Enzymology |
Molecular cloning, expression, purification, and characterization of fructose-1,6-bisphosphate aldolase from Thermus aquaticus. |
Sauve V, Sygusch J |
Protein Expr Purif |
10.1006/prep.2000.1380 |
2001 |
* |
Enzymology |
Crystallization and preliminary crystallographic analysis of an NADH oxidase that functions in peroxide reduction in Thermus aquaticus YT-1. |
Mac Sweeney A, D'Arcy A, Higgins TM, Mayhew SG, Toomey D, Walsh MA |
Acta Crystallogr D Biol Crystallogr |
10.1107/S090744499800941X |
1999 |
* |
Enzymology |
Purification and characterisation of NADH oxidase from Thermus aquaticus YT-1 and evidence that it functions in a peroxide-reduction system. |
Toomey D, Mayhew SG |
Eur J Biochem |
10.1046/j.1432-1327.1998.2510935.x |
1998 |
* |
Enzymology |
Cloning of genes of the aminopeptidase T family from Thermus thermophilus HB8 and Bacillus stearothermophilus NCIB8924: apparent similarity to the leucyl aminopeptidase family. |
Motoshima H, Minagawa E, Tsukasaki F, Kaminogawa S |
Biosci Biotechnol Biochem |
10.1271/bbb.61.1710 |
1997 |
* |
Enzymology |
Cloning and analysis of the DNA polymerase-encoding gene from Thermus caldophilus GK24. |
Kwon ST, Kim JS, Park JH, Kim HK, Lee DS |
Mol Cells |
|
1997 |
* |
Enzymology |
Molecular cloning and nucleotide sequence of the aminopeptidase T gene of Thermus aquaticus YT-1 and its high-level expression in Escherichia coli. |
Motoshima H, Azuma N, Kaminogawa S, Ono M, Minagawa E, Matsuzawa H, Ohta T, Yamauchi K |
Agric Biol Chem |
|
1990 |
* |
Enzymology |
Enhancement of the thermostability of subtilisin E by introduction of a disulfide bond engineered on the basis of structural comparison with a thermophilic serine protease. |
Takagi H, Takahashi T, Momose H, Inouye M, Maeda Y, Matsuzawa H, Ohta T |
J Biol Chem |
S0021-9258(19)39230-0 |
1990 |
* |
Enzymology |
NADH oxidase from the extreme thermophile Thermus aquaticus YT-1. Purification and characterisation. |
Cocco D, Rinaldi A, Savini I, Cooper JM, Bannister JV |
Eur J Biochem |
10.1111/j.1432-1033.1988.tb14093.x |
1988 |
* |
Metabolism |
The third restriction-modification system from Thermus aquaticus YT-1: solving the riddle of two TaqII specificities. |
Skowron PM, Anton BP, Czajkowska E, Zebrowska J, Sulecka E, Krefft D, Jezewska-Frackowiak J, Zolnierkiewicz O, Witkowska M, Morgan RD, Wilson GG, Fomenkov A, Roberts RJ, Zylicz-Stachula A |
Nucleic Acids Res |
10.1093/nar/gkx599 |
2017 |
* |
Enzymology |
Highly conserved salt bridge stabilizes a proteinase K subfamily enzyme, Aqualysin I, from Thermus aquaticus YT-1. |
Sakaguchi M, Osaku K, Maejima S, Ohno N, Sugahara Y, Oyama F, Kawakita M |
AMB Express |
10.1186/s13568-014-0059-2 |
2014 |
* |
Pathogenicity |
A novel exopolysaccharide from the biofilm of Thermus aquaticus YT-1 induces the immune response through Toll-like receptor 2. |
Lin MH, Yang YL, Chen YP, Hua KF, Lu CP, Sheu F, Lin GH, Tsay SS, Liang SM, Wu SH |
J Biol Chem |
10.1074/jbc.M110.200113 |
2011 |
* |
Phylogeny |
Thermus islandicus sp. nov., a mixotrophic sulfur-oxidizing bacterium isolated from the Torfajokull geothermal area. |
Bjornsdottir SH, Petursdottir SK, Hreggvidsson GO, Skirnisdottir S, Hjorleifsdottir S, Arnfinnsson J, Kristjansson JK |
Int J Syst Evol Microbiol |
10.1099/ijs.0.007013-0 |
2009 |
* |
Enzymology |
Stimulation of expression of a silica-induced protein (Sip) in Thermus thermophilus by supersaturated silicic acid. |
Doi K, Fujino Y, Inagaki F, Kawatsu R, Tahara M, Ohshima T, Okaue Y, Yokoyama T, Iwai S, Ogata S |
Appl Environ Microbiol |
10.1128/AEM.02387-08 |
2009 |
* |
Enzymology |
Construction of an expression system for aqualysin I in Escherichia coli that gives a markedly improved yield of the enzyme protein. |
Sakaguchi M, Niimiya K, Takezawa M, Toki T, Sugahara Y, Kawakita M |
Biosci Biotechnol Biochem |
10.1271/bbb.80132 |
2008 |
* |
Enzymology |
Role of disulphide bonds in a thermophilic serine protease aqualysin I from Thermus aquaticus YT-1. |
Sakaguchi M, Takezawa M, Nakazawa R, Nozawa K, Kusakawa T, Nagasawa T, Sugahara Y, Kawakita M |
J Biochem |
10.1093/jb/mvn007 |
2008 |
* |
Enzymology |
Role of proline residues in conferring thermostability on aqualysin I. |
Sakaguchi M, Matsuzaki M, Niimiya K, Seino J, Sugahara Y, Kawakita M |
J Biochem |
10.1093/jb/mvm025 |
2006 |
* |
Biotechnology |
Quantification of intra- and extra-cellular thermophilic lipase/esterase production by Thermus sp. |
Dominguez A, Sanroman A, Fucinos P, Rua ML, Pastrana L, Longo MA |
Biotechnol Lett |
10.1023/b:bile.0000024092.27943.75 |
2004 |
* |
Enzymology |
High-level expression, secretion, and purification of the thermostable aqualysin I from Thermus aquaticus YT-1 in Pichia pastoris. |
Oledzka G, Dabrowski S, Kur J |
Protein Expr Purif |
10.1016/s1046-5928(03)00060-3 |
2003 |
* |
Enzymology |
Independently expressed N-terminal pro-domain of aqualysin I precursor complements the folding of its mature domain to active form in Escherichia coli. |
Kim JY, Choi YL, Cho YS, Kim CH, Lee YC |
J Basic Microbiol |
10.1002/1521-4028(200206)42:3<181::AID-JOBM181>3.0.CO;2-8 |
2002 |
* |
Metabolism |
Requirement for the COOH-terminal pro-sequence in the translocation of aqualysin I across the cytoplasmic membrane in Escherichia coli. |
Kim DW, Matsuzawa H |
Biochem Biophys Res Commun |
10.1006/bbrc.2000.3657 |
2000 |
* |
Enzymology |
Weakly bound calcium ions involved in the thermostability of aqualysin I, a heat-stable subtilisin-type protease of Thermus aquaticus YT-1. |
Lin SJ, Yoshimura E, Sakai H, Wakagi T, Matsuzawa H |
Biochim Biophys Acta |
10.1016/s0167-4838(99)00140-5 |
1999 |
* |
Enzymology |
Identification and designing of the S3 site of aqualysin I, a thermophilic subtilisin-related serine protease. |
Tanaka T, Matsuzawa H, Ohta T |
J Biochem |
10.1093/oxfordjournals.jbchem.a022380 |
1999 |
* |
Enzymology |
Identification, cloning and expression of p25, an AT-rich DNA-binding protein from the extreme thermophile, Thermus aquaticus YT-1. |
Du X, Pene JJ |
Nucleic Acids Res |
10.1093/nar/27.7.1690 |
1999 |
* |
Enzymology |
Recombinant His-tagged DNA polymerase. II. Cloning and purification of Thermus aquaticus recombinant DNA polymerase (Stoffel fragment). |
Dabrowski S, Kur J |
Acta Biochim Pol |
|
1998 |
* |
Enzymology |
P1 specificity of aqualysin I (a subtilisin-type serine protease) from Thermus aquaticus YT-1, using P1-substituted derivatives of Streptomyces subtilisin inhibitor. |
Tanaka T, Matsuzawa H, Kojima S, Kumagai I, Miura K, Ohta T |
Biosci Biotechnol Biochem |
10.1271/bbb.62.2035 |
1998 |
* |
Metabolism |
Role of the COOH-terminal pro-sequence of aqualysin I (a heat-stable serine protease) in its extracellular secretion by Thermus thermophilus. |
Kim DW, Lee YC, Matsuzawa H |
FEMS Microbiol Lett |
10.1111/j.1574-6968.1997.tb12750.x |
1997 |
* |
Enzymology |
Stability of Thermostable Enzyme, Aqualysin I ; a Subtilisin-type Serine Protease from Thermus aquaticus YT-1. |
Tanaka T, Matsuzawa H, Ohta T |
Biosci Biotechnol Biochem |
10.1271/bbb.62.1806 |
1998 |
* |
Metabolism |
Identification and characterization of a thermostable MutS homolog from Thermus aquaticus. |
Biswas I, Hsieh P |
J Biol Chem |
10.1074/jbc.271.9.5040 |
1996 |
* |
Metabolism |
Thermophilic biodegradation of BTEX by two Thermus Species. |
Chen CI, Taylor RT |
Biotechnol Bioeng |
10.1002/bit.260480609 |
1995 |
* |
Enzymology |
Expression of aqualysin I (a thermophilic protease) in soluble form in Escherichia coli under a bacteriophage T7 promoter. |
Sakamoto S, Terada I, Iijima M, Ohta T, Matsuzawa H |
Biosci Biotechnol Biochem |
10.1271/bbb.59.1438 |
1995 |
* |
Enzymology |
A gene encoding a thermophilic alkaline serine proteinase from Thermus sp. strain Rt41A and its expression in Escherichia coli. |
Munro GK, McHale RH, Saul DJ, Reeves RA, Bergquist PL |
Microbiology (Reading) |
10.1099/13500872-141-7-1731 |
1995 |
* |
Enzymology |
Cloning and overexpression of thermostable DNA polymerase gene in Escherichia coli. |
Jin C, Liu H, Yang S, Zhang S |
Chin J Biotechnol |
|
1995 |
* |
Enzymology |
Fermentation conditions for efficient production of thermophilic protease in Escherichia coli harboring a plasmid. |
Sakamoto S, Terada I, Iijima M, Matsuzawa H, Ohta T |
Appl Microbiol Biotechnol |
10.1007/BF00173922 |
1994 |
* |
Enzymology |
Overproduction of Thermus aquaticus DNA polymerase and its structural analysis by ion-spray mass spectrometry. |
Ishino Y, Ueno T, Miyagi M, Uemori T, Imamura M, Tsunasawa S, Kato I |
J Biochem |
10.1093/oxfordjournals.jbchem.a124622 |
1994 |
* |
Enzymology |
Carboxypeptidase Taq, a thermostable zinc enzyme, from Thermus aquaticus YT-1: molecular cloning, sequencing, and expression of the encoding gene in Escherichia coli. |
Lee SH, Taguchi H, Yoshimura E, Minagawa E, Kaminogawa S, Ohta T, Matsuzawa H |
Biosci Biotechnol Biochem |
10.1271/bbb.58.1490 |
1994 |
* |
Enzymology |
The recA gene from the thermophile Thermus aquaticus YT-1: cloning, expression, and characterization. |
Angov E, Camerini-Otero RD |
J Bacteriol |
10.1128/jb.176.5.1405-1412.1994 |
1994 |
* |
Phylogeny |
Polar lipids and fatty acid composition of Thermus strains from New Zealand. |
Ferraz AS, Carreto L, Tenreiro S, Nobre MF, da Costa MS |
Antonie Van Leeuwenhoek |
10.1007/BF00882773 |
1994 |
* |
Enzymology |
Purification, crystallization and preliminary X-ray investigation of aqualysin I, a heat-stable serine protease. |
Green PR, Oliver JD, Strickland LC, Toerner DR, Matsuzawa H, Ohta T |
Acta Crystallogr D Biol Crystallogr |
10.1107/S0907444992012083 |
1993 |
* |
Enzymology |
Purification and characterization of a thermostable carboxypeptidase (carboxypeptidase Taq) from Thermus aquaticus YT-1. |
Lee SH, Minagawa E, Taguchi H, Matsuzawa H, Ohta T, Kaminogawa S, Yamauchi K |
Biosci Biotechnol Biochem |
10.1271/bbb.56.1839 |
1992 |
* |
Enzymology |
Involvement of NH2-terminal pro-sequence in the production of active aqualysin I (a thermophilic serine protease) in Escherichia coli. |
Lee YC, Miyata Y, Terada I, Ohta T, Matsuzawa H |
Agric Biol Chem |
|
1991 |
* |
Pathogenicity |
Production and Extracellular Secretion of Aqualysin I (a Thermophilic Subtilisin-Type Protease) in a Host-Vector System for Thermus thermophilus. |
Touhara N, Taguchi H, Koyama Y, Ohta T, Matsuzawa H |
Appl Environ Microbiol |
10.1128/aem.57.11.3385-3387.1991 |
1991 |
* |
Enzymology |
Molecular cloning and nucleotide sequence of 3-isopropylmalate dehydrogenase gene (leuB) from an extreme thermophile, Thermus aquaticus YT-1. |
Kirino H, Oshima T |
J Biochem |
10.1093/oxfordjournals.jbchem.a123470 |
1991 |
* |
Enzymology |
Unique precursor structure of an extracellular protease, aqualysin I, with NH2- and COOH-terminal pro-sequences and its processing in Escherichia coli. |
Terada I, Kwon ST, Miyata Y, Matsuzawa H, Ohta T |
J Biol Chem |
S0021-9258(19)39186-0 |
1990 |
* |
Enzymology |
Nucleotide sequence and characteristics of the gene for L-lactate dehydrogenase of Thermus aquaticus YT-1 and the deduced amino acid sequence of the enzyme. |
Ono M, Matsuzawa H, Ohta T |
J Biochem |
10.1093/oxfordjournals.jbchem.a123004 |
1990 |
* |
Enzymology |
Crystallization and preliminary X-ray diffraction studies of intact EF-Tu from Thermus aquaticus YT-1. |
Lippmann C, Betzel C, Dauter Z, Wilson K, Erdmann VA |
FEBS Lett |
10.1016/0014-5793(88)80355-7 |
1988 |
* |
Enzymology |
Determination of the positions of the disulfide bonds in aqualysin I (a thermophilic alkaline serine protease) of Thermus aquaticus YT-1. |
Kwon ST, Matsuzawa H, Ohta T |
J Biochem |
10.1093/oxfordjournals.jbchem.a122509 |
1988 |
* |
Enzymology |
Nucleotide sequence of the gene for aqualysin I (a thermophilic alkaline serine protease) of Thermus aquaticus YT-1 and characteristics of the deduced primary structure of the enzyme. |
Kwon ST, Terada I, Matsuzawa H, Ohta T |
Eur J Biochem |
10.1111/j.1432-1033.1988.tb14025.x |
1988 |
* |
Enzymology |
Purification and characterization of aqualysin I (a thermophilic alkaline serine protease) produced by Thermus aquaticus YT-1. |
Matsuzawa H, Tokugawa K, Hamaoki M, Mizoguchi M, Taguchi H, Terada I, Kwon ST, Ohta T |
Eur J Biochem |
10.1111/j.1432-1033.1988.tb13809.x |
1988 |
* |
Phylogeny |
Isolation and preliminary taxonomic studies of Thermus strains isolated from Yellowstone National Park, USA. |
Munster MJ, Munster AP, Woodrow JR, Sharp RJ |
J Gen Microbiol |
10.1099/00221287-132-6-1677 |
1986 |
* |
Enzymology |
Fructose 1,6-bisphosphate-dependent L-lactate dehydrogenase from Thermus aquaticus YT-1, an extreme thermophile: activation by citrate and modification reagents and comparison with Thermus caldophilus GK24 L-lactate dehydrogenase. |
Machida M, Matsuzawa H, Ohta T |
J Biochem |
10.1093/oxfordjournals.jbchem.a135132 |
1985 |
* |
Enzymology |
[Isolation and properties of DNA polymerase from the extreme thermophilic bacterium Thermus ruber]. |
Kaledin AS, Sliusarenko AG, Gorodetskii SI |
Biokhimiia |
|
1982 |
* |
Enzymology |
[Isolation and properties of DNA polymerase from extreme thermophylic bacteria Thermus aquaticus YT-1]. |
Kaledin AS, Sliusarenko AG, Gorodetskii SI |
Biokhimiia |
|
1980 |
* |
Metabolism |
Regulatory characteristics of phosphoenolpyruvate carboxylase from the extreme thermophile, Thermus aquaticus. |
Sundaram TK, Bridger GP |
Biochim Biophys Acta |
10.1016/0005-2744(79)90160-8 |
1979 |
* |
Metabolism |
An isochizomer of TaqI from Thermus thermophilus HB8. |
Sato S, Shinomiya T |
J Biochem |
10.1093/oxfordjournals.jbchem.a132252 |
1978 |
* |
Phylogeny |
A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus. |
Degryse E, Glansdorff N, Pierard A |
Arch Microbiol |
10.1007/BF00402307 |
1978 |
* |
|
Substrate Specificity of Aqualysin I Altered by an Organic Solvent, DMSO. |
Tanaka T, Matsuzawa H, Ohta T |
Biosci Biotechnol Biochem |
10.1271/bbb.63.446 |
1999 |
* |
|
Substrate Specificity of Aqualysin I, a Bacterial Thermophilic Alkaline Serine Protease from Thermus aquaticus YT-1: Comparison with Proteinase K, Subtilisin BPN' and Subtilisin Carlsberg. |
Tanaka T, Matsuzawa H, Ohta T |
Biosci Biotechnol Biochem |
10.1271/bbb.62.2161 |
1998 |
* |