Name: Actinobacillus succinogenes 130Z
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11687

Type strain:

Species: Actinobacillus succinogenes

Strain Designation: 130Z

Culture col. no.: DSM 22257, ATCC 55618, CCUG 43843, CIP 106512

Strain history: CIP <- 2000, ATCC <- Michigan Biotechnol. Inst., USA: Strain 130Z

NCBI tax ID(s): 67854 (species)

SI-ID 43634

ATCC 55618, CIP 106512, CCUG 43843, DSM 22257

Section

Actinobacillus succinogenes 130Z is an aerobe, mesophilic, Gram-negative bacterium that was isolated from cattle rumen.

Gram-negative
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pasteurellales*
	Family	*Pasteurellaceae*
	Genus	*Actinobacillus*
	Species	**Actinobacillus succinogenes**
	Full Scientific Name (LPSN)	Actinobacillus succinogenes Guettler et al. 1999

Information on morphological and physiological properties Morphology

[Ref.: #123367]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.958

[Ref.: #123367]	Cell shape	rod-shaped

[Ref.: #123367]	Motility	no

[Ref.: #16239]

Incubation period

1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16239]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #16239]

Culture medium growth

[Ref.: #16239]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16239]

Culture medium composition

expand / minimize

[Ref.: #16239]

Culture medium

BHI MEDIUM (DSMZ Medium 215)

[Ref.: #16239]

Culture medium growth

[Ref.: #16239]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16239]

Culture medium composition

expand / minimize

[Ref.: #41680]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #41680]

Culture medium growth

[Ref.: #41680]

Culture medium composition

expand / minimize

[Ref.: #123367]

Culture medium

CIP Medium 72

[Ref.: #123367]

Culture medium growth

[Ref.: #123367]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #123367]

Culture medium

CIP Medium 3

[Ref.: #123367]

Culture medium growth

[Ref.: #123367]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #16239]		growth	37 °C
[Ref.: #41680]		growth	30 °C
[Ref.: #56056]		growth	30-37 °C
[Ref.: #123367]		growth	25-37 °C
[Ref.: #123367]	no	growth	15 °C
[Ref.: #123367]	no	growth	41 °C
[Ref.: #123367]	no	growth	45 °C

[Ref.: #16239]	Temperature range	mesophilic
[Ref.: #41680]	Temperature range	mesophilic
[Ref.: #56056]	Temperature range	mesophilic
[Ref.: #123367]	Temperature range	mesophilic
[Ref.: #123367]	Temperature range	psychrophilic
[Ref.: #123367]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #56056]	Oxygen tolerance	aerobe
[Ref.: #123367]	Oxygen tolerance	obligate aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.996

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68377]	D-glucose ChEBI	+	builds acid from	* from API NH
[Ref.: #123367]	esculin ChEBI	-	hydrolysis
[Ref.: #123367]	hippurate ChEBI	+	hydrolysis
[Ref.: #123367]	nitrate ChEBI	+	reduction
[Ref.: #123367]	nitrite ChEBI	+	reduction
[Ref.: #68377]	ornithine ChEBI	-	degradation	* from API NH

	Metabolite production	Metabolite	Production
[Ref.: #123367]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #123367]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #123367]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #123367]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #16239]	catalase	+	1.11.1.6
[Ref.: #123367]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #16239]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #123367]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #123367]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #123367]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68377]	ornithine decarboxylase	-	4.1.1.17	* from API NH
[Ref.: #123367]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #123367]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #123367]	1729	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® NH:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Penicillinase productionPEN	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from maltoseMAL	Acid from sucroseSAC	Ornithine decarboxylaseODC	Urease/urea hydrolysisURE	LipaseLIP	Alkaline phosphatasePAL	beta-Galactosidasebeta GAL	Proline arylamidaseProA	gamma-Glutamyl transferaseGGT	Indole productionIND
[Ref.: #16239]	6162	+	+	+	+	-	-	+	-	+	-	+	-	-
[Ref.: #16239]	6161	+	+	-	+	-	-	+	+/-	+	-	+	-	-
[Ref.: #16239]	6160	+	+	+	+	-	-	+	+	+	-	+	-	+
[Ref.: #16239]	6159	+	+	+	+	-	-	+	+	+	-	+	-	-
[Ref.: #16239]	5973	-	+	+	+	+	-	-	+	-	+	-	+	-
[Ref.: #16239]	5713	-	+	+	+	+	-	-	+	-	+	-	+	-
[Ref.: #16239]	5712	-	+	+	-	+	-	-	+	+/-	+	-	+	-
[Ref.: #16239]	5711	-	+	+	+	+	-	-	+	+	+	-	+	-
[Ref.: #16239]	5710	+	+	+	+	+	-	-	+	+	+	-	+	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123367]	9570	-	+	+	+	-	+	-	-	-	-	+	+	-	+	-	-	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16239]	Sample type/isolated from	cattle rumen

[Ref.: #56056]	Sample type/isolated from	Cattle rumen
[Ref.: #56056]	Geographic location (country and/or sea, region)	Michigan
[Ref.: #56056]	Country	USA
[Ref.: #56056]	Country ISO 3 Code	USA
[Ref.: #56056]	Continent	North America

[Ref.: #123367]	Sample type/isolated from	Animal, Cattle, rumen contents
[Ref.: #123367]	Geographic location (country and/or sea, region)	Michigan
[Ref.: #123367]	Country	United States of America
[Ref.: #123367]	Country ISO 3 Code	USA
[Ref.: #123367]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body-Site	#Organ	#Rumen

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AF024525 (>99% sequence identity) for Actinobacillus succinogenes subclade from Microbeatlas

Aquatic Samples	160
Soil Samples	52
Animal Samples	1616
Plant Samples	11
Total Samples	1839

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16239]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123367]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16239]	Actinobacillus succinogenes 16S ribosomal RNA gene, complete sequence	AF024525	1488	ENA	67854 tax ID
[Ref.: #20218]	Actinobacillus succinogenes strain CCUG 43843 16S ribosomal RNA gene, partial sequence	AY362898	1365	ENA	67854 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Actinobacillus succinogenes 130Z	339671.7	complete	PATRIC	339671 tax ID	*
[Ref.: #66792]	Actinobacillus succinogenes 130Z	640753001	complete	JGI IMG/M	339671 tax ID	*

[Ref.: #16239]

GC-content

45.0 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Actinobacillus succinogenes 130Z PATRIC: 339671.7
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinobacillus succinogenes 130Z PATRIC: 339671.7
[Ref.: #69480]	gram-positive	no	97.955	no
[Ref.: #69480]	anaerobic	no	91.543	yes
[Ref.: #69480]	halophile	no	53.29	no
[Ref.: #69480]	spore-forming	no	98.843	no
[Ref.: #69480]	glucose-util	yes	51.581	no
[Ref.: #69480]	motile	no	89.409	no
[Ref.: #69480]	flagellated	no	95.639	no
[Ref.: #69480]	aerobic	no	96.248	no
[Ref.: #69480]	thermophile	no	99.331	yes
[Ref.: #69480]	glucose-ferment	yes	63.035	no

Availability in culture collections External links

[Ref.: #16239]

Culture collection no.

DSM 22257, ATCC 55618, CCUG 43843, CIP 106512

[Ref.: #80907]

SI-ID 43634

Literature:

Only first 10 entries are displayed. Click here to see all.

	Title	Authors	Journal	DOI	Year
Enzymology	Continuous Succinic Acid Fermentation by Actinobacillus Succinogenes: Assessment of Growth and Succinic Acid Production Kinetics.	Ferone M, Raganati F, Olivieri G, Salatino P, Marzocchella A	Appl Biochem Biotechnol	10.1007/s12010-018-2846-8	2018	*
Metabolism	Biosuccinic Acid from Lignocellulosic-Based Hexoses and Pentoses by Actinobacillus succinogenes: Characterization of the Conversion Process.	Ferone M, Raganati F, Olivieri G, Salatino P, Marzocchella A	Appl Biochem Biotechnol	10.1007/s12010-017-2514-4	2017	*
Phylogeny	Actinobacillus succinogenes ATCC 55618 fermentation medium optimization for the production of succinic acid by response surface methodology.	Zhu LW, Wang CC, Liu RS, Li HM, Wan DJ, Tang YJ	J Biomed Biotechnol	10.1155/2012/626137	2012	*
Metabolism	Simultaneous saccharification and fermentation of acid-pretreated rapeseed meal for succinic acid production using Actinobacillus succinogenes.	Chen K, Zhang H, Miao Y, Wei P, Chen J	Enzyme Microb Technol	10.1016/j.enzmictec.2010.12.009	2010	*
Metabolism	Significance of CO2 donor on the production of succinic acid by Actinobacillus succinogenes ATCC 55618.	Zou W, Zhu LW, Li HM, Tang YJ	Microb Cell Fact	10.1186/1475-2859-10-87	2011	*
Metabolism	Performance analyses of a neutralizing agent combination strategy for the production of succinic acid by Actinobacillus succinogenes ATCC 55618.	Wang CC, Zhu LW, Li HM, Tang YJ	Bioprocess Biosyst Eng	10.1007/s00449-011-0644-6	2011	*
Phylogeny	Actinobacillus succinogenes sp. nov., a novel succinic-acid-producing strain from the bovine rumen.	Guettler MV, Rumler D, Jain MK	Int J Syst Bacteriol	10.1099/00207713-49-1-207	1999	*
	Succinic acid fermentation with immobilized Actinobacillus succinogenes using hydrolysate of carbohydrate-rich microalgal biomass.	Chiang YY, Nagarajan D, Lo YC, Chen CY, Ng IS, Chang CH, Lee DJ, Chang JS	Bioresour Technol	10.1016/j.biortech.2021.126014	2021	*
	Fermentation and crystallization of succinic acid from Actinobacillus succinogenes ATCC55618 using fresh cassava root as the main substrate.	Thuy NTH, Kongkaew A, Flood A, Boontawan A	Bioresour Technol	10.1016/j.biortech.2017.02.114	2017	*

References

#16239

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22257

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#41680 ; Curators of the CIP;
#56056 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 43843

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68377

Automatically annotated from API NH .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#80907

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID43634.1 )

#123367 Collection of Institut Pasteur ; Curators of the CIP; CIP 106512
* These data were automatically processed and therefore are not curated

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