Name: Giesbergeria kuznetsovii D-412
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2960

Type strain:

Species: Giesbergeria kuznetsovii

Strain Designation: D-412

Culture col. no.: DSM 12827, CIP 109213

Strain history: CIP <- 2006, DSMZ <- G.A. Dubinina: strain D-412, Aquaspirillum sp.

NCBI tax ID(s): 314202 (species)

SI-ID 49375

DSM 12827, VKM B-2352, CIP 109213

Section

Giesbergeria kuznetsovii D-412 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from sulphide spring.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Comamonadaceae*
	Genus	*Giesbergeria*
	Species	**Giesbergeria kuznetsovii**
	Full Scientific Name (LPSN)	Giesbergeria kuznetsovii Grabovich et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #31673]	Gram stain	negative
[Ref.: #120518]	Gram stain	negative

[Ref.: #31673]	Cell width	1.2-1.5 µm

[Ref.: #31673]	Cell shape	rod-shaped
[Ref.: #120518]	Cell shape	rod-shaped

[Ref.: #31673]	Motility	yes
[Ref.: #120518]	Motility	yes

[Ref.: #31673]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4842]

Culture medium

AQUASPIRILLUM MEDIUM II (DSMZ Medium 1495)

[Ref.: #4842]

Culture medium growth

[Ref.: #4842]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4842]

Culture medium composition

expand / minimize

[Ref.: #4842]

Culture medium

SPIRILLUM MEDIUM (DSMZ Medium 37)

[Ref.: #4842]

Culture medium growth

[Ref.: #4842]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4842]

Culture medium composition

expand / minimize

[Ref.: #120518]

Culture medium

CIP Medium 65

[Ref.: #120518]

Culture medium growth

[Ref.: #120518]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #4842]	growth	28 °C
[Ref.: #31673]	growth	07-36 °C
[Ref.: #31673]	optimum	28 °C

[Ref.: #4842]	Temperature range	mesophilic
[Ref.: #31673]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31673]			optimum	7
[Ref.: #31673]			growth	6-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31673]	Oxygen tolerance	aerobe
[Ref.: #120518]	Oxygen tolerance	facultative anaerobe

[Ref.: #31673]

Ability of spore formation

[Ref.: #31673]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31673]	acetate ChEBI	+	carbon source
[Ref.: #31673]	alanine ChEBI	+	carbon source
[Ref.: #31673]	asparagine ChEBI	+	carbon source
[Ref.: #31673]	aspartate ChEBI	+	carbon source
[Ref.: #31673]	D-tryptophan ChEBI	+	carbon source
[Ref.: #31673]	formate ChEBI	+	carbon source
[Ref.: #31673]	glycerol ChEBI	+	carbon source
[Ref.: #31673]	glycine ChEBI	+	carbon source
[Ref.: #31673]	histidine ChEBI	+	carbon source
[Ref.: #31673]	lactate ChEBI	+	carbon source
[Ref.: #31673]	leucine ChEBI	+	carbon source
[Ref.: #31673]	malate ChEBI	+	carbon source
[Ref.: #120518]	nitrate ChEBI	+	reduction
[Ref.: #120518]	nitrite ChEBI	-	reduction
[Ref.: #31673]	ornithine ChEBI	+	carbon source
[Ref.: #31673]	phenylalanine ChEBI	+	carbon source
[Ref.: #31673]	proline ChEBI	+	carbon source
[Ref.: #31673]	propionate ChEBI	+	carbon source
[Ref.: #31673]	pyruvate ChEBI	+	carbon source
[Ref.: #31673]	serine ChEBI	+	carbon source
[Ref.: #31673]	succinate ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #120518]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #120518]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	+	3.2.1.31	* from API zym
[Ref.: #31673]	catalase	+	1.11.1.6
[Ref.: #120518]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #31673]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #120518]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120518]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120518]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #120518]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120518]	11723	-	+	+	+	-	-	-	-	-	-	+	+	+	+	+	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4842]	Sample type/isolated from	sulphide spring
[Ref.: #4842]	Geographic location (country and/or sea, region)	The Grimea
[Ref.: #4842]	Country	Russia
[Ref.: #4842]	Country ISO 3 Code	RUS
[Ref.: #4842]	Continent	Europe

[Ref.: #120518]	Sample type/isolated from	Environment, Sulphide spring
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Spring
#Condition	#Sulfuric	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY780907 (>99% sequence identity) for Giesbergeria from Microbeatlas

Aquatic Samples	2847
Soil Samples	266
Animal Samples	810
Plant Samples	117
Total Samples	4040

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4842]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120518]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #31673]	Giesbergeria kuznetsovii strain D-412 16S ribosomal RNA gene, partial sequence	AY780907	1491	GenBank	314202 tax ID

[Ref.: #31673]

GC-content

56.5 mol%

Availability in culture collections External links

[Ref.: #4842]

Culture collection no.

DSM 12827, CIP 109213

[Ref.: #72482]

SI-ID 49375

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Proposal of Giesbergeria voronezhensis gen. nov., sp. nov. and G. kuznetsovii sp. nov. and reclassification of [Aquaspirillum] anulus, [A.] sinuosum and [A.] giesbergeri as Giesbergeria anulus comb. nov., G. sinuosa comb. nov. and G. giesbergeri comb. nov., and [Aquaspirillum] metamorphum and [A.] psychrophilum as Simplicispira metamorpha gen. nov., comb. nov. and S. psychrophila comb. nov.	Grabovich M, Gavrish E, Kuever J, Lysenko AM, Podkopaeva D, Dubinina G	Int J Syst Evol Microbiol	10.1099/ijs.0.64027-0	2006	*

References

#4842

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 12827

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31673

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27952 (see below)

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#72482

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID49375.1 )

#120518 Collection of Institut Pasteur ; Curators of the CIP; CIP 109213

#27952

IJSEM 569 2006 ( DOI 10.1099/ijs.0.64027-0 , PubMed 16514029 )

* These data were automatically processed and therefore are not curated

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