Name: Psychroflexus gondwanensis DSM 5423
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5628

Type strain:

Species: Psychroflexus gondwanensis

Culture col. no.: DSM 5423, ACAM 44, ATCC 51278, LMG 13192, CCUG 33444, CIP 104040

Strain history: CIP <- 1994, ACAM

NCBI tax ID(s): 1189619 (strain), 251 (species)

SI-ID 7703

LMG 13192, ATCC 51278, CCUG 33444, CIP 104040, DSM 5423, NCIMB 13407, KCTC 2919

Section

Psychroflexus gondwanensis DSM 5423 is a mesophilic, Gram-negative, rod-shaped bacterium that was isolated from organic lake.

Gram-negative
rod-shaped
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Flavobacteriaceae*
	Genus	*Psychroflexus*
	Species	**Psychroflexus gondwanensis**
	Full Scientific Name (LPSN)	Psychroflexus gondwanensis corrig. (Dobson et al. 1993) Bowman et al. 1999

	Synonym	Flavobacterium gondwanense
	Synonym	Psychroflexus gondwanense

Information on morphological and physiological properties Morphology

[Ref.: #119704]	Gram stain	negative

[Ref.: #119704]	Cell shape	rod-shaped

[Ref.: #119704]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2160]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #2160]

Culture medium growth

[Ref.: #2160]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2160]

Culture medium composition

expand / minimize

[Ref.: #40940]

Culture medium

Marine agar (MA)

[Ref.: #40940]

Culture medium growth

[Ref.: #40940]

Culture medium composition

expand / minimize

[Ref.: #119704]

Culture medium

CIP Medium 13

[Ref.: #119704]

Culture medium growth

[Ref.: #119704]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #2160]		growth	28 °C
[Ref.: #40940]		growth	22 °C
[Ref.: #119704]		growth	22-25 °C
[Ref.: #119704]	no	growth	5 °C
[Ref.: #119704]	no	growth	15 °C
[Ref.: #119704]	no	growth	37 °C
[Ref.: #119704]	no	growth	41 °C

[Ref.: #2160]	Temperature range	mesophilic
[Ref.: #40940]	Temperature range	psychrophilic
[Ref.: #119704]	Temperature range	psychrophilic
[Ref.: #119704]	Temperature range	mesophilic
[Ref.: #119704]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #119704]	citrate ChEBI	-	carbon source
[Ref.: #119704]	esculin ChEBI	+	hydrolysis
[Ref.: #119704]	nitrate ChEBI	-	reduction
[Ref.: #119704]	nitrite ChEBI	-	reduction

	Enzyme	Enzyme activity	EC number
[Ref.: #119704]	amylase	-
[Ref.: #119704]	caseinase	-	3.4.21.50
[Ref.: #119704]	catalase	+	1.11.1.6
[Ref.: #119704]	DNase	+
[Ref.: #119704]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #119704]	gelatinase	+
[Ref.: #119704]	lecithinase	-
[Ref.: #119704]	lipase	-
[Ref.: #119704]	oxidase	+

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #51523]

Fatty acid	Percentage	ECL
C15:0 ANTEISO	32.8	14.711
C16:0 iso 3OH	17.6	17.145
C15:1 ANTEISO A	16.7	14.526
C14:0 ISO	6.6	13.618
C16:0 iso	5.9	15.626
Unidentified	5.9	18.272
C15:0 2OH	4	16.217
C12:0 3OH	3.4	13.455
C15:0 ISO	1.5	14.621
C15:0	1.2	15
Unidentified	1.1	15.59
C14:0 ISO 3OH	0.8	15.117
C17:0 iso 3OH	0.8	18.161
C13:0 3OH/C15:1 i I/H	0.8	14.469
C13:0 ANTEISO	0.5	12.701
C15:0 3OH	0.3	16.504

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2160]	Sample type/isolated from	organic lake
[Ref.: #2160]	Continent	Australia and Oceania

[Ref.: #119704]	Sample type/isolated from	Environment, Organic lake, depth 5m
[Ref.: #119704]	Country	Antarctica
[Ref.: #119704]	Country ISO 3 Code	ATA
[Ref.: #119704]	Continent	Antarctica
[Ref.: #119704]	Isolation date	1987

[Ref.: #51523]	Sampling date	1987
[Ref.: #51523]	Geographic location (country and/or sea, region)	Vestfold Hills
[Ref.: #51523]	Country	Antarctica
[Ref.: #51523]	Country ISO 3 Code	ATA
[Ref.: #51523]	Continent	Australia and Oceania
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Lake (large)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JX986967 (>99% sequence identity) for Psychroflexus gondwanensis from Microbeatlas

Aquatic Samples	184
Soil Samples	12
Animal Samples	7
Plant Samples
Total Samples	203

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2160]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119704]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #2160]	Psychroflexus gondwanensis strain LMG 13192 16S ribosomal RNA gene, partial sequence	JX986967	1475	ENA	251 tax ID
[Ref.: #20218]	Psychroflexus gondwanensis partial 16S rRNA gene, type strain DSM 5423T	FR849911	1476	ENA	1189619 tax ID	*
[Ref.: #20218]	Flavobacterium gondwanense 16S ribosomal RNA gene	M92278	1476	ENA	251 tax ID	*

[Ref.: #2160]

GC-content

35.8 mol%

sequence analysis

Availability in culture collections External links

[Ref.: #2160]

Culture collection no.

DSM 5423, ACAM 44, ATCC 51278, LMG 13192, CCUG 33444, CIP 104040

[Ref.: #75111]

SI-ID 7703

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Psychroflexus halocasei sp. nov., isolated from a microbial consortium on a cheese.	Seiler H, Bleicher A, Busse HJ, Hufner J, Scherer S	Int J Syst Evol Microbiol	10.1099/ijs.0.034801-0	2011	*
Phylogeny	Direct sequencing of the polymerase chain reaction-amplified 16S rRNA gene of Flavobacterium gondwanense sp. nov. and Flavobacterium salegens sp. nov., two new species from a hypersaline Antarctic lake.	Dobson SJ, Colwell RR, McMeekin TA, Franzmann PD	Int J Syst Bacteriol	10.1099/00207713-43-1-77	1993	*

References

#2160

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 5423

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40940 ; Curators of the CIP;
#51523 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 33444

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#75111

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID7703.1 )

#119704 Collection of Institut Pasteur ; Curators of the CIP; CIP 104040
* These data were automatically processed and therefore are not curated

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