Name: Sterolibacterium denitrificans Chol-1S
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13907

Type strain:

Species: Sterolibacterium denitrificans

Strain Designation: Chol-1S

Culture col. no.: DSM 13999, ATCC BAA-354, Chol chol1, Chol 1

Strain history: <- S. Tarlera; chol1

NCBI tax ID(s): 157592 (species)

SI-ID 113529

DSM 13999, ATCC BAA-354

Section

Sterolibacterium denitrificans Chol-1S is an aerobe, mesophilic bacterium that was isolated from denitrifying reactor treating sanitary landfill leakate.

aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Nitrosomonadales*
	Family	*Sterolibacteriaceae*
	Genus	*Sterolibacterium*
	Species	**Sterolibacterium denitrificans**
	Full Scientific Name (LPSN)	Sterolibacterium denitrificans Tarlera and Denner 2003

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5211]

Culture medium

ANAEROBIC CHOLESTEROL MEDIUM (DSMZ Medium 858)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

R2A MEDIUM (DSMZ Medium 830)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #5211]			growth	30 °C

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Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #5211]	Oxygen tolerance	aerobe
[Ref.: #5211]	Oxygen tolerance	anaerobe

[Ref.: #5211]

Name of produced compound

ethylbenzene dehydrogenase

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5211]	Sample type/isolated from	denitrifying reactor treating sanitary landfill leakate
[Ref.: #5211]	Geographic location (country and/or sea, region)	Montevideo
[Ref.: #5211]	Country	Uruguay
[Ref.: #5211]	Country ISO 3 Code	URY
[Ref.: #5211]	Continent	Middle and South America
* marker position based on {}

Isolation sources categories

#Engineered	#Bioreactor	-
#Engineered	#Waste	#Landfill
#Engineered	#Waste	#Wastewater

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5211]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5211]	Sterolibacterium denitrificans 16S rRNA gene, strain Chol-1S	AJ306683	1531	ENA	157592 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Sterolibacterium denitrificans DSM 13999	GCA_001586935	scaffold	GenBank	157592 tax ID	*
[Ref.: #66792]	Sterolibacterium denitrificans strain DSM 13999	157592.11	wgs	PATRIC	157592 tax ID	*
[Ref.: #66792]	Sterolibacterium denitrificans DSM 13999	2690315849	draft	JGI IMG/M	157592 tax ID	*

[Ref.: #5211]

GC-content

65.3 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Sterolibacterium denitrificans DSM 13999 NCBI: GCA_001586935.1
[Ref.: #69480]	flagellated	no	73.342	no
[Ref.: #69480]	gram-positive	no	98.219	no
[Ref.: #69480]	anaerobic	no	66.327	no
[Ref.: #69480]	aerobic	no	72.482	no
[Ref.: #69480]	halophile	no	90.191	no
[Ref.: #69480]	spore-forming	no	97.097	no
[Ref.: #69480]	glucose-util	no	50	no
[Ref.: #69480]	motile	yes	89.741	no
[Ref.: #69480]	thermophile	no	92.137	yes
[Ref.: #69480]	glucose-ferment	no	71.358	no

Availability in culture collections External links

[Ref.: #5211]

Culture collection no.

DSM 13999, ATCC BAA-354, Chol chol1, Chol 1

[Ref.: #83082]

SI-ID 113529

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	The 7alpha-hydroxysteroid dehydratase Hsh2 is essential for anaerobic degradation of the steroid skeleton of 7alpha-hydroxyl bile salts in the novel denitrifying bacterium Azoarcus sp. strain Aa7.	Yucel O, Borgert SR, Poehlein A, Niermann K, Philipp B	Environ Microbiol	10.1111/1462-2920.14508	2019	*
Enzymology	Reaction mechanism of sterol hydroxylation by steroid C25 dehydrogenase - Homology model, reactivity and isoenzymatic diversity.	Rugor A, Wojcik-Augustyn A, Niedzialkowska E, Mordalski S, Staron J, Bojarski A, Szaleniec M	J Inorg Biochem	10.1016/j.jinorgbio.2017.04.027	2017	*
Enzymology	Optimization of overexpression of a chaperone protein of steroid C25 dehydrogenase for biochemical and biophysical characterization.	Niedzialkowska E, Mrugala B, Rugor A, Czub MP, Skotnicka A, Cotelesage JJH, George GN, Szaleniec M, Minor W, Lewinski K	Protein Expr Purif	10.1016/j.pep.2017.03.019	2017	*
Metabolism	Regioselective hydroxylation of cholecalciferol, cholesterol and other sterol derivatives by steroid C25 dehydrogenase.	Rugor A, Tataruch M, Staron J, Dudzik A, Niedzialkowska E, Nowak P, Hogendorf A, Michalik-Zym A, Napruszewska DB, Jarzebski A, Szymanska K, Bialas W, Szaleniec M	Appl Microbiol Biotechnol	10.1007/s00253-016-7880-2	2016	*
Phylogeny	A strictly anaerobic betaproteobacterium Georgfuchsia toluolica gen. nov., sp. nov. degrades aromatic compounds with Fe(III), Mn(IV) or nitrate as an electron acceptor.	Weelink SA, van Doesburg W, Saia FT, Rijpstra WI, Roling WF, Smidt H, Stams AJ	FEMS Microbiol Ecol	10.1111/j.1574-6941.2009.00778.x	2009	*
Metabolism	Study of anoxic and oxic cholesterol metabolism by Sterolibacterium denitrificans.	Chiang YR, Ismail W, Heintz D, Schaeffer C, Van Dorsselaer A, Fuchs G	J Bacteriol	10.1128/JB.01525-07	2007	*
Metabolism	Denitratisoma oestradiolicum gen. nov., sp. nov., a 17beta-oestradiol-degrading, denitrifying betaproteobacterium.	Fahrbach M, Kuever J, Meinke R, Kampfer P, Hollender J	Int J Syst Evol Microbiol	10.1099/ijs.0.63672-0	2006	*
Phylogeny	Sterolibacterium denitrificans gen. nov., sp. nov., a novel cholesterol-oxidizing, denitrifying member of the beta-Proteobacteria.	Tarlera S, Denner EBM	Int J Syst Evol Microbiol	10.1099/ijs.0.02039-0	2003	*

References

#5211

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13999

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#83082

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID113529.1 )

* These data were automatically processed and therefore are not curated

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Sterolibacterium denitrificans DSM 13999

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