Strain identifier
BacDive ID: 16461
Type strain:
Species: Streptomyces atriruber
Strain history: <- D. P. Labeda, NRRL <- J. M. Donahue, LDDC
NCBI tax ID(s): 545121 (species)
General
@ref: 10641
BacDive-ID: 16461
DSM-Number: 41860
keywords: 16S sequence, Bacteria, spore-forming, mesophilic, Gram-positive, ovoid-shaped, animal pathogen
description: Streptomyces atriruber DSM 41860 is a spore-forming, mesophilic, Gram-positive animal pathogen that builds an aerial mycelium and was isolated from equine placenta.
NCBI tax id
- NCBI tax id: 545121
- Matching level: species
strain history
- @ref: 10641
- history: <- D. P. Labeda, NRRL <- J. M. Donahue, LDDC
doi: 10.13145/bacdive16461.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces atriruber
- full scientific name: Streptomyces atriruber Labeda et al. 2009
@ref: 10641
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces atriruber
full scientific name: Streptomyces atriruber Labeda et al. 2009
type strain: yes
Morphology
cell morphology
- @ref: 29221
- gram stain: positive
- cell length: 0.85 µm
- cell width: 0.65 µm
- cell shape: ovoid-shaped
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
18726 | Sand yellow | 10-14 days | ISP 2 |
18726 | Nut brown | 10-14 days | ISP 3 |
18726 | Oxid red | 10-14 days | ISP 4 |
18726 | Olive brown | 10-14 days | ISP 5 |
18726 | Fawn brown | 10-14 days | ISP 7 |
multicellular morphology
@ref | forms multicellular complex | medium name | complex name | complex color |
---|---|---|---|---|
18726 | no | ISP 2 | ||
18726 | yes | ISP 3 | Aerial Mycelium | Pearl mouse grey (7048) |
18726 | yes | ISP 4 | Aerial Mycelium | |
18726 | no | ISP 5 | ||
18726 | no | ISP 6 | ||
18726 | no | ISP 7 |
pigmentation
- @ref: 29221
- production: yes
multimedia
- @ref: 10641
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_41860.jpg
- caption: Medium 65 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
10641 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
18726 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
18726 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
18726 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
18726 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
18726 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
10641 | positive | growth | 28 | mesophilic |
18726 | positive | optimum | 28 | mesophilic |
29221 | positive | growth | 15-37 |
Physiology and metabolism
spore formation
- @ref: 29221
- spore formation: yes
observation
- @ref: 29221
- observation: aggregates in chains
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
18726 | 17234 | glucose | + | |
18726 | 22599 | arabinose | + | |
18726 | 17992 | sucrose | - | |
18726 | 18222 | xylose | + | |
18726 | 17268 | myo-inositol | + | |
18726 | 37684 | mannose | + | |
18726 | 28757 | fructose | + | |
18726 | 26546 | rhamnose | + | |
18726 | 16634 | raffinose | + | |
18726 | 62968 | cellulose | - | |
29221 | 15963 | ribitol | + | carbon source |
29221 | 22599 | arabinose | + | carbon source |
29221 | 29016 | arginine | + | carbon source |
29221 | 22653 | asparagine | + | carbon source |
29221 | 17057 | cellobiose | + | carbon source |
29221 | 23652 | dextrin | + | carbon source |
29221 | 28757 | fructose | + | carbon source |
29221 | 28260 | galactose | + | carbon source |
29221 | 17234 | glucose | + | carbon source |
29221 | 17754 | glycerol | + | carbon source |
29221 | 29864 | mannitol | + | carbon source |
29221 | 37684 | mannose | + | carbon source |
29221 | 28053 | melibiose | + | carbon source |
29221 | 17268 | myo-inositol | + | carbon source |
29221 | 16634 | raffinose | + | carbon source |
29221 | 26546 | rhamnose | + | carbon source |
29221 | 17814 | salicin | + | carbon source |
29221 | 17822 | serine | + | carbon source |
29221 | 17992 | sucrose | + | carbon source |
29221 | 26986 | threonine | + | carbon source |
29221 | 27082 | trehalose | + | carbon source |
29221 | 18222 | xylose | + | carbon source |
29221 | 4853 | esculin | + | hydrolysis |
29221 | 17632 | nitrate | + | reduction |
enzymes
@ref | value | activity | ec |
---|---|---|---|
29221 | gelatinase | + | |
29221 | urease | + | 3.5.1.5 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | + | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | + | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
68382 | alpha-mannosidase | + | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
18726 | + | + | + | - | + | + | - | - | + | + | + | - | - | - | + | + | + | + | - |
Isolation, sampling and environmental information
isolation
- @ref: 10641
- sample type: equine placenta
- geographic location: Kentucky, Lexington
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Host | #Mammals | #Equidae (Horse) |
#Host Body Product | #Urogenital tract |
Safety information
risk assessment
- @ref: 10641
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 10641
- description: Streptomyces atriruber strain NRRL B-24165 16S ribosomal RNA gene, partial sequence
- accession: EU812169
- length: 1481
- database: ena
- NCBI tax ID: 545121
External links
@ref: 10641
culture collection no.: DSM 41860, LDDC 6330-99, NRRL B-24165
straininfo link
- @ref: 85497
- straininfo: 402035
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 19628594 | Streptomyces atriruber sp. nov. and Streptomyces silaceus sp. nov., two novel species of equine origin. | Labeda DP, Price NP, Kroppenstedt RM, Donahue JM, Williams NM, Sells SF | Int J Syst Evol Microbiol | 10.1099/ijs.0.008862-0 | 2009 | Animals, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Female, Horses/*microbiology, Molecular Sequence Data, Phylogeny, Placenta/*microbiology, Pregnancy, RNA, Ribosomal, 16S/genetics, Streptomyces/classification/genetics/*isolation & purification | Genetics |
Phylogeny | 34410901 | Streptomyces xanthii sp. nov. and Streptomyces roseirectus sp. nov. isolated from a Chinese medicinal plant. | Hu S, Wang Y, Wang J, Liu K, Tang X, Gao J | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004962 | 2021 | Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, *Phylogeny, Plants, Medicinal/microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Streptomyces/classification/isolation & purification, Xanthium/*microbiology | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
10641 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41860) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-41860 | |||
18726 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM41860.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
29221 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 25642 | 28776041 | |
68382 | Automatically annotated from API zym | |||||
85497 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID402035.1 | StrainInfo: A central database for resolving microbial strain identifiers |