Strain identifier

BacDive ID: 17889

Type strain: Yes

Species: Sinosporangium album

Strain Designation: 6014

Strain history: <- YQ Zhang, Inst. Med. Biotechnol., CAMS, China

NCBI tax ID(s): 504805 (species)

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General

@ref: 15644

BacDive-ID: 17889

DSM-Number: 45181

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Sinosporangium album 6014 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 504805
  • Matching level: species

strain history

@refhistory
15644<- Y.-Q. Zhang, Chinese Academy Medical Sci. and Peking Union Medical College, Beijing, China; strain 6014
67770KCTC 19655 <-- Y.-Q. Zhang 6014.
67771<- YQ Zhang, Inst. Med. Biotechnol., CAMS, China

doi: 10.13145/bacdive17889.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Streptosporangiales
  • family: Streptosporangiaceae
  • genus: Sinosporangium
  • species: Sinosporangium album
  • full scientific name: Sinosporangium album Zhang et al. 2011

@ref: 15644

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptosporangiaceae

genus: Sinosporangium

species: Sinosporangium album

full scientific name: Sinosporangium album Zhang et al. 2011

strain designation: 6014

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidence
29837positiveno
67771positive
69480no90.346
69480positive100

colony morphology

@refcolony colorincubation periodmedium used
18293Brown beige (1011)10-14 daysISP 2
1829310-14 daysISP 3
1829310-14 daysISP 4
18293Light ivory (1015)10-14 daysISP 5
18293Ivory (1014)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
18293yesAerial MyceliumSand yellow (9003)ISP 2
18293yesAerial MyceliumCream (9001)ISP 3
18293yesAerial MyceliumCream (9001)ISP 4
18293yesAerial MyceliumOyster white (1013)ISP 5
18293noISP 7
18293yesAerial MyceliumCream (9001)Suter with tyrosine
18293yesAerial MyceliumCream (9001)Suter without tyrosine

pigmentation

  • @ref: 29837
  • production: no

multimedia

  • @ref: 15644
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45181.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15644GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
15644GPHF-MEDIUM (DSMZ Medium 553)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium553.pdf
18293ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18293ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18293ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18293ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18293ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
15644positivegrowth28mesophilic
18293positiveoptimum28mesophilic
29837positivegrowth10-37
29837positiveoptimum28-32mesophilic
67770positivegrowth28mesophilic
67771positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
29837positivegrowth6.5-8.5alkaliphile
29837positiveoptimum7.0-7.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
29837aerobe
67771aerobe

spore formation

@refspore formationconfidence
29837yes
69481yes100
69480yes100

halophily

@refsaltgrowthtested relationconcentration
18293NaClpositivegrowth0 %
29837NaClpositiveoptimum1 %

observation

@refobservation
67770quinones: MK-9(H2), MK-9(H4)
67771quinones: MK-9(H2), MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1829317234glucose+
1829322599arabinose+
1829317992sucrose+
1829318222xylose-
1829317268myo-inositol+
1829337684mannose-
1829328757fructose+
1829326546rhamnose-
1829316634raffinose-
1829362968cellulose+
29837581435-dehydro-D-gluconate+carbon source
2983715963ribitol+carbon source
2983722599arabinose+carbon source
2983728260galactose+carbon source
2983717234glucose+carbon source
29837506227N-acetylglucosamine+carbon source
2983733942ribose+carbon source
2983727082trehalose+carbon source
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea+hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
29837acid phosphatase+3.1.3.2
29837alkaline phosphatase+3.1.3.1
29837catalase+1.11.1.6
68379gelatinase+
68379urease+3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18293-+/-++--++-++--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18293+++-++-+/--++/----+-+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
15644soilQinghai provinceChinaCHNAsia
67770SoilQinghai ProvinceChinaCHNAsia
67771From soilYunnan ProvinceChinaCHNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_132564.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_239;96_21830;97_27251;98_34712;99_132564&stattab=map
  • Last taxonomy: Sinosporangium album
  • 16S sequence: EU438912
  • Sequence Identity:
  • Total samples: 431
  • soil counts: 245
  • aquatic counts: 24
  • animal counts: 152
  • plant counts: 10

Safety information

risk assessment

  • @ref: 15644
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15644
  • description: Streptosporangineae bacterium 6014 16S ribosomal RNA gene, partial sequence
  • accession: EU438912
  • length: 1497
  • database: ena
  • NCBI tax ID: 504805

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Sinosporangium album strain CPCC 201354504805.3wgspatric504805
66792Sinosporangium album CPCC 2013542675903142draftimg504805
67770Sinosporangium album CPCC 201354GCA_900100605scaffoldncbi504805

GC content

@refGC-contentmethod
1564469.4high performance liquid chromatography (HPLC)
2983769.4

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
gram-positiveyes86.481yes
anaerobicno98.473yes
halophileno94.2yes
spore-formingyes93.451yes
glucose-utilyes87.763yes
aerobicyes88.93yes
thermophileno95.638yes
motileno93.39yes
flagellatedno97.793yes
glucose-fermentno95.577yes

External links

@ref: 15644

culture collection no.: DSM 45181, BCC 55026, KCTC 19655, TBRC 2209, JCM 19962, CPCC 201354

straininfo link

  • @ref: 86878
  • straininfo: 402771

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny20400672Sinosporangium album gen. nov., sp. nov., a new member of the suborder Streptosporangineae.Zhang YQ, Liu HY, Yu LY, Lee JC, Park DJ, Kim CJ, Xu LH, Jiang CL, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.022186-02010Actinomycetales/chemistry/*classification/genetics/isolation & purification, Amino Acids/analysis, Base Composition, Carbohydrates/analysis, China, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Glycolipids/analysis, Molecular Sequence Data, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil Microbiology, Vitamin K 2/analysisGenetics
Phylogeny31046875Bailinhaonella thermotolerans gen. nov., sp. nov., a new member of the order Streptosporangiales.Feng YZ, Yang LL, Gao S, Ji Y, Yin M, Zhao YR, Chunyu WX, Li P, Zhi XY, Tang SKInt J Syst Evol Microbiol10.1099/ijsem.0.0033792019Actinomycetales/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
15644Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45181)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45181
18293Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM45181.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29837Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2621328776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
86878Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID402771.1StrainInfo: A central database for resolving microbial strain identifiers