Name: Halalkalibacterium halodurans PN 80
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1112

Type strain:

Species: Halalkalibacterium halodurans

Strain Designation: PN 80, B-3881

Culture col. no.: DSM 497, ATCC 27557, NRRL B-3881, CIP 105296

Strain history: CIP <- 1997, DSMZ <- NRRL: strain B-3881 <- E.W. Boyer: strain PN 80, Bacillus alcalophilus subsp. halodurans

NCBI tax ID(s): 86665 (species)

SI-ID 1092

LMG 7121, ATCC 27557, DSM 497, NRRL B-3881, CIP 105296, KCTC 3976

Other strains from Halalkalibacterium halodurans

Section

Halalkalibacterium halodurans PN 80 is an obligate aerobe, spore-forming, mesophilic bacterium that was isolated from dried sewage sludge.

motile
spore-forming
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Halalkalibacterium*
	Species	**Halalkalibacterium halodurans**
	Full Scientific Name (LPSN)	Halalkalibacterium halodurans (Nielsen et al. 1995 ex Boyer et al. 1973) Joshi et al. 2022

	Synonym	"Bacillus* alcalophilus subsp. halodurans"*
	Synonym	Bacillus okuhidensis
	Synonym	Bacillus halodurans
	Synonym	Alkalihalobacillus halodurans
	Synonym	Alkalihalobacillus okuhidensis

Information on morphological and physiological properties Morphology

[Ref.: #119803]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #119803]	Cell shape	rod-shaped

[Ref.: #119803]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %91.846

Information on culture and growth conditions Culture and growth conditions

[Ref.: #236]

Culture medium

ALKALINE NUTRIENT AGAR (DSMZ Medium 31)

[Ref.: #236]

Culture medium growth

[Ref.: #236]

Culture medium link

Medium recipe at MediaDive

[Ref.: #236]

Culture medium composition

expand / minimize

[Ref.: #38542]

Culture medium

MEDIUM 113 - for Bacillus

[Ref.: #38542]

Culture medium growth

[Ref.: #38542]

Culture medium composition

expand / minimize

[Ref.: #119803]

Culture medium

CIP Medium 113

[Ref.: #119803]

Culture medium growth

[Ref.: #119803]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #236]		growth	30 °C
[Ref.: #38542]		growth	30 °C
[Ref.: #119803]		growth	22-55 °C
[Ref.: #119803]	no	growth	10 °C

[Ref.: #236]	Temperature range	mesophilic
[Ref.: #38542]	Temperature range	mesophilic
[Ref.: #119803]	Temperature range	psychrophilic

	pH	Kind of pH		pH
[Ref.: #119803]		no	growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #119803]

Oxygen tolerance

obligate aerobe

[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

[Ref.: #236]

Name of produced compound

ß amylase, alkaline

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #119803]		NaCl		growth	2-10	%
[Ref.: #119803]		NaCl	no	growth	0	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #119803]	citrate ChEBI	-	carbon source
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #119803]	esculin ChEBI	-	hydrolysis
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #119803]	hippurate ChEBI	-	hydrolysis
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #119803]	nitrate ChEBI	-	reduction
[Ref.: #119803]	nitrate ChEBI	-	respiration
[Ref.: #119803]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #119803]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #119803]		acetoin ChEBI	-
[Ref.: #119803]		glucose ChEBI		+

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #119803]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #119803]	amylase	-
[Ref.: #119803]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #119803]	caseinase	-	3.4.21.50
[Ref.: #119803]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #119803]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #119803]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #119803]	gelatinase	+
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #119803]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119803]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #119803]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #119803]	tween esterase	-
[Ref.: #119803]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #119803]	10023	not determinedn.d.	+	-	-	-	+	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+	+/-	-	+	+	+	+	-	-	+/-	+	-	-	-	+/-	+	-	-	+	-	-	-	-	-	-	+/-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119803]	8594	-	-	+	+	-	-	-	-	-	-	-	+	-	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #236]	Sample type/isolated from	dried sewage sludge

[Ref.: #119803]	Sample type/isolated from	Environment, Dried sewage sludge
* marker position based on {}

Isolation sources categories

#Engineered

#Waste

#Sewage sludge

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ302709 (>99% sequence identity) for Alkalihalobacillus halodurans from Microbeatlas

Aquatic Samples	1519
Soil Samples	1545
Animal Samples	3493
Plant Samples	429
Total Samples	6986

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #236]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119803]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Bacillus halodurans gene for 16S ribosomal RNA	AB021187	1508	ENA	86665 tax ID	*
[Ref.: #20218]	Bacillus halodurans strain ATCC 27557 16S ribosomal RNA gene, partial sequence	KC884852	501	ENA	86665 tax ID	*
[Ref.: #20218]	Bacillus halodurans 16S rRNA gene, strain DSM 497T	AJ302709	1509	ENA	86665 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Halalkalibacterium halodurans DSM 497	GCA_004376175	scaffold	GenBank	86665 tax ID	*
[Ref.: #66792]	Bacillus halodurans strain DSM 497	86665.8	wgs	PATRIC	86665 tax ID	*

[Ref.: #236]

GC-content

42.5 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Alkalihalobacillus halodurans DSM 497 NCBI: GCA_004376175.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Alkalihalobacillus halodurans DSM 497 NCBI: GCA_004376175.1
[Ref.: #69480]	motile	yes	89.369	no
[Ref.: #69480]	flagellated	yes	81.891	no
[Ref.: #69480]	gram-positive	yes	85.442	no
[Ref.: #69480]	anaerobic	no	97.833	no
[Ref.: #69480]	aerobic	yes	82.74	no
[Ref.: #69480]	halophile	yes	71.292	no
[Ref.: #69480]	spore-forming	yes	95.012	no
[Ref.: #69480]	glucose-util	yes	90.458	no
[Ref.: #69480]	thermophile	no	92.512	yes
[Ref.: #69480]	glucose-ferment	no	92.457	no

Availability in culture collections External links

[Ref.: #236]

Culture collection no.

DSM 497, ATCC 27557, NRRL B-3881, CIP 105296

[Ref.: #70784]

SI-ID 1092

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Genome-based reclassification of Bacillus okuhidensis as a later heterotypic synonym of Bacillus halodurans.	Liu GH, Narsing Rao MP, Dong ZY, Wang JP, Che JM, Chen QQ, Sengonca C, Liu B, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.003666	2019	*
Phylogeny	Bacterial treatment of alkaline cement kiln dust using Bacillus halodurans strain KG1.	Kunal, Rajor A, Siddique R	Braz J Microbiol	10.1016/j.bjm.2015.11.001	2016	*
Phylogeny	Bacillus ligniniphilus sp. nov., an alkaliphilic and halotolerant bacterium isolated from sediments of the South China Sea.	Zhu D, Tanabe SH, Xie C, Honda D, Sun J, Ai L	Int J Syst Evol Microbiol	10.1099/ijs.0.058610-0	2014	*
Phylogeny	Starch hydrolysing Bacillus halodurans isolates from a Kenyan soda lake.	Hashim SO, Delgado O, Hatti-Kaul R, Mulaa FJ, Mattiasson B	Biotechnol Lett	10.1023/b:bile.0000025885.19910.d7	2004	*
Phylogeny	Revision of the taxonomic position of the xylanolytic Bacillus sp. MIR32 reidentified as Bacillus halodurans and plasmid-mediated transformation of B. halodurans.	Martinez MA, Delgado OD, Breccia JD, Baigori MD, Sineriz F	Extremophiles	10.1007/s00792-002-0269-4	2002	*
Phylogeny	Reidentification of the keratinase-producing facultatively alkaliphilic Bacillus sp. AH-101 as Bacillus halodurans.	Takami H, Nogi Y, Horikoshi K	Extremophiles	10.1007/s007920050130	1999	*
Phylogeny	Identification of Bacillus sp. FTU strain and the study of the caa3-type oxidase homology.	Grinkevich VA, Lysenko AM, Muntyan MS, Skripnikova EV, Afrikyan EK	Biochemistry (Mosc)		1997	*

References

#236	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 497

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#38542 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70784

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID1092.1 )

#119803 Collection of Institut Pasteur ; Curators of the CIP; CIP 105296
* These data were automatically processed and therefore are not curated

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Alkalihalobacillus halodurans DSM 497

Alkalihalobacillus halodurans DSM 497

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