Name: Methanoregula boonei 6A8
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17856

Type strain:

Species: Methanoregula boonei

Strain Designation: 6A8

Culture col. no.: DSM 21154, JCM 14090

Strain history: <- S. Brauer, Dept. Microbiology, Cornell Univ., Ithaca, USA; 6A8 <- S. Brauer {2004}

NCBI tax ID(s): 358766 (species)

SI-ID 406415

DSM 21154

Section

Methanoregula boonei 6A8 is an anaerobe, mesophilic, Gram-negative archaeon that was isolated from acidic, ombrotrophic peat bog.

Gram-negative
rod-shaped
anaerobe
mesophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	"Methanomicrobia"
	Order	*Methanomicrobiales*
	Family	*Methanoregulaceae*
	Genus	*Methanoregula*
	Species	**Methanoregula boonei**
	Full Scientific Name (LPSN)	Methanoregula boonei Bräuer et al. 2011

Synonym

"Candidatus Methanoregula boonei"

Information on morphological and physiological properties Morphology

[Ref.: #29819]	Gram stain	negative

[Ref.: #29819]	Cell shape	rod-shaped

[Ref.: #29819]	Motility	no

[Ref.: #15567]

Incubation period

>14 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15567]

Culture medium

METHANOREGULA (PEAT) MEDIUM (DSMZ Medium 1280)

[Ref.: #15567]

Culture medium growth

[Ref.: #15567]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15567]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #15567]	growth	28 °C
[Ref.: #29819]	growth	10-40 °C
[Ref.: #29819]	optimum	36 °C

[Ref.: #15567]	Temperature range	mesophilic
[Ref.: #29819]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29819]			optimum	5.1
[Ref.: #29819]			growth	4.5-5.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #15567]	Oxygen tolerance	anaerobe
[Ref.: #29819]	Oxygen tolerance	anaerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29819]		NaCl		growth	0.002-0.145	%

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15567]	Sample type/isolated from	acidic, ombrotrophic peat bog
[Ref.: #15567]	Geographic location (country and/or sea, region)	New York, McLean bog near Ithaca
[Ref.: #15567]	Country	USA
[Ref.: #15567]	Country ISO 3 Code	USA
[Ref.: #15567]	Continent	North America
[Ref.: #15567]	Geographic location	42.5000°/-76.5000°
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Wetland (Swamp)
#Condition	#Acidic	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence DQ282124 (>99% sequence identity) for Methanoregula boonei subclade from Microbeatlas

Aquatic Samples	246
Soil Samples	120
Animal Samples
Plant Samples	10
Total Samples	376

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15567]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15567]	Candidatus Methanoregula boonei strain 6A8 16S ribosomal RNA gene, partial sequence; 16S-23S rRNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	DQ282124	1732	ENA	456442 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methanoregula boonei 6A8	GCA_000017625	complete	GenBank	456442 tax ID	*
[Ref.: #66792]	Methanoregula boonei 6A8	640753014	complete	JGI IMG/M	456442 tax ID	*
[Ref.: #66792]	Methanoregula boonei 6A8	456442.19	complete	PATRIC	456442 tax ID	*

[Ref.: #15567]	GC-content	54.5 mol%	sequence analysis
[Ref.: #29819]	GC-content	54.5 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methanoregula boonei 6A8 6A8 NCBI: GCA_000017625.1
[Ref.: #69480]	motile	no	91.335	no
[Ref.: #69480]	flagellated	no	96.957	yes
[Ref.: #69480]	gram-positive	no	96.69	yes
[Ref.: #69480]	anaerobic	yes	98.712	yes
[Ref.: #69480]	aerobic	no	95.122	yes
[Ref.: #69480]	halophile	no	96.285	yes
[Ref.: #69480]	spore-forming	no	95.671	no
[Ref.: #69480]	thermophile	no	86.761	yes
[Ref.: #69480]	glucose-util	yes	61.974	no
[Ref.: #69480]	glucose-ferment	no	64.71	no

Availability in culture collections External links

[Ref.: #15567]

Culture collection no.

DSM 21154, JCM 14090

[Ref.: #86847]

SI-ID 406415

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methanoregula boonei gen. nov., sp. nov., an acidiphilic methanogen isolated from an acidic peat bog.	Brauer SL, Cadillo-Quiroz H, Ward RJ, Yavitt JB, Zinder SH	Int J Syst Evol Microbiol	10.1099/ijs.0.021782-0	2010	*

References

#15567

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21154

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29819

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26196 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#86847

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID406415.1 )

#26196

IJSEM 45 2011 ( DOI 10.1099/ijs.0.021782-0 , PubMed 20154331 )

* These data were automatically processed and therefore are not curated

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Methanoregula boonei 6A8 6A8

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