Strain identifier
BacDive ID: 14335
Type strain:
Species: Alkalispirochaeta americana
Strain Designation: ASpG1
Strain history: <- E. V. Pikuta, Center Space Plasma and Aeronomic Res. (CSPAR), Univ. Alabama Huntsville, USA; ASpG1
NCBI tax ID(s): 159291 (species)
General
@ref: 5589
BacDive-ID: 14335
DSM-Number: 14872
keywords: genome sequence, 16S sequence, Bacteria, obligate anaerobe, mesophilic, Gram-negative
description: Alkalispirochaeta americana ASpG1 is an obligate anaerobe, mesophilic, Gram-negative bacterium that was isolated from mud sediment of an alkaline, hypersaline, meromictic soda lake.
NCBI tax id
- NCBI tax id: 159291
- Matching level: species
strain history
- @ref: 5589
- history: <- E. V. Pikuta, Center Space Plasma and Aeronomic Res. (CSPAR), Univ. Alabama Huntsville, USA; ASpG1
doi: 10.13145/bacdive14335.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/spirochaetota
- domain: Bacteria
- phylum: Spirochaetota
- class: Spirochaetia
- order: Spirochaetales
- family: Spirochaetaceae
- genus: Alkalispirochaeta
- species: Alkalispirochaeta americana
- full scientific name: Alkalispirochaeta americana (Hoover et al. 2003) Sravanthi et al. 2016
synonyms
- @ref: 20215
- synonym: Spirochaeta americana
@ref: 5589
domain: Bacteria
phylum: Spirochaetes
class: Spirochaetia
order: Spirochaetales
family: Spirochaetaceae
genus: Alkalispirochaeta
species: Alkalispirochaeta americana
full scientific name: Alkalispirochaeta americana (Hoover et al. 2003) Sravanthi et al. 2016
strain designation: ASpG1
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: negative
- confidence: 99.945
Culture and growth conditions
culture medium
- @ref: 5589
- name: SPIROCHAETA AMERICANA MEDIUM (DSMZ Medium 1165)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1165
- composition: Name: SPIROCHAETA AMERICANA MEDIUM (DSMZ Medium 1165) Composition: NaCl 30.0 g/l NaHCO3 24.0 g/l D-Glucose 5.0 g/l Na2CO3 2.76 g/l NH4Cl 1.0 g/l Yeast extract 0.5 g/l Na2S x 9 H2O 0.4 g/l KCl 0.2 g/l K2HPO4 0.2 g/l MgCl2 x 6 H2O 0.1 g/l MnCl2 x 4 H2O 0.0036 g/l Fe(NH4)2(SO4)2 x 12 H2O 0.002 g/l ZnSO4 x 7 H2O 0.001 g/l CoCl2 x 6 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Resazurin 0.001 g/l NiCl2 0.0005 g/l H3BO3 0.0001 g/l CuSO4 x 5 H2O 0.0001 g/l AlK(SO4)2 x 12 H2O 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l Pyridoxine hydrochloride 2e-05 g/l Lipoic acid 1e-05 g/l p-Aminobenzoic acid 1e-05 g/l D-Calcium pantothenate 1e-05 g/l Nicotinic acid 1e-05 g/l Riboflavin 1e-05 g/l Thiamine-HCl x 2 H2O 1e-05 g/l Folic acid 4e-06 g/l Biotin 4e-06 g/l Vitamin B12 2e-07 g/l HCl Distilled water
culture temp
- @ref: 5589
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance | confidence |
---|---|---|
5589 | obligate anaerobe | |
69480 | anaerobe | 99.086 |
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.984 |
Isolation, sampling and environmental information
isolation
- @ref: 5589
- sample type: mud sediment of an alkaline, hypersaline, meromictic soda lake
- geographic location: northern California, Mono Lake
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Terrestrial | #Mud (Sludge) |
#Environmental | #Aquatic | #Sediment |
#Condition | #Alkaline | |
#Condition | #Saline |
taxonmaps
- @ref: 69479
- File name: preview.99_7454.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_642;96_3481;97_4268;98_5462;99_7454&stattab=map
- Last taxonomy: Alkalispirochaeta
- 16S sequence: AF373921
- Sequence Identity:
- Total samples: 382
- soil counts: 26
- aquatic counts: 304
- animal counts: 52
Safety information
risk assessment
- @ref: 5589
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 5589
- description: Spirochaeta americana ASpG1 16S ribosomal RNA gene, partial sequence
- accession: AF373921
- length: 1467
- database: ena
- NCBI tax ID: 159291
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Alkalispirochaeta americana ASpG1 | GCA_900156105 | scaffold | ncbi | 159291 |
66792 | Alkalispirochaeta americana ASpG1 | 2706795025 | draft | img | 159291 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
motile | yes | 86.79 | no |
flagellated | no | 71.564 | no |
gram-positive | no | 96.239 | no |
anaerobic | yes | 97.041 | yes |
aerobic | no | 97.816 | yes |
halophile | yes | 52.703 | no |
spore-forming | no | 91.083 | no |
thermophile | no | 71.915 | no |
glucose-util | yes | 89.597 | no |
glucose-ferment | yes | 66.623 | no |
External links
@ref: 5589
culture collection no.: DSM 14872, ATCC BAA 392
straininfo link
- @ref: 83480
- straininfo: 100107
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 12807206 | Spirochaeta americana sp. nov., a new haloalkaliphilic, obligately anaerobic spirochaete isolated from soda Mono Lake in California. | Hoover RB, Pikuta EV, Bej AK, Marsic D, Whitman WB, Tang J, Krader P | Int J Syst Evol Microbiol | 10.1099/ijs.0.02535-0 | 2003 | Anaerobiosis, Bacterial Typing Techniques, Base Composition, California, Culture Media, DNA, Ribosomal/analysis, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/*metabolism, Spirochaeta/*classification/genetics/*growth & development/isolation & purification/metabolism, *Water Microbiology | Cultivation |
Phylogeny | 23148099 | Spirochaeta sphaeroplastigenens sp. nov., a halo-alkaliphilic, obligately anaerobic spirochaete isolated from soda lake Lonar. | Vishnuvardhan Reddy S, Aspana S, Tushar DL, Sasikala C, Ramana CV | Int J Syst Evol Microbiol | 10.1099/ijs.0.046292-0 | 2012 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, India, Lakes/microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spirochaeta/*classification/genetics/isolation & purification, *Water Microbiology | Genetics |
Phylogeny | 26377069 | Spirochaeta odontotermitis sp. nov., an obligately anaerobic, cellulolytic, halotolerant, alkaliphilic spirochaete isolated from the termite Odontotermes obesus (Rambur) gut. | Sravanthi T, Tushar L, Sasikala C, Ramana CV | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000616 | 2015 | Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, India, Isoptera/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spirochaeta/*classification/genetics/isolation & purification | Genetics |
Phylogeny | 26704619 | Alkalispirochaeta cellulosivorans gen. nov., sp. nov., a cellulose-hydrolysing, alkaliphilic, halotolerant bacterium isolated from the gut of a wood-eating cockroach (Cryptocercus punctulatus), and reclassification of four species of Spirochaeta as new combinations within Alkalispirochaeta gen. nov. | Sravanthi T, Tushar L, Sasikala C, Ramana CV | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000865 | 2015 | Animals, Bacterial Typing Techniques, Base Composition, Cellulose/metabolism, Cockroaches/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, India, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spirochaeta/*classification/genetics/isolation & purification, Wood | Genetics |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
5589 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14872) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-14872 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
83480 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID100107.1 | StrainInfo: A central database for resolving microbial strain identifiers |