Name: Akkermansia muciniphila Muc
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17849

Type strain:

Species: Akkermansia muciniphila

Strain Designation: Muc, MucT

Culture col. no.: DSM 22959, ATCC BAA-835, CIP 107961, CCUG 64013, JCM 33894, BCRC 81048

Strain history: CIP <- 2003, M. Derrien, Wageningen, The Netherlands: strain MucT

NCBI tax ID(s): 239935 (species)

SI-ID 134273

ATCC BAA-835, CIP 107961, CCUG 64013

Other strains from Akkermansia muciniphila

A. muciniphila YL44, DSM 26127
A. muciniphila JCM 30893

Section

Akkermansia muciniphila Muc is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from human intestinal tract .

Gram-negative
rod-shaped
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Verrucomicrobiota*
	Class	*Verrucomicrobiia*
	Order	*Verrucomicrobiales*
	Family	*Akkermansiaceae*
	Genus	*Akkermansia*
	Species	**Akkermansia muciniphila**
	Full Scientific Name (LPSN)	Akkermansia muciniphila Derrien et al. 2004

Information on morphological and physiological properties Morphology

[Ref.: #121158]	Gram stain	negative

[Ref.: #121158]	Cell shape	rod-shaped

[Ref.: #121158]	Motility	no

[Ref.: #16637]

Incubation period

3-7 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16637]

Culture medium

PYG MEDIUM (MODIFIED) (DSMZ Medium 104)

[Ref.: #16637]

Culture medium growth

[Ref.: #16637]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16637]

Culture medium composition

expand / minimize

[Ref.: #16637]

Culture medium

SCHAEDLER BROTH (ROTH; 5772) (DSMZ Medium 1669)

[Ref.: #16637]

Culture medium growth

[Ref.: #16637]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16637]

Culture medium composition

expand / minimize

[Ref.: #40737]

Culture medium

MEDIUM 187 - for anaerobic bacteria

[Ref.: #40737]

Culture medium growth

[Ref.: #40737]

Culture medium composition

expand / minimize

[Ref.: #121158]

Culture medium

CIP Medium 20

[Ref.: #121158]

Culture medium growth

[Ref.: #121158]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #121158]

Culture medium

CIP Medium 486

[Ref.: #121158]

Culture medium growth

[Ref.: #121158]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #121158]

Culture medium

CIP Medium 187

[Ref.: #121158]

Culture medium growth

[Ref.: #121158]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #16637]	growth	37 °C
[Ref.: #40737]	growth	37 °C
[Ref.: #63059]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #16637]	Temperature range	mesophilic
[Ref.: #40737]	Temperature range	mesophilic
[Ref.: #63059]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #16637]	Oxygen tolerance	anaerobe
[Ref.: #63059]	Oxygen tolerance	anaerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #121158]	nitrate ChEBI	-	reduction
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #121158]	nitrite ChEBI	-	reduction
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A

	Metabolite production	Metabolite	Production
[Ref.: #121158]		indole ChEBI	no
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	-	3.1.3.1	* from API rID32A, API zym
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A, API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68380]	alpha-glucosidase	-	3.2.1.20	* from API rID32A, API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	-	3.2.1.21	* from API rID32A, API zym
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A, API zym
[Ref.: #121158]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A, API zym
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #121158]	oxidase	-
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #121158]	urease	-	3.5.1.5
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #16637]	12936	-	-	+	+	-	-	-	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #16637]	12431	-	-	+	+	-	-	-	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #16637]	12432	-	-	+	+	-	-	-	-	-	+/-	-	-	+	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #16637]	12433	-	-	+/-	+	-	-	-	-	-	+	-	-	+	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #16637]	12434	-	-	+	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #16637]	12435	-	-	+	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #16637]	12436	-	-	+	+	-	-	-	-	-	+	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #16637]	12437	-	-	-	+/-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #16637]	12438	-	-	+	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #16637]	12101	-	-	+	+	-	-	-	-	-	+	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121158]	10779	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16637]	Sample type/isolated from	human intestinal tract (feces)
[Ref.: #16637]	Country	Netherlands
[Ref.: #16637]	Country ISO 3 Code	NLD
[Ref.: #16637]	Continent	Europe

[Ref.: #63059]	Sample type/isolated from	Human feces
[Ref.: #63059]	Sampling date	2002-03-01
[Ref.: #63059]	Country	Netherlands
[Ref.: #63059]	Country ISO 3 Code	NLD
[Ref.: #63059]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Human feces from a healthy adult volunteer
[Ref.: #67770]	Country	Netherlands
[Ref.: #67770]	Country ISO 3 Code	NLD
[Ref.: #67770]	Continent	Europe

[Ref.: #121158]	Sample type/isolated from	Human, Feces
[Ref.: #121158]	Country	Netherlands
[Ref.: #121158]	Country ISO 3 Code	NLD
[Ref.: #121158]	Continent	Europe
[Ref.: #121158]	Isolation date	2002
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY271254 (>99% sequence identity) for Akkermansia muciniphila subclade from Microbeatlas

Aquatic Samples	4383
Soil Samples	1656
Animal Samples	128875
Plant Samples	623
Total Samples	135537

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16637]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121158]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16637]	Akkermansia muciniphila strain Muc 16S ribosomal RNA gene, complete sequence	AY271254	1433	ENA	349741 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Akkermansia muciniphila ATCC BAA-835	GCA_000020225	complete	GenBank	349741 tax ID
[Ref.: #66792]	Akkermansia muciniphila DSM 22959	GCA_008000975	complete	GenBank	239935 tax ID	*
[Ref.: #66792]	Akkermansia muciniphila strain DSM 22959	239935.2131	complete	PATRIC	239935 tax ID	*

[Ref.: #16637]	GC-content	47.6 mol%
[Ref.: #16637]	GC-content	55.8 mol%	sequence analysis
[Ref.: #67770]	GC-content	47.6 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Akkermansia muciniphila DSM 22959 NCBI: GCA_008000975.1
[Ref.: #69480]	motile	no	90.651	no
[Ref.: #69480]	flagellated	no	97.163	no
[Ref.: #69480]	gram-positive	no	98.439	no
[Ref.: #69480]	anaerobic	yes	96.724	yes
[Ref.: #69480]	aerobic	no	94.053	yes
[Ref.: #69480]	halophile	no	92.474	no
[Ref.: #69480]	spore-forming	no	98.358	no
[Ref.: #69480]	glucose-util	yes	75.797	no
[Ref.: #69480]	thermophile	no	97.819	yes
[Ref.: #69480]	glucose-ferment	no	59.736	no

Availability in culture collections External links

[Ref.: #16637]

Culture collection no.

DSM 22959, ATCC BAA-835, CIP 107961, CCUG 64013, JCM 33894, BCRC 81048

[Ref.: #86840]

SI-ID 134273

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Akkermansia muciniphila and environmental enrichment reverse cognitive impairment associated with high-fat high-cholesterol consumption in rats.	Higarza SG, Arboleya S, Arias JL, Gueimonde M, Arias N	Gut Microbes	10.1080/19490976.2021.1880240	2021	*
Metabolism	Viability of microencapsulated Akkermansia muciniphila and Lactobacillus plantarum during freeze-drying, storage and in vitro simulated upper gastrointestinal tract passage.	Marcial-Coba MS, Cieplak T, Cahu TB, Blennow A, Knochel S, Nielsen DS	Food Funct	10.1039/c8fo01331d	2018	*
Phylogeny	Akkermansia muciniphila gen. nov., sp. nov., a human intestinal mucin-degrading bacterium.	Derrien M, Vaughan EE, Plugge CM, de Vos WM	Int J Syst Evol Microbiol	10.1099/ijs.0.02873-0	2004	*
Pathogenicity	Characterization of antibiotic-resistance traits in Akkermansia muciniphila strains of human origin.	Filardi R, Gargari G, Mora D, Arioli S	Sci Rep	10.1038/s41598-022-23980-6	2022	*
Phylogeny	Insights into the Antimicrobial Resistance Profile of a Next Generation Probiotic Akkermansia muciniphila DSM 22959.	Machado D, Barbosa JC, Almeida D, Andrade JC, Freitas AC, Gomes AM	Int J Environ Res Public Health	10.3390/ijerph19159152	2022	*
Metabolism	Genome analysis and 2'-fucosyllactose utilization characteristics of a new Akkermansia muciniphila strain isolated from mice feces.	Gao W, Xiao M, Gu Z, Fu X, Ren X, Yu Y, Liu Z, Zhu C, Kong Q, Mou H	Mol Genet Genomics	10.1007/s00438-022-01937-8	2022	*
	Preliminary Evaluation of the Safety and Probiotic Potential of Akkermansia muciniphila DSM 22959 in Comparison with Lactobacillus rhamnosus GG.	Cozzolino A, Vergalito F, Tremonte P, Iorizzo M, Lombardi SJ, Sorrentino E, Luongo D, Coppola R, Di Marco R, Succi M	Microorganisms	10.3390/microorganisms8020189	2020	*
	Spray-Drying Encapsulation of the Live Biotherapeutic Candidate Akkermansia muciniphila DSM 22959 to Survive Aerobic Storage.	Barbosa JC, Almeida D, Machado D, Sousa S, Freitas AC, Andrade JC, Gomes AM	Pharmaceuticals (Basel)	10.3390/ph15050628	2022	*

References

#16637

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22959

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#40737 ; Curators of the CIP;
#63059 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 64013

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68380

Automatically annotated from API rID32A .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#86840

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID134273.1 )

#121158 Collection of Institut Pasteur ; Curators of the CIP; CIP 107961
* These data were automatically processed and therefore are not curated

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Akkermansia muciniphila DSM 22959

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