Name: Pseudomonas otitidis DSM 17224
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 13113

Type strain:

Species: Pseudomonas otitidis

Culture col. no.: DSM 17224, ATCC BAA-1130, CIP 109236, MCC 10330, CCUG 55592

Strain history: CIP <- 2006, DSMZ <- D.W. Stroman, Alcon Res., Fort Worth, Texas, USA: strain MCC 10330

NCBI tax ID(s): 319939 (species)

SI-ID 290994

ATCC BAA-1130, DSM 17224, LMG 23769, CIP 109236, CCUG 55592

Other strains from Pseudomonas otitidis

P. otitidis CCUG 70506

Section

Pseudomonas otitidis DSM 17224 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from ear of patient with acute Otitis externa.

Gram-negative
motile
rod-shaped
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas otitidis**
	Full Scientific Name (LPSN)	Pseudomonas otitidis Clark et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #121469]	Gram stain	negative

[Ref.: #121469]	Cell shape	rod-shaped

[Ref.: #121469]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6824]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #6824]

Culture medium growth

[Ref.: #6824]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #6824]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #6824]

Culture medium growth

[Ref.: #6824]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #37488]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37488]

Culture medium growth

[Ref.: #37488]

Culture medium composition

expand / minimize

[Ref.: #121469]

Culture medium

CIP Medium 72

[Ref.: #121469]

Culture medium growth

[Ref.: #121469]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #121469]

Culture medium

CIP Medium 566

[Ref.: #121469]

Culture medium growth

[Ref.: #121469]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #6824]			growth	37 °C
[Ref.: #37488]			growth	37 °C

[Ref.: #6824]	Temperature range	mesophilic
[Ref.: #37488]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #121469]

Oxygen tolerance

obligate aerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #121469]	nitrate ChEBI	-	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #121469]	nitrite ChEBI	-	reduction
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #121469]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	+	3.5.3.6	* from API 20NE
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE, API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121469]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121469]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121469]	urease	+	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #6824]	25278	-	-	-	+	-	-	+	-	+	-	-	-	-	-	-	+	-	+	+	+	+
[Ref.: #6824]	22889	-	-	-	+	-	-	+	-	+	-	-	-	-	-	-	+	-	+	+	+	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121469]	11737	-	+	+	+	+	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6824]	Sample type/isolated from	ear of patient with acute Otitis externa
[Ref.: #6824]	Country	USA
[Ref.: #6824]	Country ISO 3 Code	USA
[Ref.: #6824]	Continent	North America

[Ref.: #121469]	Sample type/isolated from	Human, Ear
* marker position based on {}

Isolation sources categories

#Infection	#Disease	-
#Infection	#Inflammation	-
#Infection	#Patient	-
#Host Body-Site	#Organ	#Ear

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6824]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #121469]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6824]	Pseudomonas otitidis 16S ribosomal RNA gene, partial sequence	AY953147	1531	ENA	319939 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudomonas otitidis DSM 17224	GCA_900111835	scaffold	GenBank	319939 tax ID	*
[Ref.: #66792]	Pseudomonas otitidis strain DSM 17224	319939.11	wgs	PATRIC	319939 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudomonas otitidis DSM 17224 NCBI: GCA_900111835.1
[Ref.: #69480]	motile	yes	90.988	no
[Ref.: #69480]	flagellated	yes	85.217	no
[Ref.: #69480]	gram-positive	no	98.677	no
[Ref.: #69480]	anaerobic	no	98.139	no
[Ref.: #69480]	aerobic	yes	95.695	no
[Ref.: #69480]	halophile	no	88.881	no
[Ref.: #69480]	spore-forming	no	93.352	no
[Ref.: #69480]	glucose-ferment	no	91.173	yes
[Ref.: #69480]	thermophile	no	99.777	no
[Ref.: #69480]	glucose-util	yes	91.277	yes

Availability in culture collections External links

[Ref.: #6824]

Culture collection no.

DSM 17224, ATCC BAA-1130, CIP 109236, MCC 10330, CCUG 55592

[Ref.: #82321]

SI-ID 290994

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudomonas tohonis sp. nov., isolated from the skin of a patient with burn wounds in Japan.	Yamada K, Sasaki M, Aoki K, Nagasawa T, Murakami H, Ishii M, Shibuya K, Morita T, Ishii Y, Tateda K	Int J Syst Evol Microbiol	10.1099/ijsem.0.005115	2021	*
Genetics	Complete Genome Sequence of Pseudomonas otitidis Strain MrB4, Isolated from Lake Biwa in Japan.	Miyazaki K, Hase E, Maruya T	Microbiol Resour Announc	10.1128/MRA.00148-20	2020	*
Phylogeny	Metallo-beta-lactamase production by Pseudomonas otitidis: a species-related trait.	Thaller MC, Borgianni L, Di Lallo G, Chong Y, Lee K, Dajcs J, Stroman D, Rossolini GM	Antimicrob Agents Chemother	10.1128/AAC.01062-10	2010	*
Phylogeny	Pseudomonas otitidis sp. nov., isolated from patients with otic infections.	Clark LL, Dajcs JJ, McLean CH, Bartell JG, Stroman DW	Int J Syst Evol Microbiol	10.1099/ijs.0.63753-0	2006	*

References

#6824

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17224

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#37488 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#82321

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID290994.1 )

#121469 Collection of Institut Pasteur ; Curators of the CIP; CIP 109236
* These data were automatically processed and therefore are not curated

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