Strain identifier
BacDive ID: 14787
Type strain:
Species: Streptococcus uberis
Strain history: CIP <- 1988, NCTC <- K. Diernhofer, type I
NCBI tax ID(s): 1349 (species)
General
@ref: 8919
BacDive-ID: 14787
DSM-Number: 20569
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, coccus-shaped, human pathogen
description: Streptococcus uberis DSM 20569 is a microaerophile, mesophilic, Gram-positive human pathogen of the family Streptococcaceae.
NCBI tax id
- NCBI tax id: 1349
- Matching level: species
strain history
@ref | history |
---|---|
8919 | <- NCTC; NCTC 3858 <- K. Diernhofer; |
67770 | DSM 20569 <-- NCTC 3858 <-- K. Diernhofer. |
123705 | CIP <- 1988, NCTC <- K. Diernhofer, type I |
doi: 10.13145/bacdive14787.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Streptococcaceae
- genus: Streptococcus
- species: Streptococcus uberis
- full scientific name: Streptococcus uberis Diernhofer 1932 (Approved Lists 1980)
@ref: 8919
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Streptococcaceae
genus: Streptococcus
species: Streptococcus uberis
full scientific name: Streptococcus uberis Diernhofer 1932
type strain: yes
Morphology
cell morphology
- @ref: 123705
- gram stain: positive
- cell shape: coccus-shaped
- motility: no
colony morphology
@ref | type of hemolysis | incubation period | hemolysis ability |
---|---|---|---|
8919 | gamma | 1-2 days | |
123705 | 1 |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
8919 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
8919 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
41694 | MEDIUM 6 - Columbia agar with 10 % horse blood | yes | Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml) | |
123705 | CIP Medium 6 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
8919 | positive | growth | 37 | mesophilic |
41694 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
123705 | positive | growth | 30 | mesophilic |
123705 | no | growth | 10 | psychrophilic |
123705 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
8919 | microaerophile |
123705 | facultative anaerobe |
spore formation
- @ref: 123705
- spore formation: no
halophily
- @ref: 123705
- salt: NaCl
- growth: no
- tested relation: growth
- concentration: 6.5 %
murein
- @ref: 8919
- murein short key: A11.05
- type: A3alpha L-Lys-L-Ala2
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68370 | 28087 | glycogen | - | builds acid from |
68370 | 28017 | starch | - | builds acid from |
68370 | 16634 | raffinose | - | builds acid from |
68370 | 15443 | inulin | + | builds acid from |
68370 | 27082 | trehalose | + | builds acid from |
68370 | 17716 | lactose | + | builds acid from |
68370 | 17924 | D-sorbitol | + | builds acid from |
68370 | 16899 | D-mannitol | + | builds acid from |
68370 | 30849 | L-arabinose | - | builds acid from |
68370 | 16988 | D-ribose | + | builds acid from |
68370 | 29016 | arginine | + | hydrolysis |
68370 | 4853 | esculin | + | hydrolysis |
68370 | 606565 | hippurate | + | hydrolysis |
123705 | 4853 | esculin | + | hydrolysis |
123705 | 17632 | nitrate | - | builds gas from |
123705 | 17632 | nitrate | - | reduction |
123705 | 16301 | nitrite | - | reduction |
68381 | 29016 | arginine | + | hydrolysis |
68381 | 16988 | D-ribose | + | builds acid from |
68381 | 16899 | D-mannitol | + | builds acid from |
68381 | 30911 | sorbitol | + | builds acid from |
68381 | 17716 | lactose | + | builds acid from |
68381 | 27082 | trehalose | + | builds acid from |
68381 | 16634 | raffinose | - | builds acid from |
68381 | 17992 | sucrose | + | builds acid from |
68381 | 30849 | L-arabinose | - | builds acid from |
68381 | 18333 | D-arabitol | - | builds acid from |
68381 | 40585 | alpha-cyclodextrin | - | builds acid from |
68381 | 606565 | hippurate | + | hydrolysis |
68381 | 28087 | glycogen | - | builds acid from |
68381 | 27941 | pullulan | - | builds acid from |
68381 | 17306 | maltose | + | builds acid from |
68381 | 28053 | melibiose | - | builds acid from |
68381 | 6731 | melezitose | - | builds acid from |
68381 | 320055 | methyl beta-D-glucopyranoside | + | builds acid from |
68381 | 16443 | D-tagatose | - | builds acid from |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68370 | 15688 | acetoin | yes |
123705 | 35581 | indole | no |
68381 | 15688 | acetoin | yes |
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test |
---|---|---|---|
68381 | 15688 | acetoin | + |
68370 | 15688 | acetoin | + |
123705 | 15688 | acetoin | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
8919 | cytochrome-c oxidase | - | 1.9.3.1 |
68381 | Alanyl-Phenylalanyl-Proline arylamidase | + | |
68381 | alpha-galactosidase | - | 3.2.1.22 |
68381 | beta-glucuronidase | + | 3.2.1.31 |
68381 | beta-glucosidase | + | 3.2.1.21 |
68381 | arginine dihydrolase | + | 3.5.3.6 |
68370 | arginine dihydrolase | + | 3.5.3.6 |
68370 | leucine arylamidase | + | 3.4.11.1 |
68370 | alkaline phosphatase | + | 3.1.3.1 |
68370 | beta-galactosidase | - | 3.2.1.23 |
68370 | alpha-galactosidase | - | 3.2.1.22 |
68370 | pyrrolidonyl arylamidase | + | 3.4.19.3 |
68370 | beta-glucosidase | + | 3.2.1.21 |
123705 | oxidase | - | |
123705 | beta-galactosidase | + | 3.2.1.23 |
123705 | alcohol dehydrogenase | + | 1.1.1.1 |
123705 | catalase | - | 1.11.1.6 |
123705 | lysine decarboxylase | - | 4.1.1.18 |
123705 | ornithine decarboxylase | - | 4.1.1.17 |
123705 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | + | 3.2.1.31 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
123705 | - | + | - | + | - | + | - | - | - | - | + | + | - | - | + | + | + | - | - | - |
API 20STR
@ref | VP | HIP | ESC | PYRA | alpha GAL | beta GUR | beta GAL | PAL | LAP | ADH | RIB | ARA | MAN | SOR | LAC | TRE | INU | RAF | AMD | GLYG | beta HEM |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
8919 | + | + | + | + | - | + | - | + | + | + | + | - | + | + | + | + | + | - | - | - | |
8919 | + | + | + | + | - | - | - | + | + | + | + | - | + | + | + | + | + | - | - | - | |
8919 | + | + | + | + | - | + | - | + | + | + | + | - | + | + | + | + | + | - | - | - | - |
API rID32STR
@ref | ADH Arg | beta GLU | beta GAR | beta GUR | alpha GAL | PAL | RIB | MAN | SOR | LAC | TRE | RAF | SAC | LARA | DARL | CDEX | VP | APPA | beta GAL | PyrA | beta NAG | GTA | HIP | GLYG | PUL | MAL | MEL | MLZ | Mbeta DG | TAG | beta MAN | URE |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
8919 | + | + | - | + | - | + | + | + | + | + | + | - | + | - | - | - | + | + | - | - | + | + | + | - | - | + | - | - | + | - | + | - |
8919 | + | + | + | + | - | + | + | + | + | + | + | - | + | - | - | - | + | + | - | + | + | - | + | - | - | + | - | - | + | - | + | - |
8919 | + | + | + | + | - | + | + | + | + | + | + | - | + | - | - | - | + | + | - | + | + | - | + | - | - | + | - | - | + | - | + | - |
8919 | + | + | + | + | - | + | + | + | + | + | + | - | + | - | - | - | + | + | - | + | + | + | + | - | - | + | - | - | + | - | + | - |
8919 | + | + | + | + | - | + | + | + | + | + | + | - | + | - | - | - | + | + | - | + | + | + | + | - | - | + | - | - | + | - | + | - |
8919 | + | + | + | + | - | + | + | + | + | + | + | - | + | - | - | - | + | + | +/- | + | + | + | + | - | - | + | - | - | + | - | + | +/- |
8919 | + | + | + | + | - | + | + | + | + | + | + | - | + | - | - | - | + | + | - | + | + | + | + | - | - | + | - | - | + | - | + | - |
8919 | + | + | + | + | - | - | + | + | + | + | + | - | + | - | - | - | + | + | - | - | - | - | + | - | - | + | - | - | + | - | - | - |
8919 | + | + | + | + | - | - | + | + | + | + | + | - | + | - | - | - | + | + | - | - | - | - | + | - | - | + | - | - | + | - | - | - |
8919 | + | + | + | + | - | + | + | + | + | + | + | - | + | - | - | - | + | + | - | + | + | + | + | - | - | + | - | - | + | - | + | - |
Isolation, sampling and environmental information
isolation
@ref | geographic location | country | origin.country | continent | sample type | isolation date |
---|---|---|---|---|---|---|
41694 | Vienne | Austria | AUT | Europe | ||
123705 | Vienna | Austria | AUT | Europe | Unknown source | 1933 |
taxonmaps
- @ref: 69479
- File name: preview.99_2664.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4;96_79;97_1693;98_2063;99_2664&stattab=map
- Last taxonomy: Streptococcus uberis subclade
- 16S sequence: LC071829
- Sequence Identity:
- Total samples: 26016
- soil counts: 829
- aquatic counts: 630
- animal counts: 24413
- plant counts: 144
Safety information
risk assessment
@ref | pathogenicity human | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|---|
8919 | yes | yes | 2 | Risk group (German classification) |
123705 | 2 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Streptococcus uberis 16S-23S ribosomal RNA intergenic spacer, complete sequence, 23S ribosomal RNA partial sequence | AF255657 | 585 | ena | 1349 |
20218 | Streptococcus uberis strain ATCC 19436 16S-23S ribosomal RNA intergenic spacer, complete sequence | AY347538 | 338 | ena | 1349 |
20218 | Streptococcus uberis strain ATCC 19436 16S ribosomal RNA gene, partial sequence | JN630842 | 803 | ena | 1349 |
8919 | Streptococcus uberis gene for 16S rRNA, strain:JCM 5709 | AB023573 | 1501 | ena | 1349 |
67770 | Streptococcus uberis DNA for 16S rRNA, strain ATCC 19436, NCTC 3858 | AB002526 | 1442 | ena | 1349 |
67770 | Streptococcus uberis gene for 16S ribosomal RNA, partial sequence, strain: JCM 5709 | LC071829 | 1481 | ena | 1349 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Streptococcus uberis NCTC3858 | GCA_900475595 | complete | ncbi | 1349 |
66792 | Streptococcus uberis strain NCTC3858 | 1349.243 | complete | patric | 1349 |
66792 | Streptococcus uberis NCTC 3858 | 2870424623 | complete | img | 1349 |
GC content
@ref | GC-content | method |
---|---|---|
8919 | 37.5 | |
67770 | 36.5 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
flagellated | no | 97.162 | no |
gram-positive | yes | 94.049 | no |
anaerobic | no | 95.403 | yes |
aerobic | no | 95.935 | yes |
halophile | yes | 85.862 | no |
spore-forming | no | 94.905 | no |
thermophile | no | 99.313 | yes |
glucose-util | yes | 88.67 | no |
motile | no | 95.655 | no |
glucose-ferment | yes | 83.997 | no |
External links
@ref: 8919
culture collection no.: DSM 20569, ATCC 19436, CCUG 17930, JCM 5709, NCDO 2038, NCTC 3858, BCRC 12579, CCUG 27579, CECT 994, CIP 103219, LMG 9465, NCIMB 702038
straininfo link
- @ref: 83904
- straininfo: 125888
literature
Pubmed-ID | title | authors | journal | DOI | year | mesh | topic | topic2 |
---|---|---|---|---|---|---|---|---|
8434140 | An R-like protein of Streptococcus uberis stimulates opsonising antibodies. | Groschup MH, Timoney JF | Res Vet Sci | 10.1016/0034-5288(93)90021-7 | 1993 | Animals, Antibodies, Bacterial/*blood/immunology, Antigens, Bacterial/*immunology, Bacterial Proteins/*immunology, Cattle, Guinea Pigs, Opsonin Proteins/*blood/immunology, Streptococcus/*immunology | ||
10024545 | Purification and cloning of a streptokinase from Streptococcus uberis. | Johnsen LB, Poulsen K, Kilian M, Petersen TE | Infect Immun | 10.1128/IAI.67.3.1072-1078.1999 | 1999 | Amino Acid Sequence, Animals, Bacterial Vaccines/immunology, Base Sequence, Cattle, Cloning, Molecular, Molecular Sequence Data, Plasminogen Activators/genetics/*isolation & purification, Streptococcus/*enzymology/immunology, Streptokinase/genetics/immunology/*isolation & purification | Enzymology | Genetics |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
8919 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20569) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-20569 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
41694 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/14993 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68370 | Automatically annotated from API 20STR | |||||
68381 | Automatically annotated from API rID32STR | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
83904 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID125888.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
123705 | Curators of the CIP | Collection of Institut Pasteur (CIP 103219) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103219 |