Strain identifier BacDive ID:16461 Species:Streptomyces atriruber
Type strain:Yes Culture col. no.:DSM 41860, LDDC 6330-99, NRRL B-24165
  • Information on culture and growth conditionsCulture and growth conditionsarrow_down
  • [Ref.: #10641] Culture medium
    GYM STREPTOMYCES MEDIUM (DSMZ Medium 65), 28°C
    [Ref.: #10641] Culture medium link medium recipe provided by DSMZ
    [Ref.: #18726] Culture medium
    ISP 2
    [Ref.: #18726] Culture medium composition Name: ISP 2 / Yeast Malt Agar (5265); 5265
    Composition
    Malt extract 10.0 g/l
    Yeast extract 4.0 g/l
    Glucose 4.0 g/l
    Agar 15.0 g/l
    Preparation:
    Sterilisation: 20 minutes at 121°C
    pH before sterilisation: 7.0
    Usage: Maintenance and Taxonomy
    Organisms: All Actinomycetes
    [Ref.: #18726] Culture medium
    ISP 3
    [Ref.: #18726] Culture medium composition Name: ISP 3; 5315
    Composition
    Dog oat flakes 20.0 g/l
    Trace element solution (5314) 2.5 ml/l
    Agar 18.0 g/l
    Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated).
    Sterilisation: 20 minutes at 121°C
    pH before sterilisation: 7.8
    Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production)
    Organisms: All Actinomycetes

    Trace element solution 5314

    Name: Trace element solution 5314; 5314
    Composition
    CaCl2 x H2O 3.0 g/l
    Fe-III-citrate 1.0 g/l
    MnSO4 0.2 g/l
    ZnCl2 0.1 g/l
    CuSO4 x 5 H2O 0.025 g/l
    Sodium tetra borate 0.2 g/l
    CoCl2 x 6 H2O 0.004 g/l
    Sodium molybdate 0.01 g/l
    Preparation: Use double destillated water.
    Sterilisation: 20 minutes at 121°C
    pH before sterilisation:
    Usage: Trace element solution for different media
    Organisms:
    [Ref.: #18726] Culture medium
    ISP 4
    [Ref.: #18726] Culture medium composition Name: ISP 4; DSM 547

    Solution I:
    Difco soluble starch, 10.0 g.
    Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml.

    Solution II:
    CaCO3 2.0 g
    K2HPO4 (anhydrous) 1.0 g
    MgSO4 x 7 H2O 1.0 g
    NaCl 1.0 g
    (NH4)2SO4 2.0 g
    Distilled water 500.0 ml
    Trace salt solution (see below) 1.0 ml

    The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min.

    Trace element solution:
    FeSO4 x 7 H2O 0.1 g
    MnCl2 x 4 H2O 0.1 g
    ZnSO4 x 7 H2O 0.1 g
    Distilled water 100.0 ml
    [Ref.: #18726] Culture medium
    ISP 5
    [Ref.: #18726] Culture medium composition Name: ISP 5 (5323)
    Composition
    L-Asparagine 1.0 g/l
    Glycerol 10.0 g/l
    K2HPO4 1.0 g/l
    Salt solution (see preparation) 1.0 ml/l
    Agar 20.0 g/l
    Preparation:
    Salt solution
    1.0 g FeSO4 x 7 H2O
    1.0 g MnCl2 x 4 H2O
    1.0 g ZNSO4 x 7 H2O
    in 100 ml water
    Sterilisation:
    20 minutes at 121°C
    pH before sterilisation:
    7.2
    Usage:
    Maintenance and taxonomy
    Organisms:
    All Actinomycetes
    [Ref.: #18726] Culture medium
    ISP 7
    [Ref.: #18726] Culture medium composition Name: ISP 7 (5322)
    Composition
    Glycerol 15.0 g/l
    L-Tyrosine 0.5 g/l
    L-Asparagine 1.0 g/l
    K2HPO4 0.5 g/l
    NaCl 0.5 g/l
    FeSO4 x 7 H2O 0.01 g/l
    Trace element solution 5343 1.0 ml/l
    Agar 20.0
    Sterilisation:
    20 minutes at 121°C
    pH before sterilisation:
    7.3
    Usage:
    Production of melanoid pigments
    Organisms:
    All Actinomycetes
    Temperatures Kind of temperaturearrow to sort Temperaturearrow to sort
    [Ref.: #10641] optimum 28  ̊C
    [Ref.: #18726] optimum 28  ̊C
     
    [Ref.: #10641] Temperature range mesophilic