov., sp.nov., a new Enterobacteriaceae isolated from clinical specimens. | Grimont PA, Farmer JJ 3rd, Grimont F, Asbury MA, Brenner DJ, Deval C | Ann Microbiol (Paris) | 10.1016/0769-2609(83)90102-3 | 1983 | Base Composition, DNA, Bacterial, Enterobacteriaceae/classification/genetics/*isolation & purification, Genotype, Humans, Nucleic Acid Hybridization, Phenotype, Terminology as Topic | Genetics |
Pathogenicity | 14598935 | Natural antibiotic susceptibility of Ewingella americana strains. | Stock I, Sherwood KJ, Wiedemann B | J Chemother | 10.1179/joc.2003.15.5.428 | 2003 | Databases, Factual, *Drug Resistance, Bacterial, Enterobacteriaceae/*drug effects/*pathogenicity, Enterobacteriaceae Infections/*drug therapy, Microbial Sensitivity Tests |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
1778 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4580) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-4580 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
37628 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/11261 | ||||
46118 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 14506) | https://www.ccug.se/strain?id=14506 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68371 | Automatically annotated from API 50CH acid | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
74430 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID13669.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
116326 | Curators of the CIP | Collection of Institut Pasteur (CIP 81.94) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2081.94 |