Strain identifier

BacDive ID: 130531

Type strain: Yes

Species: Mesorhizobium hawassense

Strain Designation: AC99b

Strain history: <- LMG <- A. Willems, BCCM; AC99b

NCBI tax ID(s): 1209954 (species)

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General

@ref: 22039

BacDive-ID: 130531

DSM-Number: 29666

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative, motile, rod-shaped

description: Mesorhizobium hawassense AC99b is a mesophilic, Gram-negative, motile bacterium that was isolated from root nodule Sesbania sesban.

NCBI tax id

  • NCBI tax id: 1209954
  • Matching level: species

strain history

  • @ref: 22039
  • history: <- LMG <- A. Willems, BCCM; AC99b

doi: 10.13145/bacdive130531.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Phyllobacteriaceae
  • genus: Mesorhizobium
  • species: Mesorhizobium hawassense
  • full scientific name: Mesorhizobium hawassense Degefu et al. 2013

@ref: 22039

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Phyllobacteriaceae

genus: Mesorhizobium

species: Mesorhizobium hawassense

full scientific name: Mesorhizobium hawassense Degefu et al. 2013

strain designation: AC99b

type strain: yes

Morphology

cell morphology

  • @ref: 30772
  • gram stain: negative
  • cell length: 2.7 µm
  • cell width: 0.3 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 22039
  • incubation period: 3-7 days

pigmentation

  • @ref: 30772
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
22039RHIZOBIUM MEDIUM (DSMZ Medium 98)yeshttps://mediadive.dsmz.de/medium/98Name: RHIZOBIUM MEDIUM (DSMZ Medium 98) Composition: air-dried garden soil 80.0 g/l Agar 15.0 g/l Mannitol 10.0 g/l Yeast extract 1.0 g/l Na2CO3 0.2 g/l Distilled water
22039R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
22039positivegrowth30mesophilic
30772positivegrowth0-35

culture pH

  • @ref: 30772
  • ability: positive
  • type: growth
  • pH: 4.5-10
  • PH range: alkaliphile

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3077215740formate+carbon source
3077215792malonate+carbon source
3077217822serine+carbon source
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836930849L-arabinose+assimilation
683695291gelatin-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
22039catalase+1.11.1.6
22039cytochrome-c oxidase+1.9.3.1
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
22039+---++-+++++++------+
22039+--------+--+/--------

Isolation, sampling and environmental information

isolation

  • @ref: 22039
  • sample type: root nodule Sesbania sesban
  • host species: Sesbania sesban
  • geographic location: Wondogenet
  • country: Ethiopia
  • origin.country: ETH
  • continent: Africa

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Shrub (Scrub)
#Host Body-Site#Plant#Root nodule

Safety information

risk assessment

  • @ref: 22039
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 22039
  • description: Mesorhizobium hawassense strain AC99b 16S ribosomal RNA gene, partial sequence
  • accession: GQ847899
  • length: 1449
  • database: ena
  • NCBI tax ID: 1209954

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mesorhizobium hawassense AC99bGCA_003289945contigncbi1209954
66792Mesorhizobium hawassense DSM 296662928380500draftimg1209954

GC content

  • @ref: 22039
  • GC-content: 62.5

External links

@ref: 22039

culture collection no.: DSM 29666, HAMBI 3301, LMG 26968

straininfo link

  • @ref: 89054
  • straininfo: 380477

literature

  • topic: Phylogeny
  • Pubmed-ID: 22941297
  • title: Mesorhizobium shonense sp. nov., Mesorhizobium hawassense sp. nov. and Mesorhizobium abyssinicae sp. nov., isolated from root nodules of different agroforestry legume trees.
  • authors: Degefu T, Wolde-Meskel E, Liu B, Cleenwerck I, Willems A, Frostegard A
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.044032-0
  • year: 2012
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Ethiopia, Fabaceae/*microbiology, Fatty Acids/analysis, Genes, Bacterial, Mesorhizobium/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Root Nodules, Plant/*microbiology, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
22039Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-29666Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29666)
30772Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172710328776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68369Automatically annotated from API 20NE
89054Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID380477.1