Name: Burkholderia metallica AU0553
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1986

Type strain:

Species: Burkholderia metallica

Strain Designation: AU0553

Culture col. no.: DSM 23519, CCUG 54567, LMG 24068

Strain history: <- CCUG <- E. Vanlaere, LMG <- J. J. LiPuma; AU0553

NCBI tax ID(s): 488729 (species)

SI-ID 95222

LMG 24068, CCUG 54567, DSM 23519

Other strains from Burkholderia metallica

B. metallica CCUG 54568, LMG 24274
B. metallica CCUG 54569, LMG 24275

Section

Burkholderia metallica AU0553 is an aerobe, mesophilic, Gram-negative bacterium that has a yellow pigmentation and was isolated from human sputum from cystic fibrosis patient.

Gram-negative
pigmented
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Burkholderia*
	Species	**Burkholderia metallica**
	Full Scientific Name (LPSN)	Burkholderia metallica Vanlaere et al. 2008

Information on morphological and physiological properties Morphology

[Ref.: #23365]	Gram stain	negative

[Ref.: #23365]	Cell shape	rod-shaped

[Ref.: #17255]	Type of hemolysis	gamma

[Ref.: #23365]	Colony color	metallic shine

[Ref.: #23365]	Pigment production	yes
[Ref.: #23365]	Pigmentation color	yellow

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17255]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #17255]

Culture medium growth

[Ref.: #17255]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17255]

Culture medium composition

expand / minimize

[Ref.: #17255]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #17255]

Culture medium growth

[Ref.: #17255]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17255]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #17255]	growth	30 °C
[Ref.: #23365]	growth	30-42 °C
[Ref.: #60241]	growth	37 °C

[Ref.: #17255]	Temperature range	mesophilic
[Ref.: #60241]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23365]	Oxygen tolerance	aerobe
[Ref.: #60241]	Oxygen tolerance	aerobe

[Ref.: #23365]

Ability of spore formation

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23365]	adipate ChEBI	+	assimilation
[Ref.: #68369]	adipate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #23365]	citrate ChEBI	+	growth
[Ref.: #23365]	D-gluconate ChEBI	+	growth
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #23365]	D-mannitol ChEBI	+	assimilation
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #23365]	D-mannose ChEBI	+	growth
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #23365]	decanoate ChEBI	+	assimilation
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #23365]	esculin ChEBI	+	hydrolysis
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #23365]	glucose ChEBI	+	builds acid from
[Ref.: #23365]	glucose ChEBI	+	growth
[Ref.: #23365]	L-arabinose ChEBI	+	assimilation
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #23365]	L-malate ChEBI	+	growth
[Ref.: #23365]	lactose ChEBI	+	builds acid from
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #23365]	maltose ChEBI	-	assimilation
[Ref.: #23365]	maltose ChEBI	+	builds acid from
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #23365]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #23365]	nitrate ChEBI	-	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #23365]	phenylacetate ChEBI	+	assimilation
[Ref.: #23365]	ribitol ChEBI	+	builds acid from
[Ref.: #23365]	sucrose ChEBI	+	builds acid from
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #23365]	xylose ChEBI	+	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #23365]	arginine dihydrolase	-	3.5.3.6
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #23365]	beta-galactosidase	+	3.2.1.23
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #17255]	catalase	+	1.11.1.6
[Ref.: #23365]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #17255]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #23365]	gelatinase	+
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #23365]	lysine decarboxylase	+	4.1.1.18
[Ref.: #23365]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #23365]	tryptophanase	-	4.1.99.1
[Ref.: #23365]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #17255]	23866	-	-	+/-	-	-	+	+	+	+	+	+	+	+	-	+	+	+	+	+	+	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17255]	Sample type/isolated from	human sputum from cystic fibrosis patient
[Ref.: #17255]	Country	USA
[Ref.: #17255]	Country ISO 3 Code	USA
[Ref.: #17255]	Continent	North America

[Ref.: #60241]	Sample type/isolated from	Human sputum,cystic fibrosis
[Ref.: #60241]	Sampling date	2001-09-07
[Ref.: #60241]	Country	USA
[Ref.: #60241]	Country ISO 3 Code	USA
[Ref.: #60241]	Continent	North America

[Ref.: #23365]	Sampling date	1998
* marker position based on {}

Isolation sources categories

#Infection	#Disease	#Cystic fibrosis
#Infection	#Patient	-
#Host	#Human	-
#Host Body Product	#Fluids	#Sputum

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AM747632 (>99% sequence identity) for Burkholderia from Microbeatlas

Aquatic Samples	564
Soil Samples	1055
Animal Samples	1930
Plant Samples	1505
Total Samples	5054

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17255]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #17255]	Burkholderia metallica partial 16S rRNA gene, type strain R-16017T	AM747632	1505	ENA	488729 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Burkholderia metallica LMG 24068	GCA_902832845	scaffold	GenBank	488729 tax ID	*
[Ref.: #66792]	Burkholderia metallica LMG 24068	GCA_902499065	contig	GenBank	488729 tax ID	*
[Ref.: #66792]	Burkholderia metallica strain LMG 24068	488729.11	wgs	PATRIC	488729 tax ID	*
[Ref.: #66792]	Burkholderia metallica strain LMG 24068	488729.16	wgs	PATRIC	488729 tax ID	*

[Ref.: #23365]

GC-content

67.0 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Burkholderia metallica LMG 24068 NCBI: GCA_902832845.1
[Ref.: #69480]	flagellated	no	81.543	no
[Ref.: #69480]	gram-positive	no	98.031	no
[Ref.: #69480]	anaerobic	no	98.478	yes
[Ref.: #69480]	aerobic	yes	89.545	yes
[Ref.: #69480]	halophile	no	92.513	no
[Ref.: #69480]	spore-forming	no	92.999	yes
[Ref.: #69480]	thermophile	no	99.531	yes
[Ref.: #69480]	glucose-util	yes	91.604	yes
[Ref.: #69480]	motile	yes	89.448	no
[Ref.: #69480]	glucose-ferment	no	87.1	no

Availability in culture collections External links

[Ref.: #17255]

Culture collection no.

DSM 23519, CCUG 54567, LMG 24068

[Ref.: #71633]

SI-ID 95222

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex.	Vanlaere E, Lipuma JJ, Baldwin A, Henry D, De Brandt E, Mahenthiralingam E, Speert D, Dowson C, Vandamme P	Int J Syst Evol Microbiol	10.1099/ijs.0.65634-0	2008	*

References

#17255

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23519

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23365

Elke Vanlaere, John J. LiPuma, Adam Baldwin, Deborah Henry, Evie De Brandt, Eshwar Mahenthiralingam, David Speert, Chris Dowson, Peter Vandamme: Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex. IJSEM 58: 1580 - 1590 2008 ( DOI 10.1099/ijs.0.65634-0 , PubMed 18599699 )

#60241 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 54567

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#71633

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID95222.1 )

* These data were automatically processed and therefore are not curated

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Burkholderia metallica LMG 24068

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