Name: Staphylococcus agnetis 6 VT
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 14677

Type strain:

Species: Staphylococcus agnetis

Strain Designation: 6 VT, 6-4

Culture col. no.: DSM 23656, CCUG 59809

Strain history: <- S. Taponen, University of Helsinki, Faculty of Veterinary Medicine; 6-4

NCBI tax ID(s): 985762 (species)

SI-ID 397173

DSM 23656, CCUG 59809

Other strains from Staphylococcus agnetis

S. agnetis 02-650, DSMZ: ID 09-1132, DSM 23657
S. agnetis 55 OE, 55-1, DSMZ: ID 09-1131, ...
S. agnetis CCUG 59810
S. agnetis CCUG 59811

Section

Staphylococcus agnetis 6 VT is a facultative anaerobe, mesophilic, Gram-positive animal pathogen that was isolated from bovine mastitic milk.

coccus-shaped
Gram-positive
animal pathogen
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Staphylococcaceae*
	Genus	*Staphylococcus*
	Species	**Staphylococcus agnetis**
	Full Scientific Name (LPSN)	Staphylococcus agnetis Taponen et al. 2012

Information on morphological and physiological properties Morphology

[Ref.: #30020]	Gram stain	positive

[Ref.: #30020]	Cell shape	coccus-shaped

[Ref.: #30020]	Motility	no

[Ref.: #17328]	Type of hemolysis	gamma
[Ref.: #17328]	Incubation period	1-2 days

[Ref.: #30020]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17328]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #17328]

Culture medium growth

[Ref.: #17328]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17328]

Culture medium composition

expand / minimize

[Ref.: #17328]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #17328]

Culture medium growth

[Ref.: #17328]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17328]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #17328]	growth	37 °C
[Ref.: #30020]	optimum	15-45 °C
[Ref.: #62115]	growth	37 °C

[Ref.: #17328]	Temperature range	mesophilic
[Ref.: #62115]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #30020]			optimum	6-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30020]	Oxygen tolerance	facultative anaerobe
[Ref.: #62115]	Oxygen tolerance	aerobe

[Ref.: #30020]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30020]		NaCl		growth	2-19	%

[Ref.: #30020]

Observation

aggregates in clumps

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68375]	arginine ChEBI	+	hydrolysis	* from API ID32STA
[Ref.: #68375]	D-fructose ChEBI	+	fermentation	* from API ID32STA
[Ref.: #68375]	D-glucose ChEBI	+	fermentation	* from API ID32STA
[Ref.: #68375]	D-mannose ChEBI	+	fermentation	* from API ID32STA
[Ref.: #68375]	esculin ChEBI	-	hydrolysis	* from API ID32STA
[Ref.: #30020]	fructose ChEBI	+	carbon source
[Ref.: #30020]	galactose ChEBI	+	carbon source
[Ref.: #30020]	glucose ChEBI	+	carbon source
[Ref.: #30020]	lactose ChEBI	+	carbon source
[Ref.: #68375]	lactose ChEBI	+	fermentation	* from API ID32STA
[Ref.: #68375]	maltose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #30020]	mannose ChEBI	+	carbon source
[Ref.: #30020]	melibiose ChEBI	+	carbon source
[Ref.: #68375]	N-acetylglucosamine ChEBI	+	fermentation	* from API ID32STA
[Ref.: #30020]	nitrate ChEBI	+	reduction
[Ref.: #68375]	nitrate ChEBI	+	reduction	* from API ID32STA
[Ref.: #68375]	ornithine ChEBI	-	degradation	* from API ID32STA
[Ref.: #68375]	raffinose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #30020]	ribose ChEBI	+	carbon source
[Ref.: #30020]	sucrose ChEBI	+	carbon source
[Ref.: #68375]	sucrose ChEBI	+	fermentation	* from API ID32STA
[Ref.: #30020]	trehalose ChEBI	+	carbon source
[Ref.: #68375]	trehalose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	urea ChEBI	-	hydrolysis	* from API ID32STA
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #68375]		novobiocin ChEBI	yes	1.8	µg	* from API ID32STA

	Metabolite production	Metabolite	Production
[Ref.: #68375]		acetoin ChEBI	no	* from API ID32STA

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #68375]		acetoin ChEBI	-	* from API ID32STA

	Enzyme	Enzyme activity	EC number
[Ref.: #68375]	arginine dihydrolase	+	3.5.3.6	* from API ID32STA
[Ref.: #68375]	beta-glucosidase	-	3.2.1.21	* from API ID32STA
[Ref.: #68375]	beta-glucuronidase	+	3.2.1.31	* from API ID32STA
[Ref.: #17328]	catalase	+	1.11.1.6
[Ref.: #30020]	catalase	+	1.11.1.6
[Ref.: #17328]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #68375]	L-arginine arylamidase	-		* from API ID32STA
[Ref.: #68375]	ornithine decarboxylase	-	4.1.1.17	* from API ID32STA
[Ref.: #68375]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API ID32STA
[Ref.: #68375]	urease	-	3.5.1.5	* from API ID32STA

API® ID32STA:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	Ornithine decarboxylaseODC	Esculin hydrolysisESC	Fermentation of D-glucoseGLU	Fermentation of D-fructoseFRU	Fermentation of D-mannoseMNE	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of trehaloseTRE	Fermentation of D-mannitolMAN	Fermentation of raffinoseRAF	Fermentation of D-riboseRIB	Fermentation of cellobioseCEL	Reduction of nitrateNIT	Acetoin production (Voges Proskauer test)VP	beta-Galactosidasebeta GAL	L-arginine arylamidaseArgA	Alkaline phosphatasePAL	Pyrrolidonyl arylamidasePyrA	Novobiocin resistanceNOVO	Fermentation of sucroseSAC	Fermentation of N-acetyl-glucosamineNAG	Fermentation of turanoseTUR	Fermentation of arabinoseARA	beta-Glucuronidasebeta GUR
[Ref.: #17328]	6179	-	+	-	-	+	+	+	-	+	-	-	-	-	-	+	-	-	-	-	-	-	+	+	-	-	+
[Ref.: #17328]	6100	-	+	-	-	+	+	+	-	+	-	+	-	+	+	+	-	+	-	+/-	-	-	+	+	+/-	+	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17328]	Sample type/isolated from	bovine mastitic milk
[Ref.: #17328]	Country	Finland
[Ref.: #17328]	Country ISO 3 Code	FIN
[Ref.: #17328]	Continent	Europe

[Ref.: #62115]	Sample type/isolated from	Bovine mastitis
[Ref.: #62115]	Geographic location (country and/or sea, region)	South
[Ref.: #62115]	Country	Finland
[Ref.: #62115]	Country ISO 3 Code	FIN
[Ref.: #62115]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body Product	#Fluids	#Milk

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HM484980 (>99% sequence identity) for Staphylococcus from Microbeatlas

Aquatic Samples	1
Soil Samples	1
Animal Samples	72
Plant Samples
Total Samples	74

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17328]	Pathogenicity (animal)	yes
[Ref.: #17328]	Biosafety level	2	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #17328]	Staphylococcus agnetis strain 6-4 16S ribosomal RNA gene, partial sequence	HM484980	1409	ENA	985762 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Staphylococcus agnetis DSM 23656	GCA_002901865	scaffold	GenBank	985762 tax ID	*
[Ref.: #66792]	Staphylococcus agnetis strain DSM 23656	985762.14	wgs	PATRIC	985762 tax ID	*

[Ref.: #17328]	GC-content	37.2 mol%	high performance liquid chromatography (HPLC)
[Ref.: #30020]	GC-content	37.2 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Staphylococcus agnetis DSM 23656 NCBI: GCA_002901865.1
[Ref.: #69480]	flagellated	no	93.704	yes
[Ref.: #69480]	gram-positive	yes	89.545	yes
[Ref.: #69480]	anaerobic	no	98.95	yes
[Ref.: #69480]	aerobic	yes	90.602	no
[Ref.: #69480]	halophile	yes	90.087	no
[Ref.: #69480]	spore-forming	no	89.086	yes
[Ref.: #69480]	glucose-util	yes	86.672	no
[Ref.: #69480]	thermophile	no	99.624	yes
[Ref.: #69480]	motile	no	90.975	yes
[Ref.: #69480]	glucose-ferment	yes	81.935	yes

Availability in culture collections External links

[Ref.: #17328]

Culture collection no.

DSM 23656, CCUG 59809

[Ref.: #83803]

SI-ID 397173

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Staphylococcus agnetis sp. nov., a coagulase-variable species from bovine subclinical and mild clinical mastitis.	Taponen S, Supre K, Piessens V, Van Coillie E, De Vliegher S, Koort JMK	Int J Syst Evol Microbiol	10.1099/ijs.0.028365-0	2011	*

References

#17328

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23656

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30020

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26384 (see below)

#62115 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 59809

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68375

Automatically annotated from API ID32STA .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#83803

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID397173.1 )

#26384

IJSEM 61 2012 ( DOI 10.1099/ijs.0.028365-0 , PubMed 21335502 )

* These data were automatically processed and therefore are not curated

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Staphylococcus agnetis DSM 23656

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