Strain identifier
BacDive ID: 1444
Type strain:
Species: Geobacillus stearothermophilus
Strain Designation: 10
Strain history: CIP <- 2001, DSMZ <- J. Stenesh
NCBI tax ID(s): 1891658 (species)
General
@ref: 4979
BacDive-ID: 1444
DSM-Number: 13240
keywords: genome sequence, Bacteria, obligate aerobe, spore-forming, thermophilic, motile
description: Geobacillus stearothermophilus 10 is an obligate aerobe, spore-forming, thermophilic bacterium that was isolated from hot springs.
NCBI tax id
- NCBI tax id: 1891658
- Matching level: species
strain history
@ref | history |
---|---|
4979 | <- J. Stenesh |
123298 | CIP <- 2001, DSMZ <- J. Stenesh |
doi: 10.13145/bacdive1444.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Geobacillus
- species: Geobacillus stearothermophilus
- full scientific name: Geobacillus stearothermophilus (Donk 1920) Nazina et al. 2001
synonyms
- @ref: 20215
- synonym: Bacillus stearothermophilus
@ref: 4979
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Geobacillus
species: Geobacillus stearothermophilus
full scientific name: Geobacillus sp.
strain designation: 10
type strain: no
Morphology
cell morphology
@ref | motility | confidence | gram stain | cell shape |
---|---|---|---|---|
69480 | yes | 94.271 | ||
69480 | 100 | positive | ||
123298 | no | positive | rod-shaped |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
4979 | CASO AGAR (MERCK 105458) (DSMZ Medium 220) | yes | https://mediadive.dsmz.de/medium/220 | Name: CASO AGAR (Merck 105458) (DSMZ Medium 220; with strain-specific modifications) Composition: Yeast extract 50.0 g/l Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water |
4979 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
34094 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
123298 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature | range | confidence |
---|---|---|---|---|---|
4979 | positive | growth | 55 | thermophilic | |
34094 | positive | growth | 55 | thermophilic | |
69480 | thermophilic | 100 | |||
123298 | positive | growth | 45-55 | thermophilic | |
123298 | no | growth | 10 | psychrophilic | |
123298 | no | growth | 22 | psychrophilic | |
123298 | no | growth | 30 | mesophilic | |
123298 | no | growth | 37 | mesophilic |
culture pH
- @ref: 123298
- ability: no
- type: growth
- pH: 6
Physiology and metabolism
oxygen tolerance
- @ref: 123298
- oxygen tolerance: obligate aerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | yes | 100 |
69480 | yes | 99.999 |
123298 | yes |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
123298 | NaCl | positive | growth | 0-2 % |
123298 | NaCl | no | growth | 4 % |
123298 | NaCl | no | growth | 6 % |
123298 | NaCl | no | growth | 8 % |
123298 | NaCl | no | growth | 10 % |
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
68371 | Potassium 5-ketogluconate | - | builds acid from | |
68371 | Potassium 2-ketogluconate | - | builds acid from | |
68371 | gluconate | - | builds acid from | 24265 |
68371 | L-arabitol | - | builds acid from | 18403 |
68371 | D-arabitol | - | builds acid from | 18333 |
68371 | L-fucose | - | builds acid from | 18287 |
68371 | D-fucose | - | builds acid from | 28847 |
68371 | D-tagatose | - | builds acid from | 16443 |
68371 | D-lyxose | - | builds acid from | 62318 |
68371 | gentiobiose | - | builds acid from | 28066 |
68371 | xylitol | - | builds acid from | 17151 |
68371 | inulin | - | builds acid from | 15443 |
68371 | cellobiose | - | builds acid from | 17057 |
68371 | amygdalin | - | builds acid from | 27613 |
68371 | N-acetylglucosamine | - | builds acid from | 59640 |
68371 | methyl alpha-D-glucopyranoside | - | builds acid from | 320061 |
68371 | methyl alpha-D-mannoside | - | builds acid from | 43943 |
68371 | D-sorbitol | - | builds acid from | 17924 |
68371 | D-mannitol | - | builds acid from | 16899 |
68371 | myo-inositol | - | builds acid from | 17268 |
68371 | galactitol | - | builds acid from | 16813 |
68371 | L-rhamnose | - | builds acid from | 62345 |
68371 | L-sorbose | - | builds acid from | 17266 |
68371 | methyl beta-D-xylopyranoside | - | builds acid from | 74863 |
68371 | ribitol | - | builds acid from | 15963 |
68371 | L-xylose | - | builds acid from | 65328 |
68371 | L-arabinose | - | builds acid from | 30849 |
68371 | D-arabinose | - | builds acid from | 17108 |
68371 | erythritol | - | builds acid from | 17113 |
68371 | glycerol | - | builds acid from | 17754 |
123298 | citrate | - | carbon source | 16947 |
123298 | esculin | + | hydrolysis | 4853 |
123298 | hippurate | + | hydrolysis | 606565 |
123298 | nitrate | - | reduction | 17632 |
123298 | nitrite | - | reduction | 16301 |
123298 | nitrate | - | respiration | 17632 |
metabolite production
- @ref: 123298
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | methylred-test |
---|---|---|---|---|
123298 | 15688 | acetoin | - | |
123298 | 17234 | glucose | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | + | 3.2.1.23 |
68382 | alpha-galactosidase | + | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | alpha-chymotrypsin | + | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | + | |
68382 | esterase (C 4) | + | |
68382 | alkaline phosphatase | - | 3.1.3.1 |
123298 | oxidase | + | |
123298 | beta-galactosidase | - | 3.2.1.23 |
123298 | alcohol dehydrogenase | + | 1.1.1.1 |
123298 | gelatinase | +/- | |
123298 | amylase | + | |
123298 | catalase | + | 1.11.1.6 |
123298 | tween esterase | - | |
123298 | gamma-glutamyltransferase | + | 2.3.2.2 |
123298 | lysine decarboxylase | - | 4.1.1.18 |
123298 | ornithine decarboxylase | - | 4.1.1.17 |
123298 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
123298 | - | - | + | + | - | + | - | - | - | + | + | + | + | + | - | - | - | - | - | - |
API 50CHac
@ref | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
123298 | - | - | - | - | +/- | +/- | - | - | - | +/- | +/- | +/- | +/- | - | - | - | - | - | - | - | - | - | - | +/- | +/- | +/- | - | +/- | +/- | +/- | +/- | +/- | - | +/- | +/- | +/- | +/- | - | - | +/- | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
4979 | hot springs | Montana, Yellow Stone National Park | USA | USA | North America |
123298 | Environment, Hot springs | Yellowstone, Montana | United States of America | USA | North America |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Thermal spring |
#Condition | #Thermophilic (>45°C) |
Safety information
risk assessment
- @ref: 123298
- biosafety level: 1
- biosafety level comment: Risk group (French classification)
Sequence information
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Geobacillus stearothermophilus 10 | 272567.8 | complete | patric | 272567 |
66792 | Geobacillus stearothermophilus 10 | 272567.18 | plasmid | patric | 272567 |
GC content
- @ref: 4979
- GC-content: 53
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 100 | no |
motile | yes | 90.421 | no |
gram-positive | yes | 83.886 | no |
anaerobic | no | 97.935 | no |
halophile | no | 96.2 | no |
spore-forming | yes | 96.213 | no |
glucose-util | yes | 91.053 | no |
aerobic | yes | 87.187 | no |
flagellated | yes | 87.035 | no |
thermophile | yes | 99.999 | yes |
glucose-ferment | no | 89.38 | no |
External links
@ref: 4979
culture collection no.: DSM 13240, CIP 106956
straininfo link
- @ref: 71094
- straininfo: 307563
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
4979 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13240) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-13240 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
34094 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/19143 | ||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
68371 | Automatically annotated from API 50CH acid | |||
68382 | Automatically annotated from API zym | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |
71094 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID307563.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
123298 | Curators of the CIP | Collection of Institut Pasteur (CIP 106956) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106956 |