Name: Rhodococcus phenolicus G2P
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 11042

Type strain:

Species: Rhodococcus phenolicus

Strain Designation: G2P

Culture col. no.: DSM 44812, CIP 109148, JCM 14914, NRRL B-24323

Strain history: CIP <- 2006, DSMZ <- M. Rehfuss: strain G2P

NCBI tax ID(s): 1303680 (strain), 263849 (species)

SI-ID 291003

DSM 44812, NRRL B-24323, CIP 109148, JCM 14914

Section

Rhodococcus phenolicus G2P is a spore-forming, mesophilic prokaryote that was isolated from biological wastewater processor.

spore-forming
mesophilic
16S sequence
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Genus	*Rhodococcus*
	Species	**Rhodococcus phenolicus**
	Full Scientific Name (LPSN)	Rhodococcus phenolicus Rehfuss and Urban 2006

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %99.999

[Ref.: #69480]	Motility	no Confidence in %91.117

Multimedia content

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Caption:

Medium 535 28°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

MEDIUM 72- for trypto casein soja agar

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Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

CIP Medium 72

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #12097]	growth	28 °C
[Ref.: #38383]	growth	30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #12097]	Temperature range	mesophilic
[Ref.: #38383]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	yes Confidence in %91
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.998

[Ref.: #12097]	Name of produced compound	phenol
[Ref.: #12097]	Name of produced compound	chlorobenzene
[Ref.: #12097]	Name of produced compound	dichlorobenzenes

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121795]	11683	-	+	+	+	-	+	-	-	-	-	+	-	-	-	-	-	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #12097]	Sample type/isolated from	biological wastewater processor
[Ref.: #12097]	Geographic location (country and/or sea, region)	Texas, Johnson Space Center
[Ref.: #12097]	Country	USA
[Ref.: #12097]	Country ISO 3 Code	USA
[Ref.: #12097]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Johnson Space Center graywater bioprocessor
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America

[Ref.: #121795]	Sample type/isolated from	Environment, Johson Space Center graywater bioprocessor
[Ref.: #121795]	Geographic location (country and/or sea, region)	Houston, Texas
[Ref.: #121795]	Country	United States of America
[Ref.: #121795]	Country ISO 3 Code	USA
[Ref.: #121795]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered	#Industrial	#Machines and devices
#Engineered	#Waste	#Wastewater

What are isolation sources categories?

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Global distribution of 16S sequence AM933579 (>99% sequence identity) for Rhodococcus from Microbeatlas

Aquatic Samples	349
Soil Samples	259
Animal Samples	272
Plant Samples	20
Total Samples	900

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #12097]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121795]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Rhodococcus phenolicus partial 16S rRNA gene, type strain DSM 44812T	AM933579	1511	ENA	263849 tax ID	*
[Ref.: #20218]	Rhodococcus phenolicus strain DSM 44812 16S ribosomal RNA gene, partial sequence	KF410354	1379	ENA	263849 tax ID	*
[Ref.: #20218]	Rhodococcus phenolicus 16S ribosomal RNA gene, partial sequence	AY533293	1395	ENA	263849 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Rhodococcus phenolicus DSM 44812	GCA_001646785	scaffold	GenBank	263849 tax ID
[Ref.: #66792]	Rhodococcus phenolicus JCM 14914	GCA_001313425	contig	GenBank	1303680 tax ID	*
[Ref.: #66792]	Rhodococcus phenolicus JCM 14914	1303680.3	wgs	PATRIC	1303680 tax ID	*
[Ref.: #66792]	Rhodococcus phenolicus strain DSM 44812	263849.4	wgs	PATRIC	263849 tax ID	*
[Ref.: #66792]	Rhodococcus phenolicus DSM 44812	2744054614	draft	JGI IMG/M	263849 tax ID	*
[Ref.: #66792]	Rhodococcus phenolicus JCM 14914	2675903364	draft	JGI IMG/M	1303680 tax ID	*

[Ref.: #12097]	GC-content	66.7 mol%
[Ref.: #67770]	GC-content	66.7 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	68.4 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Rhodococcus phenolicus JCM 14914 PATRIC: 1303680.3
[Ref.: #69481]	spore-forming	yes	91	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Rhodococcus phenolicus JCM 14914 PATRIC: 1303680.3
[Ref.: #69480]	motile	no	91.389	no
[Ref.: #69480]	gram-positive	yes	84.75	no
[Ref.: #69480]	anaerobic	no	98.047	no
[Ref.: #69480]	halophile	no	93.911	no
[Ref.: #69480]	spore-forming	no	90.865	no
[Ref.: #69480]	thermophile	no	99.644	yes
[Ref.: #69480]	glucose-util	yes	84.716	no
[Ref.: #69480]	aerobic	yes	88.878	no
[Ref.: #69480]	flagellated	no	98.452	no
[Ref.: #69480]	glucose-ferment	no	89.965	no

Availability in culture collections External links

[Ref.: #12097]

Culture collection no.

DSM 44812, CIP 109148, JCM 14914, NRRL B-24323

[Ref.: #80294]

SI-ID 291003

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Rhodococcus phenolicus sp. nov., a novel bioprocessor isolated actinomycete with the ability to degrade chlorobenzene, dichlorobenzene and phenol as sole carbon sources.	Rehfuss M, Urban J	Syst Appl Microbiol	10.1016/j.syapm.2005.05.011	2005	*

References

#12097

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44812

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#38383 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#80294

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID291003.1 )

#121795 Collection of Institut Pasteur ; Curators of the CIP; CIP 109148
* These data were automatically processed and therefore are not curated

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Rhodococcus phenolicus JCM 14914

Rhodococcus phenolicus JCM 14914

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