Name: Limosilactobacillus mucosae S32
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6584

Type strain:

Species: Limosilactobacillus mucosae

Strain Designation: S32

Culture col. no.: DSM 13345, CCUG 43179, CIP 106485, JCM 12515, KCTC 21011, LMG 19534, NCIMB 13705

Strain history: CIP <- 2000, CCUG <- S. Roos, SLU, Swedish Univ. Agr. Sci., Uppsala, Sweden: strain S32

NCBI tax ID(s): 1423771 (strain), 97478 (species)

SI-ID 13438

LMG 19534, CCUG 43179, CIP 106485, DSM 13345, NCIMB 13705, JCM 12515

Other strains from Limosilactobacillus mucosae

L. mucosae S14, DSM 13346, CCUG 43180
L. mucosae MRS-253-APC-2B, DSM 102820
L. mucosae Cla-CZ-100, DSM 109049
L. mucosae JCM 7717
L. mucosae JCM 7735
L. mucosae JCM 7737
L. mucosae CRBIP24.8

Section

Limosilactobacillus mucosae S32 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from pig small intestine.

Gram-positive
rod-shaped
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Limosilactobacillus*
	Species	**Limosilactobacillus mucosae**
	Full Scientific Name (LPSN)	Limosilactobacillus mucosae (Roos et al. 2000) Zheng et al. 2020

Synonym

Lactobacillus mucosae

Information on morphological and physiological properties Morphology

[Ref.: #121164]	Gram stain	positive

[Ref.: #121164]	Cell shape	rod-shaped

[Ref.: #121164]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5007]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #5007]

Culture medium growth

[Ref.: #5007]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5007]

Culture medium composition

expand / minimize

[Ref.: #39538]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #39538]

Culture medium growth

[Ref.: #39538]

Culture medium composition

expand / minimize

[Ref.: #121164]

Culture medium

CIP Medium 40

[Ref.: #121164]

Culture medium growth

[Ref.: #121164]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5007]		growth	37 °C
[Ref.: #39538]		growth	37 °C
[Ref.: #55710]		growth	37 °C
[Ref.: #67770]		growth	37 °C
[Ref.: #121164]		growth	30-45 °C
[Ref.: #121164]	no	growth	10 °C
[Ref.: #121164]	no	growth	15 °C
[Ref.: #121164]	no	growth	22 °C

[Ref.: #5007]	Temperature range	mesophilic
[Ref.: #39538]	Temperature range	mesophilic
[Ref.: #55710]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #121164]	Temperature range	psychrophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #55710]

Oxygen tolerance

anaerobe

[Ref.: #5007]	Murein short key	A21.04
[Ref.: #5007]	Murein types	A4ß L-Orn-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121164]	nitrate ChEBI	-	reduction
[Ref.: #121164]	nitrate ChEBI	+	respiration
[Ref.: #121164]	nitrite ChEBI	-	reduction
[Ref.: #68371]	raffinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #121164]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #121164]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #121164]	alcohol dehydrogenase	+	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121164]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121164]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121164]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #121164]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #5007]	14304	-	-	-	-	-	-	+	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #5007]	14303	-	-	-	-	-	+	+	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	+	+
[Ref.: #5007]	14978	-	-	-	-	-	+	+	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121164]	9544	-	-	+	+	-	+	-	-	-	-	+	+	+	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5007]	Sample type/isolated from	pig small intestine
[Ref.: #5007]	Country	Sweden
[Ref.: #5007]	Country ISO 3 Code	SWE
[Ref.: #5007]	Continent	Europe

[Ref.: #55710]	Sample type/isolated from	Pig small intestine,newly slaughtered
[Ref.: #55710]	Sampling date	1997-09-01
[Ref.: #55710]	Geographic location (country and/or sea, region)	Uppsala
[Ref.: #55710]	Country	Sweden
[Ref.: #55710]	Country ISO 3 Code	SWE
[Ref.: #55710]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Small intestine of pig

[Ref.: #121164]	Sample type/isolated from	Animal, Pig, small intestine
[Ref.: #121164]	Geographic location (country and/or sea, region)	Uppsala
[Ref.: #121164]	Country	Sweden
[Ref.: #121164]	Country ISO 3 Code	SWE
[Ref.: #121164]	Continent	Europe
[Ref.: #121164]	Isolation date	1997
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Suidae (Pig,Swine)
#Host Body-Site	#Gastrointestinal tract	#Small intestine

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC383823 (>99% sequence identity) for Limosilactobacillus mucosae subclade from Microbeatlas

Aquatic Samples	7206
Soil Samples	2576
Animal Samples	94221
Plant Samples	1502
Total Samples	105505

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5007]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121164]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lactobacillus mucosae JCM 12515 gene for 16S ribosomal RNA, partial sequence	LC383823	1515	ENA	97478 tax ID
[Ref.: #5007]	Lactobacillus mucosae 16S ribosomal RNA gene, complete sequence	AF126738	1568	ENA	97478 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Limosilactobacillus mucosae DSM 13345	GCA_001436025	contig	GenBank	1423771 tax ID
[Ref.: #66792]	Lactobacillus mucosae DSM 13345	1423771.3	wgs	PATRIC	1423771 tax ID	*
[Ref.: #66792]	Limosilactobacillus mucosae DSM 13345	2657245411	draft	JGI IMG/M	1423771 tax ID	*

[Ref.: #5007]	GC-content	46 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	46.3-46.7 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	46.4 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lactobacillus mucosae DSM 13345 PATRIC: 1423771.3
[Ref.: #69480]	motile	no	97.029	no
[Ref.: #69480]	gram-positive	yes	96.744	no
[Ref.: #69480]	anaerobic	no	51.204	yes
[Ref.: #69480]	halophile	yes	88.702	no
[Ref.: #69480]	spore-forming	no	96.802	no
[Ref.: #69480]	thermophile	no	99.314	yes
[Ref.: #69480]	glucose-util	yes	89.856	no
[Ref.: #69480]	aerobic	no	97.571	yes
[Ref.: #69480]	flagellated	no	98.397	no
[Ref.: #69480]	glucose-ferment	yes	87.724	no

Availability in culture collections External links

[Ref.: #5007]

Culture collection no.

DSM 13345, CCUG 43179, CIP 106485, JCM 12515, KCTC 21011, LMG 19534, NCIMB 13705

[Ref.: #76058]

SI-ID 13438

Literature:

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Effects of the probiotic Enterococcus faecium NCIMB 10415 on selected lactic acid bacteria and enterobacteria in co-culture.	Starke IC, Zentek J, Vahjen W	Benef Microbes	10.3920/BM2014.0052	2015	*
Phylogeny	Development and assessment of a real-time pcr assay for rapid and sensitive detection of a novel thermotolerant bacterium, Lactobacillus thermotolerans, in chicken feces.	Selim AS, Boonkumklao P, Sone T, Assavanig A, Wada M, Yokota A	Appl Environ Microbiol	10.1128/AEM.71.8.4214-4219.2005	2005	*

References

#5007

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13345

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#39538 ; Curators of the CIP;
#55710 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 43179

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76058

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID13438.1 )

#121164 Collection of Institut Pasteur ; Curators of the CIP; CIP 106485
* These data were automatically processed and therefore are not curated

Change proposal

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Lactobacillus mucosae DSM 13345

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