Name: Legionella pneumophila Philadelphia-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6712

Type strain:

Species: Legionella pneumophila

Strain Designation: Philadelphia-1

Culture col. no.: DSM 7513, ATCC 33152, WDCM 00107, CCUG 9568, NCTC 11192, JCM 7571, BCRC 17854, CECT 7109, CIP 103854, CCM 7799, Philadelphia 1

Strain history: CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- R.E. Weaver, CDC: strain Philadelphia 1 <- J.E. McDade

NCBI tax ID(s): 1236535 (strain), 446 (species)

SI-ID 40359

ATCC 33152, CCUG 9568, NCTC 11192, CIP 103854, DSM 7513, JCM 7571, KCTC 2614, KCTC 12009, ACM 5197, CECT 7109, CNCTC 7371, CCM 7799

Other strains from Legionella pneumophila

Section

Legionella pneumophila Philadelphia-1 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from human lung tissue.

Gram-negative
rod-shaped
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Legionellales*
	Family	*Legionellaceae*
	Genus	*Legionella*
	Species	**Legionella pneumophila**
	Full Scientific Name (LPSN)	Legionella pneumophila Brenner et al. 1979 (Approved Lists 1980)

Information on morphological and physiological properties Morphology

[Ref.: #121898]	Gram stain	negative

[Ref.: #121898]	Cell shape	rod-shaped

[Ref.: #121898]	Motility	no

[Ref.: #3193]

Incubation period

2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3193]

Culture medium

BCYE-AGAR (DSMZ Medium 585)

[Ref.: #3193]

Culture medium growth

[Ref.: #3193]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3193]

Culture medium composition

expand / minimize

[Ref.: #40120]

Culture medium

MEDIUM 23 - for Afipia and Legionella

[Ref.: #40120]

Culture medium growth

[Ref.: #40120]

Culture medium composition

expand / minimize

[Ref.: #121898]

Culture medium

CIP Medium 72

[Ref.: #121898]

Culture medium growth

[Ref.: #121898]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #121898]

Culture medium

CIP Medium 23

[Ref.: #121898]

Culture medium growth

[Ref.: #121898]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #3193]	growth	37 °C
[Ref.: #40120]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #3193]	Temperature range	mesophilic
[Ref.: #40120]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #3193]	Oxygen tolerance	microaerophile
[Ref.: #45204]	Oxygen tolerance	microaerophile

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68377]		tryptophan ChEBI	-	energy source	* from API NH

	Metabolite production	Metabolite	Production
[Ref.: #68377]		indole ChEBI	no	* from API NH

	Physiological tests	Metabolite	Indole test
[Ref.: #68377]		indole ChEBI	-	* from API NH

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API NH
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #3193]	catalase	+	1.11.1.6
[Ref.: #121898]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #3193]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121898]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68377]	tryptophan deaminase	-	4.1.99.1	* from API NH
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #45204]

Fatty acid	Percentage	ECL
C16:1 ω7c	29.6	15.819
C16:0 iso	23.4	15.626
C16:0	12	16
C15:0 ANTEISO	9.4	14.711
C17:0 anteiso	7.4	16.722
C16:1 ISO H	4.9	15.461
C18:0	3	18
C14:0 ISO	2.4	13.618
C20:0	1.9	20
C15:1 ω6c	1.5	14.856
C17:1 ω9c ANTEISO	1.4	16.525
C17:0	0.9	17
C14:0 ISO 3OH	0.8	15.117
C14:1 ω5c	0.7	13.899
C15:0	0.6	15

Metadata FA analysis

Reference

[Ref.: #45204]

Fatty acid	Percentage	ECL
C16:1 ω7c	35.3	15.819
C16:0 iso	17.8	15.626
C16:0	9.8	16
C15:0 ANTEISO	9.2	14.711
C14:0 ISO	5	13.618
C17:0 anteiso	3.4	16.722
C15:1 ω6c	3.3	14.856
C16:1 ISO H	2.7	15.461
C18:0	2.3	18
C20:0	2.2	20
C14:1 ω5c	1.8	13.899
C14:0	1.5	14
C14:0 ISO 3OH	1.3	15.117
C17:0 CYCLO	1.2	16.888
C15:0	1.1	15
Unidentified	0.9	16.318
C17:0	0.7	17
C19:0	0.5	19
Unidentified	0.4	13.244

Metadata FA analysis

Reference

[Ref.: #45204]

Fatty acid	Percentage	ECL
C16:1 ω7c	26.9	15.819
C16:0 iso	26.5	15.626
C15:0 ANTEISO	11	14.711
C14:0 ISO	7	13.618
C16:0	5.7	16
C17:0 anteiso	4.2	16.722
C16:1 ISO H	4.1	15.461
C15:1 ω6c	3.8	14.856
C18:0	1.9	18
C17:0 CYCLO	1.6	16.888
C20:0	1.6	20
C14:1 ω5c	1.3	13.899
C14:0 ISO 3OH	1	15.117
C17:0	1	17
C15:0	0.9	15
C19:0	0.5	19
C14:0	0.5	14
C17:1 ω9c ANTEISO	0.5	16.525

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #121898]	505	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® NH:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Penicillinase productionPEN	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from maltoseMAL	Acid from sucroseSAC	Ornithine decarboxylaseODC	Urease/urea hydrolysisURE	LipaseLIP	Alkaline phosphatasePAL	beta-Galactosidasebeta GAL	Proline arylamidaseProA	gamma-Glutamyl transferaseGGT	Indole productionIND
[Ref.: #3193]	6013	-	-	-	-	-	-	+/-	+	-	-	+	-	-
[Ref.: #3193]	6012	+	+	+	+	+/-	-	-	+	-	-	+	-	-
[Ref.: #3193]	6011	+	+/-	-	-	-	-	+	+	-	-	+	-	-
[Ref.: #3193]	5862	-	+	+/-	-	-	-	-	+	+	-	-	+	-
[Ref.: #3193]	5861	-	+	+	+	+	+/-	-	-	+	-	-	+	-
[Ref.: #3193]	5860	-	-	-	-	-	-	-	+/-	+	-	-	+	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121898]	7702	-	+	+	+	-	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3193]	Sample type/isolated from	human lung tissue
[Ref.: #3193]	Geographic location (country and/or sea, region)	Philadelphia
[Ref.: #3193]	Country	USA
[Ref.: #3193]	Country ISO 3 Code	USA
[Ref.: #3193]	Continent	North America
[Ref.: #3193]	Geographic location	39.9526°/-75.1652°

[Ref.: #45204]	Sample type/isolated from	Human lung
[Ref.: #45204]	Geographic location (country and/or sea, region)	Philadelphia
[Ref.: #45204]	Country	USA
[Ref.: #45204]	Country ISO 3 Code	USA
[Ref.: #45204]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Human lung

[Ref.: #121898]	Sample type/isolated from	Human, Lung
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body-Site	#Oral cavity and airways	#Lung
#Infection	#Patient	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB594755 (>99% sequence identity) for Legionella pneumophila subclade from Microbeatlas

Aquatic Samples	6971
Soil Samples	6537
Animal Samples	2429
Plant Samples	1018
Total Samples	16955

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3193]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #121898]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Legionella pneumophila subsp. pneumophila gene for 16S rRNA, partial sequence, strain: JCM 7571	AB594755	1466	ENA	91891 tax ID
[Ref.: #67770]	Legionella pneumophila strain Philadelphia 1 16S ribosomal RNA gene, partial sequence	M36023	1474	ENA	446 tax ID
[Ref.: #20218]	Legionella pneumophila strain ATCC33152 16S-23S rRNA intergenic region with tRNA-Ala-like sequence	AF000655	448	ENA	446 tax ID	*
[Ref.: #20218]	Legionella pneumophila 16S ribosomal RNA	M59157	1544	ENA	446 tax ID	*
[Ref.: #20218]	L.pneumophila (strain Philadelphia-1) 16S rRNA, partial, segment 2 of 3	M19444	137	ENA	446 tax ID	*
[Ref.: #20218]	L.pneumophila (strain Philadelphia-1) 16S rRNA, partial, segment 1 of 3	M19450	125	ENA	446 tax ID	*
[Ref.: #20218]	L.pneumophila (strain Philadelphia-1) 16S rRNA, partial, segment 3 of 3	M22266	141	ENA	446 tax ID	*
[Ref.: #20218]	Legionella pneumophila 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer and tRNA-Ala gene, complete sequence; and 23S ribosomal RNA gene, partial sequence	AY298787	3248	ENA	446 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Legionella pneumophila subsp. pneumophila str. Philadelphia 1	GCA_000008485	complete	GenBank	272624 tax ID
[Ref.: #66792]	Legionella pneumophila NCTC11192	GCA_900452735	contig	GenBank	446 tax ID	*
[Ref.: #66792]	Legionella pneumophila ATCC 33152	GCA_001582475	scaffold	GenBank	446 tax ID	*
[Ref.: #66792]	Legionella pneumophila subsp. pneumophila ATCC 33152	GCA_000586375	scaffold	GenBank	91891 tax ID	*
[Ref.: #66792]	Legionella pneumophila subsp. pneumophila JCM 7571	GCA_000614785	contig	GenBank	1236535 tax ID	*
[Ref.: #66792]	Legionella pneumophila strain ATCC 33152	446.161	wgs	PATRIC	446 tax ID	*

[Ref.: #3193]

GC-content

39.0 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Legionella pneumophila Philadelphia-1 NCBI: GCA_001941585.1
[Ref.: #69480]	flagellated	no	68.906	no
[Ref.: #69480]	gram-positive	no	98.641	no
[Ref.: #69480]	anaerobic	no	98.672	yes
[Ref.: #69480]	halophile	no	88.876	no
[Ref.: #69480]	spore-forming	no	94.592	no
[Ref.: #69480]	glucose-util	yes	69.005	no
[Ref.: #69480]	aerobic	no	91.407	yes
[Ref.: #69480]	thermophile	no	98.315	yes
[Ref.: #69480]	motile	yes	80.696	no
[Ref.: #69480]	glucose-ferment	no	85.308	no

Availability in culture collections External links

[Ref.: #3193]

Culture collection no.

DSM 7513, ATCC 33152, WDCM 00107, CCUG 9568, NCTC 11192, JCM 7571, BCRC 17854, CECT 7109, CIP 103854, CCM 7799, Philadelphia 1

[Ref.: #76185]

SI-ID 40359

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Novel axially symmetric and unsymmetric silicon(IV) phthalocyanines having anti-inflammatory groups: synthesis, characterization and their biological properties.	Gumusgoz Celik G, Gonca S, Sahin B, Ozdemir S, Atilla D, Gurek AG	Dalton Trans	10.1039/d2dt00652a	2022	*
Pathogenicity	A new MALDI-TOF approach for the quick sequence type identification of Legionella pneumophila.	Blanco S, Sanz C, Gutierrez MP, Simarro M, Lopez I, Escribano I, Eiros JM, Zarzosa P, Orduna A, Lopez JC, March GA	J Microbiol Methods	10.1016/j.mimet.2021.106292	2021	*
Stress	The Tail-Specific Protease Is Important for Legionella pneumophila To Survive Thermal Stress in Water and inside Amoebae.	Saoud J, Mani T, Faucher SP	Appl Environ Microbiol	10.1128/AEM.02975-20	2021	*
Cultivation	Legionella Detection in Water Networks as per ISO 11731:2017: Can Different Filter Pore Sizes and Direct Placement on Culture Media Influence Laboratory Results?	De Giglio O, Diella G, Trerotoli P, Consonni M, Palermo R, Tesauro M, Lagana P, Serio G, Montagna MT	Int J Environ Res Public Health	10.3390/ijerph17062077	2020	*
Pathogenicity	Divergent Evolution of Legionella RCC1 Repeat Effectors Defines the Range of Ran GTPase Cycle Targets.	Swart AL, Steiner B, Gomez-Valero L, Schutz S, Hannemann M, Janning P, Irminger M, Rothmeier E, Buchrieser C, Itzen A, Panse VG, Hilbi H	mBio	10.1128/mBio.00405-20	2020	*
Pathogenicity	Antibiotic Resistance and Azithromycin Resistance Mechanism of Legionella pneumophila Serogroup 1 in China.	Jia X, Ren H, Nie X, Li Y, Li J, Qin T	Antimicrob Agents Chemother	10.1128/AAC.00768-19	2019	*
Pathogenicity	Legionella pneumophila p45 element influences host cell entry and sensitivity to sodium.	Christensen LM, Sule P, Strain M, Cirillo JD	PLoS One	10.1371/journal.pone.0218941	2019	*
Pathogenicity	Chlorine and Monochloramine Disinfection of Legionella pneumophila Colonizing Copper and Polyvinyl Chloride Drinking Water Biofilms.	Buse HY, J Morris B, Struewing IT, Szabo JG	Appl Environ Microbiol	10.1128/AEM.02956-18	2019	*
Pathogenicity	Using an optimal set of features with a machine learning-based approach to predict effector proteins for Legionella pneumophila.	Esna Ashari Z, Brayton KA, Broschat SL	PLoS One	10.1371/journal.pone.0202312	2019	*
Metabolism	An investigation of virulence factors of Legionella pneumophila environmental isolates.	Arslan-Aydogdu EO, Kimiran A	Braz J Microbiol	10.1016/j.bjm.2017.03.012	2017	*
Stress	Resistance of Legionella and Acanthamoeba mauritaniensis to heat treatment as determined by relative and quantitative polymerase chain reactions.	Dobrowsky PH, Khan S, Khan W	Environ Res	10.1016/j.envres.2017.06.003	2017	*
Genetics	Draft Genome Sequences of Legionella pneumophila JR32 and Lp01 Laboratory Strains Domesticated in Japan.	Maita C, Matushita M, Okubo T, Matsuo J, Miyake M, Nagai H, Yamaguchi H	Genome Announc	10.1128/genomeA.00791-16	2016	*
Metabolism	Iron Limitation Triggers Early Egress by the Intracellular Bacterial Pathogen Legionella pneumophila.	O'Connor TJ, Zheng H, VanRheenen SM, Ghosh S, Cianciotto NP, Isberg RR	Infect Immun	10.1128/IAI.01306-15	2016	*
Pathogenicity	Effects of oakmoss and its components on Acanthamoeba castellanii ATCC 30234 and the uptake of Legionella pneumophila JCM 7571 (ATCC 33152) into A. castellanii.	Nomura H, Isshiki Y, Sakuda K, Sakuma K, Kondo S	Biocontrol Sci	10.4265/bio.20.59	2015	*
Phylogeny	Legionella norrlandica sp. nov., isolated from the biopurification systems of wood processing plants.	Rizzardi K, Winiecka-Krusnell J, Ramliden M, Alm E, Andersson S, Byfors S	Int J Syst Evol Microbiol	10.1099/ijs.0.068940-0	2014	*
Metabolism	The novel Legionella pneumophila type II secretion substrate NttC contributes to infection of amoebae Hartmannella vermiformis and Willaertia magna.	Tyson JY, Vargas P, Cianciotto NP	Microbiology (Reading)	10.1099/mic.0.082750-0	2014	*
Pathogenicity	Legionella in water samples: how can you interpret the results obtained by quantitative PCR?	Ditommaso S, Ricciardi E, Giacomuzzi M, Arauco Rivera SR, Zotti CM	Mol Cell Probes	10.1016/j.mcp.2014.09.002	2014	*
Metabolism	Hartmannella vermiformis inhibition of Legionella pneumophila cultivability.	Buse HY, Donohue MJ, Ashbolt NJ	Microb Ecol	10.1007/s00248-013-0250-z	2013	*
Metabolism	Implication of the VirD4 coupling protein of the Lvh type 4 secretion system in virulence phenotypes of Legionella pneumophila.	Bandyopadhyay P, Lang EA, Rasaputra KS, Steinman HM	J Bacteriol	10.1128/JB.00430-13	2013	*
Phylogeny	Phylogenetic reconstruction of the Legionella pneumophila Philadelphia-1 laboratory strains through comparative genomics.	Rao C, Benhabib H, Ensminger AW	PLoS One	10.1371/journal.pone.0064129	2013	*
Pathogenicity	Assessment of cytokine release after in vitro stimulation of whole blood with legionella pneumophila in immunocompromised patients.	Sikora A, Koziol-Montewka M, Ksiazek A, Wojtowicz M, Paluch-Oles J, Magrys A, Grzebalska A, Bednarek-Skublewska A, Stec A, Rudzki S, Furmaga J, Matuszewska R, Krogulska B	Immunol Invest	10.3109/08820139.2012.719562	2013	*
Pathogenicity	Protozoa and human macrophages infection by Legionella pneumophila environmental strains belonging to different serogroups.	Messi P, Bargellini A, Anacarso I, Marchesi I, de Niederhausern S, Bondi M	Arch Microbiol	10.1007/s00203-012-0851-9	2012	*
Phenotype	Virulence phenotypes of Legionella pneumophila associated with noncoding RNA lpr0035.	Jayakumar D, Early JV, Steinman HM	Infect Immun	10.1128/IAI.00598-12	2012	*
Pathogenicity	Detection of Legionella pneumophila on clinical samples and susceptibility assessment by flow cytometry.	Faria-Ramos I, Costa-de-Oliveira S, Barbosa J, Cardoso A, Santos-Antunes J, Rodrigues AG, Pina-Vaz C	Eur J Clin Microbiol Infect Dis	10.1007/s10096-012-1702-y	2012	*
Genetics	Liver cirrhosis as a predisposing condition for Legionnaires' disease: a report of four laboratory-confirmed cases from China.	Qin T, Xia J, Ren H, Zhou H, Tang B, Shao Z	J Med Microbiol	10.1099/jmm.0.040170-0	2012	*
Phylogeny	Evaluation of DNA isolation procedures for detecting and quantifying environmental Legionella by real-time quantitative PCR.	Chen NT, Chang CW, Wu JD	Water Sci Technol	10.2166/wst.2012.001	2012	*
Genetics	The N-acylneuraminate cytidyltransferase gene, neuA, is heterogenous in Legionella pneumophila strains but can be used as a marker for epidemiological typing in the consensus sequence-based typing scheme.	Farhat C, Mentasti M, Jacobs E, Fry NK, Luck C	J Clin Microbiol	10.1128/JCM.00687-11	2011	*
Pathogenicity	Differential growth of Legionella pneumophila strains within a range of amoebae at various temperatures associated with in-premise plumbing.	Buse HY, Ashbolt NJ	Lett Appl Microbiol	10.1111/j.1472-765X.2011.03094.x	2011	*
Enzymology	Genomic diversity of Legionella pneumophila serogroup 1 from environmental water sources and clinical specimens using pulsed-field gel electrophoresis (PFGE) from 1985 to 2007, Korea.	Lee HK, Kang YH, Yu JY	J Microbiol	10.1007/s12275-010-0031-z	2010	*
Genetics	Legionella pneumophila strain 130b possesses a unique combination of type IV secretion systems and novel Dot/Icm secretion system effector proteins.	Schroeder GN, Petty NK, Mousnier A, Harding CR, Vogrin AJ, Wee B, Fry NK, Harrison TG, Newton HJ, Thomson NR, Beatson SA, Dougan G, Hartland EL, Frankel G	J Bacteriol	10.1128/JB.00778-10	2010	*
Pathogenicity	In vitro activity of antimicrobial agents against Legionella isolated from environmental water systems: first results from Turkey.	Erdogan H, Can F, Demirbilek M, Timurkaynak F, Arslan H	Environ Monit Assess	10.1007/s10661-009-1293-y	2010	*
Phylogeny	Legionella dresdenensis sp. nov., isolated from river water.	Luck PC, Jacobs E, Roske I, Schroter-Bobsin U, Dumke R, Gronow S	Int J Syst Evol Microbiol	10.1099/ijs.0.017863-0	2009	*
Phylogeny	[Comparative study of the biological properties of various nutrient media used for the isolation of Legionella pneumophila].	Shelokhovich AI, Kharabadzhakhian GD, Karbyshev GL, Terent'ev AN, Savel'eva IK, Savel'eva EP, Sokirkina OG	Klin Lab Diagn		2009	*
Pathogenicity	Autophagy induced by 2-deoxy-D-glucose suppresses intracellular multiplication of Legionella pneumophila in A/J mouse macrophages.	Matsuda F, Fujii J, Yoshida S	Autophagy	10.4161/auto.5.4.7760	2009	*
Phylogeny	[Molecular genetic typing of Legionella pneumophila serogroup 1 isolated in town Verkhnyaya Pyshma using international standards].	Voronina OL, Lunin VG	Zh Mikrobiol Epidemiol Immunobiol		2008	*
Metabolism	Lgt: a family of cytotoxic glucosyltransferases produced by Legionella pneumophila.	Belyi Y, Tabakova I, Stahl M, Aktories K	J Bacteriol	10.1128/JB.01798-07	2008	*
Metabolism	Differential 2-D protein gel electrophoresis analysis of Legionella pneumophila wild type and Tat secretion mutants.	De Buck E, Hoper D, Lammertyn E, Hecker M, Anne J	Int J Med Microbiol	10.1016/j.ijmm.2007.06.003	2007	*
Metabolism	The twin-arginine translocation pathway is necessary for correct membrane insertion of the Rieske Fe/S protein in Legionella pneumophila.	De Buck E, Vranckx L, Meyen E, Maes L, Vandersmissen L, Anne J, Lammertyn E	FEBS Lett	10.1016/j.febslet.2006.12.022	2006	*
Pathogenicity	Distribution of 19 major virulence genes in Legionella pneumophila serogroup 1 isolates from patients and water in Queensland, Australia.	Huang B, Yuan Z, Heron BA, Gray BR, Eglezos S, Bates JR, Savill J	J Med Microbiol	10.1099/jmm.0.46310-0	2006	*
Metabolism	Evidence for acquisition of Legionella type IV secretion substrates via interdomain horizontal gene transfer.	de Felipe KS, Pampou S, Jovanovic OS, Pericone CD, Ye SF, Kalachikov S, Shuman HA	J Bacteriol	10.1128/JB.187.22.7716-7726.2005	2005	*
Pathogenicity	Legionella pneumophila Philadelphia-1 tatB and tatC affect intracellular replication and biofilm formation.	De Buck E, Maes L, Meyen E, Van Mellaert L, Geukens N, Anne J, Lammertyn E	Biochem Biophys Res Commun	10.1016/j.bbrc.2005.04.060	2005	*
Enzymology	First proteomic analysis of Legionella pneumophila based on its developing genome sequence.	Lebeau I, Lammertyn E, De Buck E, Maes L, Geukens N, Van Mellaert L, Arckens L, Anne J, Clerens S	Res Microbiol	10.1016/j.resmic.2004.08.010	2005	*
Pathogenicity	Expression of magA in Legionella pneumophila Philadelphia-1 is developmentally regulated and a marker of formation of mature intracellular forms.	Hiltz MF, Sisson GR, Brassinga AK, Garduno E, Garduno RA, Hoffman PS	J Bacteriol	10.1128/JB.186.10.3038-3045.2004	2004	*
Pathogenicity	Virulence conversion of Legionella pneumophila by conjugal transfer of chromosomal DNA.	Miyamoto H, Yoshida S, Taniguchi H, Shuman HA	J Bacteriol	10.1128/JB.185.22.6712-6718.2003	2003	*
Pathogenicity	A 65-kilobase pathogenicity island is unique to Philadelphia-1 strains of Legionella pneumophila.	Brassinga AK, Hiltz MF, Sisson GR, Morash MG, Hill N, Garduno E, Edelstein PH, Garduno RA, Hoffman PS	J Bacteriol	10.1128/JB.185.15.4630-4637.2003	2003	*
Genetics	Characterization of a tandem repeat polymorphism in Legionella pneumophila and its use for genotyping.	Pourcel C, Vidgop Y, Ramisse F, Vergnaud G, Tram C	J Clin Microbiol	10.1128/JCM.41.5.1819-1826.2003	2003	*
Genetics	Sequence and genome context analysis of a new molecular class D beta-lactamase gene from Legionella pneumophila.	Avison MB, Simm AM	J Antimicrob Chemother	10.1093/jac/dkf135	2002	*
Pathogenicity	Intracellular activity of ABT-773 and other antimicrobial agents against Legionella pneumophila.	Jung R, Danziger LH, Pendland SL	J Antimicrob Chemother	10.1093/jac/dkf017	2002	*
Pathogenicity	Morphological variety of intracellular microcolonies of Legionella species in Vero cells.	Ogawa M, Takade A, Miyamoto H, Taniguchi H, Yoshida S	Microbiol Immunol	10.1111/j.1348-0421.2001.tb02658.x	2001	*
Stress	Disinfectant effects of hot water, ultraviolet light, silver ions and chlorine on strains of Legionella and nontuberculous mycobacteria.	Miyamoto M, Yamaguchi Y, Sasatsu M	Microbios		2000	*
Metabolism	Discovery of a nonclassical siderophore, legiobactin, produced by strains of Legionella pneumophila.	Liles MR, Scheel TA, Cianciotto NP	J Bacteriol	10.1128/JB.182.3.749-757.2000	2000	*
Pathogenicity	Grouping of 20 reference strains of Legionella species by the growth ability within mouse and guinea pig macrophages.	Izu K, Yoshida S, Miyamoto H, Chang B, Ogawa M, Yamamoto H, Goto Y, Taniguchi H	FEMS Immunol Med Microbiol	10.1111/j.1574-695X.1999.tb01372.x	1999	*
Enzymology	Increased plasma adenosine deaminase activity in the early phase of Legionella pneumophila infection in guinea pigs.	Nikaido Y, Nagata N, Kido M, Yoshida S	FEMS Immunol Med Microbiol	10.1111/j.1574-695X.1996.tb00265.x	1996	*
Pathogenicity	Enzyme immunoassay for detection of antibodies against isolated flagella of Legionella pneumophila.	Neumeister B, Susa M, Nowak B, Straube E, Ruckdeschel G, Hacker J, Marre R	Eur J Clin Microbiol Infect Dis	10.1007/BF01690990	1995	*
Enzymology	Protein profiles of Legionella pneumophila Philadelphia-1 grown in macrophages and characterization of a gene encoding a novel 24 kDa Legionella protein.	Miyamoto H, Yoshida S, Taniguchi H, Qin MH, Fujio H, Mizuguchi Y	Microb Pathog	10.1006/mpat.1993.1095	1993	*
Pathogenicity	[Study of Legionella pneumophila detection by two step polymerase chain reaction].	Koide M, Saito A, Higa F, Yamashiro Y, Ishimine T, Futenma M, Inadome J, Kawakami K, Kusano N	Kansenshogaku Zasshi	10.11150/kansenshogakuzasshi1970.67.1062	1993	*
Phylogeny	Isolation and characterization of a lipopolysaccharide mutant of Legionella pneumophila.	Mintz CS, Zou CH	FEMS Microbiol Lett	10.1016/0378-1097(92)90470-9	1992	*
Phylogeny	Genetic control of natural resistance in mouse macrophages regulating intracellular Legionella pneumophila multiplication in vitro.	Yoshida S, Goto Y, Mizuguchi Y, Nomoto K, Skamene E	Infect Immun	10.1128/iai.59.1.428-432.1991	1991	*
Enzymology	[Specific DNA probe for detecting Legionella pneumophila].	Nagaev IG, Zigangirova NA, Gintsburg AL, Tartakovskii IS, Prozorovskii SV	Mol Gen Mikrobiol Virusol		1990	*
Pathogenicity	The HL-60 model for the interaction of human macrophages with the Legionnaires' disease bacterium.	Marra A, Horwitz MA, Shuman HA	J Immunol		1990	*
Phylogeny	Isolation of a Legionella pneumophila restriction mutant with increased ability to act as a recipient in heterospecific matings.	Marra A, Shuman HA	J Bacteriol	10.1128/jb.171.4.2238-2240.1989	1989	*
Pathogenicity	Isolation and characterization of auxotrophic mutants of Legionella pneumophila that fail to multiply in human monocytes.	Mintz CS, Chen JX, Shuman HA	Infect Immun	10.1128/iai.56.6.1449-1455.1988	1988	*
Pathogenicity	Effect of quinolones and other antimicrobial agents on cell-associated Legionella pneumophila.	Havlichek D, Saravolatz L, Pohlod D	Antimicrob Agents Chemother	10.1128/AAC.31.10.1529	1987	*
Metabolism	Fate of Legionella pneumophila Philadelphia-1 strain in resident, elicited, activated, and immune peritoneal macrophages of guinea pigs.	Yoshida S, Mizuguchi Y, Nikaido Y, Mitsuyama M, Nomoto K	Infect Immun	10.1128/iai.55.10.2477-2482.1987	1987	*
Pathogenicity	In vitro effects of monobactams on Legionella pneumophila.	Yoshida S, Mizuguchi Y, Nikaido Y	J UOEH	10.7888/juoeh.9.299	1987	*
Enzymology	[Extracellular metalloproteinase from Legionella pneumophila].	Gul'nik SV, Lavrenova GI, Stepanov VM	Biokhimiia		1987	*
Enzymology	[Doxycycline treatment of experimental legionellosis in guinea pigs infected by aerosol].	Nowicki M, Paucod JC, Bornstein N, Meugnier H, Freney J, Isoard P, Fleurette J	Pathol Biol (Paris)		1987	*
Pathogenicity	Effects of tetracyclines on experimental Legionella pneumophila infection in guinea-pigs.	Yoshida S, Mizuguchi Y, Ohta H, Ogawa M	J Antimicrob Chemother	10.1093/jac/16.2.199	1985	*
Phylogeny	DNA probe specific for Legionella pneumophila.	Grimont PA, Grimont F, Desplaces N, Tchen P	J Clin Microbiol	10.1128/jcm.21.3.431-437.1985	1985	*
Pathogenicity	Antibiotic susceptibility of Legionella pneumophia Philadelphia-1 in cultured guinea-pig peritoneal macrophages.	Yoshida S, Mizuguchi Y	J Gen Microbiol	10.1099/00221287-130-4-901	1984	*
	Re-opening hairdressing salons, barber shops and gyms following COVID-19 lockdown: reducing risks from Legionella species through successful domestic steam disinfection of showerheads.	Millar BC, Ferris J, Murphy A, Reid N, Moore JE	Access Microbiol	10.1099/acmi.0.000229	2021	*
	[Investigation of the Incidence of Legionella and Free-Living Amoebae in Swimming Pool Waters and Biofilm Specimens in Istanbul by Different Methods].	Zeybek Z, Turkmen A	Mikrobiyol Bul	10.5578/mb.68962	2020	*
	Convolutional neural network-based annotation of bacterial type IV secretion system effectors with enhanced accuracy and reduced false discovery.	Hong J, Luo Y, Mou M, Fu J, Zhang Y, Xue W, Xie T, Tao L, Lou Y, Zhu F	Brief Bioinform	10.1093/bib/bbz120	2020	*
	Genomic Analysis Reveals Novel Diversity among the 1976 Philadelphia Legionnaires' Disease Outbreak Isolates and Additional ST36 Strains.	Mercante JW, Morrison SS, Desai HP, Raphael BH, Winchell JM	PLoS One	10.1371/journal.pone.0164074	2016	*
	Role of biofilm in protection of the replicative form of Legionella pneumophila.	Andreozzi E, Di Cesare A, Sabatini L, Chessa E, Sisti D, Rocchi M, Citterio B	Curr Microbiol	10.1007/s00284-014-0648-y	2014	*
	Influence of temperature on growth of Legionella pneumophila biofilm determined by precise temperature gradient incubator.	Konishi T, Yamashiro T, Koide M, Nishizono A	J Biosci Bioeng	10.1263/jbb.101.478	2006	*
	Fate of Legionella pneumophila in macrophages of C57BL/6 chronic granulomatous disease mice.	Saito M, Kajiwara H, Miyamoto H, Yoshida S	Microbiol Immunol	10.1111/j.1348-0421.2001.tb02655.x	2001	*
	Intracellular multiplication of Legionella pneumophila in Tetrahymena thermophila.	Kikuhara H, Ogawa M, Miyamoto H, Nikaido Y, Yoshida S	J UOEH	10.7888/juoeh.16.263	1994	*
	Multiplication of Legionella pneumophila Philadelphia-1 in cultured peritoneal macrophages and its correlation to susceptibility of animals.	Yoshida S, Mizuguchi Y	Can J Microbiol	10.1139/m86-083	1986	*
	A comparison of virulence of two strains of Legionella pneumophila based on experimental aerosol infection of guinea-pigs.	Jepras RI, Fitzgeorge RB, Baskerville A	J Hyg (Lond)	10.1017/s0022172400062252	1985	*
	Aerosol infection of animals with strains of Legionella pneumophila of different virulence: comparison with intraperitoneal and intranasal routes of infection.	Fitzgeorge RB, Baskerville A, Broster M, Hambleton P, Dennis PJ	J Hyg (Lond)	10.1017/s0022172400063877	1983	*

References

#3193

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7513

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40120 ; Curators of the CIP;
#45204 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 9568

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68377

Automatically annotated from API NH .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76185

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID40359.1 )

#121898 Collection of Institut Pasteur ; Curators of the CIP; CIP 103854
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Legionella pneumophila subsp. pneumophila in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF