Strain identifier

BacDive ID: 16461

Type strain: Yes

Species: Streptomyces atriruber

Strain history: <- D. P. Labeda, NRRL <- J. M. Donahue, LDDC

NCBI tax ID(s): 545121 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 10641

BacDive-ID: 16461

DSM-Number: 41860

keywords: 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive, ovoid-shaped, animal pathogen

description: Streptomyces atriruber DSM 41860 is a spore-forming, mesophilic, Gram-positive animal pathogen that builds an aerial mycelium and was isolated from equine placenta.

NCBI tax id

  • NCBI tax id: 545121
  • Matching level: species

strain history

  • @ref: 10641
  • history: <- D. P. Labeda, NRRL <- J. M. Donahue, LDDC

doi: 10.13145/bacdive16461.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces atriruber
  • full scientific name: Streptomyces atriruber Labeda et al. 2009

@ref: 10641

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces atriruber

full scientific name: Streptomyces atriruber Labeda et al. 2009

type strain: yes

Morphology

cell morphology

  • @ref: 29221
  • gram stain: positive
  • cell length: 0.85 µm
  • cell width: 0.65 µm
  • cell shape: ovoid-shaped

colony morphology

@refcolony colorincubation periodmedium used
18726Sand yellow10-14 daysISP 2
18726Nut brown10-14 daysISP 3
18726Oxid red10-14 daysISP 4
18726Olive brown10-14 daysISP 5
18726Fawn brown10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium namecomplex namecomplex color
18726noISP 2
18726yesISP 3Aerial MyceliumPearl mouse grey (7048)
18726yesISP 4Aerial Mycelium
18726noISP 5
18726noISP 6
18726noISP 7

pigmentation

  • @ref: 29221
  • production: yes

multimedia

  • @ref: 10641
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_41860.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10641GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
18726ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18726ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18726ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18726ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18726ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
10641positivegrowth28mesophilic
18726positiveoptimum28mesophilic
29221positivegrowth15-37

Physiology and metabolism

spore formation

  • @ref: 29221
  • spore formation: yes

observation

  • @ref: 29221
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1872617234glucose+
1872622599arabinose+
1872617992sucrose-
1872618222xylose+
1872617268myo-inositol+
1872637684mannose+
1872628757fructose+
1872626546rhamnose+
1872616634raffinose+
1872662968cellulose-
2922115963ribitol+carbon source
2922122599arabinose+carbon source
2922129016arginine+carbon source
2922122653asparagine+carbon source
2922117057cellobiose+carbon source
2922123652dextrin+carbon source
2922128757fructose+carbon source
2922128260galactose+carbon source
2922117234glucose+carbon source
2922117754glycerol+carbon source
2922129864mannitol+carbon source
2922137684mannose+carbon source
2922128053melibiose+carbon source
2922117268myo-inositol+carbon source
2922116634raffinose+carbon source
2922126546rhamnose+carbon source
2922117814salicin+carbon source
2922117822serine+carbon source
2922117992sucrose+carbon source
2922126986threonine+carbon source
2922127082trehalose+carbon source
2922118222xylose+carbon source
292214853esculin+hydrolysis
2922117632nitrate+reduction

enzymes

@refvalueactivityec
29221gelatinase+
29221urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18726+++-++--+++---++++-

Isolation, sampling and environmental information

isolation

  • @ref: 10641
  • sample type: equine placenta
  • geographic location: Kentucky, Lexington
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Equidae (Horse)
#Host Body Product#Urogenital tract

Safety information

risk assessment

  • @ref: 10641
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 10641
  • description: Streptomyces atriruber strain NRRL B-24165 16S ribosomal RNA gene, partial sequence
  • accession: EU812169
  • length: 1481
  • database: ena
  • NCBI tax ID: 545121

External links

@ref: 10641

culture collection no.: DSM 41860, LDDC 6330-99, NRRL B-24165

straininfo link

  • @ref: 85497
  • straininfo: 402035

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19628594Streptomyces atriruber sp. nov. and Streptomyces silaceus sp. nov., two novel species of equine origin.Labeda DP, Price NP, Kroppenstedt RM, Donahue JM, Williams NM, Sells SFInt J Syst Evol Microbiol10.1099/ijs.0.008862-02009Animals, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Female, Horses/*microbiology, Molecular Sequence Data, Phylogeny, Placenta/*microbiology, Pregnancy, RNA, Ribosomal, 16S/genetics, Streptomyces/classification/genetics/*isolation & purificationGenetics
Phylogeny34410901Streptomyces xanthii sp. nov. and Streptomyces roseirectus sp. nov. isolated from a Chinese medicinal plant.Hu S, Wang Y, Wang J, Liu K, Tang X, Gao JInt J Syst Evol Microbiol10.1099/ijsem.0.0049622021Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, *Phylogeny, Plants, Medicinal/microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Streptomyces/classification/isolation & purification, Xanthium/*microbiologyTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
10641Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41860)https://www.dsmz.de/collection/catalogue/details/culture/DSM-41860
18726Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM41860.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29221Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2564228776041
68382Automatically annotated from API zym
85497Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID402035.1StrainInfo: A central database for resolving microbial strain identifiers