Name: Thermithiobacillus plumbiphilus wk12
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132526

Type strain:

Species: Thermithiobacillus plumbiphilus

Strain Designation: wk12

Culture col. no.: DSM 101799, NBRC 111508

Strain history: <- T. Watanabe, Hokkaido University, Sapporo, Japan; wk12 <- T. Watanabe {2015}

NCBI tax ID(s): 1729899 (species)

SI-ID 408113

DSM 101799

Section

Thermithiobacillus plumbiphilus wk12 is a Gram-negative, motile, rod-shaped bacterium that was isolated from industrially synthesized lead sulfide.

Gram-negative
motile
rod-shaped
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Acidithiobacillia*
	Order	*Acidithiobacillales*
	Family	*Thermithiobacillaceae*
	Genus	*Thermithiobacillus*
	Species	**Thermithiobacillus plumbiphilus**
	Full Scientific Name (LPSN)	Thermithiobacillus plumbiphilus Watanabe et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43988]	Gram stain	negative

[Ref.: #43988]	Cell length	0.7-2.2 µm

[Ref.: #43988]	Cell width	0.5-1.0 µm

[Ref.: #43988]	Cell shape	rod-shaped

[Ref.: #43988]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24315]

Culture medium

SULFURIFERULA THIOPHILA MEDIUM (DSMZ Medium 1585a)

[Ref.: #24315]

Culture medium growth

[Ref.: #24315]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24315]

Culture medium composition

expand / minimize

[Ref.: #43988]

Culture medium

bicarbonate-buffered low-salt defined medium

[Ref.: #43988]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #24315]	growth	30 °C
[Ref.: #43988]	growth	5-37 °C
[Ref.: #43988]	optimum	28-32 °C

[Ref.: #24315]	Temperature range	mesophilic
[Ref.: #43988]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43988]			optimum	6.4-7.1
[Ref.: #43988]			growth	5.8-8.7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43988]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43988]		NaCl		optimum	0	M
[Ref.: #43988]		NaCl		growth	0-4-2.5

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #43988]		catalase	-	1.11.1.6
[Ref.: #43988]		cytochrome oxidase	-	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24315]	Sample type/isolated from	industrially synthesized lead (II) sulfide
[Ref.: #24315]	Country	Japan
[Ref.: #24315]	Country ISO 3 Code	JPN
[Ref.: #24315]	Continent	Asia

[Ref.: #43988]	Sample type/isolated from	industrially synthesized lead (II) sulfide
[Ref.: #43988]	Enrichment culture	modified ATCC 290 S6 medium
[Ref.: #43988]	Enrichment culture composition	3 g/L Na2HPO4.12H2O, 1.8 g/L KH2PO4, 0.1 g/L MgSO4.7H2O, 0.1 g/L (NH4)2SO4, 0.04 g/L CaCl2 . 2H2O, 0.03 g FeCl3.6H2O, 0.03 g/L MnSO4.5H2O, 10 g/L Na2S2O3, with decreased thiosulfate concentration to 0.4 mM
[Ref.: #43988]	Enrichment culture temperature	22 ̊C
* marker position based on {}

Isolation sources categories

#Engineered

#Industrial

#Industrial production

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC088006 (>99% sequence identity) for Thermithiobacillus plumbiphilus from Microbeatlas

Aquatic Samples	116
Soil Samples	276
Animal Samples	15
Plant Samples	14
Total Samples	421

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24315]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24315]	Thermithiobacillus plumbiphilus gene for 16S ribosomal RNA, partial sequence	LC088006	1419	ENA	1729899 tax ID

[Ref.: #24315]

GC-content

58.5 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #24315]

Culture collection no.

DSM 101799, NBRC 111508

[Ref.: #90852]

SI-ID 408113

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Thermithiobacillus plumbiphilus sp. nov., a sulfur-oxidizing bacterium isolated from lead sulfide.	Watanabe T, Miura A, Shinohara A, Kojima H, Fukui M	Int J Syst Evol Microbiol	10.1099/ijsem.0.000972	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24315

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 101799

#43988

Tomohiro Watanabe, Aya Miura, Arisa Shinohara, Hisaya Kojima, Manabu Fukui: Thermithiobacillus plumbiphilus sp. nov., a sulfur-oxidizing bacterium isolated from lead sulfide. IJSEM 66: 1986 - 1989 2016 ( DOI 10.1099/ijsem.0.000972 , PubMed 26873326 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#90852

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID408113.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Thermithiobacillus plumbiphilus in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF