Name: Leuconostoc gelidum SML9
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6830

Type strain:

Species: Leuconostoc gelidum

Strain Designation: SML9

Culture col. no.: DSM 5578, ATCC 49366, NCFB 2775, NCIMB 12897, JCM 9697, CCM 4189, CCUG 30198, CCUG 32249, CCUG 32696, CECT 4026, CIP 103318, KCTC 3527, LMG 18297, LMG 9850, NRIC 1778, VTT E-98972

Strain history: CIP <- 1989, NCFB <- 1988, Shaw: strain SMl9, 1a21

NCBI tax ID(s): 927691 (strain), 1244 (species)

SI-ID 3491

LMG 9850, ATCC 49366, CCUG 30198, CCUG 32249, LMG 18297, NCFB 2775, CCUG 30062, CCUG 32696, CIP 103318, DSM 5578, JCM 9697, CECT 4026, NCIMB 12897, CCM 4189, KCTC 3527, VTT E-98972

Other strains from Leuconostoc gelidum

L. gelidum JCM 10094
L. gelidum JCM 10095
L. gelidum JCM 10096
L. gelidum JCM 10097
L. gelidum JCM 10098

Section

Leuconostoc gelidum SML9 is an anaerobe, mesophilic bacterium that was isolated from vakuum-packaged beef.

anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Leuconostoc*
	Species	**Leuconostoc gelidum**
	Full Scientific Name (LPSN)	Leuconostoc gelidum Shaw and Harding 1989

Information on morphological and physiological properties Morphology

[Ref.: #119567]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #119567]	Cell shape	oval-shaped

[Ref.: #119567]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %97.018

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2230]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #2230]

Culture medium growth

[Ref.: #2230]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2230]

Culture medium composition

expand / minimize

[Ref.: #2230]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #2230]

Culture medium growth

[Ref.: #2230]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2230]

Culture medium composition

expand / minimize

[Ref.: #36378]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #36378]

Culture medium growth

[Ref.: #36378]

Culture medium composition

expand / minimize

[Ref.: #119567]

Culture medium

CIP Medium 40

[Ref.: #119567]

Culture medium growth

[Ref.: #119567]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #2230]		growth	25 °C
[Ref.: #36378]		growth	25 °C
[Ref.: #67770]		growth	25 °C
[Ref.: #119567]		growth	10-25 °C
[Ref.: #119567]	no	growth	30 °C
[Ref.: #119567]	no	growth	37 °C
[Ref.: #119567]	no	growth	45 °C

[Ref.: #2230]	Temperature range	mesophilic
[Ref.: #36378]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #119567]	Temperature range	psychrophilic
[Ref.: #119567]	Temperature range	mesophilic
[Ref.: #119567]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #2230]	Oxygen tolerance	anaerobe
[Ref.: #119567]	Oxygen tolerance	facultative anaerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %100

[Ref.: #2230]	Murein short key	A11.05
[Ref.: #2230]	Murein types	A3alpha L-Lys-L-Ala₂

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #119567]	nitrate ChEBI	-	reduction
[Ref.: #119567]	nitrate ChEBI	+	respiration
[Ref.: #119567]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #119567]		acetoin ChEBI	-
[Ref.: #119567]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #119567]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #119567]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #119567]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119567]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #119567]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #2230]	14231	-	-	-	-	+	+	+	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	+	-	+	-	-	+	+	+	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119567]	7497	-	-	+	-	-	+	-	-	-	-	+	+	+	+	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2230]	Sample type/isolated from	vakuum-packaged beef

[Ref.: #67770]	Sample type/isolated from	Vacuum-packed meats

[Ref.: #119567]	Sample type/isolated from	Food, Vacuum packed meat
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Bottled, canned, packed food
#Engineered	#Food production	#Meat

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC096221 (>99% sequence identity) for Leuconostoc gelidum from Microbeatlas

Aquatic Samples	2226
Soil Samples	1578
Animal Samples	17034
Plant Samples	1382
Total Samples	22220

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2230]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119567]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #2230]	Leuconostoc gelidum 16S ribosomal RNA gene, partial sequence	AF175402	1505	ENA	927691 tax ID
[Ref.: #67770]	Leuconostoc gelidum subsp. gelidum gene for 16S ribosomal RNA, partial sequence, strain: JCM 9697	LC096221	1489	ENA	1607839 tax ID
[Ref.: #20218]	Leuconostoc gelidum gene for 16S rRNA, strain:NRIC 1778	AB022921	1445	ENA	927691 tax ID	*
[Ref.: #20218]	Leuconostoc gelidum DNA, 16S-23S rRNA intergenic spacer region, strain: NRIC 1778	AB290439	380	ENA	927691 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Leuconostoc gelidum subsp. gelidum KCTC 3527	GCA_000166715	contig	GenBank	927691 tax ID	*
[Ref.: #66792]	Leuconostoc gelidum KCTC 3527	927691.3	wgs	PATRIC	927691 tax ID	*
[Ref.: #66792]	Leuconostoc gelidum gelidum KCTC 3527	651324068	draft	JGI IMG/M	927691 tax ID	*

[Ref.: #2230]	GC-content	37.0 mol%
[Ref.: #67770]	GC-content	37 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Leuconostoc gelidum KCTC 3527 PATRIC: 927691.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Leuconostoc gelidum KCTC 3527 PATRIC: 927691.3
[Ref.: #69480]	motile	no	96.118	no
[Ref.: #69480]	flagellated	no	98.095	no
[Ref.: #69480]	gram-positive	yes	96.513	no
[Ref.: #69480]	anaerobic	no	91.278	no
[Ref.: #69480]	halophile	yes	95.403	no
[Ref.: #69480]	spore-forming	no	96.001	no
[Ref.: #69480]	glucose-util	yes	86.598	no
[Ref.: #69480]	aerobic	no	97.441	yes
[Ref.: #69480]	thermophile	no	99.848	yes
[Ref.: #69480]	glucose-ferment	yes	92.079	no

Availability in culture collections External links

[Ref.: #2230]

Culture collection no.

DSM 5578, ATCC 49366, NCFB 2775, NCIMB 12897, JCM 9697, CCM 4189, CCUG 30198, CCUG 32249, CCUG 32696, CECT 4026, CIP 103318, KCTC 3527, LMG 18297, LMG 9850, NRIC 1778, VTT E-98972

[Ref.: #76295]

SI-ID 3491

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Reclassification of Leuconostoc gasicomitatum as Leuconostoc gelidum subsp. gasicomitatum comb. nov., description of Leuconostoc gelidum subsp. aenigmaticum subsp. nov., designation of Leuconostoc gelidum subsp. gelidum subsp. nov. and emended description of Leuconostoc gelidum.	Rahkila R, De Bruyne K, Johansson P, Vandamme P, Bjorkroth J	Int J Syst Evol Microbiol	10.1099/ijs.0.058263-0	2014	*
Phylogeny	Leuconostoc miyukkimchii sp. nov., isolated from brown algae (Undaria pinnatifida) kimchi.	Lee SH, Park MS, Jung JY, Jeon CO	Int J Syst Evol Microbiol	10.1099/ijs.0.032367-0	2011	*
Genetics	Genome Sequence of Leuconostoc gelidum KCTC 3527, Isolated from Kimchi.	Kim DS, Choi SH, Kim DW, Kim RN, Nam SH, Kang A, Kim A, Park HS	J Bacteriol	10.1128/JB.01371-10	2010	*
Pathogenicity	Effect of Leuconostoc spp. on the formation of Streptococcus mutans biofilm.	Kang MS, Kang IC, Kim SM, Lee HC, Oh JS	J Microbiol	2570	2007	*
Enzymology	Characterization of a Leuconostoc gelidum bacteriophage from pork.	Greer GG, Dilts BD, Ackermann HW	Int J Food Microbiol	10.1016/j.ijfoodmicro.2006.09.021	2007	*
Phylogeny	Leuconostoc kimchii sp. nov., a new species from kimchi.	Kim J, Chun J, Han HU	Int J Syst Evol Microbiol	10.1099/00207713-50-5-1915	2000	*

References

#2230

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 5578

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#36378 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76295

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID3491.1 )

#119567 Collection of Institut Pasteur ; Curators of the CIP; CIP 103318
* These data were automatically processed and therefore are not curated

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Search for species Leuconostoc gelidum subsp. gelidum in external resources:
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Leuconostoc gelidum KCTC 3527

Leuconostoc gelidum KCTC 3527

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Change proposal