Name: Clostridium beijerinckii E. McCoy A-67
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2548

Type strain:

Species: Clostridium beijerinckii

Strain Designation: E. McCoy A-67, L.S. McClung 1671, 10026

Culture col. no.: DSM 791, ATCC 25752, NCIMB 9362, CCUG 56442, CIP 104308, NCTC 13035, LMG 5716, JCM 1390, VPI 5481, ATCC 17778, BCRC 14488, NBRC 109359, VTT E-91425

Strain history: CIP <- 1994, J.P. Carlier, Inst. Pasteur, Paris, France: strain 10026 <- ATCC <- L.D.S. Smith: strain VPI 5481

NCBI tax ID(s): 1520 (species)

SI-ID 92210

LMG 5716, ATCC 17778, ATCC 25752, DSM 791, NCIB 9362, CIP 104308, JCM 1390, CCRC 14488, NCIMB 9362, NCTC 13035, BCRC 14488, VTT E-91425, CCUG 56442

Other strains from Clostridium beijerinckii

Section

Clostridium beijerinckii E. McCoy A-67 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from Soil.

motile
spore-forming
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Clostridium*
	Species	**Clostridium beijerinckii**
	Full Scientific Name (LPSN)	Clostridium beijerinckii Donker 1926 (Approved Lists 1980)

Synonym

Clostridium diolis

Information on morphological and physiological properties Morphology

[Ref.: #116776]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #116776]	Cell shape	rod-shaped

[Ref.: #116776]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %93.956

Information on culture and growth conditions Culture and growth conditions

[Ref.: #448]

Culture medium

CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)

[Ref.: #448]

Culture medium growth

[Ref.: #448]

Culture medium link

Medium recipe at MediaDive

[Ref.: #448]

Culture medium composition

expand / minimize

[Ref.: #39664]

Culture medium

MEDIUM 20 - for Anaerobic bacteria

[Ref.: #39664]

Culture medium growth

[Ref.: #39664]

Culture medium composition

expand / minimize

[Ref.: #116776]

Culture medium

CIP Medium 20

[Ref.: #116776]

Culture medium growth

[Ref.: #116776]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #448]	growth	37 °C
[Ref.: #39664]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #448]	Temperature range	mesophilic
[Ref.: #39664]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #448]	Oxygen tolerance	anaerobe
[Ref.: #116776]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.156

[Ref.: #116776]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %99
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #116776]		nitrate ChEBI	-	reduction
[Ref.: #116776]		nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #67770]		butanol	yes
[Ref.: #116776]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #116776]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #116776]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #116776]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116776]	7885	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Soil
* marker position based on {}

[Ref.: #69479]

Global distribution of 16S sequence AB643462 (>99% sequence identity) for Clostridium from Microbeatlas

Aquatic Samples	1977
Soil Samples	1985
Animal Samples	5931
Plant Samples	442
Total Samples	10335

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #448]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116776]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #448]	C.beijerinckii (DSM791) rrn gene for 16S rRNA	X68179	1510	ENA	1520 tax ID
[Ref.: #67770]	Clostridium beijerinckii gene for 16S ribosomal RNA, partial sequence, strain: JCM 1390	AB643462	1473	ENA	1520 tax ID
[Ref.: #20218]	Clostridium beijerinckii strain DSM 791 16S ribosomal RNA gene, partial sequence	HM245931	837	ENA	1520 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Clostridium beijerinckii DSM 791	GCA_002006445	contig	GenBank	1520 tax ID
[Ref.: #66792]	Clostridium beijerinckii NCTC13035	GCA_900447025	contig	GenBank	1520 tax ID	*
[Ref.: #66792]	Clostridium beijerinckii NBRC 109359	GCA_007992515	contig	GenBank	1520 tax ID	*
[Ref.: #66792]	Clostridium beijerinckii strain DSM 791	1520.584	complete	PATRIC	1520 tax ID	*
[Ref.: #66792]	Clostridium beijerinckii strain DSM 791	1520.98	wgs	PATRIC	1520 tax ID	*
[Ref.: #66792]	Clostridium beijerinckii strain NBRC 109359	1520.326	wgs	PATRIC	1520 tax ID	*

[Ref.: #448]	GC-content	28.0 mol%
[Ref.: #67770]	GC-content	28 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	31.2 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Clostridium beijerinckii DSM 791 NCBI: GCA_018223745.1
[Ref.: #69481]	spore-forming	yes	99	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Clostridium beijerinckii DSM 791 NCBI: GCA_018223745.1
[Ref.: #69480]	flagellated	yes	68.655	no
[Ref.: #69480]	gram-positive	yes	65.22	no
[Ref.: #69480]	anaerobic	yes	98.175	yes
[Ref.: #69480]	aerobic	no	97.087	yes
[Ref.: #69480]	halophile	no	98.41	no
[Ref.: #69480]	spore-forming	yes	95.39	no
[Ref.: #69480]	thermophile	no	99.13	no
[Ref.: #69480]	glucose-util	yes	89.032	no
[Ref.: #69480]	motile	yes	85.104	no
[Ref.: #69480]	glucose-ferment	yes	64.547	no

Availability in culture collections External links

[Ref.: #448]

Culture collection no.

DSM 791, ATCC 25752, NCIMB 9362, CCUG 56442, CIP 104308, NCTC 13035, LMG 5716, JCM 1390, VPI 5481, ATCC 17778, BCRC 14488, NBRC 109359, VTT E-91425

[Ref.: #72081]

SI-ID 92210

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Biotechnology	Augmented biohydrogen production from rice mill wastewater through nano-metal oxides assisted dark fermentation.	Rambabu K, Bharath G, Thanigaivelan A, Das DB, Show PL, Banat F	Bioresour Technol	10.1016/j.biortech.2020.124243	2020	*
Phylogeny	Reclassification of Clostridium diolis Biebl and Sproer 2003 as a later heterotypic synonym of Clostridium beijerinckii Donker 1926 (Approved Lists 1980) emend. Keis et al. 2001.	Kobayashi H, Tanizawa Y, Sakamoto M, Nakamura Y, Ohkuma M, Tohno M	Int J Syst Evol Microbiol	10.1099/ijsem.0.004059	2020	*
Metabolism	Production of 1,3-propanediol by Clostridium beijerinckii DSM 791 from crude glycerol and corn steep liquor: Process optimization and metabolic engineering.	Wischral D, Zhang J, Cheng C, Lin M, De Souza LMG, Pessoa FLP, Pereira N Jr, Yang ST	Bioresour Technol	10.1016/j.biortech.2016.04.020	2016	*
Phylogeny	Clostridium chromiireducens sp. nov., isolated from Cr(VI)-contaminated soil.	Inglett KS, Bae HS, Aldrich HC, Hatfield K, Ogram AV	Int J Syst Evol Microbiol	10.1099/ijs.0.024554-0	2010	*
Transcriptome	Genetic features of circular bacteriocins produced by Gram-positive bacteria.	Maqueda M, Sanchez-Hidalgo M, Fernandez M, Montalban-Lopez M, Valdivia E, Martinez-Bueno M	FEMS Microbiol Rev	10.1111/j.1574-6976.2007.00087.x	2007	*
Metabolism	The circular bacteriocins gassericin A and circularin A.	Kawai Y, Kemperman R, Kok J, Saito T	Curr Protein Pept Sci	10.2174/1389203043379549	2004	*
Metabolism	Functional analysis of the gene cluster involved in production of the bacteriocin circularin A by Clostridium beijerinckii ATCC 25752.	Kemperman R, Jonker M, Nauta A, Kuipers OP, Kok J	Appl Environ Microbiol	10.1128/AEM.69.10.5839-5848.2003	2003	*
Metabolism	Identification and characterization of two novel clostridial bacteriocins, circularin A and closticin 574.	Kemperman R, Kuipers A, Karsens H, Nauta A, Kuipers O, Kok J	Appl Environ Microbiol	10.1128/AEM.69.3.1589-1597.2003	2003	*
Phylogeny	Taxonomy of the glycerol fermenting clostridia and description of Clostridium diolis sp. nov.	Biebl H, Sproer C	Syst Appl Microbiol	10.1078/07232020260517616	2002	*
Phylogeny	Cultures of "Clostridium acetobutylicum" from various collections comprise Clostridium acetobutylicum, Clostridium beijerinckii, and two other distinct types based on DNA-DNA reassociation.	Johnson JL, Toth J, Santiwatanakul S, Chen JS	Int J Syst Bacteriol	10.1099/00207713-47-2-420	1997	*

References

#448	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 791

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#39664 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#72081

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID92210.1 )

#116776 Collection of Institut Pasteur ; Curators of the CIP; CIP 104308
* These data were automatically processed and therefore are not curated

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Clostridium beijerinckii DSM 791

Clostridium beijerinckii DSM 791

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