Strain identifier

BacDive ID: 1938

Type strain: Yes

Species: Burkholderia pyrrocinia

Strain Designation: 2327

Strain history: CIP <- 1999, CFBP <- LMG <- ATCC, Pseudomonas pyrrocinia <- Fujisawa Pharm. Co.: strain 2327

NCBI tax ID(s): 60550 (species)

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General

@ref: 4037

BacDive-ID: 1938

DSM-Number: 10685

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, motile

description: Burkholderia pyrrocinia 2327 is an aerobe, mesophilic, motile bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 60550
  • Matching level: species

strain history

@refhistory
4037<- ATCC <- Fujisawa Pharm. Co., Ltd.; 2327
122093CIP <- 1999, CFBP <- LMG <- ATCC, Pseudomonas pyrrocinia <- Fujisawa Pharm. Co.: strain 2327

doi: 10.13145/bacdive1938.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Burkholderiaceae
  • genus: Burkholderia
  • species: Burkholderia pyrrocinia
  • full scientific name: Burkholderia pyrrocinia (Imanaka et al. 1965) Vandamme et al. 1997

  • synonyms

    @refsynonym
    20215Pseudomonas pyrrocinia
    20215Burkholderia pyrrocinia

@ref: 4037

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Burkholderiaceae

genus: Burkholderia

species: Burkholderia pyrrocinia

full scientific name: Burkholderia pyrrocinia (Imanaka et al. 1965) Vandamme et al. 1997 emend. Storms et al. 2004

strain designation: 2327

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes91.998
6948099.984negative
122093yesnegativerod-shaped

colony morphology

  • @ref: 122093

pigmentation

  • @ref: 122093
  • production: no
  • name: Pyocyanin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4037NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
4037CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
40270MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
122093CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
4037positivegrowth28mesophilic
40270positivegrowth30mesophilic
55248positivegrowth37mesophilic
122093positivegrowth25-37mesophilic
122093nogrowth5psychrophilic
122093nogrowth10psychrophilic
122093nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
55248aerobe
122093obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.963

compound production

  • @ref: 4037
  • compound: pyrrolnitrin

halophily

@refsaltgrowthtested relationconcentration
122093NaClpositivegrowth0-4 %
122093NaClnogrowth6 %
122093NaClnogrowth8 %
122093NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
68369malate+assimilation25115
68369adipate+assimilation17128
68369decanoate+assimilation27689
68369gluconate+assimilation24265
68369maltose-assimilation17306
68369N-acetylglucosamine+assimilation59640
68369D-mannitol+assimilation16899
68369D-mannose+assimilation16024
68369L-arabinose+assimilation30849
68369D-glucose+assimilation17634
68369gelatin-hydrolysis5291
68369esculin-hydrolysis4853
68369urea-hydrolysis16199
68369arginine-hydrolysis29016
68369tryptophan-energy source27897
68369nitrate-reduction17632
122093citrate+carbon source16947
122093esculin+hydrolysis4853
122093nitrate-reduction17632
122093nitrite-reduction16301
122093nitrate-respiration17632

antibiotic resistance

  • @ref: 122093
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12209335581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
4037catalase+1.11.1.6
4037cytochrome-c oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
122093oxidase-
122093beta-galactosidase+3.2.1.23
122093alcohol dehydrogenase-1.1.1.1
122093gelatinase+/-
122093amylase-
122093DNase-
122093caseinase+3.4.21.50
122093catalase+1.11.1.6
122093tween esterase+
122093lecithinase+
122093lipase+
122093lysine decarboxylase-4.1.1.18
122093ornithine decarboxylase-4.1.1.17
122093protease+
122093tryptophan deaminase-
122093urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    55248C12:02.912
    55248C14:00.714
    55248C16:01716
    55248C18:00.718
    55248C13:1 at 12-130.612.931
    55248C14:0 3OH/C16:1 ISO I4.315.485
    55248C16:0 2OH2.517.233
    55248C16:0 3OH3.217.52
    55248C16:1 2OH1.517.047
    55248C16:1 ω7c9.915.819
    55248C17:0 CYCLO1516.888
    55248C18:1 2OH3.519.088
    55248C18:1 ω7c /12t/9t30.817.824
    55248C18:1 ω9c0.717.769
    55248C18:2 ω6,9c/C18:0 ANTE0.817.724
    55248C19:0 CYCLO ω8c5.918.9
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122093-+++++----++----++--

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
4037--+----++++++-+++++++
4037-------++++++-+++++++/-
4037-------++++++-+++++++
4037--+----++++++-+++++++

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122093-------------------------------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122093+++++--+-----+-++-++++--+-++++++++--++-+---+++--++++-++++++++++++--+---+++++++++++--+++-++++++++++-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
4037soilJapanJPNAsia
55248Soil
122093Environment, Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
40371Risk group (German classification)
1220931Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Burkholderia pyrrocinia gene for 16S rRNA, strain: ATCC 15958AB0213691506ena60550
4037Burkholderia pyrrocinia strain CIP 105874 16S ribosomal RNA gene, partial sequenceEU0241821313ena60550

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Burkholderia pyrrocinia DSM 10685GCA_001028665completencbi60550
66792Burkholderia pyrrocinia LMG 14191GCA_902832895scaffoldncbi60550
66792Burkholderia pyrrocinia strain DSM 1068560550.16plasmidpatric60550
66792Burkholderia pyrrocinia strain DSM 1068560550.9completepatric60550
66792Burkholderia pyrrocinia strain LMG 1419160550.39wgspatric60550
66792Burkholderia pyrrocinia DSM 106852630968762completeimg60550

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes88.059no
flagellatedno76.751no
gram-positiveno98.221no
anaerobicno98.634no
aerobicyes88.843no
halophileno92.636no
spore-formingno92.299no
thermophileno99.666yes
glucose-utilyes92.745yes
glucose-fermentno83.749no

External links

@ref: 4037

culture collection no.: DSM 10685, ATCC 15958, CCUG 41877, NCIMB 13695, LMG 14191, CIP 105874, CCRC 14939, CFBP 4794

straininfo link

  • @ref: 71583
  • straininfo: 92256

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology3417677Purification and characterization of a novel bacterial non-heme chloroperoxidase from Pseudomonas pyrrocinia.Wiesner W, van Pee KH, Lingens FJ Biol ChemS0021-9258(18)68301-21988Amino Acid Sequence, Amino Acids/analysis, Bromides/metabolism, Chloride Peroxidase/antagonists & inhibitors/*isolation & purification/metabolism, Chlorides/metabolism, Chromatography, Drug Stability, Fluorides/pharmacology, Iodides/metabolism, Isoelectric Point, Kinetics, Macromolecular Substances, Metals/analysis, Molecular Sequence Data, Molecular Weight, Peroxidases/*isolation & purification, Pseudomonas/*enzymology, Pyrroles/metabolism, Spectrophotometry, Substrate Specificity, TemperatureMetabolism
Enzymology4091967Purification and properties of bromoperoxidase from Pseudomonas pyrrocinia.Wiesner W, van Pee KH, Lingens FBiol Chem Hoppe Seyler10.1515/bchm3.1985.366.2.10851985Electrophoresis, Polyacrylamide Gel, Hydrogen-Ion Concentration, Isoelectric Focusing, Molecular Weight, Peroxidases/*isolation & purification, Pseudomonas/*enzymology, UltracentrifugationPhylogeny
Metabolism6862384The metabolism of tryptophan and 7-chlorotryptophan in Pseudomonas pyrrocinia and Pseudomonas aureofaciens.Lubbe C, van Pee KH, Salcher O, Lingens FHoppe Seylers Z Physiol Chem10.1515/bchm2.1983.364.1.4471983Amino Acid Isomerases/metabolism, Indoles/isolation & purification, Kinetics, Pseudomonas/*metabolism, Species Specificity, Transaminases/metabolism, Tryptophan/*analogs & derivatives/*metabolism, Tryptophan TransaminaseEnzymology
19719693Polymorphisms within the prnD and pltC genes from pyrrolnitrin and pyoluteorin-producing Pseudomonas and Burkholderia spp.Souza JT, Raaijmakers JMFEMS Microbiol Ecol10.1111/j.1574-6941.2003.tb01042.x2003
Genetics26150017Complete genome sequence of Burkholderia pyrrocinia 2327(T), the first industrial bacterium which produced antifungal antibiotic pyrrolnitrin.Kwak Y, Shin JHJ Biotechnol10.1016/j.jbiotec.2015.06.4202015Anti-Bacterial Agents/*pharmacology, Antifungal Agents/*pharmacology, Base Sequence, Burkholderia/*genetics, *Genome, Bacterial, *Industrial Microbiology, Pyrrolnitrin/*pharmacologyBiotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4037Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10685)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10685
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40270Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17941
55248Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 41877)https://www.ccug.se/strain?id=41877
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71583Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92256.1StrainInfo: A central database for resolving microbial strain identifiers
122093Curators of the CIPCollection of Institut Pasteur (CIP 105874)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105874