Name: Pseudomonas citronellolis DSM 50332
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 12823

Type strain:

Species: Pseudomonas citronellolis

Culture col. no.: DSM 50332, ATCC 13674, CIP 104381, CCUG 17933, LMG 18378, NRRL B-2504, CFBP 5585, JCM 13426, BCRC 14345, IAM 15129, JCM 21587, KACC 10776, KCTC 12148, NBRC 103043, NCIMB 12783

Strain history: CIP <- 1995, DSM <- ATCC <- W. Seubert

NCBI tax ID(s): 1215097 (strain), 53408 (species)

SI-ID 11919

LMG 18378, ATCC 13674, CIP 104381, DSM 50332, NCIMB 12783, CCUG 17933, BCRC 14345, CCRC 14345, CCRC 13907, NRRL B-2504, CFBP 5585, KCTC 12148, KACC 10776, IAM 15129, JCM 21587, NBRC 103043

Other strains from Pseudomonas citronellolis

P. citronellolis ADP, DSM 11735, CCUG 54931
P. citronellolis D21, DSM 13132
P. citronellolis D 26, DSM 13957
P. citronellolis 6903, BG 6903, DSM 18650, CCUG ...
P. citronellolis CCUG 42982
P. citronellolis CCUG 53022
P. citronellolis CCUG 53068
P. citronellolis CCUG 59066
P. citronellolis CCUG 64306 A
P. citronellolis CCUG 64909
P. citronellolis CCUG 70659
P. citronellolis JCM 33429

Section

Pseudomonas citronellolis DSM 50332 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from soil under pine trees.

Gram-negative
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas citronellolis**
	Full Scientific Name (LPSN)	Pseudomonas citronellolis Seubert 1960 (Approved Lists 1980)

Synonym

Pseudomonas humi

Information on morphological and physiological properties Morphology

[Ref.: #118305]	Gram stain	negative

[Ref.: #118305]	Cell shape	rod-shaped

[Ref.: #118305]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #12711]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #12711]

Culture medium growth

[Ref.: #12711]

Culture medium link

Medium recipe at MediaDive

[Ref.: #12711]

Culture medium composition

expand / minimize

[Ref.: #40147]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #40147]

Culture medium growth

[Ref.: #40147]

Culture medium composition

expand / minimize

[Ref.: #118305]

Culture medium

CIP Medium 3

[Ref.: #118305]

Culture medium growth

[Ref.: #118305]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #118305]

Culture medium

CIP Medium 72

[Ref.: #118305]

Culture medium growth

[Ref.: #118305]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #12711]	growth	30 °C
[Ref.: #40147]	growth	30 °C
[Ref.: #46720]	growth	30 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #12711]	Temperature range	mesophilic
[Ref.: #40147]	Temperature range	mesophilic
[Ref.: #46720]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #46720]	Oxygen tolerance	aerobe
[Ref.: #118305]	Oxygen tolerance	obligate aerobe

[Ref.: #12711]	Name of produced compound	polyhydroxyalkanoic acids
[Ref.: #12711]	Name of produced compound	isoprene

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #118305]	nitrate ChEBI	+	reduction
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #118305]	nitrite ChEBI	+	reduction
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #118305]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	+	3.5.3.6	* from API 20NE
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE, API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #118305]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #118305]	oxidase	+
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #118305]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #12711]	23042	+	-	-	+	-	-	-	-	+	-	-	-	-	-	+	+	-	+	+	+	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #118305]	7918	-	+	+	+	-	+	-	-	+	-	+	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #12711]	Sample type/isolated from	soil under pine trees
[Ref.: #12711]	Geographic location (country and/or sea, region)	Virginia
[Ref.: #12711]	Country	USA
[Ref.: #12711]	Country ISO 3 Code	USA
[Ref.: #12711]	Continent	North America

[Ref.: #46720]	Sample type/isolated from	Soil under pine trees
[Ref.: #46720]	Country	USA
[Ref.: #46720]	Country ISO 3 Code	USA
[Ref.: #46720]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Soil under pine trees in northern Virginia
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America

[Ref.: #118305]	Sample type/isolated from	Environment, Soil under pine trees
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Tree

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence Z76659 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	31
Soil Samples	16
Animal Samples	53
Plant Samples	12
Total Samples	112

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #12711]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118305]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #12711]	P.citronellolis 16S rRNA gene	Z76659	1510	ENA	53408 tax ID
[Ref.: #67770]	Pseudomonas citronellolis gene for 16S rRNA, partial sequence, strain: NBRC 103043	AB681923	1462	ENA	53408 tax ID
[Ref.: #20218]	Pseudomonas citronellolis gene for 16S rRNA, strain: ATCC 13674	AB021396	1499	ENA	53408 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Pseudomonas citronellolis NBRC 103043	GCA_002091555	contig	GenBank	1215097 tax ID
[Ref.: #67770]	Pseudomonas citronellolis LMG 18378	GCA_900112375	scaffold	GenBank	53408 tax ID
[Ref.: #67770]	Pseudomonas citronellolis DSM 50332	GCA_004745455	contig	GenBank	53408 tax ID
[Ref.: #66792]	Pseudomonas citronellolis NBRC 103043 strain NBRC 103043	1215097.3	wgs	PATRIC	1215097 tax ID	*
[Ref.: #66792]	Pseudomonas citronellolis strain DSM 50332	53408.24	wgs	PATRIC	53408 tax ID	*
[Ref.: #66792]	Pseudomonas citronellolis strain LMG 18378	53408.9	wgs	PATRIC	53408 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudomonas citronellolis NBRC 103043 strain NBRC 103043 PATRIC: 1215097.3
[Ref.: #69480]	motile	yes	90.003	no
[Ref.: #69480]	gram-positive	no	98.353	no
[Ref.: #69480]	anaerobic	no	97.689	yes
[Ref.: #69480]	aerobic	yes	89.698	yes
[Ref.: #69480]	halophile	no	78.733	no
[Ref.: #69480]	spore-forming	no	94.889	no
[Ref.: #69480]	thermophile	no	99.702	yes
[Ref.: #69480]	glucose-util	yes	90.172	yes
[Ref.: #69480]	flagellated	yes	72.925	no
[Ref.: #69480]	glucose-ferment	no	89.272	yes

Availability in culture collections External links

[Ref.: #12711]

Culture collection no.

DSM 50332, ATCC 13674, CIP 104381, CCUG 17933, LMG 18378, NRRL B-2504, CFBP 5585, JCM 13426, BCRC 14345, IAM 15129, JCM 21587, KACC 10776, KCTC 12148, NBRC 103043, NCIMB 12783

[Ref.: #82035]

SI-ID 11919

Literature:

Only first 10 entries are displayed. Click here to see all.

	Title	Authors	Journal	DOI	Year
Metabolism	Potential for mcl-PHA production from nonanoic and azelaic acids.	Gillis J, Ko K, Ramsay JA, Ramsay BA	Can J Microbiol	10.1139/cjm-2017-0554	2017	*
Phylogeny	Pseudomonas panipatensis sp. nov., isolated from an oil-contaminated site.	Gupta SK, Kumari R, Prakash O, Lal R	Int J Syst Evol Microbiol	10.1099/ijs.0.65401-0	2008	*
Phylogeny	Pseudomonas knackmussii sp. nov.	Stolz A, Busse HJ, Kampfer P	Int J Syst Evol Microbiol	10.1099/ijs.0.64761-0	2007	*
Phylogeny	Pseudomonas delhiensis sp. nov., from a fly ash dumping site of a thermal power plant.	Prakash O, Kumari K, Lal R	Int J Syst Evol Microbiol	10.1099/ijs.0.64456-0	2007	*
Metabolism	Pyrimidine nucleotide synthesis in Pseudomonas citronellolis.	West TP	Can J Microbiol	10.1139/w04-028	2004	*
Enzymology	Molecular cloning of the carboxylesterase gene and biochemical characterization of the encoded protein from Pseudomonas citronellolis ATCC 13674.	Chao YP, Fu H, Wang YL, Huang WB, Wang JY	Res Microbiol	10.1016/S0923-2508(03)00144-X	2003	*
Phylogeny	Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea.	Kwon SW, Kim JS, Park IC, Yoon SH, Park DH, Lim CK, Go SJ	Int J Syst Evol Microbiol	10.1099/ijs.0.02326-0	2003	*
Metabolism	Intracellular degradation of two structurally different polyhydroxyalkanoic acids accumulated in Pseudomonas putida and Pseudomonas citronellolis from mixtures of octanoic acid and 5-phenylvaleric acid.	Chung DM, Choi MH, Song JJ, Yoon SC, Kang IK, Huh NE	Int J Biol Macromol	10.1016/s0141-8130(01)00172-6	2001	*
Metabolism	Microbial degradation of monoterpenes in the absence of molecular oxygen.	Harder J, Probian C	Appl Environ Microbiol	10.1128/aem.61.11.3804-3808.1995	1995	*
Phylogeny	Pseudomonas humi sp. nov., isolated from leaf soil.	Akita H, Kimura ZI, Hoshino T	Arch Microbiol	10.1007/s00203-018-1588-x	2018	*
	Preparation and Characterization of Films Based on a Natural P(3HB)/mcl-PHA Blend Obtained through the Co-culture of Cupriavidus Necator and Pseudomonas Citronellolis in Apple Pulp Waste.	Rebocho AT, Pereira JR, Neves LA, Alves VD, Sevrin C, Grandfils C, Freitas F, Reis MAM	Bioengineering (Basel)	10.3390/bioengineering7020034	2020	*
	Polyester Biosynthesis Characteristics of Pseudomonas citronellolis Grown on Various Carbon Sources, Including 3-Methyl-Branched Substrates.	Choi MH, Yoon SC	Appl Environ Microbiol	10.1128/aem.60.9.3245-3254.1994	1994	*

References

#12711

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 50332

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40147 ; Curators of the CIP;
#46720 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 17933

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#82035

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID11919.1 )

#118305 Collection of Institut Pasteur ; Curators of the CIP; CIP 104381
* These data were automatically processed and therefore are not curated

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Pseudomonas citronellolis NBRC 103043 strain NBRC 103043

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