Name: Chryseobacterium bovis H9
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5691

Type strain:

Species: Chryseobacterium bovis

Strain Designation: H9

Culture col. no.: DSM 19482, LMG 24227, CIP 110170

Strain history: CIP <- 2010, DSMZ <- M. Halpern, Haifa Univ., Haifa, Israel: strain H9 <- E. Hantsis-Zacharov and M. Halpern

NCBI tax ID(s): 421530 (species)

SI-ID 343788

DSM 19482, LMG 24227, CIP 110170

Other strains from Chryseobacterium bovis

C. bovis H15, DSM 19481
C. bovis H10, DSM 19483

Section

Chryseobacterium bovis H9 is a facultative aerobe, mesophilic, Gram-negative bacterium that was isolated from raw cow’s milk.

Gram-negative
rod-shaped
facultative aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Weeksellaceae*
	Genus	*Chryseobacterium*
	Species	**Chryseobacterium bovis**
	Full Scientific Name (LPSN)	Chryseobacterium bovis Hantsis-Zacharov et al. 2008

Synonym

Epilithonimonas bovis

Information on morphological and physiological properties Morphology

[Ref.: #32439]	Gram stain	negative
[Ref.: #116910]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.984

[Ref.: #32439]	Cell length	1.7 µm

[Ref.: #32439]	Cell width	0.7 µm

[Ref.: #32439]	Cell shape	rod-shaped
[Ref.: #116910]	Cell shape	rod-shaped

[Ref.: #32439]	Motility	no
[Ref.: #116910]	Motility	no

[Ref.: #32439]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8044]

Culture medium

LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)

[Ref.: #8044]

Culture medium growth

[Ref.: #8044]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8044]

Culture medium composition

expand / minimize

[Ref.: #33348]

Culture medium

MEDIUM 368 - for Luria bertani

[Ref.: #33348]

Culture medium growth

[Ref.: #33348]

Culture medium composition

expand / minimize

[Ref.: #116910]

Culture medium

CIP Medium 368

[Ref.: #116910]

Culture medium growth

[Ref.: #116910]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #8044]	growth	30 °C
[Ref.: #32439]	growth	07-37 °C
[Ref.: #32439]	optimum	31 °C
[Ref.: #33348]	growth	30 °C

[Ref.: #8044]	Temperature range	mesophilic
[Ref.: #32439]	Temperature range	mesophilic
[Ref.: #33348]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #32439]			optimum	7.5
[Ref.: #32439]			growth	5-9.8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #32439]	Oxygen tolerance	facultative aerobe
[Ref.: #116910]	Oxygen tolerance	obligate aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.993

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #32439]		NaCl		growth	0-2.5	%
[Ref.: #32439]		NaCl		optimum	0.85	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #32439]	adipate ChEBI	+	carbon source
[Ref.: #32439]	arabinose ChEBI	+	carbon source
[Ref.: #32439]	esculin ChEBI	+	hydrolysis
[Ref.: #32439]	gluconate ChEBI	+	carbon source
[Ref.: #32439]	glucose ChEBI	+	carbon source
[Ref.: #32439]	malate ChEBI	+	carbon source
[Ref.: #32439]	maltose ChEBI	+	carbon source
[Ref.: #32439]	mannitol ChEBI	+	carbon source
[Ref.: #32439]	mannose ChEBI	+	carbon source
[Ref.: #32439]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #116910]	nitrate ChEBI	+	reduction
[Ref.: #116910]	nitrite ChEBI	+	reduction
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #116910]		indole ChEBI	yes

	Enzyme	Enzyme activity	EC number
[Ref.: #32439]	acid phosphatase	+	3.1.3.2
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #32439]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #32439]	catalase	+	1.11.1.6
[Ref.: #116910]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #32439]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #32439]	gelatinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #116910]	oxidase	+
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #116910]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116910]	12408	-	+	+	+	-	+	+	+	+	+	+	+	-	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8044]	Sample type/isolated from	raw cow’s milk
[Ref.: #8044]	Geographic location (country and/or sea, region)	northern part
[Ref.: #8044]	Country	Israel
[Ref.: #8044]	Country ISO 3 Code	ISR
[Ref.: #8044]	Continent	Asia

[Ref.: #116910]	Sample type/isolated from	Food, Cow, raw cow's milk
[Ref.: #116910]	Country	Israel
[Ref.: #116910]	Country ISO 3 Code	ISR
[Ref.: #116910]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body Product	#Fluids	#Milk

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8044]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116910]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8044]	Chryseobacterium bovis DSM 19482 strain H9 16S ribosomal RNA gene, partial sequence	EF204446	1485	ENA	1121284 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Epilithonimonas bovis DSM 19482	GCA_900108115	scaffold	GenBank	1121284 tax ID	*
[Ref.: #66792]	Chryseobacterium bovis DSM 19482 strain DSM 19482	1121284.4	wgs	PATRIC	1121284 tax ID	*
[Ref.: #66792]	Chryseobacterium bovis DSM 19482	2595699003	draft	JGI IMG/M	1121284 tax ID	*

[Ref.: #8044]

GC-content

38.6 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Epilithonimonas bovis DSM 19482 DSM 19482 NCBI: GCA_900108115.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Epilithonimonas bovis DSM 19482 DSM 19482 NCBI: GCA_900108115.1
[Ref.: #69480]	glucose-ferment	no	92.516	no
[Ref.: #69480]	motile	no	93.707	yes
[Ref.: #69480]	flagellated	no	96.004	yes
[Ref.: #69480]	gram-positive	no	98.317	no
[Ref.: #69480]	anaerobic	no	99.128	yes
[Ref.: #69480]	aerobic	yes	64.462	yes
[Ref.: #69480]	halophile	no	95.68	yes
[Ref.: #69480]	spore-forming	no	96.296	no
[Ref.: #69480]	thermophile	no	99.797	yes
[Ref.: #69480]	glucose-util	yes	80.781	yes

Availability in culture collections External links

[Ref.: #8044]

Culture collection no.

DSM 19482, LMG 24227, CIP 110170

[Ref.: #75173]

SI-ID 343788

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	High quality permanent draft genome sequence of Chryseobacterium bovis DSM 19482(T), isolated from raw cow milk.	Laviad-Shitrit S, Goker M, Huntemann M, Clum A, Pillay M, Palaniappan K, Varghese N, Mikhailova N, Stamatis D, Reddy TBK, Daum C, Shapiro N, Markowitz V, Ivanova N, Woyke T, Klenk HP, Kyrpides NC, Halpern M	Stand Genomic Sci	10.1186/s40793-017-0242-6	2017	*
Phylogeny	Chryseobacterium bovis sp. nov., isolated from raw cow's milk.	Hantsis-Zacharov E, Senderovich Y, Halpern M	Int J Syst Evol Microbiol	10.1099/ijs.0.65500-0	2008	*

References

#8044

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19482

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#32439

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28661 (see below)

#33348 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#75173

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID343788.1 )

#116910 Collection of Institut Pasteur ; Curators of the CIP; CIP 110170

#28661

IJSEM 1024 2008 ( DOI 10.1099/ijs.0.65500-0 , PubMed 18398213 )

* These data were automatically processed and therefore are not curated

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Epilithonimonas bovis DSM 19482 DSM 19482

Epilithonimonas bovis DSM 19482 DSM 19482

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