Name: Malikia granosa P1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2978

Type strain:

Species: Malikia granosa

Strain Designation: P1

Culture col. no.: DSM 15619, CIP 108194, JCM 12706, CCUG 64012

Strain history: CIP <- 2003, DSMZ <- M. Wagner: strain P1

NCBI tax ID(s): 263067 (species)

SI-ID 138011

CIP 108194, DSM 15619, JCM 12706, CCUG 64012

Section

Malikia granosa P1 is an obligate aerobe, mesophilic, motile bacterium that was isolated from activated sludge.

motile
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Comamonadaceae*
	Genus	*Malikia*
	Species	**Malikia granosa**
	Full Scientific Name (LPSN)	Malikia granosa Spring et al. 2005

Information on morphological and physiological properties Morphology

[Ref.: #119727]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %100

[Ref.: #119727]	Cell shape	rod-shaped

[Ref.: #119727]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %92.819

[Ref.: #119727]	Pigment production	no
[Ref.: #119727]	Pigment name	Pyocyanin

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5912]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #5912]

Culture medium growth

[Ref.: #5912]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5912]

Culture medium composition

expand / minimize

[Ref.: #5912]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #5912]

Culture medium growth

[Ref.: #5912]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5912]

Culture medium composition

expand / minimize

[Ref.: #33210]

Culture medium

MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromati

[Ref.: #33210]

Culture medium growth

[Ref.: #33210]

Culture medium composition

expand / minimize

[Ref.: #119727]

Culture medium

CIP Medium 566

[Ref.: #119727]

Culture medium growth

[Ref.: #119727]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5912]		growth	28 °C
[Ref.: #33210]		growth	30 °C
[Ref.: #67770]		growth	35 °C
[Ref.: #119727]		growth	10-41 °C
[Ref.: #119727]	no	growth	5 °C

[Ref.: #5912]	Temperature range	mesophilic
[Ref.: #33210]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #119727]	Temperature range	psychrophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #119727]

Oxygen tolerance

obligate aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.997

	Salt	Tested relation		Salt conc.
[Ref.: #119727]	NaCl		growth	0	%
[Ref.: #119727]	NaCl	no	growth	2	%
[Ref.: #119727]	NaCl	no	growth	4	%
[Ref.: #119727]	NaCl	no	growth	6	%
[Ref.: #119727]	NaCl	no	growth	8	%
[Ref.: #119727]	NaCl	no	growth	10	%

[Ref.: #67770]

Observation

quinones: Q-8

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #119727]	citrate ChEBI	-	carbon source
[Ref.: #119727]	esculin ChEBI	-	hydrolysis
[Ref.: #119727]	nitrate ChEBI	+	reduction
[Ref.: #119727]	nitrate ChEBI	-	respiration
[Ref.: #119727]	nitrite ChEBI	-	reduction

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #119727]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #119727]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #119727]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #119727]	amylase	-
[Ref.: #119727]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #119727]	caseinase	-	3.4.21.50
[Ref.: #119727]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #119727]	DNase	+
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #119727]	gelatinase	-
[Ref.: #119727]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #119727]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #119727]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119727]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #119727]	oxidase	+
[Ref.: #119727]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #119727]	tryptophan deaminase	-
[Ref.: #119727]	tween esterase	-
[Ref.: #119727]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119727]	10971	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5912]	Sample type/isolated from	activated sludge
[Ref.: #5912]	Geographic location (country and/or sea, region)	Munich, Kläranlage Großlappen
[Ref.: #5912]	Country	Germany
[Ref.: #5912]	Country ISO 3 Code	DEU
[Ref.: #5912]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Activated sludge in a wastewater treatment plant
[Ref.: #67770]	Geographic location (country and/or sea, region)	Munich
[Ref.: #67770]	Country	Germany
[Ref.: #67770]	Country ISO 3 Code	DEU
[Ref.: #67770]	Continent	Europe

[Ref.: #119727]	Sample type/isolated from	Environment, Activated sludge
[Ref.: #119727]	Geographic location (country and/or sea, region)	München
[Ref.: #119727]	Country	Germany
[Ref.: #119727]	Country ISO 3 Code	DEU
[Ref.: #119727]	Continent	Europe
[Ref.: #119727]	Isolation date	1994
* marker position based on {}

Isolation sources categories

#Engineered

#Waste

#Activated sludge

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ627188 (>99% sequence identity) for Malikia granosa from Microbeatlas

Aquatic Samples	269
Soil Samples	13
Animal Samples	15
Plant Samples	15
Total Samples	312

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5912]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119727]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Malikia granosa 16S rRNA gene, type strain P1	AJ627188	1525	ENA	263067 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Malikia granosa P1	GCA_002980595	contig	GenBank	263067 tax ID
[Ref.: #66792]	Malikia granosa strain P1	263067.3	wgs	PATRIC	263067 tax ID	*
[Ref.: #66792]	Malikia granosa P1	2887120766	draft	JGI IMG/M	263067 tax ID	*

[Ref.: #5912]	GC-content	67 mol%
[Ref.: #67770]	GC-content	67 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Malikia granosa strain P1 PATRIC: 263067.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Malikia granosa strain P1 PATRIC: 263067.3
[Ref.: #69480]	motile	yes	88.881	no
[Ref.: #69480]	gram-positive	no	98.702	no
[Ref.: #69480]	anaerobic	no	95.391	no
[Ref.: #69480]	aerobic	yes	88.381	no
[Ref.: #69480]	halophile	no	93.19	no
[Ref.: #69480]	spore-forming	no	94.7	no
[Ref.: #69480]	glucose-util	no	50	no
[Ref.: #69480]	flagellated	yes	63.109	no
[Ref.: #69480]	thermophile	no	94.236	yes
[Ref.: #69480]	glucose-ferment	no	87.451	no

Availability in culture collections External links

[Ref.: #5912]

Culture collection no.

DSM 15619, CIP 108194, JCM 12706, CCUG 64012

[Ref.: #72500]

SI-ID 138011

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Malikia granosa gen. nov., sp. nov., a novel polyhydroxyalkanoate- and polyphosphate-accumulating bacterium isolated from activated sludge, and reclassification of Pseudomonas spinosa as Malikia spinosa comb. nov.	Spring S, Wagner M, Schumann P, Kampfer P	Int J Syst Evol Microbiol	10.1099/ijs.0.63356-0	2005	*

References

#5912

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15619

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#33210 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#72500

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID138011.1 )

#119727 Collection of Institut Pasteur ; Curators of the CIP; CIP 108194
* These data were automatically processed and therefore are not curated

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Malikia granosa strain P1

Malikia granosa strain P1

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