Name: Aeromonas salmonicida subsp. smithia 138
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 273

Type strain:

Species: Aeromonas salmonicida subsp. smithia

Strain Designation: 138, AS20/1/1

Culture col. no.: DSM 21293, ATCC 49393, CCM 4103, CECT 5179, CIP 104757, NCIMB 13210

Strain history: CIP <- 1996, NCIB <- CCM <- Austin: strain 138 <- T. Owen: strain AS20/1/1

NCBI tax ID(s): 80745 (subspecies)

SI-ID 92494

LMG 16264, CCM 4103, LMG 10893, ATCC 49393, NCIMB 13210, LMG 20223, CIP 104757, CECT 5179, BCRC 15864, CCRC 15864, DSM 21293

Section

Aeromonas salmonicida subsp. smithia 138 is a facultative anaerobe, psychrophilic, Gram-negative animal pathogen that was isolated from Ulcer .

Gram-negative
rod-shaped
animal pathogen
facultative anaerobe
psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Aeromonadales*
	Family	*Aeromonadaceae*
	Genus	*Aeromonas*
	Species	**Aeromonas salmonicida subsp. smithia**
	Full Scientific Name (LPSN)	Aeromonas salmonicida subsp. smithia Austin et al. 1989

Information on morphological and physiological properties Morphology

[Ref.: #66548]	Gram stain	negative
[Ref.: #122394]	Gram stain	negative

[Ref.: #66548]	Cell length	1-2 µm

[Ref.: #66548]	Cell shape	rod-shaped
[Ref.: #122394]	Cell shape	rod-shaped

[Ref.: #66548]	Motility	no
[Ref.: #122394]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15607]

Culture medium

BHI MEDIUM (DSMZ Medium 215)

[Ref.: #15607]

Culture medium growth

[Ref.: #15607]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15607]

Culture medium composition

expand / minimize

[Ref.: #41291]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #41291]

Culture medium growth

[Ref.: #41291]

Culture medium composition

expand / minimize

[Ref.: #66548]

Culture medium

peptone broth

[Ref.: #66548]

Culture medium growth

[Ref.: #66548]

Culture medium

tryptic soy agar (TSA)

[Ref.: #66548]

Culture medium growth

[Ref.: #122394]

Culture medium

CIP Medium 72

[Ref.: #122394]

Culture medium growth

[Ref.: #122394]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #122394]

Culture medium

CIP Medium 3

[Ref.: #122394]

Culture medium growth

[Ref.: #122394]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #66548]

Culture medium

MacConkey agar

[Ref.: #66548]

Culture medium growth

	Kind of temperature		Temperature
[Ref.: #15607]		growth	20 °C
[Ref.: #41291]		growth	22 °C
[Ref.: #66548]	no	growth	30 °C
[Ref.: #66548]		growth	4-25 °C
[Ref.: #122394]		growth	5-22 °C
[Ref.: #122394]	no	growth	30 °C
[Ref.: #122394]	no	growth	37 °C
[Ref.: #122394]	no	growth	41 °C

[Ref.: #15607]	Temperature range	psychrophilic
[Ref.: #41291]	Temperature range	psychrophilic
[Ref.: #66548]	Temperature range	mesophilic
[Ref.: #122394]	Temperature range	psychrophilic
[Ref.: #122394]	Temperature range	mesophilic
[Ref.: #122394]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #66548]		no	growth	4
[Ref.: #66548]		no	growth	5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #122394]

Oxygen tolerance

facultative anaerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66548]		NaCl		growth	0-2	%(w/v)
[Ref.: #66548]		NaCl	no	growth	3	%(w/v)

[Ref.: #66548]	Observation	Cultures on TSA dissociate into rough, smooth, and G-phase colonies, which do not readily produce diffusible brown pigment. In peptone broth cultures are uniformly turbid.
[Ref.: #66548]	Observation	With cluster 11, mortalities commenced within 24 h and all the infected stock were dead within 72 h, even after challenge with a minimum of 6.2 x 10-4 cellslfish. External haemorrhages were evident in the vent and on the fins. With dissection, an accumulation of ascitic fluid in the peritoneal cavity, extensive muscle haemorrhaging and liquefaction and gastro-enteritis were apparent. With the addition of tail rot, similar damage was recorded in fish injected with isolates from cluster 14 (includ

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	arginine ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #66548]	beta-D-fructofuranose ChEBI	-	carbon source
[Ref.: #66548]	cellobiose ChEBI	-	builds acid from
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #66548]	chitin ChEBI	-	degradation
[Ref.: #122394]	citrate ChEBI	-	carbon source
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68369]	D-glucose ChEBI	+	fermentation	* from API 20NE, API 20E
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	D-mannitol ChEBI	-	fermentation	* from API 20E
[Ref.: #66548]	D-mannose ChEBI	-	carbon source
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #66548]	D-ribose ChEBI	-	carbon source
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #66548]	D-xylose ChEBI	-	carbon source
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #66548]	disodium fumarate ChEBI	-	carbon source
[Ref.: #66548]	disodium malate ChEBI	-	carbon source
[Ref.: #66548]	dl-alanine ChEBI	-	carbon source
[Ref.: #66548]	dna ChEBI	+	degradation
[Ref.: #66548]	elastin ChEBI	-	degradation
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #66548]	esculin ChEBI	-	degradation
[Ref.: #122394]	esculin ChEBI	-	hydrolysis
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #66548]	galactose ChEBI	-	builds acid from
[Ref.: #66548]	gelatin ChEBI	+	degradation
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE, API 20E
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #66548]	glucose ChEBI	+	builds acid from
[Ref.: #66548]	glucose ChEBI	-	fermentation
[Ref.: #122394]	glucose ChEBI	+	fermentation
[Ref.: #122394]	glucose ChEBI	+	degradation
[Ref.: #66548]	glycerol ChEBI	-	builds acid from
[Ref.: #66548]	guanine ChEBI	-	degradation
[Ref.: #66548]	hypoxanthine ChEBI	-	degradation
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #66548]	L-arabinose ChEBI	-	carbon source
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	L-arabinose ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #66548]	L-arginine ChEBI	-	carbon source
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #66548]	L-histidine ChEBI	-	carbon source
[Ref.: #66548]	L-leucine ChEBI	-	carbon source
[Ref.: #66548]	L-proline ChEBI	-	carbon source
[Ref.: #66548]	L-rhamnose ChEBI	-	carbon source
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	L-rhamnose ChEBI	-	fermentation	* from API 20E
[Ref.: #66548]	L-serine ChEBI	-	carbon source
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #66548]	L-threonine ChEBI	-	carbon source
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #66548]	lactose ChEBI	-	builds acid from
[Ref.: #122394]	lactose ChEBI	-	fermentation
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #66548]	lecithin ChEBI	-	degradation
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #122394]	malonate ChEBI	-	assimilation
[Ref.: #66548]	maltose ChEBI	-	builds acid from
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #66548]	mannitol ChEBI	-	builds acid from
[Ref.: #122394]	mannitol ChEBI	-	fermentation
[Ref.: #66548]	melezitose ChEBI	-	carbon source
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	melibiose ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #66548]	myo-inositol ChEBI	-	builds acid from
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #122394]	nitrate ChEBI	-	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #122394]	nitrite ChEBI	-	reduction
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #66548]	raffinose ChEBI	-	builds acid from
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #66548]	rna ChEBI	+	degradation
[Ref.: #66548]	salicin ChEBI	-	carbon source
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #66548]	sodium citrate ChEBI	-	carbon source
[Ref.: #66548]	sodium glutamate ChEBI	-	carbon source
[Ref.: #66548]	sodium malonate ChEBI	-	carbon source
[Ref.: #66548]	sodium pyruvate ChEBI	-	carbon source
[Ref.: #66548]	sodium succinate ChEBI	-	carbon source
[Ref.: #122394]	sodium thiosulfate ChEBI	-	builds gas from
[Ref.: #66548]	sorbitol ChEBI	-	builds acid from
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #66548]	starch ChEBI	+	degradation
[Ref.: #66548]	sucrose ChEBI	+	builds acid from
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68368]	sucrose ChEBI	+	fermentation	* from API 20E
[Ref.: #66548]	trehalose ChEBI	-	builds acid from
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE, API 20E
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #66548]	tween 20 ChEBI	-	degradation
[Ref.: #66548]	tween 40 ChEBI	-	degradation
[Ref.: #66548]	tween 60 ChEBI	-	degradation
[Ref.: #66548]	tween 80 ChEBI	-	degradation
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE, API 20E
[Ref.: #66548]	xanthine ChEBI	-	degradation
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #66548]	acetoin ChEBI	no
[Ref.: #66548]	hydrogen sulfide ChEBI	yes
[Ref.: #66548]	indole ChEBI	no
[Ref.: #122394]	indole ChEBI	no
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68369]	indole ChEBI	no	* from API 20NE, API 20E

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #66548]	acetoin ChEBI	-
[Ref.: #122394]	acetoin ChEBI	-
[Ref.: #66548]	glucose ChEBI		-
[Ref.: #122394]	glucose ChEBI		-
[Ref.: #68368]	acetoin ChEBI	-			* from API 20E
[Ref.: #68369]	indole ChEBI			-	* from API 20NE, API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #122394]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #66548]	arginine decarboxylase	-	4.1.1.19
[Ref.: #68368]	arginine dihydrolase	+	3.5.3.6	* from API 20E
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #66548]	beta-galactosidase	+	3.2.1.23
[Ref.: #122394]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API 20E
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API 20NE
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #66548]	catalase	+	1.11.1.6
[Ref.: #122394]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #66548]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68368]	cytochrome oxidase	+	1.9.3.1	* from API 20E
[Ref.: #68369]	cytochrome oxidase	-	1.9.3.1	* from API 20NE
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #122394]	gelatinase	+
[Ref.: #68369]	gelatinase	+		* from API 20NE, API 20E
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #66548]	lysine decarboxylase	-	4.1.1.18
[Ref.: #122394]	lysine decarboxylase	+	4.1.1.18
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #66548]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #122394]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #122394]	oxidase	+
[Ref.: #122394]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #66548]	phenylalanine deaminase	-	4.3.1.5
[Ref.: #66548]	phosphatase	+
[Ref.: #66548]	phosphoamidase	+	3.9.1.1
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #122394]	tryptophan deaminase	-
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #122394]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE, API 20E
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.