Name: Acidovorax delafieldii 133
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2885

Type strain:

Species: Acidovorax delafieldii

Strain Designation: 133, FD-6

Culture col. no.: CCUG 1779, LMG 5943, DSM 64, ATCC 17505, DSM 50263, DSM 50403, CIP 103303, NCIMB 11476

Strain history: CIP <- 1989, DSM <- Inst. Mikrobiol. Univ., Göttinger, Germany <- R.Y. Stanier, Inst. Pasteur, Paris, France: strain 133, Pseudomonas delafieldii <- F. Delafield: strain FD-6

NCBI tax ID(s): 47920 (species)

SI-ID 92080

LMG 5943, ATCC 17505, CCUG 1779, DSM 50263, DSM 50403, DSM 64, LMG 6832, NCIB 11476, CIP 103303, NCIMB 11476, CCM 3589, LMD 90.17, CECT 4105, CCRC 13888, CFBP 2442, NCCB 90017, BCRC 13888, CFBP 3609, CGMCC 1.2784

Other strains from Acidovorax delafieldii

A. delafieldii FD-7, DSM 4762, ATCC 17506, CCUG ...
A. delafieldii CIP 103454, CCUG 16516, LMG 8905
A. delafieldii CIP 103455, CCUG 18251, LMG 7186
A. delafieldii CIP 106437, CCUG 11056, LMG 7166
A. delafieldii CIP 106438, CCUG 11062, LMG 7168
A. delafieldii CCUG 12929, LMG 7167
A. delafieldii CCUG 14478, ATCC 17508, LMG 5944
A. delafieldii CCUG 16566, LMG 8906
A. delafieldii CCUG 20074, LMG 7679
A. delafieldii CCUG 21358, LMG 8916
A. delafieldii CCUG 24594, LMG 9090
A. delafieldii IMI 359867

Section

Acidovorax delafieldii 133 is an obligate aerobe, mesophilic, Gram-negative human pathogen that was isolated from soil, enrichment with poly-ß-hydroxybutyrate.

Gram-negative
motile
rod-shaped
human pathogen
mesophilic
obligate aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Comamonadaceae*
	Genus	*Acidovorax*
	Species	**Acidovorax delafieldii**
	Full Scientific Name (LPSN)	Acidovorax delafieldii (Davis 1970) Willems et al. 1990

Synonym

Pseudomonas delafieldii

Information on morphological and physiological properties Morphology

[Ref.: #118877]	Gram stain	negative

[Ref.: #118877]	Cell shape	rod-shaped

[Ref.: #118877]	Motility	yes

[Ref.: #118877]	Pigment production	no
[Ref.: #118877]	Pigment name	Pyocyanin

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2047]

Culture medium

REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)

[Ref.: #2047]

Culture medium growth

[Ref.: #2047]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2047]

Culture medium composition

expand / minimize

[Ref.: #2047]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #2047]

Culture medium growth

[Ref.: #2047]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2047]

Culture medium composition

expand / minimize

[Ref.: #40594]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #40594]

Culture medium growth

[Ref.: #40594]

Culture medium composition

expand / minimize

[Ref.: #118877]

Culture medium

CIP Medium 72

[Ref.: #118877]

Culture medium growth

[Ref.: #118877]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #2047]		growth	30 °C
[Ref.: #40594]		growth	25 °C
[Ref.: #118877]		growth	5-37 °C
[Ref.: #118877]	no	growth	41 °C

[Ref.: #2047]	Temperature range	mesophilic
[Ref.: #40594]	Temperature range	mesophilic
[Ref.: #118877]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #118877]

Oxygen tolerance

obligate aerobe

	Salt	Tested relation		Salt conc.
[Ref.: #118877]	NaCl		growth	0-2	%
[Ref.: #118877]	NaCl	no	growth	4	%
[Ref.: #118877]	NaCl	no	growth	6	%
[Ref.: #118877]	NaCl	no	growth	8	%
[Ref.: #118877]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #118877]	citrate ChEBI	-	carbon source
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #118877]	esculin ChEBI	-	hydrolysis
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #118877]	nitrate ChEBI	+	reduction
[Ref.: #118877]	nitrate ChEBI	-	respiration
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #118877]	nitrite ChEBI	-	reduction
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #118877]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #118877]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #118877]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #118877]	amylase	-
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #118877]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API 20NE
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #118877]	caseinase	+	3.4.21.50
[Ref.: #118877]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #118877]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #118877]	gelatinase	-
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #118877]	lecithinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #118877]	lipase	+
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #118877]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #118877]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #118877]	oxidase	+
[Ref.: #118877]	protease	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #118877]	tryptophan deaminase	-
[Ref.: #118877]	tween esterase	+
[Ref.: #118877]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #44419]

Fatty acid	Percentage	ECL
C16:1 ω7c	47	15.819
C16:0	21.8	16
C18:1 ω7c /12t/9t	15.4	17.824
C14:0	4.5	14
C12:0	3.9	12
C10:0 3OH	3.8	11.423
C18:2 ω6,9c/C18:0 ANTE	2.8	17.724
C8:0 3OH	0.9	9.385

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #2047]	24894	+	-	-	-	+	-	-	-	-	+	+	+	-	-	+	-	-	+	-	-	+
[Ref.: #44419]	7331	+	-	-	-	-	-	-	-	+	+	+	+	-	-	+	-	-	+	-	-	+
[Ref.: #44419]	7332	+	-	+	-	-	-	-	-	+	+	+	+	-	-	+	-	-	+	-	-	+
[Ref.: #2047]	23343	+	-	-	-	-	-	-	-	+	+	+	+	-	-	+	-	-	+	-	-	+

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #118877]	387	not determinedn.d.	+	+	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	+	-	-	-	-	+	-	+	-	-	+	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	+	-	-	-	+	-	+	+	+	+	-	-	-	-	-	-	-	+	-	+	-	-	-	+	+	-	-	-	-	-	+	-	+	+	+	+	-	+	+	-	-	+	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #44419]	3685	-	+	+	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #118877]	7487	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2047]	Sample type/isolated from	soil, enrichment with poly-ß-hydroxybutyrate

[Ref.: #44419]	Sample type/isolated from	Soil,enrichment with PHB as sole carbon source

[Ref.: #118877]	Sample type/isolated from	Environment, Soil enriched with poly-b-hydroxybuty
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AF078764 (>99% sequence identity) for Acidovorax from Microbeatlas

Aquatic Samples	155
Soil Samples	75
Animal Samples	38
Plant Samples	904
Total Samples	1172

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2047]	Pathogenicity (human)	yes, in single cases
[Ref.: #2047]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118877]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Pseudomonas putida strain 133 16S ribosomal RNA gene, partial sequence	JQ795146	403	ENA	303 tax ID	*
[Ref.: #20218]	Acidovorax delafieldii 16S ribosomal RNA gene, partial sequence	AF078764	1515	ENA	47920 tax ID	*
[Ref.: #20218]	Acidovorax delafieldii 16S rRNA gene, strain DSM 50263	AJ420323	1483	ENA	47920 tax ID	*
[Ref.: #20218]	Acidovorax delafieldii strain LMG 5943 16S ribosomal RNA gene, partial sequence	EU024132	1318	ENA	47920 tax ID	*

[Ref.: #2047]

GC-content

64.8 mol%

Availability in culture collections External links

[Ref.: #2047]

Culture collection no.

CCUG 1779, LMG 5943, DSM 64, ATCC 17505, DSM 50263, DSM 50403, CIP 103303, NCIMB 11476

[Ref.: #72408]

SI-ID 92080

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Acidovorax kalamii sp. nov., isolated from a water sample of the river Ganges.	Pal D, Kaur N, Sudan SK, Bisht B, Krishnamurthi S, Mayilraj S	Int J Syst Evol Microbiol	10.1099/ijsem.0.002736	2018	*
Metabolism	Regulation of pyrimidine biosynthesis in Acidovorax delafieldii.	West TP	J Basic Microbiol	10.1002/jobm.200410506	2005	*
Phylogeny	Acidovorax, a new genus for Pseudomonas facilis, Pseudomonas delafieldii, E. Falsen (EF) group 13, EF group 16, and several clinical isolates, with the species Acidovorax facilis comb. nov., Acidovorax delafieldii comb. nov., and Acidovorax temperans sp. nov.	Willems A, Falsen E, Pot B, Jantzen E, Hoste B, Vandamme P, Gillis M, Kersters K, De Ley J	Int J Syst Bacteriol	10.1099/00207713-40-4-384	1990	*

References

#2047

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 64

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40594 ; Curators of the CIP;
#44419 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 1779

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#72408

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID92080.1 )

#118877 Collection of Institut Pasteur ; Curators of the CIP; CIP 103303
* These data were automatically processed and therefore are not curated

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