Strain identifier

BacDive ID: 2796

Type strain: Yes

Species: Clostridium uliginosum

Strain Designation: CK55

Strain history: <- C. Matthies and C. H. Kuhner, Universität Bayreuth, Bayreuth, Germany; CK55 <- C. H. Kuhner {1994}

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 4870

BacDive-ID: 2796

DSM-Number: 12992

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, psychrophilic, motile

description: Clostridium uliginosum CK55 is an anaerobe, spore-forming, psychrophilic bacterium that was isolated from acidic peat bog.

NCBI tax id

NCBI tax idMatching level
1121343strain
119641species

strain history

  • @ref: 4870
  • history: <- C. Matthies and C. H. Kuhner, Universität Bayreuth, Bayreuth, Germany; CK55 <- C. H. Kuhner {1994}

doi: 10.13145/bacdive2796.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium uliginosum
  • full scientific name: Clostridium uliginosum Matthies et al. 2001

@ref: 4870

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Clostridium

species: Clostridium uliginosum

full scientific name: Clostridium uliginosum Matthies et al. 2001

strain designation: CK55

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480yes94.554
69480100positive

Culture and growth conditions

culture medium

  • @ref: 4870
  • name: ANAEROBIC TRYPTIC SOY BROTH MEDIUM (DSMZ Medium 869)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/869
  • composition: Name: ANAEROBIC TRYPTIC SOY BROTH MEDIUM (DSMZ Medium 869) Composition: Trypticase soy broth 5.5 g/l D-Glucose 2.0 g/l Resazurin 0.0005 g/l Distilled water

culture temp

  • @ref: 4870
  • growth: positive
  • type: growth
  • temperature: 20
  • range: psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
4870anaerobe
69480anaerobe99.999

spore formation

@refspore formationconfidence
69481yes100
69480yes100

Isolation, sampling and environmental information

isolation

  • @ref: 4870
  • sample type: acidic peat bog
  • geographic location: East-central Germany, Fichtelgebirge near Bayreuth
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Wetland (Swamp)
#Condition#Acidic

Safety information

risk assessment

  • @ref: 4870
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 4870
  • description: Clostridium uliginosum partial 16S rRNA gene, strain CK55
  • accession: AJ276992
  • length: 1437
  • database: ena
  • NCBI tax ID: 119641

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Clostridium uliginosum DSM 12992GCA_900112485scaffoldncbi119641
66792Clostridium uliginosum strain DSM 12992119641.3wgspatric119641
66792Clostridium uliginosum DSM 129922675903229draftimg119641

GC content

  • @ref: 4870
  • GC-content: 28
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes82.036no
flagellatedyes53.509no
gram-positiveyes74.714no
anaerobicyes98.91yes
aerobicno98.223no
halophileno93.88no
spore-formingyes94.515no
glucose-fermentyes75.913no
thermophileno99.33yes
glucose-utilyes90.296no

External links

@ref: 4870

culture collection no.: DSM 12992, ATCC BAA 53

straininfo link

  • @ref: 72322
  • straininfo: 49385

literature

  • topic: Phylogeny
  • Pubmed-ID: 11411680
  • title: Clostridium uliginosum sp. nov., a novel acid-tolerant, anaerobic bacterium with connecting filaments.
  • authors: Matthies C, Kuhner CH, Acker G, Drake HL
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-51-3-1119
  • year: 2001
  • mesh: Anaerobiosis, Base Sequence, Carbohydrate Metabolism, Clostridium/*classification/genetics/physiology/ultrastructure, DNA, Ribosomal/genetics, Enzymes/analysis, Microscopy, Electron, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Soil Microbiology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4870Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12992)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12992
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
72322Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49385.1StrainInfo: A central database for resolving microbial strain identifiers